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Literature summary for 3.1.2.20 extracted from

  • Kim, B.S.; Cropp, T.A.; Beck, B.J.; Sherman, D.H.; Reynolds, K.A.
    Biochemical evidence for an editing role of thioesterase II in the biosynthesis of the polyketide pikromycin (2002), J. Biol. Chem., 277, 48028-48034.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 as His-tagged protein Streptomyces venezuelae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptomyces venezuelae involved in the biosynthesis of the polyketide pikromycin ?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces venezuelae Q9ZGI1 thioesterase II, gene pikAV
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli BL21 as His-tagged enzyme, to homogeneity Streptomyces venezuelae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O poor substrate Streptomyces venezuelae CoA + acetate
-
?
acyl-CoA + H2O
-
Streptomyces venezuelae CoA + a carboxylate
-
?
butyryl-[acyl-carrier protein] + H2O
-
Streptomyces venezuelae butanoate + acyl-carrier protein
-
?
malonyl-CoA + H2O
-
Streptomyces venezuelae CoA + malonate
-
?
additional information involved in the biosynthesis of the polyketide pikromycin Streptomyces venezuelae ?
-
?
n-butyryl-CoA + H2O
-
Streptomyces venezuelae CoA + n-butanoate
-
?
propionyl-CoA + H2O
-
Streptomyces venezuelae CoA + propionate
-
?
propionyl-[acyl-carrier protein] + H2O
-
Streptomyces venezuelae propionate + acyl-carrier protein
-
?