Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
3,5-cis-tetradecadienoyl-CoA | - |
Escherichia coli | |
0.0058 | - |
3-hydroxytetradecanoyl-CoA | - |
Escherichia coli | |
0.006 | - |
palmitoyl-CoA | - |
Escherichia coli | |
0.0063 | - |
myristoyl-CoA | - |
Escherichia coli | |
0.0108 | - |
3,5-cis-dodecadienoyl-CoA | - |
Escherichia coli | |
0.013 | - |
lauroyl-CoA | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
4 * 15000, crystal structure analysis, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P77712 | - |
- |
Purification (Comment) | Organism |
---|---|
ion exchange chromatography (DEAE-cellulose), hydroxylapatite chromatography, hydrophobic interaction chromatography, recombinant protein: immobilized metal ion affinity chromatography (Ni2+), ion exchange chromatography (DEAE-cellulose), hydrophobic interaction chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
also comparable activities observed with stearoyl-CoA and oleoyl-CoA | Escherichia coli |
6.9 | - |
with lauroyl-CoA | Escherichia coli |
26.4 | - |
with myristoyl-CoA | Escherichia coli |
43.5 | - |
with palmitoyl-CoA | Escherichia coli |
45.5 | - |
with 3-hydroxytetradecanoyl-CoA | Escherichia coli |
92.3 | - |
with 3,5-cis-dodecadienoyl-CoA | Escherichia coli |
101.3 | - |
with 3,5-cis-tetradecadienoyl-CoA | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,5-cis-dodecadienoyl-CoA + H2O | - |
Escherichia coli | CoA + 3,5-cis-dodecadienoic acid | - |
? | |
3,5-cis-tetradecadienoyl-CoA + H2O | - |
Escherichia coli | CoA + 3,5-cis-tetradecadienoic acid | - |
? | |
3-hydroxytetradecanoyl-CoA + H2O | - |
Escherichia coli | CoA + 3-hydroxytetradecanoate | - |
? | |
lauroyl-CoA + H2O | - |
Escherichia coli | CoA + laurate | - |
? | |
myristoyl-CoA + H2O | - |
Escherichia coli | CoA + myristate | - |
? | |
palmitoyl-CoA + H2O | - |
Escherichia coli | CoA + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 15000, crystal structure analysis, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
thioesterase III | - |
Escherichia coli |
ybaW protein | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inactive with substrates having acyl chains with fewer than 12 carbon atoms | Escherichia coli | |
1.6 | - |
lauroyl-CoA | - |
Escherichia coli | |
2 | 3.7 | 3,5-cis-tetradecadienoyl-CoA | - |
Escherichia coli | |
6.2 | - |
myristoyl-CoA | - |
Escherichia coli | |
10.2 | - |
palmitoyl-CoA | - |
Escherichia coli | |
10.7 | - |
3-hydroxytetradecanoyl-CoA | - |
Escherichia coli | |
21.6 | - |
3,5-cis-dodecadienoyl-CoA | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
100 | - |
lauroyl-CoA | - |
Escherichia coli | |
1000 | - |
myristoyl-CoA | - |
Escherichia coli | |
1700 | - |
palmitoyl-CoA | - |
Escherichia coli | |
1800 | - |
3-hydroxytetradecanoyl-CoA | - |
Escherichia coli | |
2000 | - |
3,5-cis-dodecadienoyl-CoA | - |
Escherichia coli | |
7900 | - |
3,5-cis-tetradecadienoyl-CoA | - |
Escherichia coli |