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Literature summary for 1.12.1.2 extracted from

  • Lauterbach, L.; Liu, J.; Horch, M.; Hummel, P.; Schwarze, A.; Haumann, M.; Vincent, K.; Lenz, O.; Zebger, I.
    The hydrogenase subcomplex of the NAD+-reducing [NiFe] hydrogenase from Ralstonia eutropha - Insights into catalysis and redox interconversions (2011), Eur. J. Inorg. Chem., 2011, 1067-1079.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
FMN H2 oxidation by subunits HoxHY is enhanced on addition of excess FMN Cupriavidus necator

Cloned(Commentary)

Cloned (Comment) Organism
expression in Cupriavidus necator Cupriavidus necator

Inhibitors

Inhibitors Comment Organism Structure
O2 incubation of subunits HoxHY with O2 at high potentials causes slow inactivation, but activity is recovered within seconds at potentials below -170 mV at 30°C, even in the presence of 2% O2; incubation of subunits HoxHY with O2 at high potentials causes slow inactivation, but activity is recovered within seconds at potentials below -170 mV at 30°C, even in the presence of 2% O2 Cupriavidus necator

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the active site iron atom has a standard ligation, i.e., one CO and two cyanide ligands Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator P22319 subunit delta
-
Cupriavidus necator P22320 subunit beta
-
Cupriavidus necator DSM 428 P22319 subunit delta
-
Cupriavidus necator DSM 428 P22320 subunit beta
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein. The as-isolated module HoxHY is initially catalytically inactive, but after reductive activation at low potentials, exhibits both H2 oxidation and H+ reduction Cupriavidus necator

Synonyms

Synonyms Comment Organism
HoxH
-
Cupriavidus necator
HoxS
-
Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
4Fe-4S-center presence of a 4Fe-4S-center in addition to the active site iron Cupriavidus necator
FMN cofactor and stabilization of the active site Cupriavidus necator