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Information on EC 1.12.1.2 - hydrogen dehydrogenase
for references in articles please use BRENDA:EC1.12.1.2
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EC Tree 1 Oxidoreductases
1.12 Acting on hydrogen as donor
1.12.1 With NAD+ or NADP+ as acceptor
1.12.1.2 hydrogen dehydrogenase
IUBMB Comments
An iron-sulfur flavoprotein (FMN or FAD). Some forms of this enzyme contain nickel.
The enzyme appears in viruses and cellular organisms
- 1.12.1.2
- alcaligenes
- eutrophus
- hydrogenases
-
ralstonia
-
hydrogen-oxidizing
-
h2-dependent
-
lithoautotrophic
-
thiocapsa
- roseopersicina
-
h2-oxidizing
-
oxygen-tolerant
- synthesis
showSynonyms
soluble hydrogenase, hoxefuyh, hox hydrogenase, nad-dependent hydrogenase, nad-reducing hydrogenase, soluble [nife]-hydrogenase, bidirectional [nife] hydrogenase, fehyd, iron-hydrogenase, nad+-reducing hydrogenase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
dehydrogenase, hydrogen
-
-
-
-
FeHyd
H2:NAD+ oxidoreductase
-
-
-
-
H2ase
Hox
Hox hydrogenase
Hox type hydrogenase
HoxE
HoxEFUYH
HoxF
HoxFUYHI2
HoxH
HoxS
HoxU
HoxY
hydrogenase
-
-
-
-
Hyn
iron-hydrogenase
NAD+-reducing [NiFe]-hydrogenase
NAD-dependent hydrogenase
SH
soluble [NiFe]-hydrogenase
[FeFe] hydrogenase
[FeFe]-hydrogenase
[NiFe] hydrogenase
[NiFe]-hydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
H2 + NAD+ = H+ + NADH
catalytic activity may be regulated by the reduction of a regulatory center followed by a series of structural changes
-
H2 + NAD+ = H+ + NADH
catalytic activity may be regulated by the reduction of a regulatory center followed by a series of structural changes
-
-
H2 + NAD+ = H+ + NADH
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
MetaCyc
hydrogen oxidation II (aerobic, NAD), hydrogen production II
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SYSTEMATIC NAME
IUBMB Comments
hydrogen:NAD+ oxidoreductase
An iron-sulfur flavoprotein (FMN or FAD). Some forms of this enzyme contain nickel.
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CAS REGISTRY NUMBER
COMMENTARY
37256-54-5
deleted
9027-05-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ferrocyanide + NAD(P)H
ferricyanide + NAD(P)+
-
Substrates: enzyme complex including HoxI
Products: -
Products: -
?
H+ + reduced methyl viologen
H2 + methyl viologen
-
Substrates: -
Products: -
Products: -
?
H2 + oxidized dithionite
H+ + reduced dithionite
Substrates: -
Products: -
Products: -
r
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
NAD(P)+ + reduced 2,6-dichlorophenolindophenol
NADH + oxidized 2,6-dichlorophenol indophenol
NAD+ + reduced 2,6-dichlorophenol indophenol
NADH + oxidized benzyl viologen
NAD+ + reduced benzyl viologen
-
Substrates: -
Products: -
Products: -
r
NADH + reduced 3-acetylpyridine adenine dinucleotide
NAD+ + oxidized 3-acetylpyridine adenine dinucleotide
NADPH + K3Fe(CN)6
?
-
Substrates: low reaction with hexameric enzyme form, no reaction with tetrameric enzyme form
Products: -
Products: -
?
oxidized 2,6-dichlorophenol indophenol + NADH + H+
reduced 2,6-dichlorophenolindophenol + NAD+
oxidized cytochrome c + NAD(P)H
reduced cytchrome c + NAD(P)+
-
Substrates: -
Products: -
Products: -
?
oxidized methylene blue + H2
reduced methylene blue + H+
-
Substrates: 1058% activity compared to NAD+
Products: -
Products: -
r
oxidized methylene blue + NAD(P)H
reduced methylene blue + NAD(P)+
-
Substrates: -
Products: -
Products: -
?
2 ferricyanide + NAD(P)H
2 ferrocyanide + NAD(P)+ + H+
-
Substrates: -
Products: -
Products: -
?
2 ferricyanide + NAD(P)H
2 ferrocyanide + NAD(P)+ + H+
-
Substrates: -
Products: -
Products: -
?
2 ferricyanide + NAD(P)H
2 ferrocyanide + NAD(P)+ + H+
-
Substrates: -
Products: -
Products: -
?
2 ferricyanide + NAD(P)H
2 ferrocyanide + NAD(P)+ + H+
-
Substrates: -
Products: -
Products: -
?
2 ferricyanide + NAD(P)H
2 ferrocyanide + NAD(P)+ + H+
-
Substrates: -
Products: -
Products: -
?
2 ferricyanide + NAD(P)H
2 ferrocyanide + NAD(P)+ + H+
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: -
Products: -
Products: -
?
2 ferricyanide + NAD(P)H
2 ferrocyanide + NAD(P)+ + H+
-
Substrates: -
Products: -
Products: -
?
ferrocyanide + NAD+
ferricyanide + NADH
-
Substrates: -
Products: -
Products: -
r
ferrocyanide + NAD+
ferricyanide + NADH
-
Substrates: diaphorase activity
Products: -
Products: -
r
ferrocyanide + NAD+
ferricyanide + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: -
Products: -
Products: -
r
H+ + NADH
H2 + NAD+
Substrates: module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple
Products: -
Products: -
r
H+ + NADH
H2 + NAD+
Substrates: module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple
Products: -
Products: -
r
H+ + NADH + reduced ferredoxin
H2 + NAD+ + oxidized ferredoxin
-
Substrates: the enzyme simultaneously uses electrons from NADH and reduced ferredoxin to produce hydrogen
Products: -
Products: -
?
H+ + NADH + reduced ferredoxin
H2 + NAD+ + oxidized ferredoxin
-
Substrates: the enzyme simultaneously uses electrons from NADH and reduced ferredoxin to produce hydrogen
Products: -
Products: -
?
H+ + NADH + reduced ferredoxin
H2 + NAD+ + oxidized ferredoxin
-
Substrates: bifurcating [FeFe]-hydrogenase in Syntrophobacter fumaroxidans simultaneously uses electrons from NADH and reduced ferredoxin in a 1:2 ratio to produce hydrogen
Products: -
Products: -
?
H+ + NADH + reduced ferredoxin
H2 + NAD+ + oxidized ferredoxin
-
Substrates: bifurcating [FeFe]-hydrogenase in Syntrophobacter fumaroxidans simultaneously uses electrons from NADH and reduced ferredoxin in a 1:2 ratio to produce hydrogen
Products: -
Products: -
?
H+ + NADH + reduced ferredoxin
H2 + NAD+ + oxidized ferredoxin
-
Substrates: the hydrogenase requires the presence of both electron carriers, NADH and reduced ferredoxin, synergistically in a 1:1 ratio for catalysis of H2 production
Products: -
Products: -
?
H+ + NADH + reduced ferredoxin
H2 + NAD+ + oxidized ferredoxin
-
Substrates: the hydrogenase requires the presence of both electron carriers, NADH and reduced ferredoxin, synergistically in a 1:1 ratio for catalysis of H2 production
Products: -
Products: -
?
H+ + reduced dithionite
H2 + oxidized dithionite
-
Substrates: -
Products: -
Products: -
?
H+ + reduced dithionite
H2 + oxidized dithionite
-
Substrates: -
Products: -
Products: -
?
H+ + reduced dithionite
H2 + oxidized dithionite
-
Substrates: -
Products: -
Products: -
?
H+ + reduced methyl viologen
H2 + oxidized methyl viologen
-
Substrates: -
Products: -
Products: -
?
H+ + reduced methyl viologen
H2 + oxidized methyl viologen
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: -
Products: -
Products: -
?
H+ + reduced methyl viologen
H2 + oxidized methyl viologen
-
Substrates: -
Products: -
Products: -
?
H+ + reduced methyl viologen
H2 + oxidized methyl viologen
-
Substrates: -
Products: -
Products: -
?
H+ + reduced methyl viologen
H2 + oxidized methyl viologen
-
Substrates: -
Products: -
Products: -
?
H2
H+ + e-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: hydrogenase reaction part
Products: -
Products: -
r
H2 + acceptor
H+ + reduced acceptor
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: -
Products: -
Products: -
?
H2 + ferricyanide
H+ + ferrocyanide
-
Substrates: 1257% activity compared to electron acceptor NAD+
Products: -
Products: -
?
H2 + ferricyanide
H+ + ferrocyanide
-
Substrates: 585% activity compared to electron acceptor NAD+
Products: -
Products: -
?
H2 + ferricyanide
H+ + ferrocyanide
-
Substrates: 1257% activity compared to electron acceptor NAD+
Products: -
Products: -
?
H2 + ferricyanide
H+ + ferrocyanide
-
Substrates: 389% activity compared to electron acceptor NAD+
Products: -
Products: -
?
H2 + ferricyanide
H+ + ferrocyanide
-
Substrates: 708% activity compared to electron acceptor NAD+
Products: -
Products: -
?
H2 + ferricyanide
H+ + ferrocyanide
-
Substrates: 708% activity compared to electron acceptor NAD+
Products: -
Products: -
?
H2 + ferricyanide
H+ + ferrocyanide
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: 708% activity compared to electron acceptor NAD+
Products: -
Products: -
?
H2 + ferricyanide
H+ + ferrocyanide
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: -
Products: -
Products: -
r
H2 + ferricyanide
H+ + ferrocyanide
-
Substrates: 350% activity compared to electron acceptor NAD+
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: -
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: electron transfer between the catalytic site for NADH-oxidation and the hydrogenase catalytic site is rate-limiting
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
439633, 439692, 439694, 439695, 439696, 439697, 439700, 439704, 439705, 439706, 439707, 439709, 439710, 439711, 439712, 656298, 672067, 675493, 726344
Substrates: -
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptors: Janus green, 2,6-dichlorophenolindophenol, phenazine methosulfate, menaquinone, ubiquinone, cytochrome c
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: hydrogenase produces superoxide free radical anions, which are responsible for enzyme inactivation
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor benzyl viologen
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor methyl viologen
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: weak electron acceptor O2
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: enzyme also has diaphorase and NAD(P)H oxidase activity
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor ferricyanide
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor resazurin
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor methylene blue
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: utilization of H2 as energy source during autotrophic growth on hydrogen and CO2
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: enzyme provides reducing equivalents for CO2 fixation
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: key enzyme in H2 metabolism
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: H2 activation solely takes place on Ni2+
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: the enzyme catalyzes electron transfer from molecular hydrogen to NAD+, thereby producing reducing equivalents for CO2 fixation in the form of NADH
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
Substrates: module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
Substrates: module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: -
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: utilization of H2 as energy source during autotrophic growth on hydrogen and CO2
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: -
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: electron acceptor FMN
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: electron acceptor FAD
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: artificial electron acceptors: Janus green, 2,6-dichlorophenolindophenol, phenazine methosulfate, menaquinone, ubiquinone, cytochrome c
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: -
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: hydrogenase produces superoxide free radical anions, which are responsible for enzyme inactivation
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: -
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: -
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: no activity with NADP+
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: key enzyme in H2 metabolism
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: -
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Substrates: overall reaction
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor resazurin
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: physiological function of the enzyme in Frankia strains, overview
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
Substrates: -
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
Substrates: H2 producing activity is not dependent on NAD+ reduction
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: only 3-8% of the benzyl biologen or methyl viologen-dependent values. The enzyme interacts with NADP+ and NADPH, but is more specific to NAD+ and NADH
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: only 3-8% of the benzyl biologen or methyl viologen-dependent values. The enzyme interacts with NADP+ and NADPH, but is more specific to NAD+ and NADH
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
Hydrogenomonas sp.
-
Substrates: electron acceptor FAD, only in presence of catalytic amounts of NAD+
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
Hydrogenomonas sp.
-
Substrates: artificial electron acceptor methylene blue, only in presence of catalytic amounts of NAD+
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
Hydrogenomonas sp.
-
Substrates: electron acceptor FMN, only in presence of catalytic amounts of NAD+
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: electron acceptor FAD, only in presence of catalytic amounts of NAD+
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor methylene blue, only in presence of catalytic amounts of NAD+
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: electron acceptor FMN, only in presence of catalytic amounts of NAD+
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: enzyme delivers hydrogen-driven reducing power for methane oxidation
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor benzyl viologen
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor benzyl viologen
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor methyl viologen
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor methyl viologen
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor ferricyanide
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor methylene blue, only in presence of catalytic amounts of NAD+
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor methylene blue
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor methylene blue
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor benzyl viologen
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor methyl viologen
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor methylene blue, only in presence of catalytic amounts of NAD+
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor methylene blue
Products: -
Products: -
?
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor benzyl viologen
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
-
Substrates: artificial electron acceptor methyl viologen
Products: -
Products: -
r
H2 + NAD+
H+ + NADH
Substrates: in vivo H2 evolution under nitrogenase-repressed conditions
Products: -
Products: -
r
H2 + NADP+
H+ + NADPH
-
Substrates: only 3-8% of the benzyl biologen or methyl viologen-dependent values. The enzyme interacts with NADP+ and NADPH, but is more specific to NAD+ and NADH
Products: -
Products: -
r
H2 + NADP+
H+ + NADPH
-
Substrates: only 3-8% of the benzyl biologen or methyl viologen-dependent values. The enzyme interacts with NADP+ and NADPH, but is more specific to NAD+ and NADH
Products: -
Products: -
r
H2 + oxidized benzyl viologen
H+ + reduced benzyl viologen
-
Substrates: -
Products: -
Products: -
?
H2 + oxidized benzyl viologen
H+ + reduced benzyl viologen
-
Substrates: -
Products: -
Products: -
r
H2 + oxidized benzyl viologen
H+ + reduced benzyl viologen
-
Substrates: hydrogenase activity
Products: -
Products: -
r
H2 + oxidized benzyl viologen
H+ + reduced benzyl viologen
-
Substrates: -
Products: -
Products: -
r
H2 + oxidized benzyl viologen
H+ + reduced benzyl viologen
-
Substrates: -
Products: -
Products: -
r