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Information on EC 3.1.2.20 - acyl-CoA hydrolase
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EC Tree
3.1.2.20 acyl-CoA hydrolaseIUBMB Comments
Broad specificity for medium- to long-chain acyl-CoA. Insensitive to NAD+ (cf. EC 3.1.2.19 ADP-dependent medium-chain-acyl-CoA hydrolase)
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Word Map
- 3.1.2.20
- acyl-coas
- palmitoyl-coa
-
3.1.2.2
-
acyl-coa:cholesterol
- coash
- oleoyl-coa
-
teiis
-
hotdog-fold
-
acots
- arachidonoyl-coa
- degradation
- decanoyl-coa
- biotechnology
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
acyl-coa hydrolase, them2, type ii thioesterase, them1, acot11, them4, acot13, thioesterase superfamily member 2, te ii, thioesterase 2, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ACH1
-
-
-
-
ACH2
-
-
-
-
Acot7
ACT
-
-
-
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acyl CoA hydrolase
-
-
-
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acyl coenzyme A hydrolase
-
-
-
-
acyl coenzyme A thioesterase
-
-
-
-
acyl-CoA hydrolase
acyl-CoA thioesterase
brain acyl-CoA hydrolase
CalG
EC 3.1.2.2
-
-
related
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fatty acyl thioesterase I
-
-
-
-
FscTE
HIV-Nef associated acyl coA thioesterase
-
-
-
-
hTHEM2
hydrolase, acyl coenzyme A
-
-
-
-
hydrolase, palmitoyl coenzyme A
-
-
-
-
LACH1
-
-
-
-
LACH2
-
-
-
-
long chain acyl-CoA hydrolase
-
-
-
-
long chain acyl-CoA thioesterase
-
-
-
-
long chain fatty-acyl-CoA hydrolase
-
-
-
-
long chain fatty-acyl-CoA thioesterase
-
-
-
-
long-chain acyl-CoA hydrolase
mitochondrial acyl-CoA thioesterase
-
-
-
-
palmitoyl coenzyme A hydrolase
-
-
-
-
palmitoyl thioesterase
-
-
-
-
palmitoyl-CoA deacylase
-
-
-
-
palmitoyl-CoA hydrolase
palmityl coenzyme A deacylase
-
-
-
-
palmityl thioesterase
-
-
-
-
palmityl thioesterase I
-
-
-
-
palmityl thioesterase II
-
-
-
-
TE
-
-
-
-
TE-II
-
-
-
-
TEII
TEII associated with the FR-008/candicidin gene cluster
Them5
thioesterase
thioesterase B
-
-
-
-
thioesterase II
-
-
-
-
type II thioesterase
very long chain acyl-CoA thioesterase
-
-
-
-
YciA
ZAP128
-
-
-
-
additional information
acyl-CoA thioesterase
-
-
-
-
brain acyl-CoA hydrolase
-
-
-
-
palmitoyl-CoA hydrolase
-
-
-
-
additional information
-
immunologically not related with the thioesterase of fatty acid synthase or the acyl fatty acid synthase thioester hydrolase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
an acyl-CoA + H2O = CoA + a carboxylate
catalytic triad D337-Q409-S359
-
an acyl-CoA + H2O = CoA + a carboxylate
based on the results of quantum mechanics/molecular mechanics metadynamics techniques simulations, it is demonstrated that hotdog-fold hTHEM2 follows a simple mechanism in which a water molecule directly attacks the carbonyl carbon atom to cleave the thioester bond without requiring an enzyme-substrate covalent intermediate the reaction, providing evidence for the formation of a tetrahedral-like transition state
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an acyl-CoA + H2O = CoA + a carboxylate
molecular dynamics studies show that certain residues of the substrate binding tunnel are flexibel and thus modulate the length of the tunnel
an acyl-CoA + H2O = CoA + a carboxylate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of thioester
hydrolysis of thioester
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
acyl-CoA hydrolase
Broad specificity for medium- to long-chain acyl-CoA. Insensitive to NAD+ (cf. EC 3.1.2.19 ADP-dependent medium-chain-acyl-CoA hydrolase)
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CAS REGISTRY NUMBER
COMMENTARY
37270-64-7
EC 3.1.2.2
9025-87-0
EC 3.1.2.20
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-nitrophenyl acetate + H2O
2-nitrophenol + acetate
synthetic substrate
-
-
?
3,4-dihydroxyphenylacetyl-CoA + H2O
CoA + 3,4-dihydroxyphenylacetate
-
-
-
?
3,5-cis-dodecadienoyl-CoA + H2O
CoA + 3,5-cis-dodecadienoic acid
-
-
-
?
3,5-cis-tetradecadienoyl-CoA + H2O
CoA + 3,5-cis-tetradecadienoic acid
-
-
-
?
3,5-dihydroxyphenylacetyl-CoA + H2O
CoA + 3,5-dihydroxyphenylacetate
-
-
-
?
3-hydroxy-3-methyl glutaryl-CoA + H2O
CoA + 3-hydroxy-3-methyl glutarate
-
-
-
?
3-hydroxybutyryl-CoA + H2O
CoA + 3-hydroxybutanoate
-
reaction at 4.3% the rate of acetoacetyl-CoA hydrolysis
-
-
?
3-hydroxytetradecanoyl-CoA + H2O
CoA + 3-hydroxytetradecanoate
-
-
-
?
butanoyl-CoA + H2O
butanoate + CoA
-
highest specific activity of the substrates tested
-
-
?
butyryl-CoA + H2O
CoA + butyrate
-
24% activity compared to cezomycin-CoA
-
-
?
butyryl-[acyl-carrier protein] + H2O
butanoate + acyl-carrier protein
-
-
-
?
butyrylpanthetheine phosphate + H2O
pantetheine phosphate + butyrate
-
-
-
?
N-acetylcysteamine propionate
N-acetylcysteamine + propionate
-
-
-
-
?
nafamostat + H2O
6-hydroxynaphthalene-2-carboximidamide + 4-carbamidamidobenzoic acid
-
-
-
-
?
p-nitrophenyl butanoate + H2O
p-nitrophenol + butanoate
-
51% of the activity with p-nitrophenyl propanoate
-
-
?
p-nitrophenyl dodecanoate + H2O
p-nitrophenol + dodecanoate
-
8.7% of the activity with p-nitrophenyl propanoate
-
-
?
p-nitrophenyl hexanoate + H2O
p-nitrophenol + hexanoate
-
33% of the activity with p-nitrophenyl propanoate
-
-
?
propionyl-[acyl-carrier protein] + H2O
propionate + acyl-carrier protein
-
-
-
?
tetradecylthioacetyl-CoA + H2O
CoA + tetradecylthioacetate
-
best substrate
-
-
?
3-hydroxy-3-methylglutaryl-CoA + H2O
CoA + 3-hydroxy-3-methylglutarate
-
-
-
?
3-hydroxy-3-methylglutaryl-CoA + H2O
CoA + 3-hydroxy-3-methylglutarate
-
-
-
-
?
3-hydroxydecanoyl-CoA + H2O
CoA + 3-hydroxydecanoate
-
reaction at 1.4% the rate of acetoacetyl-CoA hydrolysis
-
-
?
3-hydroxyphenylacetyl-CoA + H2O
CoA + 3-hydroxyphenylacetate
-
-
-
?
4,8-dimethylnonanoyl-CoA + H2O
CoA + 4,8-dimethylnonanoate
-
-
?
4,8-dimethylnonanoyl-CoA + H2O
CoA + 4,8-dimethylnonanoate
involved in pristanic acid beta-oxidation, scheme of the pathway
-
?
4,8-dimethylnonanoyl-CoA + H2O
CoA + 4,8-dimethylnonanoate
hydrolysis allows transport of 4,8-dimethylnonanoate from peroxisomes to mitochondria for full oxidation
-
?
4-hydroxyphenylacetyl-CoA + H2O
CoA + 4-hydroxyphenylacetate
-
-
-
?
acetoacetyl-CoA + H2O
CoA + acetoacetate
-
8% activity compared to palmitoyl-CoA
-
?