Cloned (Comment) | Organism |
---|---|
- |
Cupriavidus necator |
Protein Variants | Comment | Organism |
---|---|---|
C461A | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive | Cupriavidus necator |
D456N | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive | Cupriavidus necator |
D456S | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive | Cupriavidus necator |
E14Q | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive | Cupriavidus necator |
E14V | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive | Cupriavidus necator |
G15A | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive | Cupriavidus necator |
H16L | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
H396L | site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
H396Q | site-directed mutagenesis, catalytic subunit HoxH allele, about 3.5fold reduced activity compared to the wild-type strain | Cupriavidus necator |
H69Q | site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain | Cupriavidus necator |
H69Q/P390A | site-directed mutagenesis, catalytic subunit HoxH alleles, reduced activity compared to the wild-type strain | Cupriavidus necator |
I64A | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
L118A | site-directed mutagenesis, catalytic subunit HoxH allele, reduced activity compared to the wild-type strain | Cupriavidus necator |
L118F | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow at O2 concentrations above 5%, about 4fold reduced activity compared to the wild-type strain | Cupriavidus necator |
L118I | site-directed mutagenesis, catalytic subunit HoxH allele, reduced activity compared to the wild-type strain | Cupriavidus necator |
L394A | site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain | Cupriavidus necator |
L394N | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive | Cupriavidus necator |
additional information | construction of an inactive null mutant strain, mutant strain growth characteristics, overview | Cupriavidus necator |
P390A | site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain | Cupriavidus necator |
R12L | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
R12Q | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
R391L | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive | Cupriavidus necator |
R391Q | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive | Cupriavidus necator |
R60Q | site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
S68V | site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
T415S | site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain | Cupriavidus necator |
T415V | site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
T415V/N415H | site-directed mutagenesis, catalytic subunit HoxH alleles, O2-sensitive growth, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Cupriavidus necator | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + NAD+ | Cupriavidus necator | - |
H+ + NADH | - |
? | |
additional information | Cupriavidus necator | activity is equally high in atmosperes of 20% O2, 20% N2, or 100% H2, the latter being slightly preferred | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
ATCC 17699, DSM 428 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
H2 + NAD+ = H+ + NADH | molecular mechanism of hydrogen activation | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + NAD+ | - |
Cupriavidus necator | H+ + NADH | - |
? | |
H2 + oxidized benzyl viologen | - |
Cupriavidus necator | H+ + reduced benzyl viologen | - |
? | |
additional information | activity is equally high in atmosperes of 20% O2, 20% N2, or 100% H2, the latter being slightly preferred | Cupriavidus necator | ? | - |
? | |
additional information | deuterium-hydrogen proton exchange activity of wild-type and mutantenzymes, overview | Cupriavidus necator | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | the catalytic subunit is termed HoxH | Cupriavidus necator |
Synonyms | Comment | Organism |
---|---|---|
NAD+-reducing hydrogenase | - |
Cupriavidus necator |
SH | - |
Cupriavidus necator |
soluble hydrogenase | - |
Cupriavidus necator |
[NiFe] hydrogenase | - |
Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
benzyl viologen | oxidized and reduced | Cupriavidus necator | |
NAD+ | - |
Cupriavidus necator | |
NADH | - |
Cupriavidus necator |