Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O2 | slight inhibition of NAD+ reduction and H2 evolution from NADH | Cupriavidus necator |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Cupriavidus necator | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Cupriavidus necator | - |
formerly Alcaligenes eutrophus | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxidation with O2 and ferricyanide in absence of reducing components stabilizes, simultaneous presence of H2, NADH and O2 causes irreversible inactivation, half-lives in H2/O2 mixtures: 5% O2, 60 min, 20% O2, 30 min, 50% O2, 12 min | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + NAD+ | hydrogenase produces superoxide free radical anions, which are responsible for enzyme inactivation | Cupriavidus necator | H+ + NADH | - |
? | |
H2 + NAD+ | hydrogenase produces superoxide free radical anions, which are responsible for enzyme inactivation | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | H+ + NADH | - |
? |