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Literature summary for 2.4.1.65 extracted from
- Yeast cells harboring human alpha-1,3-fucosyltransferase at the cell surface engineered using Pir, a cell wall-anchored protein (2004), FEMS Yeast Res., 4, 417-425.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | by constructing yeast cells that display human FUT6 on the cell wall by fusion of FUT6 with the yeast cell wall proteins Pir1 and Pir2, the fucosylated oligosaccharides can be easily synthesized by the incubation of yeast cells with an appropriate donor and acceptor. It will thus be possible to prepare a large amount of immobilized FUT6 fused with Pir proteins in an inexpensive medium lacking the serum that is required for mammalian cell cultivation | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Saccharomyces cerevisiae, construction of fusion genes PIR1-HA-FUT6 and PIR2-FLAG-FUT6 | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | fusion proteins PIR1-HA-FUT6 and PIR2-FLAG-FUT6 | Homo sapiens | 9986 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-fucose + LacNAc | - |
Homo sapiens | ? | - |
? |  |
| pyridylamine-lacto-N-neotetraose + GDP-fucose | - |
Homo sapiens | GDP + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-1,3-fucosyltransferase | - |
Homo sapiens |
| FucT | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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