• BRENDA home

• Basic Ligand Information
• • Molecular Structure
  • Sum Formula
  • BRENDA Name
  • Synonyms
  • InChIKey
  • Pathways
• Roles as Enzyme Ligand
• • In Vivo Substrate
  • In Vivo Product
  • Substrate
  • Product
  • Cofactor
  • Activator
  • Inhibitor
  • Metals and Ions
  • 3D Structure (PDB)
• Enzyme Kinetic Parameters
• • k_cat Value (Turnover Number)
  • K_M Value
  • K_i Value
  • IC₅₀ Value
• References & Links
• • References
  • Links

print visible entries

print all entries

Ligand H2

Basic Ligand Information

Molecular Structure

Pathway Source

Pathways

BRENDA

coenzyme M biosynthesis, hydrogen production, methanogenesis from CO2, nitrate assimilation, sulfate reduction

KEGG

Carbohydrate digestion and absorption, Protein digestion and absorption, Two-component system

MetaCyc

(S)-lactate fermentation to propanoate, acetate and hydrogen, coenzyme B/coenzyme M regeneration I (methanophenazine-dependent), coenzyme B/coenzyme M regeneration IV (H2-dependent), hydrogen oxidation I (aerobic), hydrogen oxidation II (aerobic, NAD), hydrogen oxidation III (anaerobic, NADP), hydrogen production I, hydrogen production II, hydrogen production III, hydrogen production IV, hydrogen production V, hydrogen production VI, hydrogen production VIII, hydrogen to dimethyl sulfoxide electron transfer, hydrogen to fumarate electron transfer, hydrogen to trimethylamine N-oxide electron transfer, L-glutamate degradation VII (to butanoate), methanogenesis from H2 and CO2, mixed acid fermentation, nitrogen fixation I (ferredoxin), nitrogen fixation II (flavodoxin), reductive acetyl coenzyme A pathway II (autotrophic methanogens), sulfur reduction I, sulfur reduction II (via polysulfide), superpathway of fermentation (Chlamydomonas reinhardtii) more

Show all BRENDA pathways known for H2

close all tables

open all tables

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (24 results)

top print hide

Show Natural Substrate (24 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.12.1.2

H2 + NAD+ = H+ + NADH

439633, 656298, 742695, 742373, 742858, 743797, 656734, 654821, 742575, 439692, 439694, 654388, 654562, 655638, 654285, 655890, 655307, 672067

-

1.12.1.2

H2 + oxidized methyl viologen = H+ + reduced methyl viologen

655638

-

1.12.1.3

H2 + NADP+ = H+ + NADPH

704606, 439687, 687409

-

1.12.1.3

H2 + oxidized ferredoxin = H+ + reduced ferredoxin

439691

-

1.12.1.2

2 entries

1.12.1.3

2 entries

1.12.1.4

H2 + NAD+ + oxidized ferredoxin = H+ + NADH + reduced ferredoxin

741931

-

1.12.2.1

H2 + ferricytochrome c3 = H+ + ferrocytochrome c3

674919, 742006, 742696, 654899, 675360

-

1.12.7.2

H2 + oxidized ferredoxin = H+ + reduced ferredoxin

439643, 439665, 439672, 439632, 439669, 439671, 439675, 439679, 439682, 439677, 712301, 439654, 439650

-

1.12.7.2

H2 + oxidized ferredoxin = reduced ferredoxin + H+

660222, 742859, 742784, 742698, 659110

-

1.12.7.2

H2 + oxidized ferredoxin Pet F = reduced ferredoxin Pet F + H+

742279

-

1.12.98.1

H2 + coenzyme F420 = reduced coenzyme F420

659894, 246745, 395080, 395081, 395084, 395086, 395087, 395089, 395097, 395098, 395095, 395082, 395083, 395085, 395092, 395093, 395088, 395090, 395100, 395079, 395094, 687420

-

1.12.98.1

H2 + oxidized coenzyme F420 = reduced coenzyme F420

742368, 743086, 395099, 727399

-

1.12.98.1

H2 + oxidized methyl viologen = reduced methyl viologen

727399

-

1.12.7.2

3 entries

1.12.98.1

3 entries

1.12.98.2

5,10-methenyltetrahydromethanopterin + H2 = 5,10-methylenetetrahydromethanopterin + H+

657579, 658635, 658966, 658978, 696971

-

1.12.98.3

H2 + 2-(2,3-dihydropentaprenyloxy)phenazine = 2-dihydropentaprenyloxyphenazine

440299, 741956, 440300, 727829

-

1.12.99.6

H2 + acceptor = acceptor-H2

658987, 657739

-

1.12.99.6

H2 + acceptor = acceptorH2

658082, 659887

-

1.12.99.6

H2 + acceptor = H+ + reduced acceptor

713374, 712029, 439644, 439637, 439645, 439638, 439627, 439643, 439632, 671432

-

1.12.99.6

H2 + acceptor = reduced acceptor

742652

-

1.12.99.6

H2 + methylene blue = reduced methylene blue

742265

-

1.12.99.6

H2 + NADPH = NADP + H+

743732

-

1.12.99.6

6 entries

1.21.99.5

tetrachloroethene + 2 H2 = cis-1,2-dichloroethene + 2 chloride + 2 H+

657605

-

1.8.98.1

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 = coenzyme B + coenzyme M

655400, 655398, 655396, 655825

-

1.8.98.5

H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB = reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+

744833, 744179

-

1.97.1.9

selenate + H2 = selenite + H2O

658902

-

In Vivo Product in Enzyme-catalyzed Reactions (18 results)

top print hide

Show Natural Product (18 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.12.1.2

H+ + NADH = H2 + NAD+

-

-

1.12.1.2

H+ + NADPH = H2 + NADP+

-

-

1.12.1.2

2 entries

1.12.1.4

NADH + H+ + reduced ferredoxin = H2 + NAD+ + oxidized ferredoxin

-

-

1.12.7.2

2 H+ + reduced ferredoxin = H2 + oxidized ferredoxin

-

-

1.12.7.2

2 H+ + reduced polyferredoxin = H2 + oxidized polyferredoxin

-

-

1.12.7.2

H+ + reduced ferredoxin = H2 + oxidized ferredoxin

-

-

1.18.6.1

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

, 440174, 440188

-

1.18.6.1

N2 + 10 H+ + 8 e- + 16 ATP = 2 NH4+ + H2 + 16 ADP + 16 phosphate

-

-

1.18.6.1

N2 + 8 e- + 16 ATP + 8 H+ = 2 NH3 + H2 + 16 ADP + 16 phosphate

-

-

1.18.6.1

N2 + 8 e- + 8 H+ + 16 ATP = 2 NH3 + H2 + 16 ADP + 16 phosphate

-

-

1.18.6.1

reduced ferredoxin + H+ + N2 + ATP + H2O = oxidized ferredoxin + H2 + NH3 + ADP + phosphate

-

-

1.18.6.1

reduced ferredoxin + H+ + N2 + ATP = oxidized ferredoxin + H2 + NH3 + ADP + phosphate

-

-

1.12.7.2

3 entries

1.18.6.1

6 entries

1.18.6.2

12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O = 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate

-

-

1.19.6.1

reduced ferredoxin + H+ + N2 + ATP = oxidized ferredoxin + H2 + NH3 + ADP + phosphate

-

-

1.19.6.1

reduced flavodoxin + N2 + ATP + H2O = oxidized flavodoxin + H2 + NH3 + ADP + phosphate

-

-

1.19.6.1

reduced flavodoxin II + N2 + ATP + H2O = oxidized flavodoxin II + H2 + NH3 + ADP + phosphate

-

-

1.19.6.1

3 entries

1.2.7.4

CO + H2O = CO2 + H2

390485

-

1.8.98.5

reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+ = H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB

-

-

Substrate in Enzyme-catalyzed Reactions (182 results)

top print hide

Show Substrate (182 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.99.30

2-oxo-3,5-dienoates + H2 = ?

655314

-

1.1.99.30

2-oxo-3-(3,4-dihydroxyphenyl)-propionate + H2 = rosmarinic acid

655314

-

1.1.99.30

2-oxo-3-enoates + H2 = ?

655314

-

1.1.99.30

phenylpyruvate + H2 = (2R)-phenyllactate

655314

-

1.12.1.2

FAD + H2 = FADH + H+

439702

-

1.12.1.2

H2 + acceptor = H+ + reduced acceptor

672279, 706499

-

1.12.1.2

H2 + ferricyanide = H+ + ferrocyanide

654782, 439711

-

1.12.1.2

H2 + ferrocyanide = H+ + ferricyanide

655890

-

1.12.1.2

H2 + NAD(P)+ = H+ + NAD(P)H

654821

-

1.12.1.2

H2 + NAD+ = H+ + NADH

439633, 439692, 439694, 439695, 439696, 439697, 439700, 439704, 439705, 439706, 439707, 439709, 439710, 439711, 439712, 656298, 672067, 675493, 726344, 654562, 655890, 674165, 654388, 439703, 439713, 742695, 655307, 742373, 742858, 743797, 685324, 656734, 654821, 654285, 655638, 742575, 439699, 439702, 439708, 439701, 439693, 439698, 654782, 672282, 726282

-

1.12.1.2

H2 + NADP+ = H+ + NADPH

672282

-

1.12.1.2

H2 + oxidized benzyl viologen = H+ + reduced benzyl viologen

439633, 439699, 655890, 656298, 654388, 439713, 439702

-

1.12.1.2

H2 + oxidized benzyl viologen = reduced benzyl viologen + H+

655890, 672282

-

1.12.1.2

H2 + oxidized dithionite = H+ + reduced dithionite

654821

-

1.12.1.2

H2 + oxidized methyl viologen = H+ + reduced methyl viologen

439711, 439699, 654388, 439702, 687403, 655307, 656734, 654285, 655638, 744926, 654821

-

1.12.1.2

H2 + oxidized methyl viologen = reduced methyl viologen + H+

672282

-

1.12.1.2

H2 + oxidized methylene blue = H+ + reduced methylene blue

439711, 654388, 439702

-

1.12.1.2

H2 = H+ + e-

654782

-

1.12.1.2

oxidized methylene blue + H2 = reduced methylene blue + H+

439702

-

1.12.1.3

H2 + 2,6-dichlorophenolindophenol = H+ + ?

659019

-

1.12.1.3

H2 + NAD+ = H+ + NADH

659019

-

1.12.1.3

H2 + NADP+ = H+ + NADPH

704606, 439687, 439688, 659019, 439690, 439691, 439686, 687409

-

1.12.1.3

H2 + oxidized benzyl viologen = H+ + reduced benzyl viologen

687409, 659019

-

1.12.1.3

H2 + oxidized ferredoxin = H+ + reduced ferredoxin

439691

-

1.12.1.3

H2 + oxidized methyl viologen = H+ + reduced methyl viologen

687409, 659019, 439687

-

1.12.1.3

H2 + oxidized methylene blue = H+ + reduced methylene blue

659019

-

1.12.1.4

NAD+ + 2 oxidized ferredoxin + 2 H2 = NADH + 3 H+ + 2 reduced ferredoxin

740625, 740618

-

1.12.1.4

NAD+ + 2 oxidized methyl viologen + 2 H2 = NADH + 3 H+ + 2 reduced methyl viologen

740618

-

1.12.1.4

H2 + NAD+ + oxidized ferredoxin = H+ + NADH + reduced ferredoxin

741931

-

1.12.1.5

H2 + NAD+ = H+ + NADH

439641

-

1.12.1.5

H2 + NADP+ = H+ + NADPH

439641

-

1.12.2.1

ferricytochrome c3 + 2 H2 = ferrocytochrome c3 + 4 H+

701586

-

1.12.2.1

H2 + acceptor = H+ + reduced acceptor

701780

-

1.12.2.1

H2 + ammonium pertechnetate = ?

686422

-

1.12.2.1

H2 + benzyl viologen = H+ + reduced benzyl viologen

395560

-

1.12.2.1

H2 + ferredoxin = H+ + reduced ferredoxin

395559

-

1.12.2.1

H2 + ferricytochrome c = H+ + ferrocytochrome c

672118, 674919, 395568

-

1.12.2.1

H2 + ferricytochrome c = H+ ferrocytochrome c

395568

-

1.12.2.1

H2 + ferricytochrome c3 = H+ + ferrocytochrome c3

395570, 395559, 395567, 395573, 654261, 657318, 395560, 395561, 395562, 395563, 395566, 395571, 395574, 674919, 684509, 686734, 688655, 742006, 395564, 395565, 689932, 395569, 395572, 742696, 395568, 654899, 675360, 672118, 711266, 724456

-

1.12.2.1

H2 + ferricytochrome c3 = H+ ferrocytochrome c3

395568

-

1.12.2.1

H2 + ferricytochrome Hmc = H+ + ferrocytochrome Hmc

654899

-

1.12.2.1

H2 + methyl viologen = H+ + reduced methyl viologen

395565

-

1.12.2.1

H2 + methylene blue = H+ + reduced methylene blue

395568, 395560, 690013

-

1.12.2.1

H2 + methylviologen-dication = H+ + methylviologen

742696

-

1.12.2.1

H2 + mutant cytochrome c K101M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K15M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K26M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K57M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K58M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K60M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K72M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K94M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K95M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c Y65A = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c Y66L = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K101M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K10M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K15M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K26M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K57M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K58M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K60M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K72M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K94M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K95M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 Y65A = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 Y66L = ?

672118

-

1.12.2.1

H2 + oxidized acceptor = H+ + reduced acceptor

704287

-

1.12.2.1

H2 + oxidized dichloroindophenol = H+ + ?

701586

-

1.12.2.1

H2 + oxidized methyl viologen = H+ + reduced methyl viologen

701586

-

1.12.2.1

H2 + potassium ferricyanide = H+ + ?

701586

-

1.12.2.1

H2 + rubredoxin = H+ + reduced rubredoxin

395559

-

1.12.2.1

H2 + [Fe] hydrogenase-cytochrome c3 complex = ?

654899

-

1.12.2.1

H2 + [Fe] hydrogenase-cytochrome Hmc complex = ?

654899

-

1.12.5.1

H2 + 2,3-dimethyl-1,4-naphthoquinone = reduced 2,3-dimethyl-1,4-naphthoquinone

676044, 394192, 394193

-

1.12.5.1

H2 + benzyl viologen = reduced benzyl viologen

394192, 394193, 676044

-

1.12.5.1

H2 + menaquinone = menaquinol

676044

-

1.12.7.2

(SeO3)2- + H2 + ? = Se + H2O + ?

439661

-

1.12.7.2

(TeO3)2- + H2 + ? = Te + H2O + ?

439661

-

1.12.7.2

H2 + 2 oxidized ferredoxin = 2 reduced ferredoxin + 2 H+

730867

-

1.12.7.2

H2 + electron acceptor = H+ + reduced electron acceptor

439668, 439651, 439660, 439643, 439663, 439632, 439669, 439670, 439679, 439681, 439682, 439684, 439671, 439659, 439672, 439665, 439650, 439641, 439652, 439675, 439678, 439674, 439677

-

1.12.7.2

H2 + ferredoxin + oxidized metronidazole = H+ + ferredoxin + reduced metronidazole

673529, 656734

-

1.12.7.2

H2 + methyl viologen = reduced methyl viologen + H+

742859, 742698

-

1.12.7.2

H2 + NAD+ = H+ + NADH

439641

-

1.12.7.2

H2 + oxidized benzyl viologen = H+ + reduced benzyl viologen

439674, 439677

-

1.12.7.2

H2 + oxidized benzyl viologen = reduced benzyl viologen + H+

673529

-

1.12.7.2

H2 + oxidized CAC3527 ferredoxin = H+ + reduced CAC3527 ferredoxin

686267

-

1.12.7.2

H2 + oxidized ferredoxin = H+ + reduced ferredoxin

439643, 685620, 685627, 439665, 439672, 439632, 439669, 439671, 439675, 439679, 439682, 439677, 712281, 712301, 712307, 439654, 688848, 439650, 686267, 684643

-

1.12.7.2

H2 + oxidized ferredoxin = reduced ferredoxin + 2 H+

705334

-

1.12.7.2

H2 + oxidized ferredoxin = reduced ferredoxin + H+

659110, 660222, 742859, 742784, 742698

-

1.12.7.2

H2 + oxidized ferredoxin CAC0587 = H+ + reduced ferredoxin CAC0587

686793

-

1.12.7.2

H2 + oxidized ferredoxin Pet F = reduced ferredoxin Pet F + H+

742279

-

1.12.7.2

H2 + oxidized flavodoxin CAC0587 = H+ + reduced flavodoxin CAC0587

686793

-

1.12.7.2

H2 + oxidized methyl viologen = H+ + reduced methyl viologen

439650, 439669, 439677, 684643, 686267, 686793, 712301, 439654, 688848

-

1.12.7.2

H2 + oxidized methylene blue = H+ + reduced methylene blue

439669

-

1.12.7.2

H2 + oxidized spinach ferredoxin = reduced spinach ferredoxin + H+

742698

-

1.12.98.1

H2 + benzyl viologen = ?

395087, 395100

-

1.12.98.1

H2 + coenzyme F0 = reduced coenzyme F0

395086, 395097, 395089, 395093, 395080

-

1.12.98.1

H2 + coenzyme F420 = reduced coenzyme F420

246745, 659894, 395081, 395084, 395086, 395087, 395089, 395097, 395098, 395095, 395082, 395083, 395085, 395092, 395093, 687420, 395088, 395090, 395100, 395079, 395094, 395080

-

1.12.98.1

H2 + FAD = H+ + FADH2

395093

-

1.12.98.1

H2 + methyl viologen = ?

395080, 395089, 395098, 395095, 395082, 395085, 395093, 395097, 395088

-

1.12.98.1

H2 + oxidized benzyl viologen = reduced benzyl viologen

395099

-

1.12.98.1

H2 + oxidized coenzyme F0 = reduced coenzyme F20

395099

-

1.12.98.1

H2 + oxidized coenzyme F420 = reduced coenzyme F420

742368, 743086, 395099, 727399

-

1.12.98.1

H2 + oxidized methyl viologen = reduced methyl viologen

727399

-

1.1.99.30

4 entries

1.12.1.2

15 entries

1.12.1.3

7 entries

1.12.1.4

3 entries

1.12.1.5

2 entries

1.12.2.1

43 entries

1.12.5.1

3 entries

1.12.7.2

19 entries

1.12.98.1

9 entries

1.12.98.2

5,10-methenyltetrahydromethanopterin + H2 = 5,10-methylenetetrahydromethanopterin + H+

657579, 658635, 658966, 658978, 696971

-

1.12.98.3

H2 + 2-(2,3-dihydropentaprenyloxy)phenazine = 2-dihydropentaprenyloxyphenazine

440300, 440299, 741956, 439643, 727829

-

1.12.98.3

H2 + 2-hydroxyphenazine = dihydro-2-hydroxyphenazine

440299, 440300

-

1.12.98.3

H2 + oxidized methyl viologen = reduced methyl viologen

440297

-

1.12.98.4

H2 + benzyl viologen = H2S + reduced benzyl viologen

439641

-

1.12.98.4

H2 + methyl viologen = H2S + reduced methyl viologen

722289, 439641

-

1.12.98.4

H2 + NADP+ = H2S + NADPH + H+

722289

-

1.12.98.4

H2 + polysulfide(n) = H2S + polysulfide(n-1)

439664, 439641

-

1.12.98.4

polysulfide + H2 = H2S

674514

-

1.12.98.4

S + H2 = H2S

674514

-

1.12.98.4

sulfur + H2 = H2S

659878

-

1.12.98.4

tetrathionate + H2 = ? + H+

674514

-

1.12.98.3

3 entries

1.12.98.4

8 entries

1.12.98.B1

H2 + sulfur = H2S

722508

-

1.12.99.6

H2 + 2,3,5-triphenyltetrazolium chloride = reduced 2,3,5-triphenyltetrazolium chloride

742788

-

1.12.99.6

H2 + 2,3-dimethyl-1,4-naphthoquinone = 2,3-dimethyl-naphthoquinol

659355

-

1.12.99.6

H2 + 2,4,6-trinitrotoluene = ?

675221

-

1.12.99.6

H2 + acceptor = acceptor-H2

684608, 658987, 657739, 659041

-

1.12.99.6

H2 + acceptor = acceptorH2

658082, 659887

-

1.12.99.6

H2 + acceptor = H+ + reduced acceptor

439630, 439644, 439633, 674448, 686733, 711862, 439637, 439628, 684463, 713374, 712029, 439638, 671937, 713368, 439645, 439641, 439627, 439643, 439649, 439647, 439631, 439635, 439640, 439648, 439634, 439632, 439636, 439642, 439646, 439629, 671432, 710927

-

1.12.99.6

H2 + acceptor = H2-acceptor

687302, 684596

-

1.12.99.6

H2 + acceptor = reduced acceptor

742652

-

1.12.99.6

H2 + anthraquinone 2,6-disulfonic acid = anthraquinol 2,6-disulfonic acid

657605

-

1.12.99.6

H2 + benzyl viologen = H+ + reduced benzyl viologen

710951

-

1.12.99.6

H2 + benzyl viologen = reduced benzyl viologen

742788

-

1.12.99.6

H2 + Cr6+ = Cr3+ + H+

657622

-

1.12.99.6

H2 + fumarate = succinate

742788

-

1.12.99.6

H2 + methenyltetrahydromethanopterin = H+ + methylenetetrahydromethanopterin

690060, 687827

-

1.12.99.6

H2 + methionaquinone = methionaquinol + H+

439640

-

1.12.99.6

H2 + methyl viologen = H+ + reduced methyl viologen

725206, 711516

-

1.12.99.6

H2 + methylene blue = reduced methylene blue

742265

-

1.12.99.6

H2 + NAD+ = NADH + H+

439641

-

1.12.99.6

H2 + NADPH = NADP + H+

743732

-

1.12.99.6

H2 + O2 = ?

713401, 710927

-

1.12.99.6

H2 + oxidized 2,3-dimethylnaphthoquinone = 2,3-dimethylnaphthoquinol

659878

-

1.12.99.6

H2 + oxidized acceptor = H+ + reduced acceptor

704016, 701778, 704489, 702254, 704162

-

1.12.99.6

H2 + oxidized amaranth = H+ + reduced amaranth

684651

-

1.12.99.6

H2 + oxidized benzyl viologen = H+ + reduced benzyl viologen

439648, 686732, 702218, 702707, 703746

-

1.12.99.6

H2 + oxidized benzyl viologen = H+ + reduceded benzyl viologen

685293

-

1.12.99.6

H2 + oxidized benzyl viologen = reduced benzyl viologen

658082

-

1.12.99.6

H2 + oxidized benzyl viologen = reduced benzyl viologen + H+

658082, 659355, 657605, 659878, 658138, 672638

-

1.12.99.6

H2 + oxidized dichloroindophenol = H+ + reduced dichloroindophenol

711516

-

1.12.99.6

H2 + oxidized diquat = reduced diquat + H+

657605

-

1.12.99.6

H2 + oxidized electron acceptor = H+ + reduced electron acceptor

702307, 704015, 704013, 704012, 687302

-

1.12.99.6

H2 + oxidized ethyl viologen = reduced ethyl viologen + H+

657605

-

1.12.99.6

H2 + oxidized ferredoxin = reduced ferredoxin

439643

-

1.12.99.6

H2 + oxidized methionaquinone = reduced methionaquinone

439640

-

1.12.99.6

H2 + oxidized methyl viologen = H+ + reduced methyl viologen

706442, 684608, 726704, 704309, 688848, 704744, 703746

-

1.12.99.6

H2 + oxidized methyl viologen = reduced methyl viologen

439647

-

1.12.99.6

H2 + oxidized methyl viologen = reduced methyl viologen + H+

657778, 672729, 658972, 657622, 657605, 659878, 659310, 659520, 658138

-

1.12.99.6

H2 + oxidized methylene blue = H+ + reduced methylene blue

711516, 704518, 703746

-

1.12.99.6

H2 + oxidized methylene blue = reduced methylene blue

439646, 703746

-

1.12.99.6

H2 + oxidized methylene blue = reduced methylene blue + H+

674936, 703746

-

1.12.99.6

H2 + oxidized metranidazole = H+ + reduced metranidazol

659523

-

1.12.99.6

H2 + oxidized phenazine methosulfate = reduced phenazine methosulfate + H+

657605

-

1.12.99.6

H2 + oxidized sodium hydrosulfite = H+ + reduced sodium hydrosulfite

711516

-

1.12.99.6

H2 + phenazine methosulfate = H+ + reduced phenazine methosulfate

711516

-

1.12.99.6

H2 + S = H2S

439641

-

1.12.99.6

44 entries

1.14.14.9

2-phenylbutyric acid + H2 = ?

438895

-

1.18.6.2

acetylene + H2 + ? = ethylene + ?

745388

-

1.2.1.B29

H2 + NADP+ + 2 oxidized ferredoxin [iron-sulfur] cluster = NADPH + 3 H+ + 2 reduced ferredoxin [iron-sulfur] cluster

734087

-

1.2.1.B29

H2 + NADP+ + 2 oxidized methyl viologen = ? + NADPH + H+ + 2 reduced methyl viologen

734087

-

1.2.1.B29

2 entries

1.2.7.4

H2 + reduced methyl viologen = methyl viologen

655446

-

1.21.99.5

tetrachloroethene + 2 H2 = cis-1,2-dichloroethene + 2 chloride + 2 H+

657605

-

1.8.98.1

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 = coenzyme B + coenzyme M

655396, 655400, 655398, 655471, 655531, 655825, 654385, 639391

-

1.8.98.5

coenzyme B-coenzyme M disulfide + ferredoxin + 2 H2 = coenzyme B + coenzyme M + reduced ferredoxin2- + 2 H+

726376

-

1.8.98.5

coenzyme B-coenzyme M disulfide + metronidazole + 2 H2 = coenzyme B + coenzyme M + reduced metronidazole2- + 2 H+

726376

-

1.8.98.5

H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB = reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+

744833, 744179

-

1.8.98.5

H2 + oxidized methyl viologen + CoM-S-S-CoB = reduced methyl viologen + CoB + CoM + H+

744833, 744179

-

1.8.98.5

4 entries

1.97.1.9

selenate + H2 = selenite + H2O

658902

-

3.1.2.1

H2 + CO2 + CH3-H4SPt + CoASH = acetyl-CoA + H4SPt + H2O

94491

-

3.1.3.7

3'-phosphoadenosine 5'-phosphate + H2 = AMP + phosphate

730884

-

3.4.21.B12

urokinase-type plasminogen activator receptor + H2 = ?

664270

-

3.4.22.15

fibronectin + H2 = ?

727746

-

3.4.22.15

laminin + H2 = ?

727746

-

3.4.22.15

vitronectin + H2 = ?

727746

-

3.4.22.15

3 entries

3.4.22.B6

fibronectin + H2 = ?

754980

-

7.1.1.9

reduced N,N,N',N'-tetramethyl-4-phenylenediamine + O2 + H2 = oxidized N,N,N',N'-tetramethyl-4-phenylenediamine + H2O

743382

-

Product in Enzyme-catalyzed Reactions (56 results)

top print hide

Show Product (56 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.37

(S)-malate + NAD+ = acetate + CO2 + H2 + NADH

286670

-

1.11.1.16

2 Mn2+ + 2 H+ = 2 Mn3+ + H2

-

-

1.12.1.2

H+ + NADH + reduced ferredoxin = H2 + NAD+ + oxidized ferredoxin

-

-

1.12.1.2

H+ + NADH = H2 + NAD+

-

-

1.12.1.2

H+ + NADPH = H2 + NADP+

-

-

1.12.1.2

H+ + reduced dithionite = H2 + oxidized dithionite

-

-

1.12.1.2

H+ + reduced methyl viologen = H2 + methyl viologen

-

-

1.12.1.2

H+ + reduced methyl viologen = H2 + oxidized methyl viologen

-

-

1.12.1.3

H+ + NADPH = H2 + NADP+

-

-

1.12.1.3

H+ + reduced benzyl viologen = H2 + oxidized benzyl viologen

-

-

1.12.1.3

NADPH + H+ = H2 + NADP+

-

-

1.12.1.4

NADH + 3 H+ + 2 reduced ferredoxin = NAD+ + 2 oxidized ferredoxin + 2 H2

-

-

1.12.1.4

NADH + 3 H+ + 2 reduced methyl viologen = NAD+ + 2 oxidized methyl viologen + 2 H2

-

-

1.12.1.4

NADH + H+ + reduced ferredoxin = H2 + NAD+ + oxidized ferredoxin

-

-

1.12.1.4

NADH + H+ + reduced methyl viologen = H2 + NAD+ + oxidized methyl viologen

-

-

1.12.1.4

sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid = H2 + oxidized anthraquinone-2,6-disulfonic acid + ?

-

-

1.12.1.4

sodium dithionite + H+ + reduced methyl viologen = H2 + oxidized methyl viologen + ?

-

-

1.12.1.5

H+ + NADPH = H2 + NADP+

-

-

1.12.1.5

H+ + reduced methyl viologen = H2 + oxidized methyl viologen

-

-

1.12.2.1

H+ + ferrocytochrome c3 = H2 + ferricytochrome c3

-

-

1.12.2.1

H+ + neutral red = H2 + oxidized neutral red

-

-

1.12.2.1

H+ + phenosafranine = H2 + oxidized phenosafranine

-

-

1.12.2.1

H+ + reduced methyl viologen = H2 + methyl viologen

-

-

1.12.2.1

reduced methyl viologen + H+ = oxidized methyl viologen + H2

-

-

1.12.7.2

2 H+ + reduced ferredoxin = H2 + oxidized ferredoxin

-

-

1.12.7.2

2 H+ + reduced polyferredoxin = H2 + oxidized polyferredoxin

-

-

1.12.7.2

H+ + NADH = H2 + NAD+

-

-

1.12.7.2

H+ + reduced benzyl viologen = H2 + oxidized benzyl viologen

-

-

1.12.7.2

H+ + reduced ferredoxin = H2 + oxidized ferredoxin

-

-

1.12.7.2

H+ + reduced methyl viologen = H2 + oxidized methyl viologen

-

-

1.12.98.2

H+ + 5,10-methylenetetrahydromethanopterin = H2 + 5,10-methenyltetrahydromethanopterin

-

-

1.12.98.2

N5,N10-methylenetetrahydromethanopterin + H+ = N5,N10-methenyltetrahydromethanopterin + H2

, 394696

-

1.12.98.4

H2S + NADH + H+ = H2 + NAD+

-

-

1.12.98.4

H2S + NADPH + H+ = H2 + NADP+

-

-

1.12.99.6

formate = H2 + CO2

-

-

1.12.99.6

reduced ferredoxin = H2 + oxidized ferredoxin

-

-

1.12.1.2

6 entries

1.12.1.3

3 entries

1.12.1.4

6 entries

1.12.1.5

2 entries

1.12.2.1

5 entries

1.12.7.2

6 entries

1.12.98.2

2 entries

1.12.98.4

2 entries

1.12.99.6

2 entries

1.14.14.32

17alpha-hydroxypregnenolone + [reduced NADPH-hemoprotein reductase] + O2 = 3beta-hydroxyandrost-5-en-17-one + acetate + [oxidized NADPH-hemoprotein reductase] + H2

-

-

1.18.6.1

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

, 440145, 440134, 440143, 440144, 440151, 440142, 440171, 440176, 440177, 440140, 440146, 440147, 440149, 440152, 440157, 440166, 440174, 440153, 440159, 440164, 440180, 440150, 440155, 440156, 440158, 440163, 440178, 440179, 440181, 440183, 440184, 440187, 440188, 440189, 440169, 440162, 440148, 440165, 440170, 440161, 440167, 440168, 440160, 440138, 440154, 440186, 440175, 440182

-

1.18.6.1

C2H2 + ? = H2 + ?

-

-

1.18.6.1

N2 + 10 H+ + 8 e- + 16 ATP = 2 NH4+ + H2 + 16 ADP + 16 phosphate

-

-

1.18.6.1

N2 + 8 e- + 16 ATP + 8 H+ = 2 NH3 + H2 + 16 ADP + 16 phosphate

-

-

1.18.6.1

N2 + 8 e- + 8 H+ + 16 ATP = 2 NH3 + H2 + 16 ADP + 16 phosphate

-

-

1.18.6.1

reduced ferredoxin + H+ + ATP = oxidized ferredoxin + H2 + ADP + phosphate

440184, 440187, 440189, , 440176

-

1.18.6.1

reduced ferredoxin + H+ + N2 + ATP + H2O = oxidized ferredoxin + H2 + NH3 + ADP + phosphate

-

-

1.18.6.1

reduced ferredoxin + H+ + N2 + ATP = oxidized ferredoxin + H2 + NH3 + ADP + phosphate

-

-

1.18.6.2

12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O = 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate

-

-

1.18.6.2

CO + H+ + ATP = C2H4 + C2H6 + C3H8 + H2O + H2 + phosphate

-

-

1.19.6.1

reduced ferredoxin + H+ + N2 + ATP = oxidized ferredoxin + H2 + NH3 + ADP + phosphate

-

-

1.19.6.1

reduced flavodoxin + N2 + ATP + H2O = oxidized flavodoxin + H2 + NH3 + ADP + phosphate

-

-

1.19.6.1

reduced flavodoxin II + N2 + ATP + H2O = oxidized flavodoxin II + H2 + NH3 + ADP + phosphate

-

-

1.18.6.1

8 entries

1.18.6.2

2 entries

1.19.6.1

3 entries

1.2.1.B29

NADPH + 3 H+ + 2 reduced ferredoxin [iron-sulfur] cluster = H2 + NADP+ + 2 oxidized ferredoxin [iron-sulfur] cluster

-

-

1.2.7.4

CO + H2O + methylene blue = CO2 + reduced methylene blue + H2

-

-

1.2.7.4

CO + H2O = CO2 + H2

390485

-

1.2.7.4

2 entries

1.8.98.1

coenzyme B + coenzyme M + reduced ferredoxin + H+ + coenzyme F420 = coenzyme B-coenzyme M disulfide + ferredoxin + H2 + reduced coenzyme F420

-

-

1.8.98.5

reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+ = H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB

-

-

1.8.98.5

reduced metronidazole + CoB + CoM + H+ = H2 + oxidized metronidazole + CoM-S-S-CoB

-

-

1.8.98.5

2 entries

Activator in Enzyme-catalyzed Reactions (2 results)

top print hide

Show Activating Compound (2 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

2.8.4.1

suggested to be involved in the activation of the methylreductase, preincubation

393257

-

7.2.2.11

stimulates

210289

-

Inhibitor in Enzyme-catalyzed Reactions (9 results)

top print hide

Show Inhibitors (9 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

1.12.98.2

inhibits formation of N5,N10-methenyltetrahydromethanopterin

394694

-

1.12.99.6

inhibits hydrogen production

674190

-

1.18.6.1

-

440144, 440146, 440154, 440158

-

1.18.6.1

competitive

440183

-

1.18.6.1

competitive inhibition of N2 binding

658040

-

1.18.6.1

competitive inhibitor of N2, no inhibition of N3-, C2H2, CN- or H+ reduction

440146

-

1.18.6.1

H2 competes with N2 binding and inhibits N2 reduction by the FeMo protein, but H2 does not inhibit NO2- reduction for the wild-type or either of the two MoFe protein variants

742740

-

1.18.6.1

5 entries

1.18.6.2

CO-reduction by V-nitrogenase is inhibited by the addition of increasing amounts of H2

746492

-

1.19.6.1

inhibits both N2 and diazene reduction

685165

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (11 results)

top print hide

Show Turnover Number (11 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.12.1.2

70

-

pH 8.0, HoxI deletion mutant enzyme, with oxidized benzyl viologen

655890

1.12.1.2

109

-

pH 8.0, HoxI deletion mutant enzyme, with NAD+

655890

1.12.1.2

120

-

pH 8.0, wild-type enzyme complex including HoxI, with oxidized benzyl viologen

655890

1.12.1.2

143

-

pH 8.0, wild-type enzyme complex including HoxI, with NAD+

655890

1.12.1.2

212

-

-

439706

1.12.1.2

284

-

pH 8.0, HoxI deletion mutant enzyme, with ferrocyanide

655890

1.12.1.2

372

-

pH 8.0, wild-type enzyme complex including HoxI, with ferrocyanide

655890

1.12.7.2

0.683

-

-

439659

1.12.7.2

133

-

using ferredoxin CAC0587 as electron carrier, at 35°C

686793

1.12.7.2

454

-

using methyl viologen as electron carrier, at 35°C

686793

1.12.7.2

483

-

using flavodoxin CAC0587 as electron carrier, at 35°C

686793

1.12.1.2

7 entries

1.12.7.2

4 entries

K_M Value (39 results)

top print hide

Show KM Value (39 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.12.1.2

0.037

-

-

439699

1.12.1.2

0.063

-

-

439701

1.12.1.2

0.19

-

-

439693

1.12.1.3

0.012

-

-

439691

1.12.1.3

0.018

-

cosubstrate: benzyl viologen

687409

1.12.1.3

0.02

-

cosubstrate: methyl viologen

687409

1.12.1.2

3 entries

1.12.1.3

3 entries

1.12.1.4

-999

-

apparent Km value for H2 is about 6% in the gas phase, pH 7.5, 45°C

740618

1.12.7.2

0.000033

-

using ferredoxin CAC0587 as electron carrier, at 35°C

686793

1.12.7.2

0.000088

-

using flavodoxin CAC0587 as electron carrier, at 35°C

686793

1.12.7.2

0.005

-

-

439643, 659110

1.12.7.2

0.016

-

using methyl viologen as electron carrier, at 35°C

686793

1.12.7.2

0.341

-

-

439659

1.12.7.2

5.6

-

-

439663

1.12.98.1

0.002

-

+ methyl viologen, pH 9

395080

1.12.98.1

0.003

-

37°C, pH 7.2, cosubstrate: oxidized coenzyme F420

395099

1.12.98.1

0.004

-

37°C, pH 7.2, cosubstrate: oxidized methyl viologen

395099

1.12.98.1

0.01

-

+ coenzyme F0, pH 7.2

395080

1.12.98.1

0.012

-

-

395089

1.12.98.2

-999

-

apparent Km for H2 is 60%

727402

1.12.98.2

-999

-

the apparent Km for H2 is estimated to be approximately 60% H2 in the gas phase

727402

1.12.98.2

0.15

-

-

394696

1.12.99.6

0.000047

-

apparent value, pH and temperature not specified in the publication

711862

1.12.99.6

0.0004

-

at pH 5.5, 10°C

713401

1.12.99.6

0.001

-

-

439646

1.12.99.6

0.001

-

at pH 5.5, 20°C

713401

1.12.99.6

0.002

-

-

439648

1.12.99.6

0.005

-

-

439643

1.12.99.6

0.006

-

at pH 4.5, 30°C

713401

1.12.99.6

0.006

-

at pH 5.5, 30°C

713401

1.12.99.6

0.008

-

at pH 5.0, 30°C

713401

1.12.99.6

0.01

-

at pH 6.5, 30°C

713401

1.12.99.6

0.0113

-

-

439647

1.12.99.6

0.015

-

at pH 6.0, 30°C

713401

1.12.99.6

0.024

-

at pH 5.5, 40°C

713401

1.12.99.6

0.08

-

with methylene blue as electron acceptor

439632

1.12.99.6

0.41

-

with methyl viologen as electron acceptor

439634

1.12.99.6

1.9

-

with methyl viologen as electron acceptor

439632

1.12.99.6

25

-

-

439631

1.12.7.2

6 entries

1.12.98.1

5 entries

1.12.98.2

3 entries

1.12.99.6

17 entries

1.8.98.1

0.03

-

pH 7, 65°C, H2: heterodisulfide oxidoreductase complex

655398

References & Links

Literature References (344)

top print hide

Show Reference (344 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

94491

7718564

Substrate and accessory protein requirements and thermodynamics of acetyl-CoA synthesis and cleavage in Methanosarcina barkeri

1995

Grahame, D.A.; de Moll, E.

Biochemistry

34

4617-4624

210289

2757374

Nickel transport by the thermophilic acetogen Acetogenium kivui

1989

Yang, H.; Daniel, S.L.; Hsu, T.; Drake, H.L.

Appl. Environ. Microbiol.

55

1078-1081

246745

-

Effect of methanogenic substrates on coenzyme f420-dependent N5,N10-methylene-H4MPT dehydrogenase, N5,N10-methenyl-H4MPT cyclohydrolase and F420-reducing hydrogenase activities in Methanosarcina barkeri

1993

Mukhopadhyay, B.; Purwantini, E.; Daniels, L.

Arch. Microbiol.

159

141-146

286670

9269720

Isolation and characterization of cytosolic malate dehydrogenase from Trichomonas vaginalis

1997

Drmota, T.; Tachezy, J.; Kulda, J.

Folia Parasitol.

44

103-108

390485

11509720

Crystal structure of a carbon monoxide dehydrogenase reveals a [Ni-4Fe-5S] cluster

2001

Dobbek, H.; Svetlitchnyi, V.; Gremer, L.; Huber, R.; Meyer, O.

Science

293

1281-1285

393257

3137210

Reductive activation of the methyl coenzyme M methylreductase system of Methanobacterium thermoautotrophicum.DELTA.H

1988

Rouviere, P.E.; Bobik, T.A.; Wolfe, R.S.

J. Bacteriol.

170

3946-3952

394192

1587288

The quinone-reactive Ni/Fe-hydrogenase of Wolinella succinogenes

1992

Dross, F.; Geisler, V.; Lenger, R.; Theis, F.; Krafft, T.; Fahrenholz, F.; Kojro, E.; Duchene, A.; Tripier, D.; Juvenal, K.; Krueger, A.

Eur. J. Biochem.

206

93-102

394193

8319698

Erratum

1993

Dross, F.; Geisler, V.; Lenger, R.; Theis, F.; Krafft, T.; Fahrenholz, F.; Kojro, E.; Duchene, A.; Tripier, D.; Juvenal, K.; Krueger, A.

Eur. J. Biochem.

214

949-950

394694

-

N5,N10-Methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum has hydrogenase activity

1990

Zirngibl, C.; Hedderich, R.; Thauer, R.K.

FEBS Lett.

261

112-116

394696

8599536

Purification, properties and primary structure of H2-forming N5,N10-methylenetetrahydromethanopterin dehydrogenase from Methanococcus thermolithotrophicus

1996

Hartmann, G.C.; Klein, A.R.; Linder, M.; Thauer, R.K.

Arch. Microbiol.

165

187-193

395079

6211447

A selenium-containing hydrogenase from Methanococcus vannielii. Identification of the selenium moiety as a selenocysteine residue

1982

Yamazaki, S.

J. Biol. Chem.

257

7926-7929

395080

7061485

Purification and properties of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum

1982

Jacobson, F.S.; Daniels, L.; Fox, J.A.; Walsh, C.T.; Orme-Johnson, W.H.

J. Biol. Chem.

257

3385-3388

395081

6300837

Paramagnetic centers in the nickel-containig, deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum

1983

Kojima, N.; Fox, J.A.; Hausinger, R.P.; Daniels, L.; Orme-Johnson, W.H.; Walsh, C.

Proc. Natl. Acad. Sci. USA

80

378-382

395082

-

Two hydrogenases with distinct electron-carrier specificity and subunit composition in Methanobacterium formicicum

1983

Jin, S.L.C.; Blanchard, D.K.; Chen, J.S.

Biochim. Biophys. Acta

748

8-20

395083

6375661

FAD requirement for the reduction of coenzyme F420 by hydrogenase from Methanobacterium formicicum

1984

Nelson, M.J.K.; Brown, D.P.; Ferry, J.G.

Biochem. Biophys. Res. Commun.

120

775-781

395084

3804976

Electron microscopy of nickel-containing methanogenic enzymes: methyl reductase and F420-reducing hydrogenase

1987

Wackett, L.P.; Hartwieg, E.A.; King, J.A.; Orme-Johnson, W.H.; Walsh, C.T.

J. Bacteriol.

169

718-727

395085

3301819

Locations of the hydrogenases of Methanobacterium formicicum after subcellular fractionation of cell extract

1987

Baron, S.F.; Brown, D.P.; Ferry, J.G.

J. Bacteriol.

169

3823-3825

395086

3663585

8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization

1987

Fox, J.A.; Livingston, D.J.; Orme-Johnson, W.H.; Walsh, C.T.

Biochemistry

26

4219-4227

395087

-

Properties of the particulate enzyme F420-reducing hydrogenase isolated from Methanospirillum hungatei

1987

Sprott, G.D.; Shaw, K.M.; Beveridge, T.J.

Can. J. Microbiol.

33

896-904

395088

3121317

Purification and characterization of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from the archaebacterium Methanococcus voltae

1987

Muth, E.; Mörschel, E.; Klein, A.

Eur. J. Biochem.

169

571-577

395089

3663586

8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 2. Kinetic and hydrogen-transfer studies

1987

Livingston, D.J.; Fox, J.A.; Orme-Johnson, W.H.; Walsh, C.T.

Biochemistry

26

4228-4237

395090

-

Localization of the F420-reducing hydrogenase in Methanococcus voltae cells by immuno-gold technique

1988

Muth, E.

Arch. Microbiol.

150

205-207

395092

-

Immunogold localization of coenzyme F420-reducing format dehydrogenase and coenzyme F420-reducing hydrogenase in Methanobacterium formicicum

1989

Baron, S.F.; Williams, D.S.; May, H.D.; Patel, P.S.; Aldrich, H.C.; Ferry, J.G.

Arch. Microbiol.

151

307-313

395093

2738024

Purification and properties of the membrane-associated coenzyme F420-reducing hydrogenase from Methanobacterium formicicum

1989

Baron, S.F.; Ferry, J.G.

J. Bacteriol.

171

3846-3853

395094

2207102

Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta H

1990

Alex, L.A.; Reeve, J.N.; Orme-Johnson, W.H.; Walsh, C.T.

Biochemistry

29

7237-7244

395095

16348172

Partial purification and characterization of two hydrogenases from the extreme thermophile Methanococcus jannaschii

1990

Shah, N.N.; Clark, D.S.

Appl. Environ. Microbiol.

56

858-863

395097

27316897

Some properties of F420-reducing hydrogenase of Methanobacterium thermoformicicum strain SF-4

1993

Tachibana, A.; Tanaka, T.; Taniguchi, M.; Oi, S.

Biosci. Biotechnol. Biochem.

57

156-157

395098

8002593

Structural aspects and immunolocalization of the F420-reducing and non-F420-reducing hydrogenases from Methanobacterium thermoautotrophicum Marburg

1994

Braks, I.J.; Hoppert, M.; Roge, S.; Mayer, F.

J. Bacteriol.

176

7677-7687

395099

8521835

Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro

1995

Michel, R.; Massanz, C.; Kostka, S.; Richter, M.; Fiebig, K.

Eur. J. Biochem.

233

727-735

395100

11054113

Conversion of the central [4Fe-4S] cluster into a [3Fe-4S] cluster leads to reduced hydrogen-uptake activity of the F420-reducing hydrogenase of Methanococcus voltae

2000

Bingemann, R.; Klein, A.

Eur. J. Biochem.

267

6612-6618

395559

6093686

Hydrogenase, electron-transfer proteins, and energy coupling in the sulfate-reducing bacteria Desulfovibrio

1984

Odom, J.M.; Peck, H.D.

Annu. Rev. Microbiol.

38

551-592

395560

6303306

Purification and properties of the membrane-bound by hydrogenase from Desulfovibrio desulfuricans

1983

Lalla-Maharajh, W.V.; Hall, D.O.; Cammack, R.; Rao, K.K.

Biochem. J.

209

445-454

395561

5650411

Purification and properties of hydrogenases of different origins

1968

Yagi, T.; Honya, M.; Tamiya, N.

Biochim. Biophys. Acta

153

699-705

395562

181372

Properties of purified hydrogenase from the particulate fraction of Desulfovibrio vulgaris, Miyazaki

1976

Yagi, T.; Kimura, K.; Daidoji, H.; Sakai, F.; Tamura, S.; Inokuchi, H.

J. Biochem.

79

661-671

395563

6339237

Kinetic properties of hydrogenase isolated from Desulfovibrio vulgaris (Hildenborough)

1983

Grande, H.J.; van Berkel-Arts, A.; Bregh, J.; van Dijk, K.; Veeger, C.

Eur. J. Biochem.

131

81-88

395564

-

Kinetic studies of hydrogenase in AOT reversed micelles

1989

Castro, M.J.M.; Cabral, J.M.S.

Enzyme Microb. Technol.

11

6-11

395565

-

Stability of hydrogenase in AOT reversed micelles

1989

Castro, M.J.M.; Cabral, J.M.S.

Enzyme Microb. Technol.

11

668-672

395566

3546297

Single crystals of hydrogenase from Desulfovibrio vulgaris Miyazaki F

1987

Higuchi, Y.; Yasuoka, N.; Kakudo, M.; Katsube, Y.; Yagi, T.; Inokuchi, H.

J. Biol. Chem.

262

2823-2825

395567

6096145

Purification and properties of the soluble hydrogenase from Desulfovibrio desulfuricans (strain Norway 4)

1984

Rieder, R.; Cammack, R.; Hall, D.O.

Eur. J. Biochem.

145

637-643

395568

11170458

Nonaheme cytochrome c, a new physiological electron acceptor for [Ni,Fe] hydrogenase in the sulfate-reducing bacterium Desulfovibrio desulfuricans Essex: primary sequence, molecular parameters, and redox properties

2001

Fritz, G.; Griesshaber, D.; Seth, O.; Kroneck, P.M.

Biochemistry

40

1317-1324

395569

-

Hydrogenase of purple bacteria: properties and regulation of synthesis

1984

Gogotov, I.N.

Arch. Microbiol.

140

86-90

395570

369919

Solubilization and partial purification of the membrane-bound hydrogenase of Escherichia coli

1978

Adams, M.W.W.; Hall, D.O.

Biochem. Soc. Trans.

6

1339-1341

395571

10606770

A sequential electron transfer from hydrogenases to cytochromes in sulfate-reducing bacteria

2000

Aubert, C.; Brugna, M.; Dolla, A.; Bruschi, M.; Giudici-Orticoni, M.T.

Biochim. Biophys. Acta

1476

85-92

395572

11571159

Reduction of technetium(VII) by Desulfovibrio fructosovorans is mediated by the nickel-iron hydrogenase

2001

De Luca, G.; De Philip, P.; Dermoun, Z.; Rousset, M.; Vermegljo, A.

Appl. Environ. Microbiol.

67

4583-4587

395573

-

Kinetic properties of the periplasmic hydrogenase from Desulfovibrio desulfuricans NCIMB 8372 and use in photosensitized hydrogen-production

1993

Eng, L.H.; Lewin, M.B.M.; Neujahr, H.Y.

J. Chem. Technol. Biotechnol.

56

317-324

395574

10049702

Liberation of hydrogen sulfide during the catalytic action of Desulfovibrio hydrogenase under the atmosphere of hydrogen

1999

Higuchi, Y.; Yagi, T.

Biochem. Biophys. Res. Commun.

255

295-299

438895

-

Purification and characterization of the 4-hydroxyphenylacetic acid-3-hydroxylase from Pseudomonas putida U

1997

Fernandez-Medarde, A.; Luengo, J.M.

FEMS Microbiol. Lett.

157

47-53

439627

2822711

The mechanisms of hydrogen activation and carbon monoxide binding by hydrogenase I and hydrogenase II of Clostridium pasteurianum

1987

Adams, M.W.W.

J. Biol. Chem.

262

15054-15061

439628

3015247

On the novel hydrogen-activating iron-sulfur center of the "iron-only" hydrogenases

1986

Adams, M.W.W.; Johnson, M.K.; Zambrano, I.C.; Mortenson, L.E.

Biochimie

68

35-41

439629

27281001

Characterization and thermostability of a membrane-bound hydrogenase from a thermophilic hydrogen oxidizing bacterium, Bacillus schlegelii

1993

Aono, S.; Kamachi, T.; Okura, I.

Biosci. Biotechnol. Biochem.

57

1177-1179

439630

3894325

Nickel-containing hydrogenase isoenzymes from anaerobically grown Escherichia coli K-12

1985

Ballantine, S.P.; Boxer, D.H.

J. Bacteriol.

163

454-459

439631

11278570

The H2 sensor of Ralstonia eutropha. Biochemical characteristics, spectroscopic properties, and its interaction with a histidine protein kinase

2001

Bernhard, M.; Buhrke, T.; Bleijlevens, B.; De Lacey, A.L.; Fernandez, V.M.; Albracht, S.P.J.; Friedrich, B.

J. Biol. Chem.

276

15592-15597

439632

2538471

Characterization of hydrogenase from the hyperthermophilic archaebacterium, Pyrococcus furiosus

1989

Bryant, F.O.; Adams, M.W.W.

J. Biol. Chem.

264

5070-5079

439633

12399498

Functional analysis by site-directed mutagenesis of the NAD+-reducing hydrogenase from Ralstonia eutropha

2002

Burgdorf, T.; De Lacey, A.L.; Friedrich, B.

J. Bacteriol.

184

6280-6288

439634

6097250

A cytoplasmic nickel-iron hydrogenase with high specific activity from Desulfovibrio multispirans sp. n., a new species of sulfate reducing bacterium

1984

Czechowski, M.H.; He, S.H.; Nacro, M.; DerVartanian, D.V.; Peck, H.D., Jr.; LeGall, J.

Biochem. Biophys. Res. Commun.

125

1025-1032

439635

1317168

Partial purification and characterization of the first hydrogenase isolated from a thermophilic sulfate-reducing bacterium

1992

Fauque, G.; Czechowski, M.; Berlier, Y.M.; Lespinat, P.A.; LeGall, J.; Moura, J.J.G.

Biochem. Biophys. Res. Commun.

184

1256-1260

439636

3015248

Activation and deactivation of the membrane-bound hydrogenase from Desulfovibrio desulfuricans, Norway strain

1986

Fernandez, V.M.; Rao, K.K.; Fernandez, M.A.; Cammack, R.

Biochimie

68

43-48

439637

12515509

Single crystal EPR studies of the reduced active site of [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F

2003

Foerster, S.; Stein, M.; Brecht, M.; Ogata, H.; Higuchi, Y.; Lubitz, W.

J. Am. Chem. Soc.

125

83-93

439638

9479448

Hydrogenase: a hydrogen-metabolizing enzyme. What do the crystal structures tell us about its mode of action?

1997

Fontecilla-Camps, J.C.; Frey, M.; Garcin, E.; Hatchikian, C.; Montet, Y.; Piras, C.; Vernede, X.; Volbeda, A.

Biochimie

79

661-666

439640

10803945

Purification and characterization of membrane-bound hydrogenase from Hydrogenobacter thermophilus strain TK-6, an obligately autotrophic, thermophilic, hydrogen-oxidizing bacterium

2000

Ishii, M.; Takishita, S.; Iwasaki, T.; Peerapornpisal, Y.; Yoshino, J.I.; Kodama, T.; Igarashi, Y.

Biosci. Biotechnol. Biochem.

64

492-502

439641

10714990

Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction

2000

Ma, K.; Weiss, R.; Adams, M.W.W.

J. Bacteriol.

182

1864-1871

439642

1327776

Further characterization of the [iron]-hydrogenase from Desulfovibrio desulfuricans ATCC 7757

1992

Hatchikian, E.C.; Forget, N.; Fernandez, V.M.; Williams, R.; Cammack, R.

Eur. J. Biochem.

209

357-365

439643

10491189

Purification and catalytic properties of Ech hydrogenase from Methanosarcina barkeri

1999

Meuer, J.; Bartoschek, S.; Koch, J.; Kunkel, A.; Hedderich, R.

Eur. J. Biochem.

265

325-335

439644

10694885

A novel FeS cluster in Fe-only hydrogenases

2000

Nicolet, Y.; Lemon, B.J.; Fontecilla-Camps, J.C.; Peters, J.W.

Trends Biochem. Sci.

25

138-143

439645

12296727

Structural studies of the carbon monoxide complex of [NiFe]hydrogenase from Desulfovibrio vulgaris Miyazaki F: Suggestion for the initial activation site for dihydrogen

2002

Ogata, H.; Mizoguchi, Y.; Mizuno, N.; Miki, K.; Adachi, S.I.; Yasuoka, N.; Yagi, T.; Yamauchi, O.; Hirota, S.; Higuchi, Y.

J. Am. Chem. Soc.

124

11628-11635

439646

-

Oxygen effects on the nickel- and iron-containing hydrogenase from Azotobacter vinelandii

1989

Seefeldt, L.C.; Arp, D.J.

Biochemistry

28

1588-1596

439647

8612797

Reversible hydrogenase of Anabaena variabilis ATCC 29413. Catalytic properties and characterization of redox centers

1996

Serebryakova, L.T.; Medina, M.; Zorin, N.A.; Gogotov, I.N.; Cammack, R.

FEBS Lett.

383

79-82

439648

3516689

Purification and properties of membrane-bound hydrogenase isoenzyme 1 from anaerobically grown Escherichia coli K12

1986

Sawers, R.G.; Boxer, D.H.

Eur. J. Biochem.

156

265-275

439649

9324252

Purification and molecular characterization of the H2 uptake membrane-bound NiFe-hydrogenase from the carboxidotrophic bacterium Oligotropha carboxidovorans

1997

Santiago, B.; Meyer, O.

J. Bacteriol.

179

6053-6060

439650

11096090

A novel type of iron hydrogenase in the green alga Scenedesmus obliquus is linked to the photosynthetic electron transport chain

2001

Florin, L.; Tsokoglou, A.; Happe, T.

J. Biol. Chem.

276

6125-6132

439651

11456963

Mossbauer characterization of the iron-sulfur clusters in Desulfovibrio vulgaris hydrogenase

2001

Pereira, A.S.; Tavares, P.; Moura, I.; Moura, J.J.; Huynh, B.H.

J. Am. Chem. Soc.

123

2771-2782

439652

10852873

Purification and characterization of a membrane-bound hydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus

2000

Sapra, R.; Verhagen, M.F.; Adams, M.W.

J. Bacteriol.

182

3423-3428

439654

9989593

Purification and characterization of hydrogenase from the marine green alga, Chlorococcum littorale

1999

Ueno, Y.; Kurano, N.; Miyachi, S.

FEBS Lett.

443

144-148

439659

8593101

Purification and characterization of a membrane-bound hydrogenase from Sporomusa sphaeroides involved in energy-transducing electron transport

1996

Dobrindt, U.; Blaut, M.

Arch. Microbiol.

165

141-147

439660

8661919

Purification and characterization of the hydrogenase from Thiobacillus ferrooxidans

1996

Fischer, J.; Quentmeier, A.; Kostka, S.; Kraft, R.; Friedrich, C.G.

Arch. Microbiol.

165

289-296

439661

16887509

Hydrogenase I of Clostridium pasteurianum functions as a novel selenite reductase

1995

Yanke, L.J.; Bryant, R.D.; Laishley, E.J.

Anaerobe

1

61-67

439663

-

Purification and characterization of membrane-bound hydrogenase from Methanosarcina barkeri MS

1994

Kemner, J.M.; Zeikus, J.G.

Arch. Microbiol.

161

47-54

439664

8389482

Hydrogenase of the hyperthermophile Pyrococcus furiosus is an elemental sulfur reductase or sulfhydrogenase: Evidence for a sulfur-reducing hydrogenase ancestor

1993

Ma, K.; Schicho, R.N.; Kelly, R.M.; Adams, M.W.W.

Proc. Natl. Acad. Sci. USA

90

5341-5344

439665

8513797

Isolation, characterization and N-terminal amino acid sequence of hydrogenase from the green alga Chlamydomonas reinhardtii

1993

Happe, T.; Naber, J.D.

Eur. J. Biochem.

214

475-481

439668

3038877

An investigation of hydrogenase I and hydrogenase II from Clostridium pasteurianum by resonance Raman spectroscopy. Evidence for a [2Fe-2S] cluster in hydrogenase I

1987

Macor, K.A.; Czernuszewicz, R.S.; Adams, M.W.W.; Spiro, T.G.

J. Biol. Chem.

262

9945-9947

439669

6327705

The physical and catalytic properties of hydrogenase II of Clostridium pasteurianum. A comparison with hydrogenase I

1984

Adams, M.W.W.; Mortenson, L.E.

J. Biol. Chem.

259

7045-7055

439670

-

The purification of hydrogenase II (uptake hydrogenase) from the anaerobic N2-fixing bacterium Clostridium pasteurianum

1984

Adams, M.W.W.; Mortenson, L.E.

Biochim. Biophys. Acta

766

51-61

439671

6331453

Purification and properties of the H2-oxidizing (uptake) hydrogenase of the N2-fixing anaerobe Clostridium pasteurianum W5

1984

Chen, J.S.; Blanchard, D.K.

Biochem. Biophys. Res. Commun.

122

9-16

439672

16663691

Purification of hydrogenase from Chlamydomonas reinhardtii

1984

Roessler, P.G.; Lien, S.

Plant Physiol.

75

705-709

439674

7011807

The effects of pH and redox potential on the hydrogen production activity of the hydrogenase from Megasphaera elsdenii

1981

Van Dijk, C.; Veeger, C.

Eur. J. Biochem.

114

209-219

439675

7020766

The effect of electron carriers and other ligands on oxygen stability of clostridial hydrogenase

1981

Khan, S.M.; Klibanov, A.M.; Kaplan, N.O.; Kamen, M.D.

Biochim. Biophys. Acta

659

457-465

439677

527582

Purification and properties of hydrogenase from Megasphaera elsdenii

1979

Van Dijk, C.; Mayhew, S.G.; Grande, H.J.; Veeger, C.

Eur. J. Biochem.

102

317-330

439678

111712

Chelating agents protect hydrogenase against oxygen inactivation

1979

Klibanov, A.M.; Kaplan, N.O.; Kamen, M.D.

Biochim. Biophys. Acta

547

411-416

439679

728150

Isolation and properties of a unidirectional H2-oxidizing hydrogenase from the strictly anaerobic N2-fixing bacterium Clostridium pasteurianum W5

1978

Chen, J.S.; Blanchard, D.K.

Biochem. Biophys. Res. Commun.

84

1144-1150

439681

28770

The kinetics of methyl viologen oxidation and reduction by the hydrogenase from Clostridium pasteurianum

1978

Erbes, D.L.; Burris, R.H.

Biochim. Biophys. Acta

525

45-54

439682

4433567

Purification and properties of hydrogenase from Clostridium pasteurianum W5

1974

Chen, J.S.; Mortenson, L.E.

Biochim. Biophys. Acta

371

283-298

439684

4326766

Structural properties of hydrogenase from Clostridium pasteurianum W5

1971

Nakos, G.; Mortenson, L.E.

Biochemistry

10

2442-2449

439686

9485416

Purification and characterization of the HndA subunit of NADP-reducing hydrogenase from Desulfovibrio fructosovorans overproduced in Escherichia coli

1998

De Luca, G.; Asso, M.; Belaiech, J.P.; Dermoun, Z.

Biochemistry

37

2660-2665

439687

9703971

The NADP-reducing hydrogenase of Desulfovibrio fructosovorans: evidence for a native complex with hydrogen-dependent methyl-viologen-reducing activity

1998

De Luca, G.; De Philip, P.; Rousset, M.; Belaich, J.P.; Dermoun, Z.

Biochem. Biophys. Res. Commun.

248

591-596

439688

12460679

The NADP-reducing hydrogenase from Desulfovibrio fructosovorans: functional interaction between the C-terminal region of HndA and the N-terminal region of HndD subunits

2002

Dermoun, Z.; De Luca, G.; Asso, M.; Bertrand, P.; Guerlesquin, F.; Guigliarelli, B.

Biochim. Biophys. Acta

1556

217-225

439690

7751270

Characterization of an operon encoding an NADP-reducing hydrogenase in Desulfovibrio fructosovorans

1995

Malki, S.; Saimmaime, I.; De Luca, G.; Rousset, M.; Dermoun, Z.; Belaich, J.P.

J. Bacteriol.

177

2628-2636

439691

11115639

Electron pathways involved in H2-metabolism in the green alga Scenedesmus obliquus

2001

Wunschiers, R.; Senger, H.; Schulz, R.

Biochim. Biophys. Acta

1503

271-278

439692

2188945

Cloning and nucleotide sequences of the genes for the subunits of NAD-reducing hydrogenase of Alcaligenes eutrophus H16

1990

Tran-Betcke, A.; Warnecke, U.; Böcker, C.; Zaborosch, C.; Friedrich, B.

J. Bacteriol.

172

2920-2929

439693

4394007

Properties of the NAD-specific hydrogenase from Hydrogenomonas H 16

1970

Pfitzner, J.; Linke, H.A.B.; Schlegel, H.G.

Arch. Mikrobiol.

71

67-78

439694

-

Immunocytochemical localization of the soluble NAD-dependent hydrogenase in cells of alcaligenes eutrophus

1986

Rohde, M.; Johannssen, W.; Mayer, F.

FEMS Microbiol. Lett.

36

83-86

439695

3052436

Reversible and irreversible effects of nitric oxide on the soluble hydrogenase from Alcaligenes eutrophus H16

1988

Hyman, M.R.; Arp, D.J.

Biochem. J.

254

469-475

439696

3052435

Role of hydrogen in the activation and regulation of hydrogen oxidation by the soluble hydrogenase from Alcaligenes eutrophus H16

1988

Hyman, M.R.; Fox, C.A.; Arp, D.J.

Biochem. J.

254

463-468

439697

-

Inactivation of the NAD-dependent hydrogenase from the hydrogen-oxidizing bacterium Alcaligenes eutrophus Z1: Thermoinactivation mechanism

1985

Popov, V.O.; Ovchinnikov, A.N.; Utkin, I.B.; Gazaryan, I.G.; Egorov, A.M.; Berezin, I.V.

Biochim. Biophys. Acta

831

297-301

439698

-

NAD-dependent hydrogenase from the hydrogen-oxidizing bacterium Alcaligenes eutrophum Z1. Kinetic studies of the NADH-dehydrogenase activity

1985

Popov, V.O.; Gazaryan, I.G.; Egorov, A.M.; Berezin, I.V.

Biochim. Biophys. Acta

827

466-471

439699

186126

Purification and properties of soluble hydrogenase from Alcaligenes eutrophus H 16

1976

Schneider, K.; Schlegel, H.G.

Biochim. Biophys. Acta

452

66-80

439700

871226

Localization and stability of hydrogenases from aerobic hydrogen bacteria

1977

Schneider, K.; Schlegel, H.G.

Arch. Microbiol.

112

229-238

439701

4374147

Studies on a gram-positive hydrogen bacterium, Nocardia opaca 1 b. III. Purification, stability and some properties of the soluble hydrogen dehydrogenase

1974

Aggag, M.; Schlegel, H.G.

Arch. Microbiol.

100

25-39

439702

-

Effect of nickel on activity and subunit composition of purified hydrogenase from Nocardia opaca 1 b

1984

Schneider, K.; Schlegel, H.G.; Jochim, K.

Eur. J. Biochem.

138

553-541

439703

6086343

Content and localization of FMN, Fe-S clusters and nickel in the NAD-linked hydrogenase of Nocardia opaca 1b

1984

Schneider, K.; Cammack, R.; Schlegel, H.G.

Eur. J. Biochem.

142

75-84

439704

2643386

Redox-dependent inactivation of the NAD-dependent hydrogenase from Alcaligenes eutrophus Z1

1989

Petrov, R.R.; Utkin, I.B.; Popov, V.O.

Arch. Biochem. Biophys.

268

298-305

439705

2643385

Effect of redox potential on the activation of the NAD-dependent hydrogenase from Alcaligenes eutrophus Z1

1989

Petrov, R.R.; Utkin, I.B.; Popov, V.O.

Arch. Biochem. Biophys.

268

287-297

439706

-

Nickel and iron incoorporation into soluble hydrogenase of alcaligenes eutrophus

1984

Friedrich, C.G.; Suetin, S.; Lohmeyer, M.

Arch. Microbiol.

140

206-211

439707

6749813

Nickel in the catalytically active hydrogenase of Alcaligenes eutrophus

1982

Friedrich, C.G.; Schneider, K.; Friedrich, B.

J. Bacteriol.

152

42-48

439708

6272708

Production of superoxide radicals by soluble hydrogenase from Alcaligenes eutrophus H16

1981

Schneider, K.; Schlegel, H.G.

Biochem. J.

193

99-107

439709

226163

The iron-sulphur centres of soluble hydrogenase from Alcaligenes eutrophus

1979

Schneider, K.; Cammack, R.; Schlegel, H.G.; Hall, D.O.

Biochim. Biophys. Acta

578

445-461

439710

718699

Identification and quantitative determination of the flavin component of soluble hydrogenase from Alcaligenes eutrophus

1978

Schneider, K.; Schlegel, H.G.

Biochem. Biophys. Res. Commun.

84

564-571

439711

9042758

Location, catalytic activity, and subunit composition of NAD-reducing hydrogenases of some Alcaligenes strains and Rhodococcus opacus MR22

1997

Grzeszik, C.; Ross, K.; Schneider, K.; Reh, M.; Schlegel, H.G.

Arch. Microbiol.

167

172-176

439712

10682839

Unusual FTIR and EPR properties of the H2-activating site of the cytoplasmic NAD-reducing hydrogenase from Ralstonia eutropha

2000

Happe, R.P.; Roseboom, W.; Egert, G.; Friedrich, C.G.; Massanz, C.; Friedrich, B.; Albracht, S.P.

FEBS Lett.

466

259-263

439713

11807565

Expression of a functional NAD-reducing [NiFe] hydrogenase from the gram-positive Rhodococcus opacus in the gram-negative Ralstonia eutropha

2002

Porthun, A.; Bernhard, M.; Friedrich, B.

Arch. Microbiol.

177

159-166

440134

-

The bioenergetics of electron transport to nitrogenase

1987

Haaker, H.; Klugkist, J.

FEMS Microbiol. Rev.

46

57-71

440138

9860849

Nitrogenase of Azotobacter vinelandii: kinetic analysis of the Fe protein redox cycle

1998

Duyvis, M.G.; Wassink, H.; Haaker, H.

Biochemistry

37

17345-17354

440140

8360623

Oxygen inhibition of nitrogenase activity in Klebsiella pneumoniae

1992

Kavanagh, E.P.; Hill, S.

J. Gen. Microbiol.

139

1307-1314

440142

3913292

Hydrogenase, nitrogenase, and hydrogen metabolism in the photosynthetic bacteria

1985

Vignais, P.M.; Colbeau, A.; Willison, J.C.; Jouanneau, Y.

Adv. Microb. Physiol.

26

155-234

440143

-

Isolation and characterization of various nitrogenases

1980

Eady, R.R.

Methods Enzymol.

69

753-778

440144

183600

Nitrogenase

1976

Winter, H.C.; Burris, R.H.

Annu. Rev. Biochem.

45

409-426

440145

4134899

Nitrogenase

1974

Eady, R.R.; Postgate, J.R.

Nature

249

805-810

440146

224803

Structure and function of nitrogenase

1979

Mortenson, L.E.; Thorneley, R.N.F.

Annu. Rev. Biochem.

48

387-418

440147

6383195

Molybdenum in nitrogenase

1984

Shah, V.K.; Ugalde, R.A.; Imperial, J.; Brill, W.J.

Annu. Rev. Biochem.

53

231-257

440148

-

The physiology and biochemistry of hydrogen metabolism in cyanobacteria

1984

Houchins, J.P.

Biochim. Biophys. Acta

768

227-255

440149

-

Iron-sulfur proteins

1975

Palmer, G.

The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )

12

1-56

440150

2672439

Nitrogenases without molybdenum

1989

Pau, R.N.

Trends Biochem. Sci.

14

183-186

440151

-

Coding properties of cloned nitrogenase structural genes from Rhizobium japonicum

1982

Fuhrmann, M.; Hennecke, H.

Mol. Gen. Genet.

187

419-425

440152

-

Cloning and characterization of nitrogenase genes from Anabaena variabilis

1985

Hirschberg, R.; Samson, S.M.; Kimmel, B.E.; Page, K.A.; Collins, J.J.; Myers, J.A.; Yarbrough, L.R.

J. Biotechnol.

2

23-37

440153

-

Crystallographic properties of the MoFe proteins of nitrogenase from Clostridium pasteurianum and Azotobacter vinelandii

1982

Weininger, M.S.; Mortenson, L.E.

Proc. Natl. Acad. Sci. USA

79

379-380

440154

4362294

Purification of nitrogenase and crystallization of its Mo-Fe protein

1972

Burns, R.C.; Hardy, R.W.F.

Methods Enzymol.

24

480-496

440155

2914845

Genetic evidence for an Azotobacter vinelandii nitrogenase lacking molybdenum and vanadium

1989

Pau, R.N.; Mitchenall, L.A.; Robson, R.L.

J. Bacteriol.

171

124-129

440156

3474027

Characterization of the metal clusters in the nitrogenase molybdenum-iron and vanadium-iron proteins of Azotobacter vinelandii using magnetic circular dichroism spectroscopy

1987

Morningstar, J.E.; Johnson, M.K.; Case, E.E.; Hales, B.J.

Biochemistry

26

1795-1800

440157

3512961

Isolation and characterization of nitrogenase from Klebsiella pneumoniae

1986

Shah, V.K.

Methods Enzymol.

118

511-519

440158

4670204

Nitrogenase complex and its components

1972

Bulen, W.A.; LeComte, J.R.

Methods Enzymol.

24B

456-470

440159

4670203

Purification aof nitrogenase from Clostridium pasteurianum

1972

Mortenson, L.E.

Methods Enzymol.

24B

446-456

440160

-

Properties of in vivo nitrogenase activity in Beggiatoa alba

1988

Polman, J.K.; Larkin, J.M.

Arch. Microbiol.

150

126-130

440161

3864779

Purification and properties of the nitrogenase of Azospirillum amazonense

1985

Song, S.D.; Hartmann, A.; Burris, R.H.

J. Bacteriol.

164

1271-1277

440162

-

Nitrogenase of Sesbania Rhizobium strain ORS571: purification, properties, and 'switch-off' by ammonia

1985

Kush, A.; Elmerich, C.; Aubert, J.P.

J. Gen. Microbiol.

131

1765-1777

440163

6930977

Large-scale purification of high activity Azotobacter vinelandii nitrogenase

1980

Burgess, B.K.; Jacobs, D.B.; Stiefel, E.I.

Biochim. Biophys. Acta

614

196-209

440164

4369192

Some properties of the nitrogenase proteins from Clostridium pasteurianum. Molecular weight, subunit structure, isoelectric point and EPR spectra

1974

Tso, M.Y.W.

Arch. Microbiol.

99

71-80

440165

4215454

Legume root nodule nitrogenase. Purification, properties, and studies on its genetic control

1974

Whitting, M.J.; Dilworth, M.J.

Biochim. Biophys. Acta

371

337-351

440166

-

Mechanismen zum Schutz der Sauerstoff-labilen Nitrogenase

1988

Oelze, J.

Forum Mikrobiol.

4

116-126

440167

173545

Nitrogenase from Azotobacter chroococcum. Purification and properties of the component proteins

1975

Yates, M.G.; Planque, K.

Eur. J. Biochem.

60

467-476

440168

207515

The nitrogen-fixing system of Corynebacterium autotrophicum. Purification and properties of the nitrogenase components and two ferredoxins

1978

Berndt, H.; Lowe, D.J.; Yates, M.G.

Eur. J. Biochem.

86

133-142

440169

213121

Nitrogenase from Bacillus polymyxa. Purification and properties of the component proteins

1978

Emerich, D.W.; Burris, R.H.

Biochim. Biophys. Acta

536

172-183

440170

111934

Purification and properties of nitrogenase from the cyanobacterium, Anabaena cylindrica

1979

Hallenbeck, P.C.; Kostel, P.J.; Benemann, J.R.

Eur. J. Biochem.

98

275-284

440171

6799495

Nitrogenase from the photosynthetic bacterium Rhodopseudomonas capsulata: purification and molecular properties

1982

Hallenbeck, P.C.; Meyer, C.; Vignais, P.M.

J. Bacteriol.

149

708-717

440174

164247

The nitrogen-fixing complex of bacteria

1975

Zumft, W.G.; Mortenson, L.E.

Biochim. Biophys. Acta

416

1-52

440175

7957225

Pre-steady-state MgATP-dependent proton production and electron transfer by nitrogenase from Azotobacter vinelandii

1994

Duyvis, M.G.; Wassink, H.; Haaker, H.

Eur. J. Biochem.

225

881-890

440176

9108249

Comparative biochemical characterization of the iron-only nitrogenase and the molybdenum nitrogenase from Rhodobacter capsulatus

1997

Schneider, K.; Gollan, U.; Drottboom, M.; Selsemeier-Voigt, S.; Muller, A.

Eur. J. Biochem.

244

789-800

440177

11895435

The Fe-only nitrogenase and the Mo nitrogenase from Rhodobacter capsulatus: a comparative study on the redox properties of the metal clusters present in the dinitrogenase components

2002

Siemann, S.; Schneider, K.; Drottboom, M.; Muller, A.

Eur. J. Biochem.

269

1650-1661

440178

9214303

Reduction of thiocyanate, cyanate, and carbon disulfide by nitrogenase: kinetic characterization and EPR spectroscopic analysis

1997

Rasche, M.E.; Seefeldt, L.C.

Biochemistry

36

8574-8585

440179

9730834

Catalytic and biophysical properties of a nitrogenase Apo-MoFe protein produced by a nifB-deletion mutant of Azotobacter vinelandii

1998

Christiansen, J.; Goodwin, P.J.; Lanzilotta, W.N.; Seefeldt, L.C.; Dean, D.R.

Biochemistry

37

12611-12623

440180

10572002

Enhanced efficiency of ATP hydrolysis during nitrogenase catalysis utilizing reductants That form the all-ferrous redox state of the Fe protein

1999

Erickson, J.A.; Nyborg, A.C.; Johnson, J.L.; Truscott, S.M.; Gunn, A.; Nordmeyer, F.R.; Watt, G.D.

Biochemistry

38

14279-14285

440181

10978172

Azotobacter vinelandii nitrogenases with substitutions in the FeMo-cofactor environment of the MoFe protein: effects of acetylene or ethylene on interactions with H+, HCN, and CN-

2000

Fisher, K.; Dilworth, M.J.; Kim, C.H.; Newton, W.E.

Biochemistry

39

10855-10865

440182

9642255

Characterization of a variant iron protein of nitrogenase that is impaired in its ability to adopt the MgATP-induced conformational change

1998

Bursey, E.H.; Burgess, B.K.

J. Biol. Chem.

273

16927-16934

440183

10753963

Isolation and characterization of an acetylene-resistant nitrogenase

2000

Christiansen, J.; Cash, V.L.; Seefeldt, L.C.; Dean, D.R.

J. Biol. Chem.

275

11459-11464

440184

9860864

Effects on substrate reduction of substitution of histidine-195 by glutamine in the alpha-subunit of the MoFe protein of Azotobacter vinelandii nitrogenase

1998

Dilworth, M.J.; Fisher, K.; Kim, C.H.; Newton, W.E.

Biochemistry

37

17495-17505

440186

8286368

Nitrogenase and biological nitrogen fixation

1994

Kim, J.; Rees, D.C.

Biochemistry

33

389-397

440187

8784188

Steady-state kinetic studies of dithionite utilization, component protein interaction, and the formation of an oxidized iron protein intermediate during Azotobacter vinelandii nitrogenase catalysis

1996

Johnson, J.L.; Tolley, A.M.; Erickson, J.A.; Watt, G.D.

Biochemistry

35

11336-11342

440188

8555217

Isolation of two forms of the nitrogenase VFe protein from Azotobacter vinelandii

1996

Blanchard, C.Z.; Hales, B.J.

Biochemistry

35

472-478

440189

7893691

Role of the MoFe protein alpha-subunit histidine-195 residue in FeMo-cofactor binding and nitrogenase catalysis

1995

Kim, C.H.; Newton, W.E.; Dean, D.R.

Biochemistry

34

2798-2808

440297

-

Effect of gamma-radiation on Methanosarcina hydrogenase containing transition metal ions

1990

Arnikar, H.J.; Bhosale, S.B.; Kshirsagar, D.C.; Kapadi, A.H.; Yeole, T.Y.

J. Radioanal. Nucl. Chem.

142

349-358

440299

9555882

Isolation and characterization of methanophenazine and function of phenazines in membrane-bound electron transport of Methanosarcina mazei Go1

1998

Abken, H.J.; Tietze, M.; Brodersen, J.; Baumer, S.; Beifuss, U.; Deppenmeier, U.

J. Bacteriol.

180

2027-2032

440300

10471795

Novel reactions involved in energy conservation by methanogenic archaea

1999

Deppenmeier, U.; Lienard, T.; Gottschalk, G.

FEBS Lett.

457

291-297

639391

2121478

Purification and properties of heterodisulfide reductase from Methanobacterium thermoautotrophicum (strain Marburg)

1990

Hedderich, R.; Berkessel, A.; Thauer, R.K.

Eur. J. Biochem.

193

255-261

654261

12039777

Uranium reduction by Desulfovibrio desulfuricans strain G20 and a cytochrome c3 mutant

2002

Payne, R.B.; Gentry, D.M.; Rapp-Giles, B.J.; Casalot, L.; Wall, J.D.

Appl. Environ. Microbiol.

68

3129-3132

654285

14766547

Cyanobacterial-type, heteropentameric, NAD+-reducing NiFe hydrogenase in the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina

2004

Rakhely, G.; Kovacs, A.T.; Maroti, G.; Fodor, B.D.; Csanadi, G.; Latinovics, D.; Kovacs, K.L.

Appl. Environ. Microbiol.

70

722-728

654385

-

H2:heterodisulfide oxidoreductase, a second energy-conserving system in the methanogenic strain Goe1

1991

Deppenmeier, U.; Blaut, M.; Gottschalk, G.

Arch. Microbiol.

155

272-277

654388

11807566

Detection and localization of two hydrogenases in Methylococcus capsulatus (Bath) and their potential role in methane metabolism

2002

Hanczar, T.; Csaki, R.; Bodrossy, L.; Murrell, J.C.; Kovacs, K.L.

Arch. Microbiol.

177

167-172

654562

15667255

Non-standard structures of the Ni-Fe cofactor in the regulatory and the NAD-reducing hydrogenases from Ralstonia eutropha

2005

Loescher, S.; Burgdorf, T.; Buhrke, T.; Friedrich, B.; Dau, H.; Haumann, M.

Biochem. Soc. Trans.

33

25-27

654782

14606942

Chemical modification of catalytically essential functional groups of NAD-dependent hydrogenase from Ralstonia eutropha H16

2003

Tikhonova, T.V.; Savel'eva, N.D.; Popov, V.O.

Biochemistry

68

994-1001

654821

12034472

HoxE - a subunit specific for the pentameric bidirectional hydrogenase complex (HoxEFUYH) of cyanobacteria

2002

Schmitz, O.; Boison, G.; Salzmann, H.; Bothe, H.; Schutz, K.; Wang, S.H.; Happe, T.

Biochim. Biophys. Acta

1554

66-74

654899

15780995

Interaction and electron transfer between the high molecular weight cytochrome and cytochrome c3 from Desulfovibrio vulgaris Hildenborough: kinetic, microcalorimetric, EPR and electrochemical studies

2005

Guiral, M.; Leroy, G.; Bianco, P.; Gallice, P.; Guigliarelli, B.; Bruschi, M.; Nitschke, W.; Giudici-Orticoni, M.T.

Biochim. Biophys. Acta

1723

45-54

655307

15696261

Biodiversity of hydrogenases in Frankia

2005

Leul, M.; Mohapatra, A.; Sellstedt, A.

Curr. Microbiol.

50

17-23

655314

-

Additional experiences with Proteus vulgaris and its hydroxycarboxylate-oxidoreductase for the preparation of (2R)- or (2S)-hydroxycarboxylates

1988

Krauss, F.; Guenther, H.; Skopan, H.; Simon, H.

DECHEMA Biotechnol. Conf.

1

313-318

655396

8477725

Purification of a cytochrome b containing H2:heterodisulfide oxidoreductase complex from membranes of Methanosarcina barkeri

1993

Heiden, S.; Hedderich, R.; Setzke, E.; Thauer, R.K.

Eur. J. Biochem.

213

529-535

655398

8119281

H2:heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum. Composition and properties

1994

Setzke, E.; Hedderich, R.; Heiden, S.; Thauer, R.K.

Eur. J. Biochem.

220

139-148

655400

8174566

Purification of a two-subunit cytochrome-b-containing heterodisulfide reductase from methanol-grown Methanosarcina barkeri

1994

Heiden, S.; Hedderich, R.; Setzke, E.; Thauer, R.K.

Eur. J. Biochem.

221

855-861

655446

12423371

Purification and catalytic properties of a CO-oxidizing:H2-evolving enzyme complex from Carboxydothermus hydrogenoformans

2002

Soboh, B.; Linder, D.; Hedderich, R.

Eur. J. Biochem.

269

5712-5721

655471

12492476

Bioenergetics of the formyl-methanofuran dehydrogenase and heterodisulfide reductase reactions in Methanothermobacter thermautotrophicus

2003

de Poorter, L.M.; Geerts, W.G.; Theuvenet, A.P.; Keltjens, J.T.

Eur. J. Biochem.

270

66-75

655531

-

Methanobacterium thermoautotrophicum contains a soluble enzyme system that specifically catalyzes the reduction of the heterodisulfide of coenzyme M and 7-mercaptoheptanoylthreonine phosphate with H2

1988

Hedderich, R.; Thauer, R.K.

FEBS Lett.

234

223-227

655638

15251202

A hydrogen-evolving enzyme is present in Frankia sp. R43

2004

Mohapatra, A.; Leul, M.; Mattsson, U.; Sellstedt, A.

FEMS Microbiol. Lett.

236

235-240

655825

10383977

Energy conservation by the H2:heterodisulfide oxidoreductase from Methanosarcina mazei Gö1: identification of two proton-translocating segments

1999

Ide, T.; Baumer, S.; Deppenmeier, U.

J. Bacteriol.

181

4076-4080

655890

15838039

The soluble NAD+-Reducing [NiFe]-hydrogenase from Ralstonia eutropha H16 consists of six subunits and can be specifically activated by NADPH

2005

Burgdorf, T.; van der Linden, E.; Bernhard, M.; Yin, Q.Y.; Back, J.W.; Hartog, A.F.; Muijsers, A.O.; de Koster, C.G.; Albracht, S.P.; Friedrich, B.

J. Bacteriol.

187

3122-3132

656298

15164270

The soluble [NiFe]-hydrogenase from Ralstonia eutropha contains four cyanides in its active site, one of which is responsible for the insensitivity towards oxygen

2004

Van der Linden, E.; Burgdorf, T.; Bernhard, M.; Bleijlevens, B.; Friedrich, B.; Albracht, S.P.

J. Biol. Inorg. Chem.

9

616-626

656734

15256587

A multisubunit membrane-bound [NiFe] hydrogenase and an NADH-dependent Fe-only hydrogenase in the fermenting bacterium Thermoanaerobacter tengcongensis

2004

Soboh, B.; Linder, D.; Hedderich, R.

Microbiology

150

2451-2463

657318

14705030

Molecular basis for redox-Bohr and cooperative effects in cytochrome c3 from Desulfovibrio desulfuricans ATCC 27774: crystallographic and modeling studies of oxidized and reduced high-resolution structures at pH 7.6

2004

Bento, I.; Matias, P.M.; Baptista, A.M.; da Costa, P.N.; van Dongen, W.M.; Saraiva, L.M.; Schneider, T.R.; Soares, C.M.; Carrondo, M.A.

Proteins

54

135-152

657579

11872465

Differential expression of methanogenesis genes of Methanothermobacter thermoautotrophicus (formerly Methanobacterium thermoautotrophicum) in pure culture and in cocultures with fatty acid-oxidizing syntrophs

2002

Luo, H.W.; Zhang, H.; Suzuki, T.; Hattori, S.; Kamagata, Y.

Appl. Environ. Microbiol.

68

1173-1179

657605

15746376

Characterization of hydrogenase and reductive dehalogenase activities of Dehalococcoides ethenogenes strain 195

2005

Nijenhuis, I.; Zinder, S.H.

Appl. Environ. Microbiol.

71

1664-1667

657622

12861426

Hydrogenases in sulfate-reducing bacteria function as chromate reductase

2003

Chardin, B.; Giudici-Orticoni, M.T.; De Luca, G.; Guigliarelli, B.; Bruschi, M.

Appl. Microbiol. Biotechnol.

63

315-321

657739

12804572

A novel membrane-bound Ech [NiFe] hydrogenase in Desulfovibrio gigas

2003

Rodrigues, R.; Valente, F.M.A.; Pereira, I.A.C.; Oliveira, S.; Rodrigues-Pousada, C.

Biochem. Biophys. Res. Commun.

306

366-375

657778

15474481

Molecular cloning, characterization, and overexpression of a novel [Fe]-hydrogenase isolated from a high rate of hydrogen producing Enterobacter cloacae IIT-BT 08

2004

Mishra, J.; Khurana, S.; Kumar, N.; Ghosh, A.K.; Das, D.

Biochem. Biophys. Res. Commun.

324

679-685

658040

14769015

Substrate interactions with nitrogenase: Fe versus Mo

2004

Seefeldt, L.C.; Dance, I.G.; Dean, D.R.

Biochemistry

43

1401-1409

658082

15157115

Reactions of H2, CO, and O2 with active [NiFe]-hydrogenase from Allochromatium vinosum. A stopped-flow infrared study

2004

George, S.J.; Kurkin, S.; Thorneley, R.N.; Albracht, S.P.

Biochemistry

43

6808-6819

658138

15000682

Properties of stable hydrogenase from the purple sulfur bacterium Lamprobacter modestohalophilus

2004

Zadvorny, O.A.; Zorin, N.A.; Gogotov, I.N.; Gorlenko, V.M.

Biochemistry

69

164-169

658635

14686932

UV-A/blue-light inactivation of the ‘metal-free’ hydrogenase (Hmd) from methanogenic archaea. The enzyme contains functional iron after all

2004

Lyon, E.J.; Shima, S.; Buurman, G.; Chowdhuri, S.; Batschauer, A.; Steinbach, K.; Thauer, R.K.

Eur. J. Biochem.

271

195-204

658902

14730999

X-ray absorption spectroscopy of selenate reductase

2004

Maher, M.J.; Santini, J.; Pickering, I.J.; Prince, R.C.; Macy, J.M.; George, G.N.

Inorg. Chem.

43

402-404

658966

14582997

Measurement and ab initio calculation of CSA/dipole-dipole cross-correlated relaxation provide insight into the mechanism of a H2-forming dehydrogenase

2003

Bartoschek, S.; Buurman, G.; Geierstanger, B.H.; Lapham, J.; Griesinger, C.

J. Am. Chem. Soc.

125

13308-13309

658972

12848556

Enzyme electrokinetics: electrochemical studies of the anaerobic interconversions between active and inactive states of Allochromatium vinosum [NiFe]-hydrogenase

2003

Jones, A.K.; Lamle, S.E.; Pershad, H.R.; Vincent, K.A.; Albracht, S.P.; Armstrong, F.A.

J. Am. Chem. Soc.

125

8505-8514

658978

15506791

Carbon monoxide as an intrinsic ligand to iron in the active site of the iron-sulfur-cluster-free hydrogenase H2-forming methylenetetrahydromethanopterin dehydrogenase as revealed by infrared spectroscopy

2004

Lyon, E.J.; Shima, S.; Boecher, R.; Thauer, R.K.; Grevels, F.W.; Bill, E.; Roseboom, W.; Albracht, S.P.J.

J. Am. Chem. Soc.

126

14239-14248

658987

15869280

The mechanism of activation of a [NiFe]-hydrogenase by electrons, hydrogen, and carbon monoxide

2005

Lamle, S.E.; Albracht, S.P.; Armstrong, F.A.

J. Am. Chem. Soc.

127

6595-6604

659019

12591889

Characterization of a cytosolic NiFe-hydrogenase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1

2003

Kanai, T.; Ito, S.; Imanaka, T.

J. Bacteriol.

185

1705-1711

659041

15126463

Identification of an uptake hydrogenase required for hydrogen-dependent reduction of Fe(III) and other electron acceptors by Geobacter sulfurreducens

2004

Coppi, M.V.; O'Neil, R.A.; Lovley, D.R.

J. Bacteriol.

186

3022-3028

659110

15168611

Energy-converting [NiFe] hydrogenases from archaea and extremophiles: ancestors of complex I

2004

Hedderich, R.

J. Bioenerg. Biomembr.

36

65-75

659310

14688251

A Glutamate is the essential proton transfer gate during the catalytic cycle of the [NiFe] hydrogenase

2004

Dementin, S.; Burlat, B.; De Lacey, A.L.; Pardo, A.; Adryanczyk-Perrier, G.; Guigliarelli, B.; Fernandez, V.M.; Rousset, M.

J. Biol. Chem.

279

10508-10513

659355

14576151

Characterization of the menaquinone reduction site in the diheme cytochrome b membrane anchor of Wolinella succinogenes NiFe-hydrogenase

2004

Gross, R.; Pisa, R.; Sanger, M.; Lancaster, C.R.; Simon, J.

J. Biol. Chem.

279

274-281

659520

12459907

Spectroscopic and kinetic characterization of active site mutants of Desulfovibrio fructosovoransNi-Fe hydrogenase

2003

DeLacey, A.L.; Fernandez, V.M.; Rousset, M.; Cavazza, C.; Hatchikian, E.C.

J. Biol. Inorg. Chem.

8

129-134

659523

12605255

Characterization of a [2Fe-2S] protein encoded in the iron-hydrogenase operon of Thermotoga maritima

2003

Pan, G.; Menon, A.L.; Adams, M.W.

J. Biol. Inorg. Chem.

8

469-474

659878

12949162

Membrane-bound hydrogenase and sulfur reductase of the hyperthermophilic and acidophilic archaeon Acidianus ambivalens

2003

Laska, S.; Lottspeich, F.; Kletzin, A.

Microbiology

149

2357-2371

659887

15528652

Characterization and transcriptional analysis of hupSLW in Gloeothece sp. ATCC 27152: an uptake hydrogenase from a unicellular cyanobacterium

2004

Oliveira, P.; Leitao, E.; Tamagnini, P.; Moradas-Ferreira, P.; Oxelfelt, F.

Microbiology

150

3647-3655

659894

15870477

Hydrogen concentrations in methane-forming cells probed by the ratios of reduced and oxidized coenzyme F420

2005

de Poorter, L.M.; Geerts, W.J.; Keltjens, J.T.

Microbiology

151

1697-1705

660222

12692334

Accumulation of ferrous iron in Chlamydomonas reinhardtii. Influence of CO2 and anaerobic induction of the reversible hydrogenase

2003

Semin, B.K.; Davletshina, L.N.; Novakova, A.A.; Kiseleva, T.Y.; Lanchinskaya, V.Y.; Aleksandrov, A.Y.; Seifulina, N.; Ivanov, II; Seibert, M.; Rubin, A.B.

Plant Physiol.

131

1756-1764

664270

16497155

Interplay of human tissue kallikrein 4 (hK4) with the plasminogen activation system: hK4 regulates the structure and functions of the urokinase-type plasminogen activator receptor (uPAR)

2006

Beaufort, N.; Debela, M.; Creutzburg, S.; Kellermann, J.; Bode, W.; Schmitt, M.; Pidard, D.; Magdolen, V.

Biol. Chem.

387

217-222

671432

16269797

Symbiotic hydrogenase activity in Bradyrhizobium sp. (Vigna) increases nitrogen content in Vigna unguiculata plants

2005

Baginsky, C.; Brito, B.; Imperial, J.; Ruiz-Argueeso, T.; Palacios, J.M.

Appl. Environ. Microbiol.

71

7536-7538

671937

15667266

Molecular characterization of uptake hydrogenase in Frankia

2005

Leul, M.; Mattsson, U.; Sellstedt, A.

Biochem. Soc. Trans.

33

64-66

672067

16981725

Bias from H2 cleavage to production and coordination changes at the Ni-Fe active site in the NAD+-reducing hydrogenase from Ralstonia eutropha

2006

Loescher, S.; Burgdorf, T.; Zebger, I.; Hildebrandt, P.; Dau, H.; Friedrich, B.; Haumann, M.

Biochemistry

45

11658-11665

672118

16460012

Redox interaction of cytochrome c3 with [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F

2006

Yahata, N.; Saitoh, T.; Takayama, Y.; Ozawa, K.; Ogata, H.; Higuchi, Y.; Akutsu, H.

Biochemistry

45

1653-1662

672279

16038606

Use of a reverse micelle system for study of oligomeric structure of NAD+-reducing hydrogenase from Ralstonia eutropha H16

2005

Tikhonova, T.V.; Kurkin, S.A.; Klyachko, N.L.; Popov, V.O.

Biochemistry

70

645-651

672282

17223791

Characterization of catalytic properties of hydrogenase isolated from the unicellular cyanobacterium Gloeocapsa alpicola CALU 743

2006

Serebryakova, L.T.; Sheremetieva, M.E.

Biochemistry

71

1370-1376

672638

15951385

An autocatalytic step in the reaction cycle of hydrogenase from Thiocapsa roseopersicina can explain the special characteristics of the enzyme reaction

2005

Osz, J.; Bagyinka, C.

Biophys. J.

89

1984-1989

672729

16132849

A simplified method for assay of hydrogenase activities of H2 evolution and uptake in Enterobacter aerogenes

2005

Ren, Y.; Xing, X.H.; Zhang, C.; Gou, Z.

Biotechnol. Lett.

27

1029-1033

673529

16156794

Assignment of the [4Fe-4S] clusters of Ech hydrogenase from Methanosarcina barkeri to individual subunits via the characterization of site-directed mutants

2005

Forzi, L.; Koch, J.; Guss, A.M.; Radosevich, C.G.; Metcalf, W.W.; Hedderich, R.

FEBS J.

272

4741-4753

674165

15643882

Structural and oxidation-state changes at its nonstandard Ni-Fe site during activation of the NAD-reducing hydrogenase from Ralstonia eutropha detected by X-ray absorption, EPR, and FTIR spectroscopy

2005

Burgdorf, T.; Loescher, S.; Liebisch, P.; Van der Linden, E.; Galander, M.; Lendzian, F.; Meyer-Klaucke, W.; Albracht, S.P.; Friedrich, B.; Dau, H.; Haumann, M.

J. Am. Chem. Soc.

127

576-592

674190

17177431

Electrochemical investigations of the interconversions between catalytic and inhibited states of the [FeFe]-hydrogenase from Desulfovibrio desulfuricans

2006

Parkin, A.; Cavazza, C.; Fontecilla-Camps, J.C.; Armstrong, F.A.

J. Am. Chem. Soc.

128

16808-16815

674448

15849358

Oxygen tolerance of the H2-sensing [NiFe] hydrogenase from Ralstonia eutropha H16 is based on limited access of oxygen to the active site

2005

Buhrke, T.; Lenz, O.; Krauss, N.; Friedrich, B.

J. Biol. Chem.

280

23791-23796

674514

16236714

A membrane-bound multienzyme, hydrogen-oxidizing, and sulfur-reducing complex from the hyperthermophilic bacterium Aquifex aeolicus

2005

Guiral, M.; Tron, P.; Aubert, C.; Gloter, A.; Iobbi-Nivol, C.; Giudici-Orticoni, M.T.

J. Biol. Chem.

280

42004-42015

674919

16187073

Hydrogenases in Desulfovibrio vulgaris Hildenborough: structural and physiologic characterisation of the membrane-bound [NiFeSe] hydrogenase

2005

Valente, F.M.; Oliveira, A.S.; Gnadt, N.; Pacheco, I.; Coelho, A.V.; Xavier, A.V.; Teixeira, M.; Soares, C.M.; Pereira, I.A.

J. Biol. Inorg. Chem.

10

667-682

674936

17082918

Characterization of a cyanobacterial-like uptake [NiFe] hydrogenase: EPR and FTIR spectroscopic studies of the enzyme from Acidithiobacillus ferrooxidans

2007

Schroeder, O.; Bleijlevens, B.; de Jongh, T.E.; Chen, Z.; Li, T.; Fischer, J.; Foerster, J.; Friedrich, C.G.; Bagley, K.A.; Albracht, S.P.; Lubitz, W.

J. Biol. Inorg. Chem.

12

212-233

675221

16550436

Studies on inhibition of transformation of 2,4,6-trinitrotoluene catalyzed by Fe-only hydrogenase from Clostridium acetobutylicum

2006

Kutty, R.; Bennett, G.N.

J. Ind. Microbiol. Biotechnol.

33

368-376

675360

16226767

The type I/type II cytochrome c3 complex: an electron transfer link in the hydrogen-sulfate reduction pathway

2005

Pieulle, L.; Morelli, X.; Gallice, P.; Lojou, E.; Barbier, P.; Czjzek, M.; Bianco, P.; Guerlesquin, F.; Hatchikian, E.C.

J. Mol. Biol.

354

73-90

675493

16645314

[NiFe]-hydrogenases of Ralstonia eutropha H16: modular enzymes for oxygen-tolerant biological hydrogen oxidation

2005

Burgdorf, T.; Lenz, O.; Buhrke, T.; van der Linden, E.; Jones, A.K.; Albracht, S.P.; Friedrich, B.

J. Mol. Microbiol. Biotechnol.

10

181-196

676044

9822828

Identification of histidine residues in Wolinella succinogenes hydrogenase that are essential for menaquinone reduction by H2

1998

Gross, R.; Simon, J.; Lancaster, R.D.; Kröger, A.

Mol. Microbiol.

30

639-646

684463

17150028

Hydrogenases and hydrogen photoproduction in oxygenic photosynthetic organisms

2007

Ghirardi, M.L.; Posewitz, M.C.; Maness, P.; Dubini, A.; Yu, J.; Seibert, M.

Annu. Rev. Plant Biol.

58

71-91

684509

17588123

Ferric iron reduction by Desulfovibrio vulgaris Hildenborough wild type and energy metabolism mutants

2007

Park, H.S.; Lin, S.; Voordouw, G.

Antonie van Leeuwenhoek

93

79-85

684596

17277963

Flow-FISH analysis and isolation of clostridial strains in an anaerobic semi-solid bio-hydrogen producing system by hydrogenase gene target

2007

Jen, C.J.; Chou, C.H.; Hsu, P.C.; Yu, S.J.; Chen, W.E.; Lay, J.J.; Huang, C.C.; Wen, F.S.

Appl. Microbiol. Biotechnol.

74

1126-1134

684608

17668201

Escherichia coli hydrogenase 3 is a reversible enzyme possessing hydrogen uptake and synthesis activities

2007

Maeda, T.; Sanchez-Torres, V.; Wood, T.K.

Appl. Microbiol. Biotechnol.

76

1035-1042

684643

18320190

Deletion of iscR stimulates recombinant clostridial Fe-Fe hydrogenase activity and H2-accumulation in Escherichia coli BL21(DE3)

2008

Akhtar, M.K.; Jones, P.R.

Appl. Microbiol. Biotechnol.

78

853-862

684651

18651140

Identification of an uptake hydrogenase for hydrogen-dependent dissimilatory azoreduction by Shewanella decolorationis S12

2008

Hong, Y.G.; Guo, J.; Sun, G.P.

Appl. Microbiol. Biotechnol.

80

517-524

685165

17508723

Diazene (HN=NH) is a substrate for nitrogenase: insights into the pathway of N2 reduction

2007

Barney, B.M.; McClead, J.; Lukoyanov, D.; Laryukhin, M.; Yang, T.C.; Dean, D.R.; Hoffman, B.M.; Seefeldt, L.C.

Biochemistry

46

6784-6794

685293

17922655

Measuring the pH dependence of hydrogenase activities

2007

Tsygankov, A.A.; Minakov, E.A.; Zorin, N.A.; Gosteva, K.S.; Voronin, O.G.; Karyakin, A.A.

Biochemistry

72

968-973

685324

17376400

The role of Hox hydrogenase in the H2 metabolism of Thiocapsa roseopersicina

2007

Rakhely, G.; Laurinavichene, T.V.; Tsygankov, A.A.; Kovacs, K.L.

Biochim. Biophys. Acta

1767

671-676

685620

17660315

Atomic resolution modeling of the ferredoxin:[FeFe] hydrogenase complex from Chlamydomonas reinhardtii

2007

Chang, C.H.; King, P.W.; Ghirardi, M.L.; Kim, K.

Biophys. J.

93

3034-3045

685627

18621810

Brownian dynamics and molecular dynamics study of the association between hydrogenase and ferredoxin from Chlamydomonas reinhardtii

2008

Long, H.; Chang, C.H.; King, P.W.; Ghirardi, M.L.; Kim, K.

Biophys. J.

95

3753-3766

686267

18074176

Characterization of two 2[4Fe4S] ferredoxins from Clostridium acetobutylicum

2008

Guerrini, O.; Burlat, B.; Leger, C.; Guigliarelli, B.; Soucaille, P.; Girbal, L.

Curr. Microbiol.

56

261-267

686422

17888007

Hydrogenase- and outer membrane c-type cytochrome-facilitated reduction of technetium(VII) by Shewanella oneidensis MR-1

2008

Marshall, M.J.; Plymale, A.E.; Kennedy, D.W.; Shi, L.; Wang, Z.; Reed, S.B.; Dohnalkova, A.C.; Simonson, C.J.; Liu, C.; Saffarini, D.A.; Romine, M.F.; Zachara, J.M.; Beliaev, A.S.; Fredrickson, J.K.

Environ. Microbiol.

10

125-136

686732

17597615

The CO and CN(-) ligands to the active site Fe in [NiFe]-hydrogenase of Escherichia coli have different metabolic origins

2007

Forzi, L.; Hellwig, P.; Thauer, R.K.; Sawers, R.G.

FEBS Lett.

581

3317-3321

686733

17599837

Carbamoylphosphate serves as the source of CN(-), but not of the intrinsic CO in the active site of the regulatory [NiFe]-hydrogenase from Ralstonia eutropha

2007

Lenz, O.; Zebger, I.; Hamann, J.; Hildebrandt, P.; Friedrich, B.

FEBS Lett.

581

3322-3326

686734

17601576

The [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough is a bacterial lipoprotein lacking a typical lipoprotein signal peptide

2007

Valente, F.M.; Pereira, P.M.; Venceslau, S.S.; Regalla, M.; Coelho, A.V.; Pereira, I.A.

FEBS Lett.

581

3341-3344

686793

17681007

Complete activity profile of Clostridium acetobutylicum [FeFe]-hydrogenase and kinetic parameters for endogenous redox partners

2007

Demuez, M.; Cournac, L.; Guerrini, O.; Soucaille, P.; Girbal, L.

FEMS Microbiol. Lett.

275

113-121

687302

18205358

[FeFe]-hydrogenase-catalyzed H2 production in a photoelectrochemical biofuel cell

2008

Hambourger, M.; Gervaldo, M.; Svedruzic, D.; King, P.W.; Gust, D.; Ghirardi, M.; Moore, A.L.; Moore, T.A.

J. Am. Chem. Soc.

130

2015-2022

687403

18039761

An AbrB-like protein regulates the expression of the bidirectional hydrogenase in Synechocystis sp. strain PCC 6803

2008

Oliveira, P.; Lindblad, P.

J. Bacteriol.

190

1011-1019

687409

18156274

Reinvestigation of the steady-state kinetics and physiological function of the soluble NiFe-hydrogenase I of Pyrococcus furiosus

2008

van Haaster, D.J.; Silva, P.J.; Hagedoorn, P.L.; Jongejan, J.A.; Hagen, W.R.

J. Bacteriol.

190

1584-1587

687420

18487331

Roles of coenzyme F420-reducing hydrogenases and hydrogen- and F420-dependent methylenetetrahydromethanopterin dehydrogenases in reduction of F420 and production of hydrogen during methanogenesis

2008

Hendrickson, E.L.; Leigh, J.A.

J. Bacteriol.

190

4818-4821

687827

17924153

The exchange activities of [Fe] hydrogenase (iron-sulfur-cluster-free hydrogenase) from methanogenic archaea in comparison with the exchange activities of [FeFe] and [NiFe] hydrogenases

2008

Vogt, S.; Lyon, E.J.; Shima, S.; Thauer, R.K.

J. Biol. Inorg. Chem.

13

97-106

688655

-

Role of positive charge of lysine residue on cytochrome c3 for electrostatic interaction with hydrogenase

2007

Iida, S.; Asakura, N.; Tabata, K.; Okura, I.; Kamachi, T.

J. Porphyr. Phthalocyanines

11

66-73

688848

17321121

Purification and characterization of putative alkaline [Ni-Fe] hydrogenase from unicellular marine green alga, Tetraselmis kochinensis NCIM 1605

2007

Bhosale, S.H.; Pant, A.; Khan, M.I.

Microbiol. Res.

164

131-137

689932

17847093

Proton pathways in a [NiFe]-hydrogenase: A theoretical study

2008

Teixeira, V.H.; Soares, C.M.; Baptista, A.M.

Proteins

70

1010-1022

690013

17368855

Genes involved in Sec-independent membrane targeting of hydrogenase in Azotobacter chroococcum

2007

Maltempi de Souza, E.; de Oliveira Pedrosa, F.; Wassem, R.; Ford, C.M.; Yates, M.G.

Res. Microbiol.

158

272-278

690060

18653896

The crystal structure of [Fe]-hydrogenase reveals the geometry of the active site

2008

Shima, S.; Pilak, O.; Vogt, S.; Schick, M.; Stagni, M.S.; Meyer-Klaucke, W.; Warkentin, E.; Thauer, R.K.; Ermler, U.

Science

321

572-575

696971

19671178

Comprehensive computational analysis of Hmd enzymes and paralogs in methanogenic Archaea

2009

Goldman, A.; Leigh, J.; Samudrala, R.

BMC Evol. Biol.

9

199-210

701586

-

Enzymatic recovery of platinum (IV) from industrial wastewater using a biosulphidogenic hydrogenase

2008

Rashamuse, K.; Mutambanengwe, C.; Whiteley, C.

Afr. J. Biotechnol.

7

1087-1095

701778

18156331

Genetic diversity of hydrogen-producing bacteria in an acidophilic ethanol-H2-coproducing system, analyzed using the [Fe]-hydrogenase gene

2008

Xing, D.; Ren, N.; Rittmann, B.

Appl. Environ. Microbiol.

74

1232-1239

701780

18310429

Gene expression by the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough grown on an iron electrode under cathodic protection conditions

2008

Caffrey, S.M.; Park, H.S.; Been, J.; Gordon, P.; Sensen, C.W.; Voordouw, G.

Appl. Environ. Microbiol.

74

2404-2413

702218

18942856

Structural and biological analysis of the metal sites of Escherichia coli hydrogenase accessory protein HypB

2008

Dias, A.V.; Mulvihill, C.M.; Leach, M.R.; Pickering, I.J.; George, G.N.; Zamble, D.B.

Biochemistry

47

11981-11991

702254

19138102

An autocatalytic mechanism for NiFe-hydrogenase: reduction to Ni(I) followed by oxidative addition

2009

Lill, S.O.; Siegbahn, P.E.

Biochemistry

48

1056-1066

702307

19397274

The structure of the active site H-cluster of [FeFe] hydrogenase from the green alga Chlamydomonas reinhardtii studied by X-ray absorption spectroscopy

2009

Stripp, S.; Sanganas, O.; Happe, T.; Haumann, M.

Biochemistry

48

5042-5049

702707

19527657

Concentration-dependent front velocity of the autocatalytic hydrogenase reaction

2009

Bodo, G.; Branca, R.M.; Toth, A.; Horvath, D.; Bagyinka, C.

Biophys. J.

96

4976-4983

703746

19025569

Purification and biochemical characterization of a membrane-bound [NiFe]-hydrogenase from a hydrogen-oxidizing, lithotrophic bacterium, Hydrogenophaga sp. AH-24

2009

Yoon, K.S.; Sakai, Y.; Tsukada, N.; Fujisawa, K.; Nishihara, H.

FEMS Microbiol. Lett.

290

114-120

704012

18333613

Terminal hydride in [FeFe]-hydrogenase model has lower potential for H2 production than the isomeric bridging hydride

2008

Barton, B.E.; Rauchfuss, T.B.

Inorg. Chem.

47

2261-2263

704013

18307282

Refining the active site structure of iron-iron hydrogenase using computational infrared spectroscopy

2008

Tye, J.W.; Darensbourg, M.Y.; Hall, M.B.

Inorg. Chem.

47

2380-2388

704015

19580299

Theoretical study of dioxygen induced inhibition of [FeFe]-hydrogenase

2009

Stiebritz, M.T.; Reiher, M.

Inorg. Chem.

48

7127-7140

704016

19601586

Biomimetic model featuring the NH proton and bridging hydride related to a proposed intermediate in enzymatic H(2) production by Fe-only hydrogenase

2009

Chiang, M.H.; Liu, Y.C.; Yang, S.T.; Lee, G.H.

Inorg. Chem.

48

7604-7612

704162

18781742

The difference a Se makes? Oxygen-tolerant hydrogen production by the [NiFeSe]-hydrogenase from Desulfomicrobium baculatum

2008

Parkin, A.; Goldet, G.; Cavazza, C.; Fontecilla-Camps, J.C.; Armstrong, F.A.

J. Am. Chem. Soc.

130

13410-13416

704287

19233927

A molybdopterin oxidoreductase is involved in H2 oxidation in Desulfovibrio desulfuricans G20

2009

Li, X.; Luo, Q.; Wofford, N.Q.; Keller, K.L.; McInerney, M.J.; Wall, J.D.; Krumholz, L.R.

J. Bacteriol.

191

2675-2682

704309

19648238

Identification of the [FeFe]-hydrogenase responsible for hydrogen generation in Thermoanaerobacterium saccharolyticum and demonstration of increased ethanol yield via hydrogenase knockout

2009

Shaw, A.J.; Hogsett, D.A.; Lynd, L.R.

J. Bacteriol.

191

6457-6464

704489

19304663

Spectroscopic insights into the oxygen-tolerant membrane-associated [NiFe] hydrogenase of Ralstonia eutropha H16

2009

Saggu, M.; Zebger, I.; Ludwig, M.; Lenz, O.; Friedrich, B.; Hildebrandt, P.; Lendzian, F.

J. Biol. Chem.

284

16264-16276

704518

19017638

Concerted action of two novel auxiliary proteins in assembly of the active site in a membrane-bound [NiFe] hydrogenase

2009

Ludwig, M.; Schubert, T.; Zebger, I.; Wisitruangsakul, N.; Saggu, M.; Strack, A.; Lenz, O.; Hildebrandt, P.; Friedrich, B.

J. Biol. Chem.

284

2159-2168

704606

19801638

Overexpression, isolation and spectroscopic characterization of the bidirectional [NiFe]-hydrogenase from Synechocystis sp. PCC 6803

2009

Germer, F.; Zebger, I.; Saggu, M.; Lendzian, F.; Schulz, R.; Appel, J.

J. Biol. Chem.

284

36462-36472

704744

19716518

Purification and characterization of the oxygen-thermostable hydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum camini

2009

Nishimura, H.; Sako, Y.

J. Biosci. Bioeng.

108

299-303

705334

19317477

Hydrogenase/ferredoxin charge-transfer complexes: effect of hydrogenase mutations on the complex association

2009

Long, H.; King, P.W.; Ghirardi, M.L.; Kim, K.

J. Phys. Chem. A

113

4060-4067

706442

19855833

Tyrosine, cysteine, and S-adenosyl methionine stimulate in vitro [FeFe] hydrogenase activation

2009

Kuchenreuther, J.M.; Stapleton, J.A.; Swartz, J.R.

PLoS ONE

4

e7565

706499

18511566

Thiolate-bridged dinuclear iron(tris-carbonyl)-nickel complexes relevant to the active site of [NiFe] hydrogenase

2008

Ohki, Y.; Yasumura, K.; Kuge, K.; Tanino, S.; Ando, M.; Li, Z.; Tatsumi, K.

Proc. Natl. Acad. Sci. USA

105

7652-7657

710927

19114523

Role of the Hya hydrogenase in recycling of anaerobically produced H2 in Salmonella enterica serovar Typhimurium

2009

Zbell, A.; Maier, R.

Appl. Environ. Microbiol.

75

1456-1459

710951

21257809

A [NiFe]-hydrogenase from Alteromonas macleodii with unusual stability in the presence of oxygen and high temperature

2011

Vargas, W.A.; Weyman, P.D.; Tong, Y.; Smith, H.O.; Xu, Q.

Appl. Environ. Microbiol.

77

1990-1998

711266

20886839

Redox linked conformational changes in cytochrome c3 from Desulfovibrio desulfuricans ATCC 27774

2010

Paixao, V.B.; Vis, H.; Turner, D.L.

Biochemistry

49

9620-9629

711516

20699572

Purification and characterization of membrane-associated hydrogenase from the deep-sea epsilonproteobacterium Hydrogenimonas thermophila

2010

Nishimura, H.; Kitano, Y.; Inoue, T.; Nomura, K.; Sako, Y.

Biosci. Biotechnol. Biochem.

74

1624-1630

711862

20050876

Streptomycetes contributing to atmospheric molecular hydrogen soil uptake are widespread and encode a putative high-affinity [NiFe]-hydrogenase

2010

Constant, P.; Chowdhury, S.P.; Pratscher, J.; Conrad, R.

Environ. Microbiol.

12

821-829

712029

19222582

Immunolocalization of the uptake hydrogenase in the marine cyanobacterium Lyngbya majuscula CCAP 1446/4 and two Nostoc strains

2009

Seabra, R.; Santos, A.; Pereira, S.; Moradas-Ferreira, P.; Tamagnini, P.

FEMS Microbiol. Lett.

292

57-62

712281

19411328

The iron-hydrogenase of Thermotoga maritima utilizes ferredoxin and NADH synergistically: a new perspective on anaerobic hydrogen production

2009

Schut, G.J.; Adams, M.W.

J. Bacteriol.

191

4451-4457

712301

20511510

Characterization of energy-conserving hydrogenase B in Methanococcus maripaludis

2010

Major, T.A.; Liu, Y.; Whitman, W.B.

J. Bacteriol.

192

4022-4030

712307

19948802

Function of Ech hydrogenase in ferredoxin-dependent, membrane-bound electron transport in Methanosarcina mazei

2010

Welte, C.; Kallnik, V.; Grapp, M.; Bender, G.; Ragsdale, S.; Deppenmeier, U.

J. Bacteriol.

192

674-678

713368

20838423

Symbiotic legume nodules employ both rhizobial exo- and endo-hydrogenases to recycle hydrogen produced by nitrogen fixation

2010

Ciccolella, C.O.; Raynard, N.A.; Mei, J.H.; Church, D.C.; Ludwig, R.A.

PLoS ONE

5

e12094

713374

21079771

Distribution analysis of hydrogenases in surface waters of marine and freshwater environments

2010

Barz, M.; Beimgraben, C.; Staller, T.; Germer, F.; Opitz, F.; Marquardt, C.; Schwarz, C.; Gutekunst, K.; Vanselow, K.H.; Schmitz, R.; LaRoche, J.; Schulz, R.; Appel, J.

PLoS ONE

5

e13846

713401

19934053

A kinetic and thermodynamic understanding of O2 tolerance in [NiFe]-hydrogenases

2009

Cracknell, J.A.; Wait, A.F.; Lenz, O.; Friedrich, B.; Armstrong, F.A.

Proc. Natl. Acad. Sci. USA

106

20681-20686

722289

-

Hydrogen production from pyruvate by enzymes purified from the hyperthermophilic archaeon, Pyrococcus furiosus: A key role for NADPH

1994

Ma, K.; Hao, Z.; Adams, M.

FEMS Microbiol. Lett.

122

245-250

722508

12446645

Characterization of a fourth tungsten-containing enzyme from the hyperthermophilic archaeon Pyrococcus furiosus

2002

Roy, R.; Adams, M.W.

J. Bacteriol.

184

6952-6956

724456

23385167

Electron transfer between multihaem cytochromes c3 from Desulfovibrio africanus

2013

Quintas, P.O.; Oliveira, M.S.; Catarino, T.; Turner, D.L.

Biochim. Biophys. Acta

1827

502-506

725206

22540997

Understanding and tuning the catalytic bias of hydrogenase

2012

Abou Hamdan, A.; Dementin, S.; Liebgott, P.P.; Gutierrez-Sanz, O.; Richaud, P.; De Lacey, A.L.; Rousset, M.; Bertrand, P.; Cournac, L.; Leger, C.

J. Am. Chem. Soc.

134

8368-8371

726282

22016788

Catalytic properties of the isolated diaphorase fragment of the NAD +-reducing [NiFe]-hydrogenase from Ralstonia eutropha

2011

Lauterbach, L.; Idris, Z.; Vincent, K.; Lenz, O.

PLoS ONE

6

e25939

726344

23861944

An innovative cloning platform enables large-scale production and maturation of an oxygen-tolerant [NiFe]-hydrogenase from Cupriavidus necator in Escherichia coli

2013

Schiffels, J.; Pinkenburg, O.; Schelden, M.; Aboulnaga, e.l.-.H.A.; Baumann, M.E.; Selmer, T.

PLoS ONE

8

e68812

726376

21262829

Coupling of ferredoxin and heterodisulfide reduction via electron bifurcation in hydrogenotrophic methanogenic archaea

2011

Kaster, A.; Moll, J.; Parey, K.; Thauer, R.

Proc. Natl. Acad. Sci. USA

108

2981-2986

726704

16349220

Pressure stabilization of proteins from extreme thermophiles

1994

Hei, D.J.; Clark, D.S.

Appl. Environ. Microbiol.

60

932-939

727399

2550229

Purification of the F420-reducing hydrogenase from Methanosarcina barkeri (strain Fusaro)

1989

Fiebig, K.; Friedrich, B.

Eur. J. Biochem.

184

79-88

727402

1521540

H2-forming methylenetetrahydromethanopterin dehydrogenase, a novel type of hydrogenase without iron-sulfur clusters in methanogenic archaea

1992

Zirngibl, C.; Van Dongen, W.; Schwörer, B.; Von Bünau, R.; Richter, M.; Klein, A.; Thauer, R.K.

Eur. J. Biochem.

208

511-520

727746

17693508

Cwp84, a surface-associated protein of Clostridium difficile, is a cysteine protease with degrading activity on extracellular matrix proteins

2007

Janoir, C.; Pechine, S.; Grosdidier, C.; Collignon, A.

J. Bacteriol.

189

7174-7180

727829

10751389

The F420H2 dehydrogenase from Methanosarcina mazei is a Redox-driven proton pump closely related to NADH dehydrogenases

2000

Baumer, S.; Ide, T.; Jacobi, C.; Johann, A.; Gottschalk, G.; Deppenmeier, U.

J. Biol. Chem.

275

17968-17973

730867

25476267

Engineering the respiratory membrane-bound hydrogenase of the hyperthermophilic archaeon Pyrococcus furiosus and characterization of the catalytically active cytoplasmic subcomplex

2015

McTernan, P.M.; Chandrayan, S.K.; Wu, C.H.; Vaccaro, B.J.; Lancaster, W.A.; Adams, M.W.

Protein Eng. Des. Sel.

28

1-8

730884

24613729

Yeast 3,5-bisphosphate nucleotidase: an affinity tag for protein purification

2014

Yang, Y.; Ma, J.; Yang, Y.; Zhang, X.; Wang, Y.; Yang, L.; Sun, M.

Protein Expr. Purif.

97

81-87

734087

23893107

NADP-specific electron-bifurcating [FeFe]-hydrogenase in a functional complex with formate dehydrogenase in Clostridium autoethanogenum grown on CO

2013

Wang, S.; Huang, H.; Kahnt, H.; Mueller, A.; Köpke, M.; Thauer, R.

J. Bacteriol.

195

4373-4386

740618

23316038

A reversible electron-bifurcating ferredoxin- and NAD-dependent [FeFe]-hydrogenase (HydABC) in Moorella thermoacetica

2013

Wang, S.; Huang, H.; Kahnt, J.; Thauer, R.K.

J. Bacteriol.

195

1267-1275

740625

25157086

Hydrogen formation and its regulation in Ruminococcus albus: Involvement of an electron-bifurcating [FeFe]-hydrogenase, of a non-electron-bifurcating [FeFe]-hydrogenase, and of a putative hydrogen-sensing [FeFe]-hydrogenase

2014

Zheng, Y.; Kahnt, J.; Kwon, I.H.; Mackie, R.I.; Thauer, R.K.

J. Bacteriol.

196

3840-3852

741931

27396836

Importance of hydrogen bonding in fine tuning the [2Fe-2S] cluster redox potential of HydC from Thermotoga maritima

2016

Birrell, J.A.; Laurich, C.; Reijerse, E.J.; Ogata, H.; Lubitz, W.

Biochemistry

55

4344-4355

741956

24333786

Bioenergetics and anaerobic respiratory chains of aceticlastic methanogens

2014

Welte, C.; Deppenmeier, U.

Biochim. Biophys. Acta

1837

1130-1147

742006

28192203

Electrostatic roles in electron transfer from [NiFe] hydrogenase to cytochrome c3 from Desulfovibrio vulgaris Miyazaki F

2017

Sugimoto, Y.; Kitazumi, Y.; Shirai, O.; Nishikawa, K.; Higuchi, Y.; Yamamoto, M.; Kano, K.

Biochim. Biophys. Acta

1865

481-487

742265

27055899

Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

2016

Flanagan, L.A.; Wright, J.J.; Roessler, M.M.; Moir, J.W.; Parkin, A.

Chem. Commun. (Camb.)

52

9133-9136

742279

26010059

Structural insight into the complex of ferredoxin and [FeFe] Hydrogenase from Chlamydomonas reinhardtii

2015

Rumpel, S.; Siebel, J.F.; Diallo, M.; Fares, C.; Reijerse, E.J.; Lubitz, W.

ChemBioChem

16

1663-1669

742368

24569482

Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector

2014

Allegretti, M.; Mills, D.J.; McMullan, G.; Kuehlbrandt, W.; Vonck, J.

eLife

3

e01963

742373

26339289

Solar powered biohydrogen production requires specific localization of the hydrogenase

2014

Burroughs, N.J.; Boehm, M.; Eckert, C.; Mastroianni, G.; Spence, E.M.; Yu, J.; Nixon, P.J.; Appel, J.; Mullineaux, C.W.; Bryan, S.J.

Energy Environ. Sci.

7

3791-3800

742575

28083017

Distribution of hydrogenases in Cyanobacteria a phylum-wide genomic survey

2016

Puggioni, V.; Tempel, S.; Latifi, A.

Front. Genet.

7

223

742652

26359661

DFT dissection of the reduction step in H2 catalytic production by [FeFe]-hydrogenase-inspired models can the bridging hydride become more reactive than the terminal isomer?

2015

Filippi, G.; Arrigoni, F.; Bertini, L.; De Gioia, L.; Zampella, G.

Inorg. Chem.

54

9529-9542

742695

-

Improved oxygen tolerance of the Synechocystis sp. PCC 6803 bidirectional hydrogenase by site-directed mutagenesis of putative residues of the gas diffusion channel

2014

Cano, M.; Volbeda, A.; Guedeney, G.; Aubert-Jousset, E.; Richaud, P.; Peltier, G.; Cournac, L.

Int. J. Hydrogen Energy

39

16872-16884

742696

-

Catalytic cycle of cytochrome-c3 hydrogenase, a [NiFe]-enzyme, deduced from the structures of the enzyme and the enzyme mimic

2014

Yagi, T.; Ogo, S.; Higuchi, Y.

Int. J. Hydrogen Energy

39

18543-18550

742698

-

The ferredoxin Rr-HydB is required for the H2-evolving activity of Rr-HydA, a [FeFe]-hydrogenase of Rhodospirillum rubrum

2015

Kim, E.; Tong, X.; Lee, J.

Int. J. Hydrogen Energy

40

4320-4328

742740

25136926

Nitrite and hydroxylamine as nitrogenase substrates mechanistic implications for the pathway of N2 reduction

2014

Shaw, S.; Lukoyanov, D.; Danyal, K.; Dean, D.R.; Hoffman, B.M.; Seefeldt, L.C.

J. Am. Chem. Soc.

136

12776-12783

742784

25157086

Hydrogen formation and its regulation in Ruminococcus albus involvement of an electron-bifurcating [FeFe]-hydrogenase, of a non-electron-bifurcating [FeFe]-hydrogenase, and of a putative hydrogen-sensing [FeFe]-hydrogenase

2014

Zheng, Y.; Kahnt, J.; Kwon, I.H.; Mackie, R.I.; Thauer, R.K.

J. Bacteriol.

196

3840-3852

742788

25368299

Physiology and bioenergetics of [NiFe]-hydrogenase 2-catalyzed H2-consuming and H2-producing reactions in Escherichia coli

2015

Pinske, C.; Jaroschinsky, M.; Linek, S.; Kelly, C.L.; Sargent, F.; Sawers, R.G.

J. Bacteriol.

197

296-306

742858

24311779

The bidirectional NiFe-hydrogenase in Synechocystis sp. PCC 6803 is reduced by flavodoxin and ferredoxin and is essential under mixotrophic, nitrate-limiting conditions

2014

Gutekunst, K.; Chen, X.; Schreiber, K.; Kaspar, U.; Makam, S.; Appel, J.

J. Biol. Chem.

289

1930-1937

742859

24860091

Intact functional fourteen-subunit respiratory membrane-bound [NiFe]-hydrogenase complex of the hyperthermophilic archaeon Pyrococcus furiosus

2014

McTernan, P.M.; Chandrayan, S.K.; Wu, C.H.; Vaccaro, B.J.; Lancaster, W.A.; Yang, Q.; Fu, D.; Hura, G.L.; Tainer, J.A.; Adams, M.W.

J. Biol. Chem.

289

19364-19372

743086

24887099

The F420-reducing [NiFe]-hydrogenase complex from Methanothermobacter marburgensis, the first X-ray structure of a group 3 family member

2014

Vitt, S.; Ma, K.; Warkentin, E.; Moll, J.; Pierik, A.J.; Shima, S.; Ermler, U.

J. Mol. Biol.

426

2813-2826

743382

23345541

Morphological development and cytochrome c oxidase activity in Streptomyces lividans are dependent on the action of a copper bound Sco protein

2013

Blundell, K.L.; Wilson, M.T.; Svistunenko, D.A.; Vijgenboom, E.; Worrall, J.A.

Open Biology

3

120163

743732

25462812

High yield purification of a tagged cytoplasmic [NiFe]-hydrogenase and a catalytically-active nickel-free intermediate form

2015

Chandrayan, S.K.; Wu, C.H.; McTernan, P.M.; Adams, M.W.

Protein Expr. Purif.

107

90-94

743797

26215212

[NiFe]-hydrogenase is essential for cyanobacterium Synechocystis sp. PCC 6803 aerobic growth in the dark

2015

De Rosa, E.; Checchetto, V.; Franchin, C.; Bergantino, E.; Berto, P.; Szabo, I.; Giacometti, G.M.; Arrigoni, G.; Costantini, P.

Sci. Rep.

5

12424

744179

12856108

Physiological role of the F420-non-reducing hydrogenase (MvH) from Methanothermobacter marburgensis

2003

Stojanowic, A.; Mander, G.; Duin, E.; Hedderich, R.

Arch. Microbiol.

180

194-203

744833

8119281

H2 heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum Composition and properties

1994

Setzke, E.; Hedderich, R.; Heiden, S.; Thauer, R.

Eur. J. Biochem.

220

139-148

744926

-

Hydrogenase from the thermophilic bacterium Thermococcus stetteri Isolation and characterisation of EPR-detectable redox centres

1996

Zorin, N.; Medina, M.; Pusheva, M.; Gogotov, I.; Cammack, R.

FEMS Microbiol. Lett.

142

71-76

745388

27928630

Production and isolation of vanadium nitrogenase from Azotobacter vinelandii by molybdenum depletion

2017

Sippel, D.; Schlesier, J.; Rohde, M.; Trncik, C.; Decamps, L.; Djurdjevic, I.; Spatzal, T.; Andrade, S.L.; Einsle, O.

J. Biol. Inorg. Chem.

22

161-168

746492

20689010

Vanadium nitrogenase reduces CO

2010

Lee, C.; Hu, Y.; Ribbe, M.

Science

329

642

754980

26682577

Angiostrongylus cantonensis cathepsin B-like protease (Ac-cathB-1) is involved in host gut penetration

2015

Long, Y.; Cao, B.; Yu, L.; Tukayo, M.; Feng, C.; Wang, Y.; Luo, D.

Parasite

22

37