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Literature summary for 3.1.2.20 extracted from

  • Larson, J.D.; Kolattukudy, P.E.
    Isolation and characterization of an acyl-CoA thioesterase from dark-grown Euglena gracilis (1985), Arch. Biochem. Biophys., 237, 27-37.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation Euglena gracilis
bovine serum albumin effect of stimulation increases as chain-length increases up to C-12 and decreases progressively as chain-length increases beyond C-12, inhibitory with octadecanoyl-CoA as substrate Euglena gracilis
hexadecanol activation, only with acyl-CoA of more than C-8 as substrates Euglena gracilis
Tetradecanol activation, only with acyl-CoA of more than C-8 as substrates Euglena gracilis
Triton X-100 activation, 0.005%, dodecanoyl-CoA as substrate Euglena gracilis

Inhibitors

Inhibitors Comment Organism Structure
bovine serum albumin octadecanoyl-CoA as substrate, stimulates with C-6 to C-12 CoA as substrates Euglena gracilis
diethyldicarbonate hydroxylamine reverses Euglena gracilis
diisopropylfluorophosphate
-
Euglena gracilis
dodecanoyl-CoA substrate inhibition Euglena gracilis
additional information no inhibition by NEM Euglena gracilis
O,O-Diethyl-O-(2,4,5-trichlorophenyl)-phosphate
-
Euglena gracilis
O,O-Diethyl-O-(3,5,6-trichloro-2-pyridyl)-phosphate
-
Euglena gracilis
O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl)-phosphorothionate i.e. Chloropyrifos Euglena gracilis
O,O-Dimethyl-O-(2,4,5-trichloro-1-phenyl)-phosphate
-
Euglena gracilis
O,O-Dimethyl-O-(3,5,6-trichloro-2-pyridyl)-phosphate i.e. Fospirate Euglena gracilis
p-hydroxymercuribenzoate weak inhibition Euglena gracilis
tetradecanoyl-CoA substrate inhibition Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information effect of pH on kinetic data Euglena gracilis
0.014
-
dodecanoyl-CoA pH 7.0, 30°C Euglena gracilis
0.017
-
hexadecanoyl-CoA pH 7.0, 30°C Euglena gracilis
0.024
-
tetradecanoyl-CoA pH 7.0, 30°C Euglena gracilis
0.027
-
decanoyl-CoA pH 7.0, 30°C Euglena gracilis
0.034
-
octadecanoyl-CoA pH 7.0, 30°C Euglena gracilis
0.043
-
octanoyl-CoA pH 7.0, 30°C Euglena gracilis
0.077
-
hexanoyl-CoA pH 7.0, 30°C Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Euglena gracilis 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + H2O Euglena gracilis
-
CoA + a carboxylate
-
?
hexadecanoyl-CoA + H2O Euglena gracilis
-
CoA + hexadecanoate
-
?

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
Z, dark-grown
-
Euglena gracilis Z
-
Z, dark-grown
-

Purification (Commentary)

Purification (Comment) Organism
678fold Euglena gracilis

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.5
-
purified enzyme, lauroyl-CoA as substrate Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + H2O
-
Euglena gracilis CoA + a carboxylate
-
?
acyl-CoA + H2O broad specificity for medium-chain to long-chain acyl-CoA Euglena gracilis CoA + a carboxylate
-
?
decanoyl-CoA + H2O
-
Euglena gracilis CoA + decanoate
-
?
dodecanoyl-CoA + H2O i.e. lauroyl-CoA Euglena gracilis CoA + dodecanoate
-
?
hexadecanoyl-CoA + H2O
-
Euglena gracilis CoA + hexadecanoate
-
?
hexanoyl-CoA + H2O poor substrate Euglena gracilis CoA + hexanoate
-
?
n-butyryl-CoA + H2O poor substrate Euglena gracilis CoA + n-butanoate
-
?
octadecanoyl-CoA + H2O preferred substrate Euglena gracilis CoA + octadecanoate
-
?
octadecanoyl-CoA + H2O i.e. stearoyl-CoA Euglena gracilis CoA + octadecanoate
-
?
octanoyl-CoA + H2O
-
Euglena gracilis CoA + octanoate
-
?
tetradecanoyl-CoA + H2O best substrate Euglena gracilis CoA + tetradecanoate
-
?
tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Euglena gracilis CoA + tetradecanoate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE Euglena gracilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Euglena gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pH-optimum depends on chain-length of acyl-CoA substrate Euglena gracilis
4.5
-
hexanoyl-CoA or octanoyl-CoA as substrate Euglena gracilis
5
-
decanoyl-CoA as substrate Euglena gracilis
5.5
-
dodecanoyl-CoA as substrate Euglena gracilis
6 6.5 tetradecanoyl-CoA as substrate Euglena gracilis
6.5
-
hexadecanoyl-CoA as substrate Euglena gracilis
7
-
octadecanoyl-CoA as substrate Euglena gracilis

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 about 75% of maximal activity at pH 4.5 and about half-maximal activity at pH 7.0, dodecanoyl-CoA as substrate Euglena gracilis
6.2 8 about half-maximal activity at pH 6.2 and pH 8.0, octadecanoyl-CoA as substrate Euglena gracilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
enzyme is unstable to low pH Euglena gracilis