Protein Variants | Comment | Organism |
---|---|---|
D112E | muation decreases activity of the enzyme and does not interfere with H-type 1/H-type 2 acceptors | Homo sapiens |
W111R | the mutation changes the specificity for fucose transfer from H-type 1 to H-type 2 acceptors | Homo sapiens |
W11R/D112E | the mutation changes the specificity for fucose transfer from H-type 1 to H-type 2 acceptors. Increased type 2 activity compared to mutant W111R | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
GDP-L-fucose | mutant enzyme W111R | Homo sapiens | |
0.032 | - |
GDP-L-fucose | mutant enzyme W111R/D112E | Homo sapiens | |
0.033 | - |
GDP-L-fucose | mutant enzyme D112E | Homo sapiens | |
0.035 | - |
GDP-L-fucose | wild-type enzyme | Homo sapiens | |
0.2 | - |
Fucalpha(1,2)Galbeta(1,3)GlcNAc | wild-type enzyme | Homo sapiens | |
0.4 | - |
Fucalpha(1,2)Galbeta(1,3)GlcNAc | mutant enzyme D112E | Homo sapiens | |
0.5 | - |
Fucalpha(1,2)Galbeta(1,4)GlcNAc | mutant enzyme W111R/D112E | Homo sapiens | |
0.7 | - |
Fucalpha(1,2)Galbeta(1,4)GlcNAc | mutant enzyme W111R | Homo sapiens | |
2 | - |
Fucalpha(1,2)Galbeta(1,3)GlcNAc | mutant enzyme W111R/D112E | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
fucosyltransferase III | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc | activity of the wild-type enzyme is about 3% of the activity with Fucalpha(1,2)Galbeta(1,4)GlcNAc. The mutation W111R shows higher activity for Fucalpha(1,2)Galbeta(1,3)GlcNAc than for Fucalpha(1,2)Galbeta(1,4)GlcNAc. The addition mutation in W11R/D112E increases activity for Fucalpha(1,2)Galbeta(1,3)GlcNAc compared to mutant W111R | Homo sapiens | GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAc | - |
? | |
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc | - |
Homo sapiens | GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc | - |
? |