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Literature summary for 3.4.19.3 extracted from
- Purification and characterization of recombinant pyrrolidone carboxyl peptidase of Bacillus subtilis (1992), J. Chromatogr., 584, 101-107.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | application to N-terminal pyroglutamyl unblocking prior to Edman sequential degradation in peptide and protein sequencing | Bacillus subtilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.04 | - |
Pyroglutamyl-beta-naphthylamide | - |
Bacillus subtilis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25200 | - |
4 * 25200, SDS-PAGE | Bacillus subtilis |
| 91000 | - |
non-denaturing PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.2 | - |
- |
Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 70% loss of activity after 1 month | Bacillus subtilis |
| 4°C, 50 mM phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 13% loss of activity after 1 month | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyroglutamyl-beta-naphthylamide + H2O | - |
Bacillus subtilis | pyroglutamate + beta-naphthylamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 25200, SDS-PAGE | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | - |
50 mM phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 28% loss of activity after 1 month | Bacillus subtilis |
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