Application | Comment | Organism |
---|---|---|
synthesis | the soluble form of fucosyltransferase III secreted by stably transfected cells may be used for in vitro synthesis of the Lewis 1 determinant on carbohydrates and glycoproteins | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
construction of plasmids encoding soluble forms of the recombinant human FucT-III where the human IL-2 sequence is linked to Ala47 or Val-36 of the FucT-III and expression in stable transfected BHK-21 cell lines | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | stable BHK-21 cell lines express the Golgi bound form and two secretory forms of the enzyme | Homo sapiens | 16020 | - |
soluble | stable BHK-21 cell lines express the Golgi bound form and two secretory forms of the enzyme. 40% of the enzyme activity synthesized by cells transfected with the Golgi form of the enzyme are constitutively secreted into the medium | Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the secretory variant of enzyme contains N-linked endo H sensitive carbohydrate chains at its two glycosylation sites | Homo sapiens |
Purification (Comment) | Organism |
---|---|
secretory variant of enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + asialofetuin | the acceptor oligosaccharide in bovine asialofetuin is the man3 branched triantennary isomer with one Galbeta(1,3)GlcNAc | Homo sapiens | GDP + ? | - |
? | |
GDP-L-fucose + IL-4 receptor | - |
Homo sapiens | GDP + ? | - |
? | |
additional information | the enzyme transferrs fucose to the O-4-position of GlcNAc in small oligosaccharides, glycolipids, glycopeptides and glycoproteins containing the type I Galbeta(1,3)GlcNAc motif | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000-42000, SDS-PAGE | Homo sapiens |