Literature summary for 3.1.2.20 extracted from

De Marcos Lousa, C.; van Roermund, C.W.; Postis, V.L.; Dietrich, D.; Kerr, I.D.; Wanders, R.J.; Baldwin, S.A.; Baker, A.; Theodoulou, F.L.
Intrinsic acyl-CoA thioesterase activity of a peroxisomal ATP binding cassette transporter is required for transport and metabolism of fatty acids (2013), Proc. Natl. Acad. Sci. USA, 110, 1279-1284.

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Activating Compound

Activating Compound	Comment	Organism	Structure
ATP	-	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
recombinantly expressed in insect cells	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
S810N	mutant is defective in fatty acid degradation in vivo, retains ATPase activity and has strongly reduced thioesterase activity	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0015	-	stearoyl-CoA	pH 8, temperature not specified in the publication	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Arabidopsis thaliana	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
150000	-	SDS-PAGE	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
myristoyl-CoA + H2O	lower activity with myristoyl-CoA compared to stearoyl-CoA	Arabidopsis thaliana	CoA + myristate	-	?
stearoyl-CoA + H2O	-	Arabidopsis thaliana	CoA + stearate	-	?

Synonyms

Synonyms	Comment	Organism
acyl-CoA thioesterase	-	Arabidopsis thaliana
comatose	-	Arabidopsis thaliana
CTS	-	Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5.7	-	stearoyl-CoA	pH 8, temperature not specified in the publication	Arabidopsis thaliana

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Release 2023.1 (January 2023)