Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-oxoimidazolidinyl-4-carbonyl-Gly-Ile + H2O
?
-
-
-
-
?
4(R)-N-(2-nitroxyethyl)-2-oxothiazolidine-4-carboxamide + H2O
L-2-oxothiazolidine-4-carboxylic acid + ?
5-pyrrolidone-2-carboxyl-His-Gly + H2O
?
-
slight activity
-
-
?
5-pyrrolidone-2-carboxyl-His-Trp + H2O
?
-
-
-
-
?
ACE inhibitor + H2O
pyroglutamate + ?
contains the N-terminal dipeptide pyroglutamyl-Trp-
-
?
acid-thyroliberin + H2O
pyroglutamate + His-Pro
bombesin + H2O
pyroglutamate + ?
contains the N-terminal dipeptide pyroglutamyl-Arg-
-
?
bombesin + H2O
pyroglutamate + Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH2
bradykinin potentiator + H2O
pyroglutamate + ?
bradykinin potentiator B + H2O
pyroglutamate + ?
contains the N-terminal dipeptide pyroglutamyl-Gly-
-
?
bradykinin-potentiating peptide B + H2O
?
-
cleavage of the peptide bond adjacent to the pyroglutamic residue
-
-
?
ethyl L-pyroglutamate + H2O
L-pyroglutamate + ethanol
Fibrinogen + H2O
?
-
bovine fribrinogen
-
-
?
Glu(OMe)2-litorin + H2O
pyroglutamate + ?
-
-
-
?
gonadotropin-releasing hormone + H2O
?
hydrolyzed exclusively at the pyroglutamyl-/-Xaa bond
-
-
?
L-2-oxoimidazolidine-4-carboxyl-L-Ala + H2O
?
L-2-oxooxazolidine-4-carboxyl-aminoethylnitrate + H2O
?
-
-
-
-
?
L-2-oxooxazolidine-4-carboxyl-L-Ala + H2O
?
L-2-oxothiazolidine-4-carboxyl-D-2-aminopropylnitrate + H2O
?
-
-
-
-
?
L-2-oxothiazolidine-4-carboxyl-ethanolamine + H2O
?
-
-
-
-
?
L-2-oxothiazolidine-4-carboxyl-L-2-aminopropylnitrate + H2O
?
-
-
-
-
?
L-2-oxothiazolidine-4-carboxyl-L-Ala + H2O
?
L-pyroglutamic acid 2-naphthylamide + H2O
L-pyroglutamate + 2-naphthylamine
-
-
-
-
?
L-pyroglutamyl p-nitroanilide + H2O
L-pyroglutamate + p-nitroaniline
-
-
-
-
?
L-pyroglutamyl-7-amido-4-methylcoumarin + H2O
L-pyroglutamate + 7-amino-4-methylcoumarin
-
-
-
?
L-pyroglutamyl-aminoethylnitrate + H2O
?
-
-
-
-
?
L-pyroglutamyl-beta-naphthylamide + H2O
L-pyroglutamate + 2-naphthylamine
L-pyroglutamyl-beta-naphthylamide + H2O
L-pyroglutamate + 2-naphtylamine
-
-
-
-
?
L-pyroglutamyl-beta-naphthylamide + H2O
L-pyroglutamate + beta-naphthylamine
L-pyroglutamyl-beta-naphthylamide + H2O
L-pyroglutamate + beta-naphtylamine
-
-
-
-
?
L-pyroglutamyl-His-Pro-4-methylcoumarin 7-amide + H2O
L-pyroglutamate + His-Pro-4-methylcoumarin 7-amide
-
-
-
-
?
L-pyroglutamyl-L-Ala + H2O
L-pyroglutamate + L-Ala
L-pyroglutamyl-L-Ile + H2O
L-pyroglutamate + L-Ile
-
-
-
-
?
L-pyroglutamyl-L-Thr + H2O
L-pyroglutamate + L-Thr
-
-
-
-
?
L-pyroglutamyl-p-nitroanilide + H2O
L-pyroglutamate + p-nitroaniline
L-pyroglutamyl-p-nitroanilide + H2O
L-pyroglutaminic acid + p-nitroaniline
-
-
-
?
L-pyrrolidonyl 2-naphthylamide + H2O
5-oxo-L-proline + 2-naphthylamine
-
-
-
-
?
leukopyrokinin + H2O
pyroglutamate + ?
contains the N-terminal dipeptide pyroglutamyl-Thr-
-
?
litorin + H2O
pyroglutamate + Gln-Trp-Ala-Val-Gly-His-Phe-Met-NH2
luliberin + H2O
pyroglutamate + His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
luteinizing hormone releasing hormone + H2O
pyroglutamate + ?
contains the N-terminal dipeptide pyroglutamyl-His-
-
?
luteinizing-hormone-releasing hormone + H2O
?
N-(4-methyl-2-oxo-2H-chromen-7-yl)-5-oxo-L-prolinamide + H2O
5-oxo-L-proline + 7-amino-4-methylcoumarin
-
-
-
-
?
neurotensin + H2O
pyroglutamate + ?
contains the N-terminal dipeptide pyroglutamyl-Leu-
-
?
neurotensin + H2O
pyroglutamate + Leu-Tyr-Glu-Asn-Lys-Pro-Ag-Arg-Pro-Tyr-Ile-Leu
physalaemin + H2O
pyroglutamate + Ala-Asp-Pro-Asn-Lys-Phe-Tyr-Gly-Leu-Met-NH2
pyroglutamic acid-7-amido-4-methylcoumarin + H2O
L-pyroglutamate + 7-amino-4-methylcoumarin
-
-
-
-
?
pyroglutamyl-2-naphthylamide + H2O
pyroglutamate + 2-naphthylamine
-
-
-
-
?
pyroglutamyl-4-methylcoumarin 7-amide + H2O
pyroglutamate + 7-amino-4-methylcoumarin
pyroglutamyl-4-methylcoumaryl-7-amide + H2O
pyroglutamate + 7-amino-4-methylcoumarin
-
-
?
pyroglutamyl-4-methylumbelliferyl ester + H2O
?
-
-
-
-
?
pyroglutamyl-Ala + H2O
pyroglutamate + Ala
pyroglutamyl-Arg + H2O
pyroglutamate + Arg
-
-
-
-
?
pyroglutamyl-Asp + H2O
pyroglutamate + Asp
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
pyroglutamyl-D-Ala + H2O
pyroglutamate + D-Ala
pyroglutamyl-D-Leu + H2O
pyroglutamate + D-Leu
-
-
-
-
?
pyroglutamyl-Glu + H2O
pyroglutamate + Glu
-
-
-
-
?
pyroglutamyl-Gly + H2O
pyroglutamate + Gly
-
-
-
-
?
pyroglutamyl-Gly-Ile + H2O
pyroglutamate + Gly-Ile
-
-
-
-
?
pyroglutamyl-His + H2O
pyroglutamate + His
-
-
-
-
?
pyroglutamyl-His-Gly + H2O
pyroglutamate + His-Gly
pyroglutamyl-His-Gly-NH2 + H2O
pyroglutamate + His-Gly-NH2
pyroglutamyl-His-Pro-Gly + H2O
pyroglutamate + His-Pro-Gly
-
-
-
-
?
pyroglutamyl-His-Pro-Gly-NH2 + H2O
pyroglutamate + His-Pro-Gly-NH2
-
-
-
-
?
pyroglutamyl-Ile + H2O
pyroglutamate + Ile
-
-
-
-
?
pyroglutamyl-Leu + H2O
pyroglutamate + Leu
-
-
-
-
?
pyroglutamyl-Lys + H2O
pyroglutamate + Lys
-
-
-
-
?
pyroglutamyl-Met + H2O
pyroglutamate + Met
-
-
-
-
?
pyroglutamyl-p-nitroanilide + H2O
pyroglutamate + p-nitroaniline
pyroglutamyl-peptide + H2O
pyroglutamate + peptide
-
the enzyme is able to specifically remove the amino-terminal pyroglutamyl residue protecting proteins or peptides from aminopeptidases
-
?
pyroglutamyl-Phe + H2O
pyroglutamate + Phe
-
-
-
-
?
pyroglutamyl-Phe-Pro-NH2 + H2O
pyroglutamate + Phe-Pro-NH2
-
-
-
-
?
pyroglutamyl-Ser + H2O
pyroglutamate + Ser
-
-
-
-
?
pyroglutamyl-Thr + H2O
pyroglutamate + Thr
-
-
-
-
?
pyroglutamyl-Trp + H2O
pyroglutamate + Trp
-
-
-
-
?
pyroglutamyl-Tyr + H2O
pyroglutamate + Tyr
-
-
-
-
?
pyroglutamyl-Val + H2O
pyroglutamate + Val
-
-
-
-
?
thyroliberin + H2O
pyroglutamate + His-Pro-NH2
thyroliberin-releasing hormone + H2O
?
-
-
-
-
?
thyrotrophin releasing hormone
?
thyrotrophin-releasing hormone + H2O
?
thyrotropin-releasing hormone + H2O
?
additional information
?
-
4(R)-N-(2-nitroxyethyl)-2-oxothiazolidine-4-carboxamide + H2O

L-2-oxothiazolidine-4-carboxylic acid + ?
-
-
-
-
?
4(R)-N-(2-nitroxyethyl)-2-oxothiazolidine-4-carboxamide + H2O
L-2-oxothiazolidine-4-carboxylic acid + ?
-
-
-
-
?
4(R)-N-(2-nitroxyethyl)-2-oxothiazolidine-4-carboxamide + H2O
L-2-oxothiazolidine-4-carboxylic acid + ?
-
-
-
-
?
acid-thyroliberin + H2O

pyroglutamate + His-Pro
-
-
-
-
?
acid-thyroliberin + H2O
pyroglutamate + His-Pro
-
i.e. pyroglutamyl-His-Pro
-
?
bombesin + H2O

pyroglutamate + Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH2
-
-
-
-
?
bombesin + H2O
pyroglutamate + Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH2
-
i.e. pyroglutamyl-Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH2
-
?
bradykinin potentiator + H2O

pyroglutamate + ?
-
-
-
-
?
bradykinin potentiator + H2O
pyroglutamate + ?
-
-
-
?
ethyl L-pyroglutamate + H2O

L-pyroglutamate + ethanol
-
-
-
?
ethyl L-pyroglutamate + H2O
L-pyroglutamate + ethanol
-
-
-
?
L-2-oxoimidazolidine-4-carboxyl-L-Ala + H2O

?
-
-
-
-
?
L-2-oxoimidazolidine-4-carboxyl-L-Ala + H2O
?
-
-
-
-
?
L-2-oxoimidazolidine-4-carboxyl-L-Ala + H2O
?
-
-
-
-
?
L-2-oxooxazolidine-4-carboxyl-L-Ala + H2O

?
-
-
-
-
?
L-2-oxooxazolidine-4-carboxyl-L-Ala + H2O
?
-
-
-
-
?
L-2-oxooxazolidine-4-carboxyl-L-Ala + H2O
?
-
-
-
-
?
L-2-oxothiazolidine-4-carboxyl-L-Ala + H2O

?
-
-
-
-
?
L-2-oxothiazolidine-4-carboxyl-L-Ala + H2O
?
-
-
-
-
?
L-2-oxothiazolidine-4-carboxyl-L-Ala + H2O
?
-
-
-
-
?
L-pyroglutamyl-beta-naphthylamide + H2O

L-pyroglutamate + 2-naphthylamine
-
-
-
?
L-pyroglutamyl-beta-naphthylamide + H2O
L-pyroglutamate + 2-naphthylamine
-
-
-
?
L-pyroglutamyl-beta-naphthylamide + H2O

L-pyroglutamate + beta-naphthylamine
-
-
-
?
L-pyroglutamyl-beta-naphthylamide + H2O
L-pyroglutamate + beta-naphthylamine
-
-
-
?
L-pyroglutamyl-beta-naphthylamide + H2O
L-pyroglutamate + beta-naphthylamine
-
-
-
?
L-pyroglutamyl-beta-naphthylamide + H2O
L-pyroglutamate + beta-naphthylamine
-
-
-
?
L-pyroglutamyl-beta-naphthylamide + H2O
L-pyroglutamate + beta-naphthylamine
-
-
-
?
L-pyroglutamyl-L-Ala + H2O

L-pyroglutamate + L-Ala
-
-
-
-
?
L-pyroglutamyl-L-Ala + H2O
L-pyroglutamate + L-Ala
-
-
-
-
?
L-pyroglutamyl-L-Ala + H2O
L-pyroglutamate + L-Ala
-
-
-
-
?
L-pyroglutamyl-L-Ala + H2O
L-pyroglutamate + L-Ala
-
-
-
-
?
L-pyroglutamyl-p-nitroanilide + H2O

L-pyroglutamate + p-nitroaniline
-
-
-
?
L-pyroglutamyl-p-nitroanilide + H2O
L-pyroglutamate + p-nitroaniline
-
-
-
-
?
L-pyroglutamyl-p-nitroanilide + H2O
L-pyroglutamate + p-nitroaniline
-
-
-
?
L-pyroglutamyl-p-nitroanilide + H2O
L-pyroglutamate + p-nitroaniline
-
-
-
?
L-pyroglutamyl-p-nitroanilide + H2O
L-pyroglutamate + p-nitroaniline
-
-
-
?
litorin + H2O

pyroglutamate + Gln-Trp-Ala-Val-Gly-His-Phe-Met-NH2
-
frog litorin
-
?
litorin + H2O
pyroglutamate + Gln-Trp-Ala-Val-Gly-His-Phe-Met-NH2
-
frog litorin
-
?
luliberin + H2O

pyroglutamate + His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
-
i.e. pyroglutamyl-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
-
?
luliberin + H2O
pyroglutamate + His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
-
-
-
-
?
luteinizing-hormone-releasing hormone + H2O

?
-
-
-
-
?
luteinizing-hormone-releasing hormone + H2O
?
-
-
-
-
?
luteinizing-hormone-releasing hormone + H2O
?
-
-
-
-
?
neurotensin + H2O

pyroglutamate + Leu-Tyr-Glu-Asn-Lys-Pro-Ag-Arg-Pro-Tyr-Ile-Leu
-
-
-
-
?
neurotensin + H2O
pyroglutamate + Leu-Tyr-Glu-Asn-Lys-Pro-Ag-Arg-Pro-Tyr-Ile-Leu
-
-
-
-
?
neurotensin + H2O
pyroglutamate + Leu-Tyr-Glu-Asn-Lys-Pro-Ag-Arg-Pro-Tyr-Ile-Leu
-
i.e. pyroglutamyl-Leu-Tyr-Glu-Asn-Lys-Pro-Ag-Arg-Pro-Tyr-Ile-Leu
-
?
neurotensin + H2O
pyroglutamate + Leu-Tyr-Glu-Asn-Lys-Pro-Ag-Arg-Pro-Tyr-Ile-Leu
-
-
-
-
?
neurotensin + H2O
pyroglutamate + Leu-Tyr-Glu-Asn-Lys-Pro-Ag-Arg-Pro-Tyr-Ile-Leu
-
-
-
-
?
physalaemin + H2O

pyroglutamate + Ala-Asp-Pro-Asn-Lys-Phe-Tyr-Gly-Leu-Met-NH2
-
-
-
?
physalaemin + H2O
pyroglutamate + Ala-Asp-Pro-Asn-Lys-Phe-Tyr-Gly-Leu-Met-NH2
-
-
-
?
pyroglutamyl-4-methylcoumarin 7-amide + H2O

pyroglutamate + 7-amino-4-methylcoumarin
-
-
-
-
?
pyroglutamyl-4-methylcoumarin 7-amide + H2O
pyroglutamate + 7-amino-4-methylcoumarin
-
-
-
-
?
pyroglutamyl-4-methylcoumarin 7-amide + H2O
pyroglutamate + 7-amino-4-methylcoumarin
-
-
-
-
?
pyroglutamyl-Ala + H2O

pyroglutamate + Ala
-
-
-
-
?
pyroglutamyl-Ala + H2O
pyroglutamate + Ala
-
-
-
-
?
pyroglutamyl-Ala + H2O
pyroglutamate + Ala
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O

pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
no activity in Staphylococcus aureus
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
no activity in Staphylococcus aureus ATCC 25923
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
no activity in Staphylococcus pseudintermedius
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
no activity in Staphylococcus pseudintermedius LMG 22219T
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-beta-naphthylamide + H2O
pyroglutamate + beta-naphthylamine
-
-
-
-
?
pyroglutamyl-D-Ala + H2O

pyroglutamate + D-Ala
-
-
-
-
?
pyroglutamyl-D-Ala + H2O
pyroglutamate + D-Ala
-
slight activity
-
-
?
pyroglutamyl-His-Gly + H2O

pyroglutamate + His-Gly
-
-
-
-
?
pyroglutamyl-His-Gly + H2O
pyroglutamate + His-Gly
-
-
-
-
?
pyroglutamyl-His-Gly + H2O
pyroglutamate + His-Gly
-
i.e. anorexogenic peptide
-
?
pyroglutamyl-His-Gly-NH2 + H2O

pyroglutamate + His-Gly-NH2
-
-
-
-
?
pyroglutamyl-His-Gly-NH2 + H2O
pyroglutamate + His-Gly-NH2
-
-
-
-
?
pyroglutamyl-p-nitroanilide + H2O

pyroglutamate + p-nitroaniline
-
-
-
-
?
pyroglutamyl-p-nitroanilide + H2O
pyroglutamate + p-nitroaniline
-
-
-
-
?
thyroliberin + H2O

pyroglutamate + His-Pro-NH2
-
-
-
-
?
thyroliberin + H2O
pyroglutamate + His-Pro-NH2
-
-
-
-
?
thyroliberin + H2O
pyroglutamate + His-Pro-NH2
-
removes the N-terminal pyroglutamic acid
-
-
?
thyroliberin + H2O
pyroglutamate + His-Pro-NH2
-
i.e. pyroglutamyl-His-Pro-NH2, i.e. thyrotrophin releasing hormone
-
?
thyroliberin + H2O
pyroglutamate + His-Pro-NH2
-
-
-
-
?
thyroliberin + H2O
pyroglutamate + His-Pro-NH2
-
-
-
-
?
thyroliberin + H2O
pyroglutamate + His-Pro-NH2
-
-
-
?
thyroliberin + H2O
pyroglutamate + His-Pro-NH2
-
-
-
-
?
thyrotrophin releasing hormone

?
-
the enzyme is proposed to inactivate thyrotrophin releasing hormone in brain extracellular space
-
-
?
thyrotrophin releasing hormone
?
-
-
-
-
?
thyrotrophin-releasing hormone + H2O

?
trypanosomal pyroglutamyl aminopeptidase I is responsible for the abnormally rapid degradation of thyrotrophin-releasing hormone in the circulation of infected rats
-
-
?
thyrotrophin-releasing hormone + H2O
?
hydrolyzed exclusively at the pyroglutamyl-/-Xaa bond
-
-
?
thyrotropin-releasing hormone + H2O

?
-
-
-
?
thyrotropin-releasing hormone + H2O
?
-
-
-
?
thyrotropin-releasing hormone + H2O
?
-
-
-
-
?
thyrotropin-releasing hormone + H2O
?
-
-
-
?
additional information

?
-
-
slight activity toward L-pyroglutamyl-D-amino acids, inert toward L-pyroglutamyl-L-Pro
-
-
?
additional information
?
-
-
the molecular recognition of pyroglutamic acid is achieved through two hydrogen bonds and an insertion in the hydrophobic pocket. In the pocket Phe10 is more important to the hydrophobic interaction than is Phe142 and furthermore Phe13 serves as an induced fit mechanism
-
?
additional information
?
-
-
the enzyme does not catalyze the hydrolysis of peptides with six-membered ureido rings at the amino terminus, but it tolerates well a five-membered ureido ring at this position
-
-
?
additional information
?
-
-
the enzyme appears to play an important role in the activation and inactivation of many N-terminal pyroglutamyl-terminating peptides
-
-
?
additional information
?
-
-
no hydrolysis of dipeptides containing N-terminal pyroglutamic acid or with pyroglutamyl peptides containing more than three amino acids
-
-
?
additional information
?
-
-
the enzyme appears to play an important role in the activation and inactivation of many N-terminal pyroglutamyl-terminating peptides
-
-
?
additional information
?
-
-
the enzyme appears to play an important role in the activation and inactivation of many N-terminal pyroglutamyl-terminating peptides
-
-
?
additional information
?
-
-
the enzyme appears to play an important role in the activation and inactivation of many N-terminal pyroglutamyl-terminating peptides
-
-
?
additional information
?
-
-
the enzyme is inactive towards uperolein and piperidone carbonyl His-Pro-NH2
-
-
?
additional information
?
-
-
the enzyme appears to play an important role in the activation and inactivation of many N-terminal pyroglutamyl-terminating peptides
-
-
?
additional information
?
-
-
enzyme may participate in regulating the levels of seminal thyrotropin-relasing hormone analogues and in mediating sperm death associated with necrozoospermia
-
-
?
additional information
?
-
-
the enzyme appears to play an important role in the activation and inactivation of many N-terminal pyroglutamyl-terminating peptides
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
pyroglutamate and iron regulate the expression of the enzyme
-
-
?
additional information
?
-
-
pyroglutamate and iron regulate the expression of the enzyme
-
-
?
additional information
?
-
-
PGAP performs enzymatic deblocking of pyroglutamylated immunoglobulins. Unfolding of immunoglobulins is required for excision of the pyroglutamate. The enzyme requires the substrate in an extended conformation, which is not fullfiled in the native form of immunoglobulins
-
-
?
additional information
?
-
-
the enzyme appears to play an important role in the activation and inactivation of many N-terminal pyroglutamyl-terminating peptides
-
-
?
additional information
?
-
-
diurnal variation and left-right distribution of pyroglutamyl peptidase I activity in the rat brain and retina
-
-
?
additional information
?
-
-
pyroglutamyl peptidase I levels and their left-right distribution in the rat retina and hypothalamus are influenced by light-dark conditions
-
-
?
additional information
?
-
the enzyme might not be dominantly involved in the degradation of thyrotropin-releasing hormone in rats
-
-
?
additional information
?
-
-
the enzyme appears to play an important role in the activation and inactivation of many N-terminal pyroglutamyl-terminating peptides
-
-
?
additional information
?
-
-
the enzyme appears to play an important role in the activation and inactivation of many N-terminal pyroglutamyl-terminating peptides
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Coinfection
Mucosal T cells recovered from mice after infection with respiratory syncytial virus display a memory/activation phenotype.
Cystitis
Inflammation and nerve injury induce expression of pancreatitis-associated protein-II in primary sensory neurons.
Dermatitis, Atopic
Hemopoietic progenitor cells in atopic dermatitis skin lesions.
Endotoxemia
Pancreatitis-associated protein-I mRNA expression in mouse pancreas is upregulated by lipopolysaccharide independent of cerulein-pancreatitis.
Hyperalgesia
Nerve Injury-Induced Neuronal PAP-I Maintains Neuropathic Pain by Activating Spinal Microglia.
Hyperthyroidism
Thyroid status regulates particulate but not soluble TRH-degrading pyroglutamate aminopeptidase activity in the rat liver.
Infections
CD44 is not required for poliovirus replication.
Infections
Evaluation of three disk tests for identification of enterococci, leuconostocs, and pediococci.
Infections
Mucosal T cells recovered from mice after infection with respiratory syncytial virus display a memory/activation phenotype.
Influenza, Human
Comparative behavior of membrane protein-antibody complexes on motile fibroblasts: implications for a mechanism of capping.
Insulinoma
Evidence for pyroglutamyl peptidase I and prolyl endopeptidase activities in the rat insulinoma cell line RINm 5F: lack of relationship with TRH metabolism.
Kidney Neoplasms
Altered levels of acid, basic, and neutral peptidase activity and expression in human clear cell renal cell carcinoma.
Leukemia
Hepatic gene expression patterns following trauma-hemorrhage: effect of posttreatment with estrogen.
Leukemia
High cellular accumulation of sulphoraphane, a dietary anticarcinogen, is followed by rapid transporter-mediated export as a glutathione conjugate.
Liver Cirrhosis
Serum pyroglutamyl aminopeptidase activity: a promising novel biomarker candidate for liver cirrhosis.
Lymphoma
Regulation of Thy-1 and Pgp-1 glycoproteins in hybrids between T-cell lymphomas and Abelson-leukemia-virus-induced lymphomas.
Lymphoma
T-cell lymphomas emerging as epineoplasms in mice bearing transplanted polyoma virus-induced salivary gland tumors.
Lymphoma, T-Cell
Regulation of Thy-1 and Pgp-1 glycoproteins in hybrids between T-cell lymphomas and Abelson-leukemia-virus-induced lymphomas.
Neoplasms
A ratiometric fluorescent sensor for rapid detection of the pyroglutamate aminopeptidase-1 in mouse tumors.
Neoplasms
Activity of the Bcl-2 family inhibitor ABT-263 in a panel of small cell lung cancer xenograft models.
Neoplasms
Altered Activity and Expression of Cytosolic Peptidases in Colorectal Cancer.
Neoplasms
Antigenic variation and macrophage infiltration of human bladder tumors xenografted into nude mice.
Neoplasms
Antitumor activity and tumor necrosis factor production of Phytolacca acinosa polysaccharides I in mice.
Neoplasms
Effects of Phytolacca acinosa polysaccharides I combined with interleukin-2 on the cytotoxicity of murine splenocytes against tumor cells.
Neoplasms
Moderate Beer Consumption Modifies Tumoral Growth Parameters and Pyrrolidone Carboxypeptidase Type-I and Type-II Specific Activities in the Hypothalamus-Pituitary-Mammary Gland Axis in an Animal Model of Breast Cancer.
Neoplasms
Physalaemin: an amphibian tachykinin in human lung small-cell carcinoma.
Neuralgia
Erratum: Li et al., 'Nerve Injury-Induced Neuronal PAP-I Maintains Neuropathic Pain by Activating Spinal Microglia'.
Neuralgia
Nerve Injury-Induced Neuronal PAP-I Maintains Neuropathic Pain by Activating Spinal Microglia.
Neuroblastoma
CD44 is not required for poliovirus replication.
Pancreatitis
Pancreatitis-associated protein-I and pancreatitis-associated protein-III expression in a rat model of kainic acid-induced seizure.
Pancreatitis
Pancreatitis-associated protein-I mRNA expression in mouse pancreas is upregulated by lipopolysaccharide independent of cerulein-pancreatitis.
Pancreatitis
Regulation of mouse pancreatitis-associated protein-I gene expression during caerulein-induced acute pancreatitis.
Peripheral Nerve Injuries
Inflammation and nerve injury induce expression of pancreatitis-associated protein-II in primary sensory neurons.
Peripheral Nerve Injuries
Nerve Injury-Induced Neuronal PAP-I Maintains Neuropathic Pain by Activating Spinal Microglia.
Prostatic Neoplasms
Discovery of 2-chloro-N-(4-methoxyphenyl)-N-methylquinazolin-4-amine (EP128265, MPI-0441138) as a potent inducer of apoptosis with high in vivo activity.
pyroglutamyl-peptidase i deficiency
Nerve Injury-Induced Neuronal PAP-I Maintains Neuropathic Pain by Activating Spinal Microglia.
Seizures
Pancreatitis-associated protein-I and pancreatitis-associated protein-III expression in a rat model of kainic acid-induced seizure.
Sepsis
Pseudomonas pneumonia-mediated sepsis induces expression of pancreatitis-associated protein-I in rat pancreas.
Tonsillitis
Activity of soluble aminopeptidase A and dipeptidyl peptidase IV and membrane-bound aminopeptidase B and pyroglutamyl peptidase I in adenoid hyperplasia, tonsillar hyperplasia and chronic tonsillitis.
Urinary Bladder Neoplasms
Antigenic variation and macrophage infiltration of human bladder tumors xenografted into nude mice.
Vesicular Stomatitis
Specific selection of host cell glycoproteins during assembly of murine leukaemia virus and vesicular stomatitis virus: presence of Thy-1 glycoprotein and absence of H-2, Pgp-1 and T-200 glycoproteins on the envelopes of these virus particles.
Virus Diseases
Intrahepatic alpha beta-TcRintermediate LFA-1high T cells are stimulated during mouse hepatitis viral infection.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.5 - 0.58
4(R)-N-(2-nitroxyethyl)-2-oxothiazolidine-4-carboxamide
0.079 - 0.089
gonadotropin-releasing hormone
0.33 - 0.42
L-2-oxoimidazolidine-4-carboxyl-L-Ala
1.53
L-2-oxooxazolidine-4-carboxyl-aminoethylnitrate
-
37°C, pH 7.4
0.55 - 0.71
L-2-oxooxazolidine-4-carboxyl-L-Ala
1.43
L-2-oxothiazolidine-4-carboxyl-D-2-aminopropylnitrate
-
37°C, pH 7.4
0.62
L-2-oxothiazolidine-4-carboxyl-ethanolamine
-
37°C, pH 7.4
0.57
L-2-oxothiazolidine-4-carboxyl-L-2-aminopropylnitrate
-
37°C, pH 7.4
0.3 - 0.43
L-2-oxothiazolidine-4-carboxyl-L-Ala
0.0335
L-pyroglutamyl p-nitroanilide
-
-
0.018 - 0.028
L-pyroglutamyl-7-amido-4-methylcoumarin
0.25
L-pyroglutamyl-Ala
-
-
0.14
L-pyroglutamyl-aminoethylnitrate
-
37°C, pH 7.4
0.29
L-pyroglutamyl-Arg
-
-
0.29
L-pyroglutamyl-Asp
-
-
0.17 - 1.46
L-pyroglutamyl-beta-naphthylamide
0.23
L-pyroglutamyl-Glu
-
-
0.2
L-pyroglutamyl-Gly
-
-
0.23
L-pyroglutamyl-His
-
-
0.045 - 0.89
L-pyroglutamyl-L-Ala
0.14
L-pyroglutamyl-L-Ile
-
-
-
0.24
L-pyroglutamyl-L-Thr
-
-
-
0.14
L-pyroglutamyl-Leu
-
-
0.2
L-pyroglutamyl-Lys
-
-
0.13
L-pyroglutamyl-Met
-
-
0.028 - 0.054
L-pyroglutamyl-p-nitroanilide
0.1
L-pyroglutamyl-Phe
-
-
0.15
L-pyroglutamyl-Ser
-
-
0.11
L-pyroglutamyl-Trp
-
-
0.2
L-pyroglutamyl-Tyr
-
-
0.09
L-pyroglutamyl-Val
-
-
0.49
luteinizing-hormone-releasing hormone
-
-
0.04 - 0.15
pyroglutamate-4-methylcoumarin 7-amide
0.059
pyroglutamic acid-7-amido-4-methylcoumarin
-
-
0.7
pyroglutamyl-2-naphthylamide
-
-
0.01536
pyroglutamyl-4-methylcoumarin 7-amide
-
-
0.05
pyroglutamyl-4-methylcoumaryl-7-amide
pH 7.8, 37°C
0.02076 - 1.79
Pyroglutamyl-beta-naphthylamide
0.00253 - 0.4198
pyroglutamyl-His-Pro-4-methylcoumarin 7-amide
0.0449
pyroglutamyl-His-Pro-NH2
-
-
0.73 - 20
pyroglutamyl-p-nitroanilide
0.04 - 0.727
Thyroliberin
0.59
thyroliberin-releasing hormone
-
-
-
0.05
thyrotrophic hormone releasing hormone
-
-
0.032 - 0.041
thyrotrophin-releasing hormone
0.041 - 0.056
thyrotropin-releasing hormone
additional information
additional information
-
-
-
0.5
4(R)-N-(2-nitroxyethyl)-2-oxothiazolidine-4-carboxamide

-
37°C, pH 7.4
0.55
4(R)-N-(2-nitroxyethyl)-2-oxothiazolidine-4-carboxamide
-
37°C, pH 7.4
0.58
4(R)-N-(2-nitroxyethyl)-2-oxothiazolidine-4-carboxamide
-
37°C, pH 7.4
0.079
gonadotropin-releasing hormone

37°C, pH 7.4, native enzyme
0.089
gonadotropin-releasing hormone
37°C, pH 7.4, recombinant enzyme
0.33
L-2-oxoimidazolidine-4-carboxyl-L-Ala

-
37°C, pH 7.4
0.37
L-2-oxoimidazolidine-4-carboxyl-L-Ala
-
37°C, pH 7.4
0.42
L-2-oxoimidazolidine-4-carboxyl-L-Ala
-
37°C, pH 7.4
0.55
L-2-oxooxazolidine-4-carboxyl-L-Ala

-
37°C, pH 7.4
0.57
L-2-oxooxazolidine-4-carboxyl-L-Ala
-
37°C, pH 7.4
0.71
L-2-oxooxazolidine-4-carboxyl-L-Ala
-
37°C, pH 7.4
0.3
L-2-oxothiazolidine-4-carboxyl-L-Ala

-
37°C, pH 7.4
0.33
L-2-oxothiazolidine-4-carboxyl-L-Ala
-
37°C, pH 7.4
0.43
L-2-oxothiazolidine-4-carboxyl-L-Ala
-
37°C, pH 7.4
0.018
L-pyroglutamyl-7-amido-4-methylcoumarin

37°C, pH 7.4, native enzyme
0.028
L-pyroglutamyl-7-amido-4-methylcoumarin
37°C, pH 7.4, recombinant enzyme
0.17
L-pyroglutamyl-beta-naphthylamide

-
pH 7.0, 37°C, mutant enzyme F142Y
0.18
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, mutant enzyme C68S/S185C
0.18
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, mutant enzyme S185C
0.2
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, mutant enzyme F13Y
0.21
L-pyroglutamyl-beta-naphthylamide
-
-
0.21
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, wild-type enzyme
0.25
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, wild-type enzyme
0.55
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, mutant enzyme F142A
0.76
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, mutant enzyme F10Y
1.46
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, mutant enzyme F142Y
0.045
L-pyroglutamyl-L-Ala

-
37°C, pH 7.4
0.046
L-pyroglutamyl-L-Ala
-
37°C, pH 7.4
0.057
L-pyroglutamyl-L-Ala
-
37°C, pH 7.4
0.89
L-pyroglutamyl-L-Ala
-
-
0.028
L-pyroglutamyl-p-nitroanilide

37°C, pH 7.4, native enzyme
0.038
L-pyroglutamyl-p-nitroanilide
37°C, pH 7.4
0.038
L-pyroglutamyl-p-nitroanilide
37°C, pH 7.4
0.044
L-pyroglutamyl-p-nitroanilide
37°C, pH 7.4, recombinant enzyme
0.054
L-pyroglutamyl-p-nitroanilide
37°C, pH 7.4
0.04
pyroglutamate-4-methylcoumarin 7-amide

-
-
0.08
pyroglutamate-4-methylcoumarin 7-amide
-
-
0.15
pyroglutamate-4-methylcoumarin 7-amide
-
-
0.02076
Pyroglutamyl-beta-naphthylamide

-
-
0.05
Pyroglutamyl-beta-naphthylamide
-
mutant enzyme D89N
0.21
Pyroglutamyl-beta-naphthylamide
-
wild-type enzyme
0.22
Pyroglutamyl-beta-naphthylamide
-
mutant enzyme E22Q
0.24
Pyroglutamyl-beta-naphthylamide
-
mutant enzyme E10Q
1.04
Pyroglutamyl-beta-naphthylamide
-
-
1.79
Pyroglutamyl-beta-naphthylamide
-
-
0.00253
pyroglutamyl-His-Pro-4-methylcoumarin 7-amide

-
-
0.01364
pyroglutamyl-His-Pro-4-methylcoumarin 7-amide
-
-
0.4198
pyroglutamyl-His-Pro-4-methylcoumarin 7-amide
-
-
0.73
pyroglutamyl-p-nitroanilide

-
-
20
pyroglutamyl-p-nitroanilide
-
-
0.04
Thyroliberin

-
-
0.032
thyrotrophin-releasing hormone

37°C, pH 7.4, recombinant enzyme
0.041
thyrotrophin-releasing hormone
37°C, pH 7.4, native enzyme
0.041
thyrotropin-releasing hormone

37°C, pH 7.4
0.046
thyrotropin-releasing hormone
37°C, pH 7.4
0.056
thyrotropin-releasing hormone
37°C, pH 7.4
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.8 - 2.3
4(R)-N-(2-nitroxyethyl)-2-oxothiazolidine-4-carboxamide
9 - 12
gonadotropin-releasing hormone
2.4 - 3.6
L-2-oxoimidazolidine-4-carboxyl-L-Ala
7.6
L-2-oxooxazolidine-4-carboxyl-aminoethylnitrate
-
37°C, pH 7.4
6.4 - 8.1
L-2-oxooxazolidine-4-carboxyl-L-Ala
1.5
L-2-oxothiazolidine-4-carboxyl-D-2-aminopropylnitrate
-
37°C, pH 7.4
0.9
L-2-oxothiazolidine-4-carboxyl-ethanolamine
-
37°C, pH 7.4
3.2
L-2-oxothiazolidine-4-carboxyl-L-2-aminopropylnitrate
-
37°C, pH 7.4
2.3 - 3.4
L-2-oxothiazolidine-4-carboxyl-L-Ala
47 - 58
L-pyroglutamyl-7-amido-4-methylcoumarin
3.5
L-pyroglutamyl-aminoethylnitrate
-
37°C, pH 7.4
0.5 - 785
L-pyroglutamyl-beta-naphthylamide
2.4 - 3.7
L-pyroglutamyl-L-Ala
52 - 64
L-pyroglutamyl-p-nitroanilide
7.2
luteinizing-hormone-releasing hormone
-
-
3.5
pyroglutamic acid-7-amido-4-methylcoumarin
-
-
7.4 - 35.1
Pyroglutamyl-beta-naphthylamide
21
thyroliberin-releasing hormone
-
-
-
20 - 22
thyrotrophin-releasing hormone
1.8
4(R)-N-(2-nitroxyethyl)-2-oxothiazolidine-4-carboxamide

-
37°C, pH 7.4
2.3
4(R)-N-(2-nitroxyethyl)-2-oxothiazolidine-4-carboxamide
-
37°C, pH 7.4
2.3
4(R)-N-(2-nitroxyethyl)-2-oxothiazolidine-4-carboxamide
-
37°C, pH 7.4
9
gonadotropin-releasing hormone

37°C, pH 7.4, native enzyme
12
gonadotropin-releasing hormone
37°C, pH 7.4, recombinant enzyme
2.4
L-2-oxoimidazolidine-4-carboxyl-L-Ala

-
37°C, pH 7.4
2.7
L-2-oxoimidazolidine-4-carboxyl-L-Ala
-
37°C, pH 7.4
3.6
L-2-oxoimidazolidine-4-carboxyl-L-Ala
-
37°C, pH 7.4
6.4
L-2-oxooxazolidine-4-carboxyl-L-Ala

-
37°C, pH 7.4
6.6
L-2-oxooxazolidine-4-carboxyl-L-Ala
-
37°C, pH 7.4
8.1
L-2-oxooxazolidine-4-carboxyl-L-Ala
-
37°C, pH 7.4
2.3
L-2-oxothiazolidine-4-carboxyl-L-Ala

-
37°C, pH 7.4
2.5
L-2-oxothiazolidine-4-carboxyl-L-Ala
-
37°C, pH 7.4
3.4
L-2-oxothiazolidine-4-carboxyl-L-Ala
-
37°C, pH 7.4
47
L-pyroglutamyl-7-amido-4-methylcoumarin

37°C, pH 7.4, recombinant enzyme
58
L-pyroglutamyl-7-amido-4-methylcoumarin
37°C, pH 7.4, native enzyme
0.5
L-pyroglutamyl-beta-naphthylamide

-
pH 7.0, 37°C, mutant enzyme F142A
0.8
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, mutant enzyme F142Y
54.7
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, mutant enzyme F10Y
163
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, mutant enzyme F13Y
210
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, mutant enzyme F142Y
319
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, wild-type enzyme
641
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, mutant enzyme C68S/S185C
770
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, mutant enzyme S185C
785
L-pyroglutamyl-beta-naphthylamide
-
pH 7.0, 37°C, wild-type enzyme
2.4
L-pyroglutamyl-L-Ala

-
37°C, pH 7.4
3.2
L-pyroglutamyl-L-Ala
-
37°C, pH 7.4
3.7
L-pyroglutamyl-L-Ala
-
37°C, pH 7.4
52
L-pyroglutamyl-p-nitroanilide

37°C, pH 7.4, native enzyme
64
L-pyroglutamyl-p-nitroanilide
37°C, pH 7.4, recombinant enzyme
7.4
Pyroglutamyl-beta-naphthylamide

-
mutant enzyme D89N
28
Pyroglutamyl-beta-naphthylamide
-
mutant enzyme E10Q
31.4
Pyroglutamyl-beta-naphthylamide
-
wild-type enzyme
35.1
Pyroglutamyl-beta-naphthylamide
-
mutant enzyme E22Q
20
thyrotrophin-releasing hormone

37°C, pH 7.4, native enzyme
22
thyrotrophin-releasing hormone
37°C, pH 7.4, recombinant enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
102
peak temperature on the differential scanning calorimetry is 101.7°C, pH 9,6, cysteine-free mutant enzyme C142S/C188S
22
-
room temperature, 40% loss of activity after 15 days, 57% loss of activity after 1 month
24
-
50 mM phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 28% loss of activity after 1 month
30
-
pH 7.0, 30 min, about 5% loss of activity of wild-type enzyme and of mutant enzyme S185C
37
-
1 h, rapid inactivation
45
-
stable for at least 1 h
47
-
thermodynamics of heat denaturation of the monomeric enzyme form of mutant enzyme C142S/C188S at pH 2.3. The mechanism of refolding is a two-state process. The equilibrium establishes with a relaxation time of 5080 s at Tm = 46.5°C
55
-
partial inactivation
59
peak temperature on the differential scanning calorimetry is 59.3°C, pH 2.15, cysteine-free mutant enzyme C142S/C188S
79
peak temperature on the differential scanning calorimetry is 78.9°C, pH 3.04 cysteine-free mutant enzyme C142S/C188S
80
-
pH 7.0, 30 min, complete loss of activity of mutant enzyme S185C and mutant enzyme C68S/S185C
40

-
pH 7, 30 min, 5 mM EDTA and 2-mercaptoethanol, stable
40
-
pH 7.0, 30 min, about 30% loss of activity of wild-type enzyme, about 5% loss of activity of mutant enzyme S185C and mutant enzyme C68S/S185C
50

-
pH 7.0, 30 min, about 95% loss of activity of wild-type enzyme, about 10% loss of activity of mutant enzyme S185C and mutant enzyme C68S/S185C
50
-
pH 7.8, 15 min, stable
50
90 min, enzyme is completely stable in crude preparation
60

-
2 min, total inactivation
60
30 min, activity is almost completely lost
70

-
half-life: 1 h
70
half-life: approximately 1 h
75

-
pH 7.0, 30 min, about 65% loss of activity of mutant enzyme S185C and about 80% loss of activity of mutant enzyme C68S/S185C
75
150 min, more than 80% of the activity is retained
89

peak temperature on the differential scanning calorimetry is 77.6°C, pH 7.3, cysteine-free mutant enzyme C142S/C188S
89
peak temperature on the differential scanning calorimetry is 88.8°C, pH 8,7, cysteine-free mutant enzyme C142S/C188S
additional information

-
the heat denaturation of wild-type enzyme and mutant enzymes C188S and C142S/C188S is highly reversible in the dimeric forms, but completely irreversible in the tetrameric form
additional information
the thermal stability of the mutant enzymes C142S/C188S/E192A, C142S/C188S/E192I, C142S/C188S/E192V, C142S/C188S/E192D and C142S/C188S/E192Q at pH 2.15, pH 3.04 and pH 7.3 is less than that of the cysteine-free mutant enzyme C142S/C188S. At pH 8.7 and 9.6 the thermal stability of mutant enzymes C142S/C188S/E192A, C142S/C188S/E192I, C142S/C188S/E192V and C142S/C188S/E192Q is higher than that of the cysteine-free mutant C142S/C188S. The thermal stability of mutant enzyme C142S/C188S/E192D at pH 8.7 and 9.6 is less than that of cysteine-free mutant enzyme C142S/C188S
additional information
-
the thermal stability of the mutant enzymes C142S/C188S/E192A, C142S/C188S/E192I, C142S/C188S/E192V, C142S/C188S/E192D and C142S/C188S/E192Q at pH 2.15, pH 3.04 and pH 7.3 is less than that of the cysteine-free mutant enzyme C142S/C188S. At pH 8.7 and 9.6 the thermal stability of mutant enzymes C142S/C188S/E192A, C142S/C188S/E192I, C142S/C188S/E192V and C142S/C188S/E192Q is higher than that of the cysteine-free mutant C142S/C188S. The thermal stability of mutant enzyme C142S/C188S/E192D at pH 8.7 and 9.6 is less than that of cysteine-free mutant enzyme C142S/C188S
additional information
alpha6-helix (from Ser188 to Glu205) of PCP-0SH is stably formed in the D1 state
additional information
-
alpha6-helix (from Ser188 to Glu205) of PCP-0SH is stably formed in the D1 state
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.