Literature summary for 3.4.19.3 extracted from

Schaeffer, M.; de Miranda, A.; Mottram, J.C.; Coombs, G.H.
Differentiation of Leishmania major is impaired by over-expression of pyroglutamyl peptidase I (2006), Mol. Biochem. Parasitol., 150, 318-329.

Search for more literature for 3.4.19.3

Activating Compound

Activating Compound	Comment	Organism	Structure
dithiothreitol	at least 10 mM required for full activity	Leishmania major
tris(2-carboxyethyl)phosphine	at least 0.5 mM required for full activity	Leishmania major

Application

Application	Comment	Organism
medicine	overexpression of active PPI in Leishmania major impairs differentiation from the procyclic promastigote to the infective metacyclic promastigote. PPI does not protect against the natural antimicrobial peptide gomesin	Leishmania major

Cloned(Commentary)

Cloned (Comment)	Organism
N-terminal His-tagged native or mutant PPI expressed in Escherichia coli BL21(DE3)	Leishmania major

Protein Variants

Protein Variants	Comment	Organism
C210A	no detectable activity	Leishmania major
E101Q	ca. 2% activity of the native enzyme	Leishmania major
E107Q	ca. 50% activity of the native enzyme	Leishmania major
H234S	no detectable activity	Leishmania major
additional information	PPI-deficient mutants show no detectable phenotype, retain infectivity to macrophages in vitro and in mice	Leishmania major

Inhibitors

Inhibitors	Comment	Organism	Structure
2-Pyrrolidinone	1 mM inhibits by 44%	Leishmania major
Cu2+	1 mM inhibits by 91.3%	Leishmania major
iodoacetamide	0.1 mM strongly inhibits by 98.3%	Leishmania major
iodoacetic acid	0.1 mM completely inhibits	Leishmania major
additional information	Ca2+, Mg2+, Na+, Li+, pepstatin A, 1,10-phenanthroline, benzamidine, bestatin and trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane have no or little inhibitory effect	Leishmania major
N-ethylmaleimide	0.1 mM strongly inhibits by 98.1%	Leishmania major
Ni2+	1 mM inhibits by 99%, 0.1 mM inhibits by 48%	Leishmania major
phenylmethylsulfonyl fluoride	1 mM inhibits by 53.4%	Leishmania major
Zn2+	1 mM inhibits by 80.2%	Leishmania major

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0335	-	L-pyroglutamyl p-nitroanilide	-	Leishmania major

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Leishmania major	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30270	-	sequence analysis	Leishmania major
36000	-	gel filtration	Leishmania major

Organism

Organism	UniProt	Comment	Textmining
Leishmania major	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by Ni-affinity chromatography and gel filtration	Leishmania major

Source Tissue

Source Tissue	Comment	Organism	Textmining
promastigote	-	Leishmania major	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-pyroglutamyl-beta-naphthylamide + H2O	-	Leishmania major	L-pyroglutamate + 2-naphtylamine	-	?
L-pyroglutamyl-p-nitroanilide + H2O	-	Leishmania major	L-pyroglutamate + p-nitroaniline	-	?

Synonyms

Synonyms	Comment	Organism
PPI	-	Leishmania major
pyroglutamyl aminopeptidase I	-	Leishmania major

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	optimal activity between 8.8-10.5, activity decreases rapidly below pH 8.0	Leishmania major

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Release 2023.1 (January 2023)