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Information on EC 2.4.1.65 - 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase and Organism(s) Homo sapiens and UniProt Accession P21217

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IUBMB Comments
This enzyme is the product of the Lewis blood group gene. Normally acts on a glycoconjugate where R (see reaction) is a glycoprotein or glycolipid. Although it is a 4-fucosyltransferase, it has a persistent 3-fucosyltransferase activity towards the glucose residue in free lactose. This enzyme fucosylates on O-4 of an N-acetylglucosamine that carries a galactosyl group on O-3, unlike EC 2.4.1.152, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, which fucosylates on O-3 of an N-acetylglucosamine that carries a galactosyl group on O-4. Enzymes catalysing the 4-alpha-fucosylation of the GlcNAc in beta-D-Gal-(1->3)-beta-GlcNAc sequences (with some activity also as 3-alpha-fucosyltransferases) are present in plants, where the function in vivo is the modification of N-glycans. In addition, the fucTa gene of Helicobacter strain UA948 encodes a fucosyltransferase with both 3-alpha- and 4-alpha-fucosyltransferase activities.
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Select one or more organisms in this record:
This record set is specific for:
Homo sapiens
UNIPROT: P21217
Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The taxonomic range for the selected organisms is: Homo sapiens
Synonyms
(Lea)-dependent alpha-3/4-fucosyltransferase, alpha 1,3-fucosyltransferase VII, alpha 1,3/4 fucosyltransferase, alpha(1,3) fucosyltransferase, alpha(1,3/1,4) fucosyltransferase III, alpha(1,3/4) fucosyltransferase, alpha(1,3/4)-FUT, alpha-(1,3/1,4)-fucosyltransferase, alpha-(1,3/1,4)-FucT, alpha-(1-3/4)-fucosyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(Lea)-dependent alpha-3/4-fucosyltransferase
-
-
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alpha 1,3-fucosyltransferase VII
247
-
alpha(1,3/1,4) fucosyltransferase III
-
-
-
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alpha(1,3/4) fucosyltransferase
-
-
-
-
alpha-(1-3/4)-fucosyltransferase
247
-
alpha-(1->4)-L-fucosyltransferase
-
-
-
-
alpha-1,3 fucosyltransferase
247
-
alpha-1,3-fucosyltransferase
247
-
alpha-1,3/4-fucosyltransferase
247
-
alpha-1,3fucosyltransferase
247
-
alpha-3-FUT
268842
-
alpha-3/4 fucosyltransferase
247
-
alpha-3/4 fucosyltransferase III
247
-
alpha-3/4-FUT
267765, 268839
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alpha-4-L-fucosyltransferase
-
-
-
-
alpha1,3 fucosyltransferase
247
-
alpha1,3-fucosyltransferase
281755, 290498
-
alpha1,3-fucosyltransferase IX
247
-
alpha1,3-fucosyltransferase VI
247
-
alpha1,3/1,4FucT III
247
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alpha1,3/4-fucosyltransferase
247
-
alpha1,3/4-fucosyltransferase VI
247
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alpha1,3/4-fucosyltransferases-V and -VI
247
-
alpha1,3/4-FUT
247
-
alpha1,3FucT IV
247
-
alpha1,3FucT IX
247
-
alpha1,3FucT V
247
-
alpha1,3FucT VI
247
-
alpha1,3FucT VII
247
-
alpha3/4 fucosyltransferase 3
247
-
alpha3/4 fucosyltransferases
247
-
alpha3/4FucT
267765, 268842
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alpha4-fucosyltransferase
-
-
-
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alpha4-FucT
-
-
-
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alphaFucT
247
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beta-acetylglucosaminylsaccharide fucosyltransferase
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blood group Lewis alpha-4-fucosyltransferase
-
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blood-group substance Lea-dependent fucosyltransferase
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-
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FT6
247
-
FT7
247
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Fuc-T
247
-
Fuc-TIII
-
-
-
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fucosyltransferase
268839, 268842
-
fucosyltransferase 3
267765
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fucosyltransferase 5
268839
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fucosyltransferase 6
268842
-
fucosyltransferase 7
277743
-
fucosyltransferase IV
247
-
fucosyltransferase V
247
-
fucosyltransferase VI
247
-
fucosyltransferase VII
277743
-
fucosyltransferase, guanosine diphosphofucose-beta-acetylglucosaminylsaccharide 4-alpha-L-
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-
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fucosyltransferase, guanosine diphosphofucose-glycoprotein 4-alpha-
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-
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FucT III
247
isoform
FucT IV
247
-
FucT IX
247
-
FucT V
247
isoform
FucT VI
FucT VII
277743
-
FucT-II
-
-
-
-
FucT-III
FucT-V
FucT-VI
FucT-VII
277743
-
FucTIII
-
-
-
-
FUT III
247
-
FUT10
FUT11
FUT4
247
-
FUT5
247
; isoform
Fut9
247
-
GDP-Fuc: beta-D-Galp-(1-3/4)-beta-D-GlcpNAc-(1-4'/3')-alpha-fucosyltransferase
247
-
guanosine diphosphofucose-glycoprotein 4-alpha-L-fucosyltransferase
-
-
-
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Lewis alpha-3-fucosyltransferase
268842
FUT6 gene
Lewis alpha-3/4-fucosyltransferase
Lewis alpha1-3/4 fucosyltransferase
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Lewis blood group alpha1-3/4 fucosyltransferase
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Lewis(Le) blood group gene-dependent alpha-3/4-L-fucosyltransferase
-
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sFUT5
247
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sperm fucosyltransferase-5
247
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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hexosyl group transfer
SYSTEMATIC NAME
IUBMB Comments
GDP-beta-L-fucose:beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R 4I-alpha-L-fucosyltransferase
This enzyme is the product of the Lewis blood group gene. Normally acts on a glycoconjugate where R (see reaction) is a glycoprotein or glycolipid. Although it is a 4-fucosyltransferase, it has a persistent 3-fucosyltransferase activity towards the glucose residue in free lactose. This enzyme fucosylates on O-4 of an N-acetylglucosamine that carries a galactosyl group on O-3, unlike EC 2.4.1.152, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, which fucosylates on O-3 of an N-acetylglucosamine that carries a galactosyl group on O-4. Enzymes catalysing the 4-alpha-fucosylation of the GlcNAc in beta-D-Gal-(1->3)-beta-GlcNAc sequences (with some activity also as 3-alpha-fucosyltransferases) are present in plants, where the function in vivo is the modification of N-glycans. In addition, the fucTa gene of Helicobacter strain UA948 encodes a fucosyltransferase with both 3-alpha- and 4-alpha-fucosyltransferase activities.
CAS REGISTRY NUMBER
COMMENTARY hide
37277-69-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-fucose + Fuc-alpha-1,2Gal-beta-1,3GlcNAc-sp-biotin
GDP + Fuc-alpha-1,2Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc-sp-biotin
show the reaction diagram
-
type 1 reaction
-
-
?
GDP-fucose + Fuc-alpha-1,2Gal-beta-1,4GlcNAc-sp-biotin
GDP + Fuc-alpha-1,2Gal-beta-1,4(Fuc-alpha-1,3)GlcNAc-sp-biotin
show the reaction diagram
-
type 2 reaction
-
-
?
GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R
GDP + 1,3-beta-D-galactosyl-(alpha-(1,4)-L-fucosyl)-N-acetyl-D-glucosaminyl-R
show the reaction diagram
Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
Fucalpha(1-2)Galbeta(1-3)[Fucalpha(1-4)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
show the reaction diagram
-
-
-
-
?
Fucalpha(1-2)Galbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
Fucalpha(1-2)Galbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
show the reaction diagram
-
-
-
-
?
Galbeta(1-3)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
Galbeta(1-3)[Fucalpha(1-4)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
show the reaction diagram
-
-
-
-
?
Galbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
Galbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + asialofetuin
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R
GDP + beta-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->4)]-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
isoform Fut3 predominantly exhibits alpha1-4 fucosyltransferase activity
-
-
?
GDP-beta-L-fucose + beta-D-Galp-(1,3)-beta-D-GlcNAcp-O-(CH2)6-NH-dansyl
GDP + beta-D-Galp-(1,3)[alpha-L-Fuc-(1,4)]-beta-D-GlcNAcp-O-(CH2)6-NH-dansyl
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + Fucalpha(1->2)Galbeta(1->3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + Fucalpha1,2Galbeta(1->4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + Galbeta(1->3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + Galbeta(1->4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + NeuAcalpha(2->3)Galbeta(1->3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + NeuAcalpha(2->3)Galbeta(1->4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + NeuAcalpha2-3Galbeta1-4Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide
?
show the reaction diagram
-
the enzyme catalyzes the fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylactosamine acceptor leading to two isomeric monofucosyl antigens, VIM2 and sialyl-Lex
-
-
?
GDP-beta-L-fucose + NeuAcalpha2-3Galbeta1-4Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide
GDP + NeuAcalpha2-3Galbeta1-4[Fucalpha1-3]Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide
show the reaction diagram
-
the enzyme catalyzes the fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylactosamine acceptor leading to two isomeric monofucosyl antigens, VIM2 and sialyl-Lex
i.e. sialyl-Lex, main product of FucT-VI, i.e. VIM2, main product of FucT-V
-
?
GDP-beta-L-fucose labelled with ATTO 550 + N-acetyllactosamine labelled with ATTO 647N
?
show the reaction diagram
-
-
-
-
?
GDP-fucose + 2-O-MeGal-beta-1,3GlcNAc
GDP + 2-O-MeGal-beta-1,3(Fuc-alpha-1,4)GlcNAc
show the reaction diagram
GDP-fucose + 2-O-MeGal-beta-1,4GlcNAc
GDP + 2-O-MeGal-beta-1,4(Fuc-alpha-1,3)GlcNAc
show the reaction diagram
100% activity
-
-
?
GDP-fucose + Fetuin triantennary asialo agalacto glycoprotein
GDP + ?
show the reaction diagram
GDP-fucose + Fetuin triantennary asialo glycoprotein
GDP + ?
show the reaction diagram
GDP-fucose + Fuc-alpha-1,2Gal-beta-1,3GlcNAc-sp-biotin
GDP + Fuc-alpha-1,2Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc-sp-biotin
show the reaction diagram
-
type 1 reaction
-
-
?
GDP-fucose + Fuc-alpha-1,2Gal-beta-1,4GlcNAc-sp-biotin
GDP + Fuc-alpha-1,2Gal-beta-1,4(Fuc-alpha-1,3)GlcNAc-sp-biotin
show the reaction diagram
-
type 2 reaction
-
-
?
GDP-fucose + Gal-beta-1,3GlcNAc
GDP + Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc
show the reaction diagram
-
-
-
-
?
GDP-fucose + Gal-beta-1,3GlcNAc-beta-1,3Gal-beta-1,4Glc
GDP + Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc-beta-1,3Gal-beta-1,4Glc
show the reaction diagram
-
-
-
-
?
GDP-fucose + Gal-beta-1,3GlcNAc-O-(CH2)3NHCO(CH2)5NH-biotin
GDP + Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc-O-(CH2)3NHCO(CH2)5NH-biotin
show the reaction diagram
-
-
-
-
?
GDP-fucose + Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin
GDP + Gal-beta-1,3[alpha-L-fucosyl-(1,4)]GlcNAcO(CH2)3NHCO(CH2)5NH-biotin
show the reaction diagram
-
-
-
-
?
GDP-fucose + Gal-beta-1,4-Glc
GDP + Gal-beta-1,4(Fuc-alpha-1,3)Glc
show the reaction diagram
-
-
-
-
?
GDP-fucose + Gal-beta-1,4GlcNAc
GDP + Gal-beta-1,4(Fuc-alpha-1,3)GlcNAc + Gal-beta-1,4(Fuc-alpha-1,2)GlcNAc
show the reaction diagram
-
enzyme activity is divided into 66% alpha-1,3FucT and 34% alpha-1,2FucT
-
-
?
GDP-fucose + Gal-beta-1,4GlcNAc-beta-1-R
GDP + Gal-beta-1,4(fuc-alpha-1,3)GlcNAc-beta-1-R
show the reaction diagram
-
-
-
-
?
GDP-fucose + GalNAc-beta-1,4GlcNAc-beta-O-Bn
GDP + GalNAc-beta-1,4(Fuc-alpha-1,3)GlcNAc-beta-O-Bn
show the reaction diagram
GDP-fucose + GlcNAc-beta-1,4GlcNAc-beta-1,4GlcNAc
?
show the reaction diagram
acivity not determined
-
-
?
GDP-fucose + GlcNAc-beta-1,4GlcNAc-beta-1,4GlcNAc
GDP + ?
show the reaction diagram
63.8% acivity
-
-
?
GDP-fucose + GlcNAc-beta-1,4GlcNAc-beta-1,4GlcNAc-beta-1,4GlcNAc
?
show the reaction diagram
acivity not determined
-
-
?
GDP-fucose + GlcNAc-beta-1,4GlcNAc-beta-1,4GlcNAc-beta-1,4GlcNAc
GDP + ?
show the reaction diagram
61.5% acivity
-
-
?
GDP-fucose + GlcNAc-beta-1,4GlcNAc-beta-O-Bn
GDP + GlcNAc-beta-1,4(Fuc-alpha-1,3)GlcNAc-beta-O-Bn
show the reaction diagram
GDP-fucose + LacNAc
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + (Gal-3-O-Me)beta(1-3)(GlcNAc-6-O-Me)beta-O-Me
GDP + Fucalpha(1-3)(Gal-3-O-Me)beta(1-3)(GlcNAc-6-O-Me)beta-O-Me
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R
GDP + 1,3-beta-D-galactosyl-(alpha-(1,4)-L-fucosyl)-N-acetyl-D-glucosaminyl-R
show the reaction diagram
-
the enzyme catalyzes the synthesis of fucosylated Lewis motifs that are associated with cell-adhesion events and are differentially expressed during cell differentiation
-
-
-
GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R
GDP + 1,3-beta-D-galactosyl-(alpha-1,4-L-fucosyl)-N-acetyl-D-glucosaminyl-R
show the reaction diagram
GDP-L-fucose + 2'-fucosyllactose
GDP + ?
show the reaction diagram
GDP-L-fucose + 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn
GDP + 2-O-MeGalbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Bn
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + 3-O-MeGalbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
GDP + 3-O-MeGalbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
show the reaction diagram
-
16.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 17.8% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + 3-O-sulfoGalbeta(1,3)GlcNAcbeta(1,3)Galbeta-O-Al
GDP + 3-O-sulfoGalbeta(1,3)(Fucalpha(1,4))GlcNAcbeta(1,3)Galbeta-O-Al
show the reaction diagram
-
64.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 82.2% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + 3-O-sulfoGalbeta(1,3)GlcNAcbeta-O-Al
GDP + 3-O-sulfoGalbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Al
show the reaction diagram
-
64.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 62.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + 3-O-sulfoGalbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
GDP + 3-O-sulfoGalbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
show the reaction diagram
-
10.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 14.0% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + 6-O-sulfoGalbeta(1,3)GlcNAcbeta-O-Al
GDP + 6-O-sulfoGalbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Al
show the reaction diagram
-
3.1% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 3.5% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + alpha(2,3)-sialyllactosamine
GDP + ?
show the reaction diagram
-
56% of the activity with Galbeta(1,4)GlcNAc
-
-
?
GDP-L-fucose + ancrod
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + asialo ancrod
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + asialofetuin
GDP + ?
show the reaction diagram
GDP-L-fucose + fetuin triantennary glycopeptide
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + formic acid (2R,3R,4S,5R,6R)-2-((2R,3R,4R,5R,6R)-3-acetylamino-5-hydroxy-2-methoxy-6-methylaminomethyl-tetrahydro-pyran-4-yloxy)-4,5-dihydroxy-6-hydroxymethyl-tetrahydro-pyran-3-yl ester
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Fuc-alpha1-2Galbeta1-4GlcNAc-octyl
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc
GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAc
show the reaction diagram
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc-(CH2)5-NH-biotin
GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-(CH2)5-NH-biotin
show the reaction diagram
-
193% of the activity with Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
-
-
?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc-R
GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-R
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc
GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta(1,3)Galbeta(1,4)Glc
show the reaction diagram
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)Glc
GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))Glc
show the reaction diagram
-
34.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 38.7% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc
GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc
show the reaction diagram
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
show the reaction diagram
-
3% of the activity with with Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin, enzyme expressed in Sf9 cells
-
-
?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc-(CH2)5-NH-biotin
GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc-(CH2)5-NH-biotin
show the reaction diagram
-
9.3% of the activity with Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
-
-
?
GDP-L-fucose + Galalpha(1,3)Galbeta(1,3)GlcNAc-R
GDP + Galalpha(1,3)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-R
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galalpha(1,3)Galbeta(1,3)GlcNAcbeta-O-Naph
GDP + Galalpha(1,3)Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Naph
show the reaction diagram
-
42.6 of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 46.0% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Galbeta(1,3)(6-O-sulfo)GlcNAcbeta(1,3)Galbeta-O-Al
GDP + ?
show the reaction diagram
-
15.5% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 19.4% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Galbeta(1,3)GlcNAc
GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc
show the reaction diagram
GDP-L-fucose + Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1,3)GlcNAc-R
GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc-R
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc
GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta(1,3)Galbeta(1,4)Glc
show the reaction diagram
-
20% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
-
?
GDP-L-fucose + Galbeta(1,3)GlcNAcbeta-O-Bn
GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Bn
show the reaction diagram
-
79.1% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 83.8% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Galbeta(1,4)-(5-thioGlc)
GDP + ?
show the reaction diagram
-
51% of the activity with Galbeta(1,4)GlcNAc
-
-
?
GDP-L-fucose + Galbeta(1,4)Glc
GDP + Galbeta(1,4)(Fucalpha(1,3))Glc
show the reaction diagram
GDP-L-fucose + Galbeta(1,4)GlcNAc
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAc
show the reaction diagram
GDP-L-fucose + Galbeta(1,4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
show the reaction diagram
-
2% of the activity with Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin, enzyme expressed in Sf9 cells
-
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,2)Man
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,2)Man
show the reaction diagram
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,3)Galbeta(1,4)Glc
show the reaction diagram
-
33% of the activity with Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,6)(3-O-MeGalbeta(1,3))GalNAcalpha-O-Bn
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(3-O-MeGalbeta(1,3))GalNAcalpha-O-Bn
show the reaction diagram
-
2.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 1.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,6)(3-O-sulfoGalbeta(1,3))-GalNAcalpha-O-Bn
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(3-O-sulfoGalbeta(1,3))-GalNAcalpha-O-Bn
show the reaction diagram
-
1.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 1.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
show the reaction diagram
-
2.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 1.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta-O-Bn
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta-O-Bn
show the reaction diagram
-
3.1% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 2.5% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + Galbeta(1,4)GlcNAcbetaOallyl
GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbetaOallyl
show the reaction diagram
-
64% of the activity with Galbeta(1,4)GlcNAc
-
-
-
GDP-L-fucose + Galbeta(1,4)Glucal
GDP + Galbeta(1,4)(Fucalpha(1,3))Glucal
show the reaction diagram
-
10% of the activity with Galbeta(1,4)GlcNAc
-
-
?
GDP-L-fucose + Galbeta(1,6)Galbeta(1,4)Glc
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-3)GlcNAcbeta-O-Me
GDP + Fucalpha(1-3)Galbeta(1-3)GlcNAcbeta-O-Me
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta1-4GlcNAc-4-nitrophenyl
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + GalNAcbeta(1,3)(6-O-sulfo)GlcNAcbeta-O-Me
GDP + ?
show the reaction diagram
-
6.2 of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 8.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + GalNAcbeta1-4GlcNAc-diaminopimelyl
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + GalNAcbeta1-4GlcNAc-octyl
GDP + ?
show the reaction diagram
-
shows the highest catalytic activity as an acceptor substrate
-
-
?
GDP-L-fucose + IL-4 receptor
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Lewis antigen
GDP + fucosylated Lewis antigen
show the reaction diagram
GDP-L-fucose + N-((1R,3R,5R,6R,7S,8R,13R,15R,16R,17S)-6,7,17-trihydroxy-5-hydroxymethyl-15-methoxy-2,4,9,11,14-pentaoxa-tricyclo[11.3.1.0 3,8]heptadec-16-yl)-acetamide
GDP + N-[(1R,3R,5R,6S,7S,8R,13R,15R,16R,17S)-6,17-dihydroxy-5-hydroxymethyl-15-methoxy-7-((2R,3R,4S,5R,6S)-3,4,5-trihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-2,4,9,11,14-pentaoxa-tricyclo[11.3.1.0 3,8]heptadec-16-yl]-acetamide
show the reaction diagram
-
low activity with FucT IV and FucT VI
-
-
?
GDP-L-fucose + N-((1R,3R,5R,6R,7S,8R,13R,15R,16R,17S)-6,7,17-trihydroxy-5-hydroxymethyl-15-methoxy-2,4,9,11,14-pentaoxa-tricyclo[11.3.1.03,8]heptadec-16-yl)-acetamide
GDP + N-[(1R,3R,5R,6S,7S,8R,13R,15R,16R,17S)-6,17-dihydroxy-5-hydroxymethyl-15-methoxy-7-((2R,3R,4S,5R,6S)-3,4,5-trihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-2,4,9,11,14-pentaoxa-tricyclo[11.3.1.0 3,8]heptadec-16-yl]-acetamide
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + N-((1R,3R,5R,6R,7S,8R,14R,16R,17R,18R)-6,7,18-trihydroxy-5-hydroxymethyl-16-methoxy-11-oxo-2,4,9,15-tetraoxa-12-aza-tricyclo[12.3.1.03,8]octadec-17-yl)-acetamide
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + N-((1R,3R,5R,6R,7S,8R,14R,16R,17R,18S)-6,7,18-trihydroxy-5-hydroxymethyl-16-methoxy-2,4,9,12,15-pentaoxa-tricyclo[12.3.1.03,8]octadec-17-yl)-acetamide
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + N-acetyllactosamine
GDP + alpha-1,3-L-fucosyl-N-acetyllactosamine
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + NeuAc(2,3)Galbeta(1,4)Glucal
GDP + NeuAc(2,3)Galbeta(1,4)(Fucalpha(1,3))Glucal
show the reaction diagram
-
330% of the activity with Galbeta(1,4)GlcNAc
-
-
?
GDP-L-fucose + NeuAc(2,6)Galbeta(1,4)GlcNAc
GDP + NeuAc(2,6)Galbeta(1,4)(Fucalpha(1,3))GlcNAc
show the reaction diagram
-
13% of the activity with Galbeta(1,4)GlcNAc
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
GDP + NeuAcalpha(2,3)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,3)GlcNAc-(CH2)5-NH-biotin
GDP + NeuAcalpha(2,3)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-(CH2)5-NH-biotin
show the reaction diagram
-
57% of the activity with Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,3)GlcNAcbeta-O-Bn
GDP + NeuAcalpha(2,3)Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Bn
show the reaction diagram
-
55.8% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 64.4% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAc
GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAc
show the reaction diagram
-
620% of the activity with Galbeta(1,4)GlcNAc
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-OMe
GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-OMe
show the reaction diagram
-
24.2% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 12.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAcbeta-O-Bn
GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta-O-Bn
show the reaction diagram
-
6.2% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 7.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB
-
-
?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAcbetaOallyl
GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAcbetaOallyl
show the reaction diagram
-
380% of the activity with Galbeta(1,4)GlcNAc
-
-
?
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
show the reaction diagram
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)AspNHCO-biotin + GDP-L-fucose
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)AspNHCO-biotin + GDP
show the reaction diagram
-
-
-
?
NeuAcalpha(2-3)Galbeta(1-3)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
NeuAcalpha(2-3)Galbeta(1-3)[Fucalpha(1-4)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
show the reaction diagram
-
-
-
-
?
NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
NeuAcalpha(2-3)Galbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
show the reaction diagram
-
-
-
-
?
pyridylamine-lacto-N-neotetraose + GDP-fucose
GDP + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R
GDP + 1,3-beta-D-galactosyl-(alpha-(1,4)-L-fucosyl)-N-acetyl-D-glucosaminyl-R
show the reaction diagram
GDP-beta-L-fucose + asialofetuin
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R
GDP + beta-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->4)]-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
isoform Fut3 predominantly exhibits alpha1-4 fucosyltransferase activity
-
-
?
GDP-beta-L-fucose + NeuAcalpha2-3Galbeta1-4Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide
?
show the reaction diagram
-
the enzyme catalyzes the fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylactosamine acceptor leading to two isomeric monofucosyl antigens, VIM2 and sialyl-Lex
-
-
?
GDP-fucose + Gal-beta-1,4GlcNAc-beta-1-R
GDP + Gal-beta-1,4(fuc-alpha-1,3)GlcNAc-beta-1-R
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R
GDP + 1,3-beta-D-galactosyl-(alpha-(1,4)-L-fucosyl)-N-acetyl-D-glucosaminyl-R
show the reaction diagram
-
the enzyme catalyzes the synthesis of fucosylated Lewis motifs that are associated with cell-adhesion events and are differentially expressed during cell differentiation
-
-
-
GDP-L-fucose + Lewis antigen
GDP + fucosylated Lewis antigen
show the reaction diagram
-
on the cell surface of hepatocarcinoma cells H7721
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
stimulates
Cd2+
-
stimulates
Cu2+
-
no significant activity is detected
Ni2+
-
stimulates
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(-)-epigallocatechin-3-gallate
time-dependent, irreversible
2'-fucosyllactose
-
-
2,3-Dihydroxybenzoic acid
-
2,4-dihydroxybenzoic acid
-
2,5-dihydroxybenzoic acid
-
3,4,5-Trihydroxybenzoic acid
gallic acid, time-dependent, irreversible
3,4,5-trimethoxybenzoic acid
-
3,4-dihydroxybenzoic acid
-
3,4-dimethoxybenzoic acid
-
3,5-dimethoxy 4-hydroxybenzoic acid
syringic acid
4-O-beta-D-galactopyranosyl-D-glucitol
-
competitive with respect to lactose
9-(5'-O-[(alpha,beta-L-fucopyranosyl)oxycarbonylaminosulfonyl]-beta-D-ribofuranosyl)-guanine
-
poor competitive inhibition
C-fucosyl analogon of GDP-fucose
-
-
carba-fucosyl analogon of GDP-fucose
-
-
dGDP
-
about 76% inhibition at 0.4 mM
ellagic acid
-
Galbeta(1,3)GalNAc
-
-
Galbeta(1,4)(3-deoxy)GlcNAcbetaOallyl
-
IC50: 710 mM
Galbeta(1,4)deoxynojirimycin
-
IC50: 8 mM
GDP-6-amino-beta-L-fucose
-
78% inhibition at 0.4 mM
GDP-Man
-
IC50: 2 mM
GMP
-
competitive with respect to GDP-fucose
GTP
-
about 80% inhibition at 0.4 mM
IDP
-
about 30% inhibition at 0.4 mM
lacto-N-fucopentaitol I
-
-
methyl gallate
-
propyl gallate
-
unsaturated carba-fucosyl analogon of GDP-fucose
-
-
-
[1-(beta-D-fucopyranosyl)-1H-1,2,3-triazole-4-yl]methylguanosine diphosphate
-
about 70% inhibition at 0.4 mM
[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazole-4-yl] methylguanosine diphosphate
-
about 73% inhibition at 0.4 mM
[1-(beta-D-lactosyl)-1H-1,2,3-triazole-4-yl]methylguanosine diphosphate
-
about 45% inhibition at 0.4 mM
[1-(beta-L-fucopyranosyl)-1H-1,2,3-triazole-4-yl]methylguanosine diphosphate
-
about 60% inhibition at 0.4 mM
additional information
-
not inhibited by L-fucose, GMP and ADP
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2 - 2.5
Fuc-alpha-1,2Gal-beta-1,3GlcNAc-sp-biotin
0.034 - 0.046
GDP-fucose
0.12 - 2.7
(Gal-3-O-Me)beta(1-3)(GlcNAc-6-O-Me)beta-O-Me
11
2'-fucosyllactose
-
-
0.16 - 0.4
2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn
0.047 - 0.1
3-O-sulfoGalbeta(1,3)GlcNAcbeta-O-Al
0.045
ancrod
-
enzyme form FTB
-
0.167
asialo ancrod
-
enzyme form FTB
-
0.63
fetuin triantennary asialoglycopeptide
-
enzyme form FTB
-
1.43
fetuin triantennary glycopeptide
-
enzyme form FTB
-
0.075 - 1.3
Formic acid (2R,3R,4S,5R,6R)-2-((2R,3R,4R,5R,6R)-3-acetylamino-5-hydroxy-2-methoxy-6-methylaminomethyl-tetrahydro-pyran-4-yloxy)-4,5-dihydroxy-6-hydroxymethyl-tetrahydro-pyran-3-yl ester
0.1 - 1.3
Fuc-alpha-1,2Gal-beta-1,4GlcNAc-sp-biotin
0.14
Fuc-alpha1-2Galbeta1-4GlcNAc-octyl
-
-
0.1 - 3.8
Fucalpha(1,2)Galbeta(1,3)GlcNAc
0.8
Fucalpha(1,2)Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
-
1.52
Fucalpha(1,2)Galbeta(1,4)Glc
-
enzyme form FTB
0.5 - 0.7
Fucalpha(1,2)Galbeta(1,4)GlcNAc
0.7 - 3.3
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin
0.6 - 12.7
Galbeta(1,3)GlcNAc
0.76 - 0.87
Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
2.4
Galbeta(1,3)GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
-
0.012
Galbeta(1,4)(5-thioGlc)
-
-
0.5
Galbeta(1,4)Glc
-
-
0.035 - 8.1
Galbeta(1,4)GlcNAc
0.4
Galbeta(1,4)GlcNAcbeta(1,2)Man
-
-
3.8
Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
-
0.016
Galbeta(1,4)GlcNAcbetaOallyl
-
-
0.034
Galbeta(1,4)Glucal
-
-
12
Galbeta(1,6)Galbeta(1,4)Glc
-
-
0.35 - 1.2
Galbeta(1-3)GlcNAcbeta-O-Me
3.6
Galbeta1-4GlcNAc-4-nitrophenyl
-
-
3.8
GalNAcbeta1-4GlcNAc-diaminopimelyl
-
-
0.27
GalNAcbeta1-4GlcNAc-octyl
-
-
0.016
GDP
-
-
0.1145
GDP-D-fucose
-
mutant enzyme D336A
0.03 - 0.0504
GDP-fucose
0.005 - 0.035
GDP-L-fucose
0.15 - 0.154
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-)AspNHCO-biotin
0.06
GMP
-
-
3 - 25
N-((1R,3R,5R,6R,7S,8R,13R,15R,16R,17S)-6,7,17-trihydroxy-5-hydroxymethyl-15-methoxy-2,4,9,11,14-pentaoxa-tricyclo[11.3.1.0 3,8]heptadec-16-yl)-acetamide
1.1 - 11
N-((1R,3R,5R,6R,7S,8R,13R,15R,16R,17S)-6,7,17-Trihydroxy-5-hydroxymethyl-15-methoxy-2,4,9,11,14-pentaoxa-tricyclo[11.3.1.03,8]heptadec-16-yl)-acetamide
0.61 - 16
N-((1R,3R,5R,6R,7S,8R,14R,16R,17R,18R)-6,7,18-trihydroxy-5-hydroxymethyl-16-methoxy-11-oxo-2,4,9,15-tetraoxa-12-aza-tricyclo[12.3.1.03,8]octadec-17-yl)-acetamide
0.4 - 5
N-((1R,3R,5R,6R,7S,8R,14R,16R,17R,18S)-6,7,18-Trihydroxy-5-hydroxymethyl-16-methoxy-2,4,9,12,15-pentaoxa-tricyclo[12.3.1.03,8]octadec-17-yl)-acetamide
0.67 - 2.5
NeuAcalpha(2,3)Galbeta(1,3)GlcNAcbeta-O-Bn
0.1 - 0.28
NeuAcalpha(2,3)Galbeta(1,4)GlcNAc
0.77 - 3.3
NeuAcalpha(2,3)Galbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-OMe
0.064
NeuAcalpha(2,3)Galbeta(1,4)Glucal
-
-
0.07
NeuAcalpha(2,6)Galbeta(1,4)GlcNAc
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.02 - 8.02
Fucalpha(1,2)Galbeta(1,3)GlcNAc
0.1 - 0.3
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin
0.009 - 0.4
Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0007 - 0.0022
(-)-epigallocatechin-3-gallate
7
2'-fucosyllactose
-
-
0.115
2,5-dihydroxybenzoic acid
presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose
0.00006 - 0.008
3,4,5-Trihydroxybenzoic acid
0.137
3,4-dihydroxybenzoic acid
presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose
17
4-O-beta-D-galactopyranosyl-D-glucitol
-
-
0.889
C-fucosyl analogon of GDP-fucose
-
-
0.0671
carba-fucosyl analogon of GDP-fucose
-
-
0.0503
dGDP
-
at pH 4.5 and 37°C
0.0012
ellagic acid
presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose
0.0779
GDP
-
at pH 4.5 and 37°C
0.102
GDP-6-amino-beta-L-fucose
-
at pH 4.5 and 37°C
0.0807
GTP
-
at pH 4.5 and 37°C
0.6
lacto-N-fucopentaitol I
-
-
0.008
methyl gallate
presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose
0.25
NEM
-
enzyme form FTA and FTB
0.045
propyl gallate
presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose
0.0256
unsaturated carba-fucosyl analogon of GDP-fucose
-
-
-
0.19
[1-(beta-D-fucopyranosyl)-1H-1,2,3-triazole-4-yl]methylguanosine diphosphate
-
at pH 4.5 and 37°C
0.186
[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazole-4-yl] methylguanosine diphosphate
-
at pH 4.5 and 37°C
0.137
[1-(beta-L-fucopyranosyl)-1H-1,2,3-triazole-4-yl]methylguanosine diphosphate
-
at pH 4.5 and 37°C
additional information
additional information
2,3-dihydroxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose, 2,4-dihydroxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose, 3,5-dimethoxy 4-hydroxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose, 3,4-dimethoxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose, 3,4,5-trimethoxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20
9-(5'-O-[(alpha,beta-L-fucopyranosyl)oxycarbonylaminosulfonyl]-beta-D-ribofuranosyl)-guanine
Homo sapiens
-
-
0.049
dGDP
Homo sapiens
-
at pH 4.5 and 37°C
710
Galbeta(1,4)(3-deoxy)GlcNAcbetaOallyl
Homo sapiens
-
IC50: 710 mM
8
Galbeta(1,4)deoxynojirimycin
Homo sapiens
-
IC50: 8 mM
0.059
GDP
Homo sapiens
-
at pH 4.5 and 37°C
0.104
GDP-6-amino-beta-L-fucose
Homo sapiens
-
at pH 4.5 and 37°C
2
GDP-Man
Homo sapiens
-
IC50: 2 mM
0.094
GTP
Homo sapiens
-
at pH 4.5 and 37°C
0.134
[1-(beta-D-fucopyranosyl)-1H-1,2,3-triazole-4-yl]methylguanosine diphosphate
Homo sapiens
-
at pH 4.5 and 37°C
0.247
[1-(beta-D-galactopyranosyl)-1H-1,2,3-triazole-4-yl] methylguanosine diphosphate
Homo sapiens
-
at pH 4.5 and 37°C
0.139
[1-(beta-L-fucopyranosyl)-1H-1,2,3-triazole-4-yl]methylguanosine diphosphate
Homo sapiens
-
at pH 4.5 and 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.061
-
-
1.13
-
reaction with lacto-N-fucopentaose I
2.05
-
reaction with 2'-fucosyllactose
72
-
enzyme expressed in Trichoplusia ni
567
-
enzyme expressed in Sf9 cells
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
-
in the absence of Mn2+
6
-
assay at
6.5
-
assay at
7 - 7.8
-
-
7
-
in the presence of Mn2+
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
in the presence of Mn2+
6.2 - 8.5
-
pH 6.2: about 70% of maximal activity, pH 8.5: about 90% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
; low expression of FUT1-6 and FUT9, highest expression of FUT10, FUT11 is absent
Manually annotated by BRENDA team
FUT10-479, adult tissue with low level
Manually annotated by BRENDA team
adult tissue with high level
Manually annotated by BRENDA team
FUT10-391 and FUT10-419
Manually annotated by BRENDA team
-
cystic fibrosis airway epithelial cell
Manually annotated by BRENDA team
adult tissue with high level
Manually annotated by BRENDA team
-
hepatocarcinoma cell, expression levels of alpha1,3FucT enzyme types in descending order: alphaFucT IV, III, VI, VI, VII, and IX
Manually annotated by BRENDA team
adult tissue with weak level
Manually annotated by BRENDA team
adult tissue with high level
Manually annotated by BRENDA team
-
; very low expression of FUT1, low expression of FUT2, higher expression of FUT4-6, highest expression of FUT3 and FUT10-11
Manually annotated by BRENDA team
fetal tissue with weak level, adult tissue with moderate level
Manually annotated by BRENDA team
fetal tissue, intensity similar to in the embryos
Manually annotated by BRENDA team
fetal and adult tissue with weak level
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
; very low expression of FUT1, FUT5 and FUT6, low expression of FUT2, higher expression of FUT9-11, FUT3 is absent, highest expression of FUT4
Manually annotated by BRENDA team
fetal tissue with weak level
Manually annotated by BRENDA team
-
FT3, FT6, and FT7
Manually annotated by BRENDA team
adult tissue with weak level
Manually annotated by BRENDA team
-
FT4 and FT9
Manually annotated by BRENDA team
adult tissue with high level
Manually annotated by BRENDA team
fetal tissue, intensity similar to in the embryos
Manually annotated by BRENDA team
fetal tissue, intensity similar to in the embryos
Manually annotated by BRENDA team
adult tissue with high level
Manually annotated by BRENDA team
adult tissue with moderate level
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
fusion proteins PIR1-HA-FUT6 and PIR2-FLAG-FUT6
Manually annotated by BRENDA team
-
Golgi type II membrane protein
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
sperm isoform FUT5 pretreatment or blocking suppresses the protective effect of OE-E6/E7 membrane proteins on sperm motility
metabolism
-
isoform Fut3 expression drives sialyl Lewis A synthesis in V6 variant of CD44-expressing cells
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
FUT3_HUMAN
361
0
42117
Swiss-Prot
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42117
-
x * 42117, calculation from nucleotide sequence
41800
-
SDS-PAGE, immunoblot
41859
-
1 * 41859, calculation from nucleotide sequence
51000
-
x * 51000 + x * 53000, SDS-PAGE
53000
-
x * 51000 + x * 53000, SDS-PAGE
additional information
-
disulfide bonds in FucT III occur between Cys residues Cys81 and Cys338 and between Cys91 and Cys341 at the N and C termini of the catalytic domain, bringing theses ends close together in space
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 41859, calculation from nucleotide sequence
additional information
-
the enzyme is present in an equilibrium of monomer/dimer in the trans-Golgi/trans-Golgi-network of transfected BHK cells
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
additional information
-
the enzyme expressed in Tn cell line has a lower global charge, possibly due to post-translational modifications, such as phosphorylatopm or sulfation
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
W111A
-
alpha-1,4-activity is decreased to 10-20% of the wild type activity
W111F
-
alpha-1,4-activity is decreased to 42% of the wild type activity
W111Y
-
alpha-1,4-activity is decreased to 58% of the wild type activity
D112E
-
muation decreases activity of the enzyme and does not interfere with H-type 1/H-type 2 acceptors
D125E
-
site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity
D336A
-
fucosyltransferase III mutant enzyme shows reduced activity with a variety of acceptors, 40fold reduction in activity for Fucalpha(1,2)Galbeta(1,3)GlcNAc. 4fold reduction affinity for GDP-fucose. The single amino acid site Asp336 of FucT III and Ala349 of FucT V constitutes the only difference in the sequence of FucT III and V over the final 210 COOH-terminal amino acid residues, impacts the acceptor substrate profiles of FucT III and FuvT V
E111D
-
site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity
I126V
-
site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity
K73T
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
K73T/E111D
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
K73T/I75V/T87A
-
insertion mutagenesis of FucT-VI, the mutant shows altered substrate specificity compared to the wild-type enzyme
K73T/R110W
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
K73T/R110W/E111D/V112I
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
K97S
-
site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity
R110H
-
less than 10% of the wild type alpha-1,3-activity and undetectable alpha-1,4-activity
R110K
-
less than 10% of the wild type alpha-1,3-activity and undetectable alpha-1,4-activity
R110N
-
less than 10% of the wild type alpha-1,3-activity and undetectable alpha-1,4-activity
R110Q
R110W
R110W/E111D/V112I
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
R96S
-
site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity
S110R
-
site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity
S111K
-
site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity
T87K
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
T87K/D125E
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
T87K/V89I/A101T
-
insertion mutagenesis of FucT-V, the mutant shows altered substrate specificity compared to the wild-type enzyme
T87K/W124R
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
T87K/W124R/D125E/I126V
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
V112I
-
site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity
W111R
-
the mutation changes the specificity for fucose transfer from H-type 1 to H-type 2 acceptors
W11R/D112E
-
the mutation changes the specificity for fucose transfer from H-type 1 to H-type 2 acceptors. Increased type 2 activity compared to mutant W111R
W124A
-
alpha-1,4-activity is decreased to 20% of the wild type activity
W124F
-
alpha-1,4-activity is decreased to 17% of the wild type activity
W124R
W124R/D125E/I126V
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
W124V
-
undetectable alpha-1,4-activity
W124Y
-
alpha-1,4-activity is decreased to 52% of the wild type activity
additional information
-
construction of several chimeric mutants by domain swappig, overview
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50% glycerol, protein concentration 0.03 mg/ml
-
-80°C, several months
-
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
native enzyme by preparation of microsome and Golgi membrane fractions, ultracentrifugation, and affinity chromatography
anion exchange-chromatography on a CM-Sepharose column, followed by an affinity chromatography on a GDP Fractogel column
-
Mono Q column chromatography and Sephadex G-25 gel filtration
-
native enzyme by preparation of microsome and Golgi membrane fractions, ultracentrifugation, and affinity chromatography
secretory variant of enzyme
-
two molecular forms: FTA and FTB
-
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
construction of plasmids encoding soluble forms of the recombinant human FucT-III where the human IL-2 sequence is linked to Ala47 or Val-36 of the FucT-III and expression in stable transfected BHK-21 cell lines
-
expression in BHK-21B cells
-
expression in COS cells
-
expression in HEK 293 cells
expression in Saccharomyces cerevisiae, construction of fusion genes PIR1-HA-FUT6 and PIR2-FLAG-FUT6
-
expression in Spodoptera frugiperda (Sf9) insect cells
-
expression in Spodoptera frugiperda SF-9 cells, the secreted activity SFT3 increases until the 6th day of culture when it reaches the value 1.9 mU x 10e-6 cells and 13.4 mg/l, whereas only 5% of activity is retained inside the cells
-
expression of a secreted form of Fuc-TIII, SFT3, in two insect cell lines, Spodoptera frugiperda and Trichoplusia ni using the baculovirus expression system. The enzyme from the Tn cell line has a lower global charge, possibly due to post-translational modifications, such as phosphorylation or sulfation
-
expression of alpha-1,3/4-fucosyltransferase in the gastric pit cell lineage of FVB/N transgenic mice
-
expression of wild-type and mutant enzyme in COS-7 cells
-
plasmid ProtA-FucT-VI, encoding a soluble chimaeric protein consisting of a part of protein A fused to the catalytic domain of FucT-VI, transfected into Cos-7 cells
-
stable expression of FucT IV and IV in CHO cells
-
transient expression of cDNA splice variants FUT10-391, FUT10-419, and FUT10-479, and GFP-tagged-FUT10 cDNA constructs from vector PCR3.1 in COS-7 Cells
transient expression of cDNA splice variants FUT11-476 and FUT11-492 from vector PCR3.1 in COS-7 Cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
decrease in FUT4 transcript levels in MKN45 confluent cells
-
FT3, FT6, and FT7 are markedly elevated in bone- and liver-metastatic prostate cancer and dictate synthesis of sialyl Lewis X and endothelial selectin ligands on metastatic prostate cancer cells
-
FT4 expression is 3- to 15fold lower in metastatic specimens
-
increase in mRNA levels of FUT5 and FUT6 in MKN45 confluent cells
-
the enzyme is upregulated 3fold in hepatocellular carcinoma cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
molecular biology
-
the stable system using the expression vector pIB/Vf-His-TOPO constitutes an advance for the large scale expression of glycosyltransferases and possibly other glycoproteins in insect cells
synthesis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wong, C.H.; Dumas, D.P.; Ichikawa, Y.; Koseki, K.; Danishefsky, S.J.; Weston, B.W.; Lowe, J.B.
Specificity, inhibition, and synthetic utility of a recombinant human alpha-1,3-fucosyltransferase
J. Am. Chem. Soc.
114
7321-7322
1992
Homo sapiens
-
Manually annotated by BRENDA team
Prieels, J.P.; Monnom, D.; Dolmans, M.; Beyer, T.A.; Hill, R.L.
Co-purification of the Lewis blood group N-acetylglucosaminide alpha 1-4 fucosyltransferase and an N-acetylglucosaminide alpha 1-3 fucosyltransferase from human milk
J. Biol. Chem.
256
10456-10463
1981
Homo sapiens
Manually annotated by BRENDA team
Palma, A.S.; Vila-Verde, C.; Pires, A.S.; Fevereiro, P.S.; Costa, J.
A novel plant alpha4-fucosyltransferase (Vaccinium myrtillus L.) synthesises the Lewisa adhesion determinant
FEBS Lett.
499
235-238
2001
Homo sapiens, Vaccinium myrtillus
Manually annotated by BRENDA team
Sousa, V.L.; Costa, M.T.; Palma, A.S.; Enguita, F.; Costa, J.
Localization, purification and specificity of the full-length membrane-bound form of human recombinant alpha 1,3/4-fucosyltransferase from BHK-21B cells
Biochem. J.
357
803-810
2001
Homo sapiens
Manually annotated by BRENDA team
Legault, D.J.; Kelly, R.J.; Natsuka, Y.; Lowe, J.B.
Human alpha(1,3/1,4)-fucosyltransferases discriminate between different oligosaccharide acceptor substrates through a discrete peptide fragment
J. Biol. Chem.
270
20987-20996
1995
Homo sapiens
Manually annotated by BRENDA team
Holmes, E.H.; Yen, T.Y.; Thomas, S.; Joshi, R.; Nguyen, A.; Long, T.; Gallet, F.; Maftah, A.; Julien, R.; Macher, B.A.
Human alpha1,3/4 fucosyltransferases. Characterization of highly conserved cysteine residues and N-linked glycosylation sites
J. Biol. Chem.
275
24237-24245
2000
Homo sapiens
Manually annotated by BRENDA team
Dupuy, F.; Germot, A.; Marenda, M.; Oriol, R.; Blancher, A.; Julien, R.; Maftah, A.
alpha1,4-Fucosyltransferase activity: a significant function in the primate lineage has appeared twice independently
Mol. Biol. Evol.
19
815-824
2002
Homo sapiens, Macaca mulatta
Manually annotated by BRENDA team
Morais, V.A.; Serpa, J.; Palma, A.S.; Costa, T.; Maranga, L.; Costa, J.
Expression and characterization of recombinant human alpha-3/4-fucosyltransferase III from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) cells using the baculovirus expression system
Biochem. J.
353
719-725
2001
Homo sapiens
Manually annotated by BRENDA team
Weston, B.W.; Nair, R.P.; Larsen, R.D.; Lowe, J.B.
Isolation of a novel human alpha (1,3)fucosyltransferase gene and molecular comparison to the human Lewis blood group alpha (1,3/1,4)fucosyltransferase gene. Syntenic, homologous, nonallelic genes encoding enzymes with distinct acceptor substrate specificities
J. Biol. Chem.
267
4152-4160
1992
Homo sapiens
Manually annotated by BRENDA team
Vo, L.; Lee, S.; Marcinko, M.C.; Holmes, E.H.; Macher, B.A.
Human alpha1,3/4-fucosyltransferases II. A single amino acid at the COOH terminus of FucT III and V alters their kinetic properties
J. Biol. Chem.
273
25250-25255
1998
Homo sapiens
Manually annotated by BRENDA team
Dupuy, F.; Petit, J.M.; Mollicone, R.; Oriol, R.; Julien, R.; Maftah, A.
A single amino acid in the hypervariable stem domain of vertebrate alpha1,3/1,4-fucosyltransferases determines the type 1/type 2 transfer. Characterization of acceptor substrate specificity of the Lewis enzyme by site-directed mutagenesis
J. Biol. Chem.
274
12257-12262
1999
Homo sapiens
Manually annotated by BRENDA team
Chandrasekaran, E.V.; Chawda, R.; Rhodes, J.M.; Xia, J.; Piskorz, C.; Matta, K.L.
Human lung adenocarcinoma alpha1,3/4-L-fucosyltransferase displays two molecular forms, high substrate affinity for clustered sialyl LacNAc type 1 units as well as mucin core 2 sialyl LacNAc type 2 unit and novel alpha1,2-L-fucosylating activity
Glycobiology
11
353-363
2001
Homo sapiens
Manually annotated by BRENDA team
Costa, J.; Grabenhorst, E.; Nimtz, M.; Conradt, H.S.
Stable expression of the Golgi form and secretory variants of human fucosyltransferase III from BHK-21 cells. Purification and characterization of an engineered truncated form from the culture medium
J. Biol. Chem.
272
11613-11621
1997
Homo sapiens
Manually annotated by BRENDA team
Kukowska-Latallo, J.F.; Larsen, R.D.; Nair, R.P.; Lowe, J.B.
A cloned human cDNA determines expression of mouse stage-specific embryonic antigen and the Lewis blood group alpha(1,3/1,4)fucosyltransferase
Genes Dev.
4
1288-1303
1990
Homo sapiens (P21217)
Manually annotated by BRENDA team
Cameron, H.S.; Szczepaniak, D.; Weston, B.W.
Expression of human chromosome 19p alpha(1,3)-fucosyltransferase genes in normal tissues. Alternative splicing, polyadenylation, and isoforms
J. Biol. Chem.
270
20112-20122
1995
Homo sapiens (P21217)
Manually annotated by BRENDA team
Elmgren, A.; Rydberg, L.; Larson, G.
Genotypic heterogeneity among Lewis negative individuals
Biochem. Biophys. Res. Commun.
196
515-520
1993
Homo sapiens (P21217)
Manually annotated by BRENDA team
Nishihara, S.; Yazawa, S.; Iwasaki, H.; Nakazato, M.; Kudo, T.; Ando, T.; Narimatsu, H.
alpha(1,3/1,4)fucosyltransferase (FucT-III) gene is inactivated by a single amino acid substitution in Lewis histo-blood type negative individuals
Biochem. Biophys. Res. Commun.
196
624-631
1993
Homo sapiens, Homo sapiens (P21217)
Manually annotated by BRENDA team
Koda, Y.; Kimura, H.; Mekada, E.
Analysis of Lewis fucosyltransferase genes from the human gastric mucosa of Lewis-positive and -negative individuals
Blood
82
2915-2919
1993
Homo sapiens (P21217)
Manually annotated by BRENDA team
Mollicone, R.; Reguigne, I.; kelly, R.J.; Fletcher, A.; Watt, J.; Chatfield, S.; Aziz, A.; Cameron, H.S.; Weston, B.W.; Lowe, J.B.; Oriol, R.
Molecular basis for Lewis alpha(1,3/1,4)-fucosyltransferase gene deficiency (FUT3) found in Lewis-negative Indonesian pedigrees
J. Biol. Chem.
269
20987-20994
1994
Homo sapiens (P21217)
Manually annotated by BRENDA team
Nishihara, S.; Narimatsu, H.; Iwasaki, H.; Yazawa, S.; Akamatsu, S.; Ando, T.; Sena, T.; Narimatsu, I.
Molecular genetic analysis of the human Lewis histo-blood group system
J. Biol. Chem.
269
29271-29278
1994
Homo sapiens, Homo sapiens (P21217)
Manually annotated by BRENDA team
Elmgren, A.; Boerjeson, C.; Svensson, L.; Rydberg, L.; Larson, G.
DNA sequencing and screening for point mutations in the human Lewis ‚FUT‘ gene enables molecular genotyping of the human Lewis blood group system
Vox Sang.
70
97-103
1996
Homo sapiens (P21217)
Manually annotated by BRENDA team
Elmgren, A.; Mollicone, R.; Costache, M.; Boerjeson, C.; Oriol, R.; Harrington, J.; Larson, G.
Significance of individual point mutations, T202C and C314T in the human Lewis ‚FTA3' gene for expression of Lewis antigens by human alpha‘1,3/1,4'-fucosyltransferase, Fuc-TIII
J. Biol. Chem.
272
21994-21998
1997
Homo sapiens, Homo sapiens (P21217)
Manually annotated by BRENDA team
Pang, H.; Liu, Y.; Koda, Y.; Soejima, M.; Jia, J.; Schlaphoff, T.; du Toit, E.D.; Kimura, H.
Five novel missense mutations of the Lewis gene ‚FUT3' in African ‚Xhosa‘ and Caucasian populations in South Africa‘
Hum. Genet.
102
675-680
1998
Homo sapiens (P21217)
Manually annotated by BRENDA team
Koszdin, K.L.; Bowen, B.R.
The cloning and expression of a human alpha-1,3 fucosyltransferase capable of forming the E-selectin ligand
Biochem. Biophys. Res. Commun.
187
152-157
1992
Homo sapiens, Homo sapiens (P51993)
Manually annotated by BRENDA team
Galan, M.C.; Venot, A.P.; Boons, G.J.
Glycosyltransferase activity can be modulated by small conformational changes of acceptor substrates
Biochemistry
42
8522-8529
2003
Homo sapiens
Manually annotated by BRENDA team
Niu, X.; Fan, X.; Sun, J.; Ting, P.; Narula, S.; Lundell, D.
Inhibition of fucosyltransferase VII by gallic acid and its derivatives
Arch. Biochem. Biophys.
425
51-57
2004
Homo sapiens (Q11130)
Manually annotated by BRENDA team
Stoykova, L.I.; Liu, A.; Scanlin, T.F.; Glick, M.C.
alpha1,3 Fucosyltransferases in cystic fibrosis airway epithelial cells
Biochimie
85
363-367
2003
Homo sapiens
Manually annotated by BRENDA team
Palma, A.S.; Morais, V.A.; Coelho, A.V.; Costa, J.
Effect of the manganese ion on human alpha3/4 fucosyltransferase III activity
BioMetals
17
35-43
2004
Homo sapiens
Manually annotated by BRENDA team
Norris, A.J.; Whitelegge, J.P.; Strouse, M.J.; Faull, K.F.; Toyokuni, T.
Inhibition kinetics of carba- and C-fucosyl analogues of GDP-fucose against fucosyltransferase V: implication for the reaction mechanism
Bioorg. Med. Chem. Lett.
14
571-573
2004
Homo sapiens
Manually annotated by BRENDA team
Chandrasekaran, E.V.; Chawda, R.; Rhodes, J.M.; Locke, R.D.; Piskorz, C.F.; Matta, K.L.
The binding characteristics and utilization of Aleuria aurantia, Lens culinaris and few other lectins in the elucidation of fucosyltransferase activities resembling cloned FT VI and apparently unique to colon cancer cells
Carbohydr. Res.
338
887-901
2003
Homo sapiens (P51993), Homo sapiens (Q11128)
Manually annotated by BRENDA team
Abe, H.; Ohba, M.; Shimma, Y.I.; Jigami, Y.
Yeast cells harboring human alpha-1,3-fucosyltransferase at the cell surface engineered using Pir, a cell wall-anchored protein
FEMS Yeast Res.
4
417-425
2004
Homo sapiens
Manually annotated by BRENDA team
Dupuy, F.; Germot, A.; Julien, R.; Maftah, A.
Structure/function study of Lewis alpha3- and alpha3/4-fucosyltransferases: the alpha1,4 fucosylation requires an aromatic residue in the acceptor-binding domain
Glycobiology
14
347-356
2004
Gorilla gorilla (Q8HYJ6), Gorilla gorilla (Q8HYJ7), Homo sapiens (P21217), Homo sapiens (P51993), Homo sapiens (Q11128), Hylobates lar (Q8HYJ3), Pongo pygmaeus (Q8HYJ4), Pongo pygmaeus (Q8HYJ5)
Manually annotated by BRENDA team
Morais, V.A.; Costa, J.
Stable expression of recombinant human alpha3/4 fucosyltransferase III in Spodoptera frugiperda Sf9 cells
J. Biotechnol.
106
69-75
2003
Homo sapiens
Manually annotated by BRENDA team
Guo, P.; Zhang, Y.; Zhang, X.Y.; Chen, H.L.; Wang, H.; Narimatsu, H.
Analysis of lewis antigens on cell surface and alpha1,3 fucosyltransferase subtypes in H7721 human hepatocarcinoma cells
J. Exp. Clin. Cancer Res.
22
135-139
2003
Homo sapiens
Manually annotated by BRENDA team
Bengtson, P.; Lundblad, A.; Larson, G.; Pahlsson, P.
Polymorphonuclear leukocytes from individuals carrying the G329A mutation in the alpha 1,3-fucosyltransferase VII gene (FUT7) roll on E- and P-selectins
J. Immunol.
169
3940-3946
2002
Homo sapiens
Manually annotated by BRENDA team
Dabrowska, A.; Baczynska, D.; Widerak, K.; Laskowska, A.; Ugorski, M.
Promoter analysis of the human alpha1,3/4-fucosyltransferase gene (FUT III)
Biochim. Biophys. Acta
1731
66-73
2005
Homo sapiens
Manually annotated by BRENDA team
Escrevente, C.; Machado, E.; Brito, C.; Reis, C.A.; Stoeck, A.; Runz, S.; Marme, A.; Altevogt, P.; Costa, J.
Different expression levels of alpha3/4 fucosyltransferases and Lewis determinants in ovarian carcinoma tissues and cell lines
Int. J. Oncol.
29
557-566
2006
Homo sapiens
Manually annotated by BRENDA team
Khaled, A.; Piotrowska, O.; Dominiak, K.; Auge, C.
Exploring specificity of glycosyltransferases: synthesis of new sugar nucleotide related molecules as putative donor substrates
Carbohydr. Res.
343
167-178
2008
Homo sapiens
Manually annotated by BRENDA team
Shetterly, S.; Jost, F.; Watson, S.R.; Knegtel, R.; Macher, B.A.; Holmes, E.H.
Site-specific fucosylation of sialylated polylactosamines by alpha1,3/4-fucosyltransferases-V and -VI is defined by amino acids near the N terminus of the catalytic domain
J. Biol. Chem.
282
24882-24892
2007
Homo sapiens
Manually annotated by BRENDA team
Lin, C.H.; Lin, C.W.; Khoo, K.H.
Proteomic identification of specific glycosyltransferases functionally implicated for the biosynthesis of a targeted glyco-epitope
Proteomics
8
475-483
2008
Homo sapiens (P21217), Homo sapiens (P51993)
Manually annotated by BRENDA team
Tefsen, B.; van Stijn, C.M.; van den Broek, M.; Kalay, H.; Knol, J.C.; Jimenez, C.R.; van Die, I.
Chemoenzymatic synthesis of multivalent neoglycoconjugates carrying the helminth glycan antigen LDNF
Carbohydr. Res.
344
1501-1507
2009
Homo sapiens
Manually annotated by BRENDA team
Fagerberg, D.; Angstroem, J.; Halim, A.; Hultberg, A.; Rakhimova, L.; Hammarstroem, L.; Boren, T.; Teneberg, S.
Novel Leb-like Helicobacter pylori-binding glycosphingolipid created by the expression of human alpha-1,3/4-fucosyltransferase in FVB/N mouse stomach
Glycobiology
19
182-191
2009
Homo sapiens
Manually annotated by BRENDA team
Carvalho, A.; Harduin-Lepers, A.; Magalhaes, A.; Machado, E.; Mendes, N.; Costa, L.; Matthiesen, R.; Almeida, R.; Costa, J.; Reis, C.
Differential expression of alpha-2,3-sialyltransferases and alpha-1,3/4-fucosyltransferases regulates the levels of sialyl Lewis a and sialyl Lewis x in gastrointestinal carcinoma cells
Int. J. Biochem. Cell Biol.
42
80-89
2010
Homo sapiens
Manually annotated by BRENDA team
Mollicone, R.; Moore, S.E.; Bovin, N.; Garcia-Rosasco, M.; Candelier, J.J.; Martinez-Duncker, I.; Oriol, R.
Activity, splice variants, conserved peptide motifs, and phylogeny of two new alpha1,3-fucosyltransferase families (FUT10 and FUT11)
J. Biol. Chem.
284
4723-4738
2009
Homo sapiens (Q495W5), Homo sapiens (Q6P4F1)
Manually annotated by BRENDA team
Barthel, S.; Wiese, G.; Cho, J.; Opperman, M.; Hays, D.; Siddiqui, J.; Pienta, K.; Furie, B.; Dimitroff, C.
Alpha 1,3 fucosyltransferases are master regulators of prostate cancer cell trafficking
Proc. Natl. Acad. Sci. USA
106
19491-19496
2009
Homo sapiens
Manually annotated by BRENDA team
Guo, Q.; Guo, B.; Wang, Y.; Wu, J.; Jiang, W.; Zhao, S.; Qiao, S.; Wu, Y.
Functional analysis of alpha1,3/4-fucosyltransferase VI in human hepatocellular carcinoma cells
Biochem. Biophys. Res. Commun.
417
311-317
2012
Homo sapiens
Manually annotated by BRENDA team
Seelhorst, K.; Piernitzki, T.; Lunau, N.; Meier, C.; Hahn, U.
Synthesis and analysis of potential alpha1,3-fucosyltransferase inhibitors
Bioorg. Med. Chem.
22
6430-6437
2014
Helicobacter pylori, Homo sapiens
Manually annotated by BRENDA team
Lunau, N.; Seelhorst, K.; Kahl, S.; Tscherch, K.; Stacke, C.; Rohn, S.; Thiem, J.; Hahn, U.; Meier, C.
Fluorescently labeled substrates for monitoring alpha1,3-fucosyltransferase IX activity
Chemistry
19
17379-17390
2013
Helicobacter pylori, Homo sapiens
Manually annotated by BRENDA team
Brazil, J.C.; Liu, R.; Sumagin, R.; Kolegraff, K.N.; Nusrat, A.; Cummings, R.D.; Parkos, C.A.; Louis, N.A.
alpha3/4 Fucosyltransferase 3-dependent synthesis of Sialyl Lewis A on CD44 variant containing exon 6 mediates polymorphonuclear leukocyte detachment from intestinal epithelium during transepithelial migration
J. Immunol.
191
4804-4817
2013
Homo sapiens
Manually annotated by BRENDA team
Huang, V.W.; Lee, C.L.; Lee, Y.L.; Lam, K.K.; Ko, J.K.; Yeung, W.S.; Ho, P.C.; Chiu, P.C.
Sperm fucosyltransferase-5 mediates spermatozoa-oviductal epithelial cell interaction to protect human spermatozoa from oxidative damage
Mol. Hum. Reprod.
21
516-526
2015
Homo sapiens
Manually annotated by BRENDA team
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