Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activation | Euglena gracilis | |
bovine serum albumin | effect of stimulation increases as chain-length increases up to C-12 and decreases progressively as chain-length increases beyond C-12, inhibitory with octadecanoyl-CoA as substrate | Euglena gracilis | |
hexadecanol | activation, only with acyl-CoA of more than C-8 as substrates | Euglena gracilis | |
Tetradecanol | activation, only with acyl-CoA of more than C-8 as substrates | Euglena gracilis | |
Triton X-100 | activation, 0.005%, dodecanoyl-CoA as substrate | Euglena gracilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | octadecanoyl-CoA as substrate, stimulates with C-6 to C-12 CoA as substrates | Euglena gracilis | |
diethyldicarbonate | hydroxylamine reverses | Euglena gracilis | |
diisopropylfluorophosphate | - |
Euglena gracilis | |
dodecanoyl-CoA | substrate inhibition | Euglena gracilis | |
additional information | no inhibition by NEM | Euglena gracilis | |
O,O-Diethyl-O-(2,4,5-trichlorophenyl)-phosphate | - |
Euglena gracilis | |
O,O-Diethyl-O-(3,5,6-trichloro-2-pyridyl)-phosphate | - |
Euglena gracilis | |
O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl)-phosphorothionate | i.e. Chloropyrifos | Euglena gracilis | |
O,O-Dimethyl-O-(2,4,5-trichloro-1-phenyl)-phosphate | - |
Euglena gracilis | |
O,O-Dimethyl-O-(3,5,6-trichloro-2-pyridyl)-phosphate | i.e. Fospirate | Euglena gracilis | |
p-hydroxymercuribenzoate | weak inhibition | Euglena gracilis | |
tetradecanoyl-CoA | substrate inhibition | Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effect of pH on kinetic data | Euglena gracilis | |
0.014 | - |
dodecanoyl-CoA | pH 7.0, 30°C | Euglena gracilis | |
0.017 | - |
hexadecanoyl-CoA | pH 7.0, 30°C | Euglena gracilis | |
0.024 | - |
tetradecanoyl-CoA | pH 7.0, 30°C | Euglena gracilis | |
0.027 | - |
decanoyl-CoA | pH 7.0, 30°C | Euglena gracilis | |
0.034 | - |
octadecanoyl-CoA | pH 7.0, 30°C | Euglena gracilis | |
0.043 | - |
octanoyl-CoA | pH 7.0, 30°C | Euglena gracilis | |
0.077 | - |
hexanoyl-CoA | pH 7.0, 30°C | Euglena gracilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Euglena gracilis | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
gel filtration | Euglena gracilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + H2O | Euglena gracilis | - |
CoA + a carboxylate | - |
? | |
hexadecanoyl-CoA + H2O | Euglena gracilis | - |
CoA + hexadecanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
Z, dark-grown | - |
Euglena gracilis Z | - |
Z, dark-grown | - |
Purification (Comment) | Organism |
---|---|
678fold | Euglena gracilis |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.5 | - |
purified enzyme, lauroyl-CoA as substrate | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + H2O | - |
Euglena gracilis | CoA + a carboxylate | - |
? | |
acyl-CoA + H2O | broad specificity for medium-chain to long-chain acyl-CoA | Euglena gracilis | CoA + a carboxylate | - |
? | |
decanoyl-CoA + H2O | - |
Euglena gracilis | CoA + decanoate | - |
? | |
dodecanoyl-CoA + H2O | i.e. lauroyl-CoA | Euglena gracilis | CoA + dodecanoate | - |
? | |
hexadecanoyl-CoA + H2O | - |
Euglena gracilis | CoA + hexadecanoate | - |
? | |
hexanoyl-CoA + H2O | poor substrate | Euglena gracilis | CoA + hexanoate | - |
? | |
n-butyryl-CoA + H2O | poor substrate | Euglena gracilis | CoA + n-butanoate | - |
? | |
octadecanoyl-CoA + H2O | preferred substrate | Euglena gracilis | CoA + octadecanoate | - |
? | |
octadecanoyl-CoA + H2O | i.e. stearoyl-CoA | Euglena gracilis | CoA + octadecanoate | - |
? | |
octanoyl-CoA + H2O | - |
Euglena gracilis | CoA + octanoate | - |
? | |
tetradecanoyl-CoA + H2O | best substrate | Euglena gracilis | CoA + tetradecanoate | - |
? | |
tetradecanoyl-CoA + H2O | i.e. myristoyl-CoA | Euglena gracilis | CoA + tetradecanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Euglena gracilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Euglena gracilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-optimum depends on chain-length of acyl-CoA substrate | Euglena gracilis |
4.5 | - |
hexanoyl-CoA or octanoyl-CoA as substrate | Euglena gracilis |
5 | - |
decanoyl-CoA as substrate | Euglena gracilis |
5.5 | - |
dodecanoyl-CoA as substrate | Euglena gracilis |
6 | 6.5 | tetradecanoyl-CoA as substrate | Euglena gracilis |
6.5 | - |
hexadecanoyl-CoA as substrate | Euglena gracilis |
7 | - |
octadecanoyl-CoA as substrate | Euglena gracilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | about 75% of maximal activity at pH 4.5 and about half-maximal activity at pH 7.0, dodecanoyl-CoA as substrate | Euglena gracilis |
6.2 | 8 | about half-maximal activity at pH 6.2 and pH 8.0, octadecanoyl-CoA as substrate | Euglena gracilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme is unstable to low pH | Euglena gracilis |