Cloned (Comment) | Organism |
---|---|
overexpression of HoxE in Escherichia coli strain M15 | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.089 | - |
reduced methyl viologen | pH 6.3, 60°C | Synechocystis sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metalloenzyme | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
x * 18000, HoxE, SDS-PAGE, x * 21000, recombinant HIs-tagged HoxE, SDS-PAGE | Synechocystis sp. |
21000 | - |
x * 18000, HoxE, SDS-PAGE, x * 21000, recombinant HIs-tagged HoxE, SDS-PAGE | Synechocystis sp. |
375000 | - |
native dimeric assembly of the pentameric enzyme complex, gel filtration | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + NAD+ | Synechocystis sp. | - |
H+ + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | Q9Z354 | HoxE subunit from strain PCC 6301; strain PCC 6803, and strain PCC 6301 which is identical with Anacystis nidulans SAUG 1402-1 | - |
Oxidation Stability | Organism |
---|---|
enzyme is oxygen-labile | Synechocystis sp. |
Purification (Comment) | Organism |
---|---|
under anaerobic conditions, pentameric bidirectional hydrogenase complex HoxEFUYH to near homogeneity 647fold from strain PCC 6803 and 1290fold from strain PCC 6301, recombinant His-tagged HoxE from Escherichia coli | Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15 | - |
native purified enzyme complex from strain PCC 6301, hydrogen-forming activity, cofactor reduced methyl viologen | Synechocystis sp. |
67 | - |
native purified enzyme complex from strain PCC 6803, hydrogen-forming activity, cofactor reduced methyl viologen | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + NAD(P)+ | - |
Synechocystis sp. | H+ + NAD(P)H | - |
r | |
H2 + NAD+ | - |
Synechocystis sp. | H+ + NADH | - |
r | |
H2 + oxidized dithionite | - |
Synechocystis sp. | H+ + reduced dithionite | - |
r | |
H2 + oxidized methyl viologen | - |
Synechocystis sp. | H+ + reduced methyl viologen | best cofactor | r |
Subunits | Comment | Organism |
---|---|---|
? | x * 18000, HoxE, SDS-PAGE, x * 21000, recombinant HIs-tagged HoxE, SDS-PAGE | Synechocystis sp. |
More | the subunit HoxE, which is essential for the bidirectional hydrogenase activity, is included in a pentameric bidirectional hydrogenase complex HoxEFUYH in cyanobacteria, the complex forms dimer (HoxEFUYH)2, complex components are HoxE, HoxF, HoxH, HoxU, and HoxY, complex composition analysis, overview | Synechocystis sp. |
Synonyms | Comment | Organism |
---|---|---|
HoxE | - |
Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
strain PCC 6803, hydrogen-forming activity | Synechocystis sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
inactivation above | Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
strain PCC 6803, hydrogen-forming activity | Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | methyl viologen or dithionite can act as electron donor/acceptor | Synechocystis sp. | |
additional information | no activity with ferredoxin or FMN | Synechocystis sp. | |
NAD(P)+ | - |
Synechocystis sp. | |
NAD(P)H | NADH is 2times more active than NADPH | Synechocystis sp. |