Literature summary for 1.12.1.2 extracted from
Lauterbach, L.; Liu, J.; Horch, M.; Hummel, P.; Schwarze, A.; Haumann, M.; Vincent, K.; Lenz, O.; Zebger, I.
The hydrogenase subcomplex of the NAD+-reducing [NiFe] hydrogenase from Ralstonia eutropha - Insights into catalysis and redox interconversions (2011), Eur. J. Inorg. Chem., 2011, 1067-1079.
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
FMN |
H2 oxidation by subunits HoxHY is enhanced on addition of excess FMN |
Cupriavidus necator |
|
Cloned(Commentary)
Cloned (Comment) |
Organism |
expression in Cupriavidus necator |
Cupriavidus necator |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
O2 |
incubation of subunits HoxHY with O2 at high potentials causes slow inactivation, but activity is recovered within seconds at potentials below -170 mV at 30°C, even in the presence of 2% O2; incubation of subunits HoxHY with O2 at high potentials causes slow inactivation, but activity is recovered within seconds at potentials below -170 mV at 30°C, even in the presence of 2% O2 |
Cupriavidus necator |
|
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Iron |
the active site iron atom has a standard ligation, i.e., one CO and two cyanide ligands |
Cupriavidus necator |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Cupriavidus necator |
P22319 |
subunit delta |
- |
Cupriavidus necator |
P22320 |
subunit beta |
- |
Cupriavidus necator DSM 428 |
P22319 |
subunit delta |
- |
Cupriavidus necator DSM 428 |
P22320 |
subunit beta |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
recombinant protein. The as-isolated module HoxHY is initially catalytically inactive, but after reductive activation at low potentials, exhibits both H2 oxidation and H+ reduction |
Cupriavidus necator |
Synonyms
Synonyms |
Comment |
Organism |
HoxH |
- |
Cupriavidus necator |
HoxS |
- |
Cupriavidus necator |
Cofactor
Cofactor |
Comment |
Organism |
Structure |
4Fe-4S-center |
presence of a 4Fe-4S-center in addition to the active site iron |
Cupriavidus necator |
|
FMN |
cofactor and stabilization of the active site |
Cupriavidus necator |
|