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Literature summary for 3.4.19.3 extracted from
- Pyroglutamyl peptidase type I from Trypanosoma brucei: a new virulence factor from African trypanosomes that de-blocks regulatory peptides in the plasma of infected hosts (2006), Biochem. J., 394, 635-645.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Trypanosoma brucei brucei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Pyrrolidone | - |
Trypanosoma brucei brucei |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
L-pyroglutamyl-7-amido-4-methylcoumarin | 37°C, pH 7.4, native enzyme | Trypanosoma brucei brucei | |
| 0.028 | - |
L-pyroglutamyl-p-nitroanilide | 37°C, pH 7.4, native enzyme | Trypanosoma brucei brucei | |
| 0.028 | - |
L-pyroglutamyl-7-amido-4-methylcoumarin | 37°C, pH 7.4, recombinant enzyme | Trypanosoma brucei brucei | |
| 0.032 | - |
thyrotrophin-releasing hormone | 37°C, pH 7.4, recombinant enzyme | Trypanosoma brucei brucei | |
| 0.041 | - |
thyrotrophin-releasing hormone | 37°C, pH 7.4, native enzyme | Trypanosoma brucei brucei | |
| 0.044 | - |
L-pyroglutamyl-p-nitroanilide | 37°C, pH 7.4, recombinant enzyme | Trypanosoma brucei brucei | |
| 0.079 | - |
gonadotropin-releasing hormone | 37°C, pH 7.4, native enzyme | Trypanosoma brucei brucei | |
| 0.089 | - |
gonadotropin-releasing hormone | 37°C, pH 7.4, recombinant enzyme | Trypanosoma brucei brucei | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thyrotrophin-releasing hormone + H2O | Trypanosoma brucei brucei | trypanosomal pyroglutamyl aminopeptidase I is responsible for the abnormally rapid degradation of thyrotrophin-releasing hormone in the circulation of infected rats | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
- |
- |
| Trypanosoma brucei brucei | Q4U338 | - |
- |
| Trypanosoma brucei gambiense | - |
- |
- |
| Trypanosoma brucei rhodesiense | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from Trypanosoma brucei-infected rat plasma | Trypanosoma brucei brucei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bloodstream form | - |
Trypanosoma brucei | - |
| bloodstream form | - |
Trypanosoma brucei rhodesiense | - |
| bloodstream form | - |
Trypanosoma brucei gambiense | - |
| trypanosomoid form | - |
Trypanosoma brucei brucei | - |
| trypomastigote | - |
Trypanosoma brucei brucei | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Trypanosoma brucei brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gonadotropin-releasing hormone + H2O | hydrolyzed exclusively at the pyroglutamyl-/-Xaa bond | Trypanosoma brucei brucei | ? | - |
? | |
| L-pyroglutamyl-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma brucei brucei | L-pyroglutamate + 7-amino-4-methylcoumarin | - |
? | |
| L-pyroglutamyl-p-nitroanilide + H2O | - |
Trypanosoma brucei brucei | L-pyroglutamate + p-nitroaniline | - |
? | |
| thyrotrophin-releasing hormone + H2O | trypanosomal pyroglutamyl aminopeptidase I is responsible for the abnormally rapid degradation of thyrotrophin-releasing hormone in the circulation of infected rats | Trypanosoma brucei brucei | ? | - |
? | |
| thyrotrophin-releasing hormone + H2O | hydrolyzed exclusively at the pyroglutamyl-/-Xaa bond | Trypanosoma brucei brucei | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | stable | Trypanosoma brucei brucei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9 | - |
gonadotropin-releasing hormone | 37°C, pH 7.4, native enzyme | Trypanosoma brucei brucei | |
| 12 | - |
gonadotropin-releasing hormone | 37°C, pH 7.4, recombinant enzyme | Trypanosoma brucei brucei | |
| 20 | - |
thyrotrophin-releasing hormone | 37°C, pH 7.4, native enzyme | Trypanosoma brucei brucei | |
| 22 | - |
thyrotrophin-releasing hormone | 37°C, pH 7.4, recombinant enzyme | Trypanosoma brucei brucei | |
| 47 | - |
L-pyroglutamyl-7-amido-4-methylcoumarin | 37°C, pH 7.4, recombinant enzyme | Trypanosoma brucei brucei | |
| 52 | - |
L-pyroglutamyl-p-nitroanilide | 37°C, pH 7.4, native enzyme | Trypanosoma brucei brucei | |
| 58 | - |
L-pyroglutamyl-7-amido-4-methylcoumarin | 37°C, pH 7.4, native enzyme | Trypanosoma brucei brucei | |
| 64 | - |
L-pyroglutamyl-p-nitroanilide | 37°C, pH 7.4, recombinant enzyme | Trypanosoma brucei brucei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Trypanosoma brucei brucei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH 6.0: about 30% of maximal activity, pH 9.0: about 40% of maximal activity | Trypanosoma brucei brucei |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.062 | - |
2-Pyrrolidone | - |
Trypanosoma brucei brucei | |
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