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Literature summary for 3.1.2.20 extracted from

  • Tilton, G.; Shockey, J.; Browse, J.
    Two families of acyl-CoA thioesterases in Arabidopsis (2000), Biochem. Soc. Trans., 28, 946-947.
    View publication on PubMed
Search for more literature for 3.1.2.20

Application

Application Comment Organism
biotechnology enzyme is involved in fatty acid biosynthesis and may be a good target for improvement of special oil production in transgenic plants Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of isozymes AtACH5and AtACH2 in Escherichia coli , AtACH2 fused to maltose-binding protein Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information a AtACH5 gene knock-out mutant shows no altered phenotype Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum isozymes AtACH4 and AtACH5 Arabidopsis thaliana 5783
-
peroxisome isozymes AtACH1 and AtACH2 Arabidopsis thaliana 5777
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + H2O Arabidopsis thaliana
-
 CoA + a carboxylate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 2 families of acyl-CoA thioesterases due to their localization: 1. isozymes ACH1 and ACH2, 2. isozymes ACH4 and ACH5
-

Purification (Commentary)

Purification (Comment) Organism
partially, recombinant isozyme AtACH2 fused to maltose-binding protein from Escherichia coli Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + H2O
-
 Arabidopsis thaliana CoA + a carboxylate
-
 ?
hexadecanoyl-CoA + H2O
-
 Arabidopsis thaliana CoA + hexadecanoate
-
 ?
oleoyl-CoA + H2O
-
 Arabidopsis thaliana CoA + oleate
-
 ?