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Ligand H2

Basic Ligand Information

Molecular Structure

Pathway Source

Pathways

BRENDA

coenzyme M biosynthesis, hydrogen production, methanogenesis from CO2, nitrate assimilation, sulfate reduction

KEGG

Carbohydrate digestion and absorption, Protein digestion and absorption, Two-component system

MetaCyc

(S)-lactate fermentation to propanoate, acetate and hydrogen, coenzyme B/coenzyme M regeneration I (methanophenazine-dependent), coenzyme B/coenzyme M regeneration IV (H2-dependent), hydrogen oxidation I (aerobic), hydrogen oxidation II (aerobic, NAD), hydrogen oxidation III (anaerobic, NADP), hydrogen production I, hydrogen production II, hydrogen production III, hydrogen production IV, hydrogen production V, hydrogen production VI, hydrogen production VIII, hydrogen to dimethyl sulfoxide electron transfer, hydrogen to fumarate electron transfer, hydrogen to trimethylamine N-oxide electron transfer, L-glutamate degradation VII (to butanoate), methanogenesis from H2 and CO2, mixed acid fermentation, nitrogen fixation I (ferredoxin), nitrogen fixation II (flavodoxin), reductive acetyl coenzyme A pathway II (autotrophic methanogens), sulfur reduction I, sulfur reduction II (via polysulfide), superpathway of fermentation (Chlamydomonas reinhardtii) more

Show all BRENDA pathways known for H2

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (23 results)

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Show Natural Substrate (23 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.12.1.2

H2 + NAD+ = H+ + NADH

439633, 656298, 742695, 742373, 742858, 743797, 656734, 654821, 742575, 439692, 439694, 654388, 654562, 655638, 654285, 655890, 655307, 672067

-

1.12.1.2

H2 + oxidized methyl viologen = H+ + reduced methyl viologen

655638

-

1.12.1.3

H2 + NADP+ = H+ + NADPH

704606, 439687, 687409

-

1.12.1.3

H2 + oxidized ferredoxin = H+ + reduced ferredoxin

439691

-

1.12.1.2

2 entries

1.12.1.3

2 entries

1.12.1.4

H2 + NAD+ + oxidized ferredoxin = H+ + NADH + reduced ferredoxin

741931

-

1.12.2.1

H2 + ferricytochrome c3 = H+ + ferrocytochrome c3

674919, 742006, 742696, 654899, 675360

-

1.12.7.2

H2 + oxidized ferredoxin = H+ + reduced ferredoxin

439643, 439665, 439672, 439632, 439669, 439671, 439675, 439679, 439682, 439677, 712301, 439654, 439650

-

1.12.7.2

H2 + oxidized ferredoxin = reduced ferredoxin + H+

660222, 742859, 742784, 742698, 659110

-

1.12.7.2

H2 + oxidized ferredoxin Pet F = reduced ferredoxin Pet F + H+

742279

-

1.12.98.1

H2 + coenzyme F420 = reduced coenzyme F420

659894, 246745, 395080, 395081, 395084, 395086, 395087, 395089, 395097, 395098, 395095, 395082, 395083, 395085, 395092, 395093, 395088, 395090, 395100, 395079, 395094, 687420

-

1.12.98.1

H2 + oxidized coenzyme F420 = reduced coenzyme F420

742368, 743086, 395099, 727399

-

1.12.98.1

H2 + oxidized methyl viologen = reduced methyl viologen

727399

-

1.12.7.2

3 entries

1.12.98.1

3 entries

1.12.98.2

5,10-methenyltetrahydromethanopterin + H2 = 5,10-methylenetetrahydromethanopterin + H+

657579, 658635, 658966, 658978, 696971

-

1.12.98.3

H2 + 2-(2,3-dihydropentaprenyloxy)phenazine = 2-dihydropentaprenyloxyphenazine

440299, 741956, 440300, 727829

-

1.12.99.6

H2 + acceptor = acceptor-H2

658987, 657739

-

1.12.99.6

H2 + acceptor = acceptorH2

658082, 659887

-

1.12.99.6

H2 + acceptor = H+ + reduced acceptor

713374, 712029, 439644, 439637, 439645, 439638, 439627, 439643, 439632, 671432

-

1.12.99.6

H2 + acceptor = reduced acceptor

742652

-

1.12.99.6

H2 + methylene blue = reduced methylene blue

742265

-

1.12.99.6

H2 + NADPH = NADP + H+

743732

-

1.12.99.6

6 entries

1.8.98.1

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 = coenzyme B + coenzyme M

655400, 655398, 655396, 655825

-

1.8.98.5

H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB = reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+

744833, 744179

-

1.97.1.9

selenate + H2 = selenite + H2O

658902

-

In Vivo Product in Enzyme-catalyzed Reactions (17 results)

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Show Natural Product (17 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.12.1.2

H+ + NADH = H2 + NAD+

-

-

1.12.1.2

H+ + NADPH = H2 + NADP+

-

-

1.12.1.2

2 entries

1.12.1.4

NADH + H+ + reduced ferredoxin = H2 + NAD+ + oxidized ferredoxin

-

-

1.12.7.2

2 H+ + reduced ferredoxin = H2 + oxidized ferredoxin

-

-

1.12.7.2

2 H+ + reduced polyferredoxin = H2 + oxidized polyferredoxin

-

-

1.12.7.2

H+ + reduced ferredoxin = H2 + oxidized ferredoxin

-

-

1.18.6.1

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

, 440174, 440188

-

1.18.6.1

N2 + 10 H+ + 8 e- + 16 ATP = 2 NH4+ + H2 + 16 ADP + 16 phosphate

-

-

1.18.6.1

N2 + 8 e- + 16 ATP + 8 H+ = 2 NH3 + H2 + 16 ADP + 16 phosphate

-

-

1.18.6.1

N2 + 8 e- + 8 H+ + 16 ATP = 2 NH3 + H2 + 16 ADP + 16 phosphate

-

-

1.18.6.1

reduced ferredoxin + H+ + N2 + ATP + H2O = oxidized ferredoxin + H2 + NH3 + ADP + phosphate

-

-

1.18.6.1

reduced ferredoxin + H+ + N2 + ATP = oxidized ferredoxin + H2 + NH3 + ADP + phosphate

-

-

1.19.6.1

reduced ferredoxin + H+ + N2 + ATP = oxidized ferredoxin + H2 + NH3 + ADP + phosphate

-

-

1.19.6.1

reduced flavodoxin + N2 + ATP + H2O = oxidized flavodoxin + H2 + NH3 + ADP + phosphate

-

-

1.19.6.1

reduced flavodoxin II + N2 + ATP + H2O = oxidized flavodoxin II + H2 + NH3 + ADP + phosphate

-

-

1.12.7.2

3 entries

1.18.6.1

6 entries

1.19.6.1

3 entries

1.2.7.4

CO + H2O = CO2 + H2

390485

-

1.8.98.5

reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+ = H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB

-

-

Substrate in Enzyme-catalyzed Reactions (176 results)

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Show Substrate (176 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.99.30

2-oxo-3,5-dienoates + H2 = ?

655314

-

1.1.99.30

2-oxo-3-(3,4-dihydroxyphenyl)-propionate + H2 = rosmarinic acid

655314

-

1.1.99.30

2-oxo-3-enoates + H2 = ?

655314

-

1.1.99.30

phenylpyruvate + H2 = (2R)-phenyllactate

655314

-

1.12.1.2

FAD + H2 = FADH2

439702

-

1.12.1.2

H2 + acceptor = H+ + reduced acceptor

672279, 706499

-

1.12.1.2

H2 + ferricyanide = H+ + ferrocyanide

654782, 439711

-

1.12.1.2

H2 + ferrocyanide = H+ + ferricyanide

655890

-

1.12.1.2

H2 + NAD(P)+ = H+ + NAD(P)H

654821

-

1.12.1.2

H2 + NAD+ = H+ + NADH

439633, 439692, 439694, 439695, 439696, 439697, 439700, 439704, 439705, 439706, 439707, 439709, 439710, 439711, 439712, 656298, 672067, 675493, 726344, 654562, 655890, 674165, 654388, 439703, 439713, 742695, 655307, 742373, 742858, 743797, 685324, 656734, 654821, 654285, 655638, 742575, 439699, 439702, 439708, 439701, 439693, 439698, 654782, 672282, 726282

-

1.12.1.2

H2 + NADP+ = H+ + NADPH

672282

-

1.12.1.2

H2 + oxidized benzyl viologen = H+ + reduced benzyl viologen

439633, 439699, 655890, 656298, 654388, 439713, 439702

-

1.12.1.2

H2 + oxidized benzyl viologen = reduced benzyl viologen + H+

655890, 672282

-

1.12.1.2

H2 + oxidized dithionite = H+ + reduced dithionite

654821

-

1.12.1.2

H2 + oxidized methyl viologen = H+ + reduced methyl viologen

439711, 439699, 654388, 439702, 687403, 655307, 656734, 654285, 655638, 744926, 654821

-

1.12.1.2

H2 + oxidized methyl viologen = reduced methyl viologen + H+

672282

-

1.12.1.2

H2 + oxidized methylene blue = H+ + reduced methylene blue

439711, 654388, 439702

-

1.12.1.2

H2 = H+ + e-

654782

-

1.12.1.2

oxidized methylene blue + H2 = reduced methylene blue + H+

439702

-

1.12.1.3

H2 + 2,6-dichlorophenolindophenol = H+ + ?

659019

-

1.12.1.3

H2 + NAD+ = H+ + NADH

659019

-

1.12.1.3

H2 + NADP+ = H+ + NADPH

704606, 439687, 439688, 659019, 439690, 439691, 439686, 687409

-

1.12.1.3

H2 + oxidized benzyl viologen = H+ + reduced benzyl viologen

687409, 659019

-

1.12.1.3

H2 + oxidized ferredoxin = H+ + reduced ferredoxin

439691

-

1.12.1.3

H2 + oxidized methyl viologen = H+ + reduced methyl viologen

687409, 659019, 439687

-

1.12.1.3

H2 + oxidized methylene blue = H+ + reduced methylene blue

659019

-

1.1.99.30

4 entries

1.12.1.2

15 entries

1.12.1.3

7 entries

1.12.1.4

H2 + NAD+ + oxidized ferredoxin = H+ + NADH + reduced ferredoxin

741931

-

1.12.1.5

H2 + NAD+ = H+ + NADH

439641

-

1.12.1.5

H2 + NADP+ = H+ + NADPH

439641

-

1.12.2.1

H2 + acceptor = H+ + reduced acceptor

701780

-

1.12.2.1

H2 + ammonium pertechnetate = ?

686422

-

1.12.2.1

H2 + benzyl viologen = H+ + reduced benzyl viologen

395560

-

1.12.2.1

H2 + ferredoxin = H+ + reduced ferredoxin

395559

-

1.12.2.1

H2 + ferricytochrome c = H+ + ferrocytochrome c

672118, 674919, 395568

-

1.12.2.1

H2 + ferricytochrome c = H+ ferrocytochrome c

395568

-

1.12.2.1

H2 + ferricytochrome c3 = H+ + ferrocytochrome c3

395570, 395559, 395567, 395573, 654261, 657318, 395560, 395561, 395562, 395563, 395566, 395571, 395574, 674919, 684509, 686734, 688655, 742006, 395564, 395565, 689932, 395569, 395572, 742696, 395568, 654899, 675360, 672118, 711266, 724456

-

1.12.2.1

H2 + ferricytochrome c3 = H+ ferrocytochrome c3

395568

-

1.12.2.1

H2 + ferricytochrome Hmc = H+ + ferrocytochrome Hmc

654899

-

1.12.2.1

H2 + methyl viologen = H+ + reduced methyl viologen

395565

-

1.12.2.1

H2 + methylene blue = H+ + reduced methylene blue

395568, 395560, 690013

-

1.12.2.1

H2 + methylviologen-dication = H+ + methylviologen

742696

-

1.12.2.1

H2 + mutant cytochrome c K101M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K15M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K26M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K57M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K58M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K60M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K72M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K94M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c K95M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c Y65A = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c Y66L = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K101M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K10M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K15M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K26M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K57M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K58M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K60M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K72M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K94M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 K95M = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 Y65A = ?

672118

-

1.12.2.1

H2 + mutant cytochrome c3 Y66L = ?

672118

-

1.12.2.1

H2 + oxidized acceptor = H+ + reduced acceptor

704287

-

1.12.2.1

H2 + oxidized dichloroindophenol = H+ + ?

701586

-

1.12.2.1

H2 + oxidized methyl viologen = H+ + reduced methyl viologen

701586

-

1.12.2.1

H2 + potassium ferricyanide = H+ + ?

701586

-

1.12.2.1

H2 + rubredoxin = H+ + reduced rubredoxin

395559

-

1.12.2.1

H2 + [Fe] hydrogenase-cytochrome c3 complex = ?

654899

-

1.12.2.1

H2 + [Fe] hydrogenase-cytochrome Hmc complex = ?

654899

-

1.12.5.1

H2 + 2,3-dimethyl-1,4-naphthoquinone = reduced 2,3-dimethyl-1,4-naphthoquinone

676044, 394192, 394193

-

1.12.5.1

H2 + benzyl viologen = reduced benzyl viologen

394192, 394193, 676044

-

1.12.5.1

H2 + menaquinone = menaquinol

676044

-

1.12.7.2

(SeO3)2- + H2 + ? = Se + H2O + ?

439661

-

1.12.7.2

(TeO3)2- + H2 + ? = Te + H2O + ?

439661

-

1.12.7.2

H2 + 2 oxidized ferredoxin = 2 reduced ferredoxin + 2 H+

730867

-

1.12.7.2

H2 + electron acceptor = H+ + reduced electron acceptor

439668, 439651, 439660, 439643, 439663, 439632, 439669, 439670, 439679, 439681, 439682, 439684, 439671, 439659, 439672, 439665, 439650, 439641, 439652, 439675, 439678, 439674, 439677

-

1.12.7.2

H2 + ferredoxin + oxidized metronidazole = H+ + ferredoxin + reduced metronidazole

673529, 656734

-

1.12.7.2

H2 + methyl viologen = reduced methyl viologen + H+

742859, 742698

-

1.12.7.2

H2 + NAD+ = H+ + NADH

439641

-

1.12.7.2

H2 + oxidized benzyl viologen = H+ + reduced benzyl viologen

439674, 439677

-

1.12.7.2

H2 + oxidized benzyl viologen = reduced benzyl viologen + H+

673529

-

1.12.7.2

H2 + oxidized CAC3527 ferredoxin = H+ + reduced CAC3527 ferredoxin

686267

-

1.12.7.2

H2 + oxidized ferredoxin = H+ + reduced ferredoxin

439643, 685620, 685627, 439665, 439672, 439632, 439669, 439671, 439675, 439679, 439682, 439677, 712281, 712301, 712307, 439654, 688848, 439650, 686267, 684643

-

1.12.7.2

H2 + oxidized ferredoxin = reduced ferredoxin + 2 H+

705334

-

1.12.7.2

H2 + oxidized ferredoxin = reduced ferredoxin + H+

659110, 660222, 742859, 742784, 742698

-

1.12.7.2

H2 + oxidized ferredoxin CAC0587 = H+ + reduced ferredoxin CAC0587

686793

-

1.12.7.2

H2 + oxidized ferredoxin Pet F = reduced ferredoxin Pet F + H+

742279

-

1.12.7.2

H2 + oxidized flavodoxin CAC0587 = H+ + reduced flavodoxin CAC0587

686793

-

1.12.7.2

H2 + oxidized methyl viologen = H+ + reduced methyl viologen

439650, 439669, 439677, 684643, 686267, 686793, 712301, 439654, 688848

-

1.12.7.2

H2 + oxidized methylene blue = H+ + reduced methylene blue

439669

-

1.12.7.2

H2 + oxidized spinach ferredoxin = reduced spinach ferredoxin + H+

742698

-

1.12.98.1

H2 + benzyl viologen = ?

395087, 395100

-

1.12.98.1

H2 + coenzyme F0 = reduced coenzyme F0

395086, 395097, 395089, 395093, 395080

-

1.12.98.1

H2 + coenzyme F420 = reduced coenzyme F420

246745, 659894, 395081, 395084, 395086, 395087, 395089, 395097, 395098, 395095, 395082, 395083, 395085, 395092, 395093, 687420, 395088, 395090, 395100, 395079, 395094, 395080

-

1.12.98.1

H2 + FAD = H+ + FADH2

395093

-

1.12.98.1

H2 + methyl viologen = ?

395080, 395089, 395098, 395095, 395082, 395085, 395093, 395097, 395088

-

1.12.98.1

H2 + oxidized benzyl viologen = reduced benzyl viologen

395099

-

1.12.98.1

H2 + oxidized coenzyme F0 = reduced coenzyme F20

395099

-

1.12.98.1

H2 + oxidized coenzyme F420 = reduced coenzyme F420

742368, 743086, 395099, 727399

-

1.12.98.1

H2 + oxidized methyl viologen = reduced methyl viologen

727399

-

1.12.1.5

2 entries

1.12.2.1

42 entries

1.12.5.1

3 entries

1.12.7.2

19 entries

1.12.98.1

9 entries

1.12.98.2

5,10-methenyltetrahydromethanopterin + H2 = 5,10-methylenetetrahydromethanopterin + H+

657579, 658635, 658966, 658978, 696971

-

1.12.98.3

H2 + 2-(2,3-dihydropentaprenyloxy)phenazine = 2-dihydropentaprenyloxyphenazine

440300, 440299, 741956, 439643, 727829

-

1.12.98.3

H2 + 2-hydroxyphenazine = dihydro-2-hydroxyphenazine

440299, 440300

-

1.12.98.3

H2 + oxidized methyl viologen = reduced methyl viologen

440297

-

1.12.98.4

H2 + benzyl viologen = H2S + reduced benzyl viologen

439641

-

1.12.98.4

H2 + methyl viologen = H2S + reduced methyl viologen

722289, 439641

-

1.12.98.4

H2 + NADP+ = H2S + NADPH + H+

722289

-

1.12.98.4

H2 + polysulfide(n) = H2S + polysulfide(n-1)

439664, 439641

-

1.12.98.4

polysulfide + H2 = H2S

674514

-

1.12.98.4

S + H2 = H2S

674514

-

1.12.98.4

sulfur + H2 = H2S

659878

-

1.12.98.4

tetrathionate + H2 = ? + H+

674514

-

1.12.98.3

3 entries

1.12.98.4

8 entries

1.12.98.B1

H2 + sulfur = H2S

722508

-

1.12.99.6

H2 + 2,3,5-triphenyltetrazolium chloride = reduced 2,3,5-triphenyltetrazolium chloride

742788

-

1.12.99.6

H2 + 2,3-dimethyl-1,4-naphthoquinone = 2,3-dimethyl-naphthoquinol

659355

-

1.12.99.6

H2 + 2,4,6-trinitrotoluene = ?

675221

-

1.12.99.6

H2 + acceptor = acceptor-H2

684608, 658987, 657739, 659041

-

1.12.99.6

H2 + acceptor = acceptorH2

658082, 659887

-

1.12.99.6

H2 + acceptor = H+ + reduced acceptor

439630, 439644, 439633, 674448, 686733, 711862, 439637, 439628, 684463, 713374, 712029, 439638, 671937, 713368, 439645, 439641, 439627, 439643, 439649, 439647, 439631, 439635, 439640, 439648, 439634, 439632, 439636, 439642, 439646, 439629, 671432, 710927

-

1.12.99.6

H2 + acceptor = H2-acceptor

687302, 684596

-

1.12.99.6

H2 + acceptor = reduced acceptor

742652

-

1.12.99.6

H2 + anthraquinone 2,6-disulfonic acid = anthraquinol 2,6-disulfonic acid

657605

-

1.12.99.6

H2 + benzyl viologen = H+ + reduced benzyl viologen

710951

-

1.12.99.6

H2 + benzyl viologen = reduced benzyl viologen

742788

-

1.12.99.6

H2 + Cr6+ = Cr3+ + H+

657622

-

1.12.99.6

H2 + fumarate = succinate

742788

-

1.12.99.6

H2 + methenyltetrahydromethanopterin = H+ + methylenetetrahydromethanopterin

690060, 687827

-

1.12.99.6

H2 + methionaquinone = methionaquinol + H+

439640

-

1.12.99.6

H2 + methyl viologen = H+ + reduced methyl viologen

725206, 711516

-

1.12.99.6

H2 + methylene blue = reduced methylene blue

742265

-

1.12.99.6

H2 + NAD+ = NADH + H+

439641

-

1.12.99.6

H2 + NADPH = NADP + H+

743732

-

1.12.99.6

H2 + O2 = ?

713401, 710927

-

1.12.99.6

H2 + oxidized 2,3-dimethylnaphthoquinone = 2,3-dimethylnaphthoquinol

659878

-

1.12.99.6

H2 + oxidized acceptor = H+ + reduced acceptor

704016, 701778, 704489, 702254, 704162

-

1.12.99.6

H2 + oxidized amaranth = H+ + reduced amaranth

684651

-

1.12.99.6

H2 + oxidized benzyl viologen = H+ + reduced benzyl viologen

439648, 686732, 702218, 702707, 703746

-

1.12.99.6

H2 + oxidized benzyl viologen = H+ + reduceded benzyl viologen

685293

-

1.12.99.6

H2 + oxidized benzyl viologen = reduced benzyl viologen

658082

-

1.12.99.6

H2 + oxidized benzyl viologen = reduced benzyl viologen + H+

658082, 659355, 657605, 659878, 658138, 672638

-

1.12.99.6

H2 + oxidized dichloroindophenol = H+ + reduced dichloroindophenol

711516

-

1.12.99.6

H2 + oxidized diquat = reduced diquat + H+

657605

-

1.12.99.6

H2 + oxidized electron acceptor = H+ + reduced electron acceptor

702307, 704015, 704013, 704012, 687302

-

1.12.99.6

H2 + oxidized ethyl viologen = reduced ethyl viologen + H+

657605

-

1.12.99.6

H2 + oxidized ferredoxin = reduced ferredoxin

439643

-

1.12.99.6

H2 + oxidized methionaquinone = reduced methionaquinone

439640

-

1.12.99.6

H2 + oxidized methyl viologen = H+ + reduced methyl viologen

706442, 684608, 726704, 704309, 688848, 704744, 703746

-

1.12.99.6

H2 + oxidized methyl viologen = reduced methyl viologen

439647

-

1.12.99.6

H2 + oxidized methyl viologen = reduced methyl viologen + H+

657778, 672729, 658972, 657622, 657605, 659878, 659310, 659520, 658138

-

1.12.99.6

H2 + oxidized methylene blue = H+ + reduced methylene blue

711516, 704518, 703746

-

1.12.99.6

H2 + oxidized methylene blue = reduced methylene blue

439646, 703746

-

1.12.99.6

H2 + oxidized methylene blue = reduced methylene blue + H+

674936, 703746

-

1.12.99.6

H2 + oxidized metranidazole = H+ + reduced metranidazol

659523

-

1.12.99.6

H2 + oxidized phenazine methosulfate = reduced phenazine methosulfate + H+

657605

-

1.12.99.6

H2 + oxidized sodium hydrosulfite = H+ + reduced sodium hydrosulfite

711516

-

1.12.99.6

H2 + phenazine methosulfate = H+ + reduced phenazine methosulfate

711516

-

1.12.99.6

H2 + S = H2S

439641

-

1.12.99.6

44 entries

1.14.14.9

2-phenylbutyric acid + H2 = ?

438895

-

1.18.6.2

acetylene + H2 + ? = ethylene + ?

745388

-

1.2.1.B29

H2 + NADP+ + 2 oxidized ferredoxin [iron-sulfur] cluster = NADPH + 3 H+ + 2 reduced ferredoxin [iron-sulfur] cluster

734087

-

1.2.1.B29

H2 + NADP+ + 2 oxidized methyl viologen = ? + NADPH + H+ + 2 reduced methyl viologen

734087

-

1.2.1.B29

2 entries

1.2.7.4

H2 + reduced methyl viologen = methyl viologen

655446

-

1.8.98.1

N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 = coenzyme B + coenzyme M

655396, 655400, 655398, 655471, 655531, 655825, 654385, 639391

-

1.8.98.5

H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB = reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+

744833, 744179

-

1.8.98.5

H2 + oxidized methyl viologen + CoM-S-S-CoB = reduced methyl viologen + CoB + CoM + H+

744833, 744179

-

1.8.98.5

2 entries

1.97.1.9

selenate + H2 = selenite + H2O

658902

-

3.1.2.1

H2 + CO2 + CH3-H4SPt + CoASH = acetyl-CoA + H4SPt + H2O

94491

-

3.1.3.7

3'-phosphoadenosine 5'-phosphate + H2 = AMP + phosphate

730884

-

3.4.21.B12

urokinase-type plasminogen activator receptor + H2 = ?

664270

-

3.4.22.15

fibronectin + H2 = ?

727746

-

3.4.22.15

laminin + H2 = ?

727746

-

3.4.22.15

vitronectin + H2 = ?

727746

-

3.4.22.15

3 entries

3.4.22.B6

fibronectin + H2 = ?

754980

-

7.1.1.9

reduced N,N,N',N'-tetramethyl-4-phenylenediamine + O2 + H2 = oxidized N,N,N',N'-tetramethyl-4-phenylenediamine + H2O

743382

-

Product in Enzyme-catalyzed Reactions (52 results)

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Show Product (52 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.11.1.16

2 Mn2+ + 2 H+ = 2 Mn3+ + H2

-

-

1.12.1.2

H+ + NADH + reduced ferredoxin = H2 + NAD+ + oxidized ferredoxin

-

-

1.12.1.2

H+ + NADH = H2 + NAD+

-

-

1.12.1.2

H+ + NADPH = H2 + NADP+

-

-

1.12.1.2

H+ + reduced dithionite = H2 + oxidized dithionite

-

-

1.12.1.2

H+ + reduced methyl viologen = H2 + methyl viologen

-

-

1.12.1.2

H+ + reduced methyl viologen = H2 + oxidized methyl viologen

-

-

1.12.1.3

H+ + NADPH = H2 + NADP+

-

-

1.12.1.3

H+ + reduced benzyl viologen = H2 + oxidized benzyl viologen

-

-

1.12.1.3

NADPH + H+ = H2 + NADP+

-

-

1.12.1.4

NADH + H+ + reduced ferredoxin = H2 + NAD+ + oxidized ferredoxin

-

-

1.12.1.4

NADH + H+ + reduced methyl viologen = H2 + NAD+ + oxidized methyl viologen

-

-

1.12.1.4

sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid = H2 + oxidized anthraquinone-2,6-disulfonic acid + ?

-

-

1.12.1.4

sodium dithionite + H+ + reduced methyl viologen = H2 + oxidized methyl viologen + ?

-

-

1.12.1.5

H+ + NADPH = H2 + NADP+

-

-

1.12.1.5

H+ + reduced methyl viologen = H2 + oxidized methyl viologen

-

-

1.12.2.1

H+ + ferrocytochrome c3 = H2 + ferricytochrome c3

-

-

1.12.2.1

H+ + neutral red = H2 + oxidized neutral red

-

-

1.12.2.1

H+ + phenosafranine = H2 + oxidized phenosafranine

-

-

1.12.2.1

H+ + reduced methyl viologen = H2 + methyl viologen

-

-

1.12.2.1

reduced methyl viologen + H+ = oxidized methyl viologen + H2

-

-

1.12.7.2

2 H+ + reduced ferredoxin = H2 + oxidized ferredoxin

-

-

1.12.7.2

2 H+ + reduced polyferredoxin = H2 + oxidized polyferredoxin

-

-

1.12.7.2

H+ + NADH = H2 + NAD+

-

-

1.12.7.2

H+ + reduced benzyl viologen = H2 + oxidized benzyl viologen

-

-

1.12.7.2

H+ + reduced ferredoxin = H2 + oxidized ferredoxin

-

-

1.12.7.2

H+ + reduced methyl viologen = H2 + oxidized methyl viologen

-

-

1.12.98.2

H+ + 5,10-methylenetetrahydromethanopterin = H2 + 5,10-methenyltetrahydromethanopterin

-

-

1.12.98.2

N5,N10-methylenetetrahydromethanopterin + H+ = N5,N10-methenyltetrahydromethanopterin + H2

, 394696

-

1.12.98.4

H2S + NADH + H+ = H2 + NAD+

-

-

1.12.98.4

H2S + NADPH + H+ = H2 + NADP+

-

-

1.12.99.6

formate = H2 + CO2

-

-

1.12.99.6

reduced ferredoxin = H2 + oxidized ferredoxin

-

-

1.12.1.2

6 entries

1.12.1.3

3 entries

1.12.1.4

4 entries

1.12.1.5

2 entries

1.12.2.1

5 entries

1.12.7.2

6 entries

1.12.98.2

2 entries

1.12.98.4

2 entries

1.12.99.6

2 entries

1.14.14.32

17alpha-hydroxypregnenolone + [reduced NADPH-hemoprotein reductase] + O2 = 3beta-hydroxyandrost-5-en-17-one + acetate + [oxidized NADPH-hemoprotein reductase] + H2

-

-

1.18.6.1

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

, 440145, 440134, 440143, 440144, 440151, 440142, 440171, 440176, 440177, 440140, 440146, 440147, 440149, 440152, 440157, 440166, 440174, 440153, 440159, 440164, 440180, 440150, 440155, 440156, 440158, 440163, 440178, 440179, 440181, 440183, 440184, 440187, 440188, 440189, 440169, 440162, 440148, 440165, 440170, 440161, 440167, 440168, 440160, 440138, 440154, 440186, 440175, 440182

-

1.18.6.1

C2H2 + ? = H2 + ?

-

-

1.18.6.1

N2 + 10 H+ + 8 e- + 16 ATP = 2 NH4+ + H2 + 16 ADP + 16 phosphate

-

-

1.18.6.1

N2 + 8 e- + 16 ATP + 8 H+ = 2 NH3 + H2 + 16 ADP + 16 phosphate

-

-

1.18.6.1

N2 + 8 e- + 8 H+ + 16 ATP = 2 NH3 + H2 + 16 ADP + 16 phosphate

-

-

1.18.6.1

reduced ferredoxin + H+ + ATP = oxidized ferredoxin + H2 + ADP + phosphate

440184, 440187, 440189, , 440176

-

1.18.6.1

reduced ferredoxin + H+ + N2 + ATP + H2O = oxidized ferredoxin + H2 + NH3 + ADP + phosphate

-

-

1.18.6.1

reduced ferredoxin + H+ + N2 + ATP = oxidized ferredoxin + H2 + NH3 + ADP + phosphate

-

-

1.18.6.1

8 entries

1.18.6.2

CO + H+ + ATP = C2H4 + C2H6 + C3H8 + H2O + H2 + phosphate

-

-

1.19.6.1

reduced ferredoxin + H+ + N2 + ATP = oxidized ferredoxin + H2 + NH3 + ADP + phosphate

-

-

1.19.6.1

reduced flavodoxin + N2 + ATP + H2O = oxidized flavodoxin + H2 + NH3 + ADP + phosphate

-

-

1.19.6.1

reduced flavodoxin II + N2 + ATP + H2O = oxidized flavodoxin II + H2 + NH3 + ADP + phosphate

-

-

1.19.6.1

3 entries

1.2.1.B29

NADPH + 3 H+ + 2 reduced ferredoxin [iron-sulfur] cluster = H2 + NADP+ + 2 oxidized ferredoxin [iron-sulfur] cluster

-

-

1.2.7.4

CO + H2O + methylene blue = CO2 + reduced methylene blue + H2

-

-

1.2.7.4

CO + H2O = CO2 + H2

390485

-

1.2.7.4

2 entries

1.8.98.1

coenzyme B + coenzyme M + reduced ferredoxin + H+ + coenzyme F420 = coenzyme B-coenzyme M disulfide + ferredoxin + H2 + reduced coenzyme F420

-

-

1.8.98.5

reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+ = H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB

-

-

1.8.98.5

reduced metronidazole + CoB + CoM + H+ = H2 + oxidized metronidazole + CoM-S-S-CoB

-

-

1.8.98.5

2 entries

Activator in Enzyme-catalyzed Reactions (2 results)

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Show Activating Compound (2 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

2.8.4.1

suggested to be involved in the activation of the methylreductase, preincubation

393257

-

7.2.2.11

stimulates

210289

-

Inhibitor in Enzyme-catalyzed Reactions (10 results)

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Show Inhibitors (10 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.176

H2 strongly inhibits bile acid oxidation by the enzyme

761221

-

1.12.98.2

inhibits formation of N5,N10-methenyltetrahydromethanopterin

394694

-

1.12.99.6

inhibits hydrogen production

674190

-

1.18.6.1

-

440144, 440146, 440154, 440158

-

1.18.6.1

competitive

440183

-

1.18.6.1

competitive inhibition of N2 binding

658040

-

1.18.6.1

competitive inhibitor of N2, no inhibition of N3-, C2H2, CN- or H+ reduction

440146

-

1.18.6.1

H2 competes with N2 binding and inhibits N2 reduction by the FeMo protein, but H2 does not inhibit NO2- reduction for the wild-type or either of the two MoFe protein variants

742740

-

1.18.6.1

5 entries

1.18.6.2

CO-reduction by V-nitrogenase is inhibited by the addition of increasing amounts of H2

746492

-

1.19.6.1

inhibits both N2 and diazene reduction

685165

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (11 results)

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Show Turnover Number (11 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.12.1.2

70

-

pH 8.0, HoxI deletion mutant enzyme, with oxidized benzyl viologen

655890

1.12.1.2

109

-

pH 8.0, HoxI deletion mutant enzyme, with NAD+

655890

1.12.1.2

120

-

pH 8.0, wild-type enzyme complex including HoxI, with oxidized benzyl viologen

655890

1.12.1.2

143

-

pH 8.0, wild-type enzyme complex including HoxI, with NAD+

655890

1.12.1.2

212

-

-

439706

1.12.1.2

284

-

pH 8.0, HoxI deletion mutant enzyme, with ferrocyanide

655890

1.12.1.2

372

-

pH 8.0, wild-type enzyme complex including HoxI, with ferrocyanide

655890

1.12.7.2

0.683

-

-

439659

1.12.7.2

133

-

using ferredoxin CAC0587 as electron carrier, at 35°C

686793

1.12.7.2

454

-

using methyl viologen as electron carrier, at 35°C

686793

1.12.7.2

483

-

using flavodoxin CAC0587 as electron carrier, at 35°C

686793

1.12.1.2

7 entries

1.12.7.2

4 entries

K_M Value (39 results)

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Show KM Value (39 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.12.1.2

0.037

-

-

439699

1.12.1.2

0.063

-

-

439701

1.12.1.2

0.19

-

-

439693

1.12.1.3

0.012

-

-

439691

1.12.1.3

0.018

-

cosubstrate: benzyl viologen

687409

1.12.1.3

0.02

-

cosubstrate: methyl viologen

687409

1.12.1.2

3 entries

1.12.1.3

3 entries

1.12.1.4

-999

-

apparent Km value for H2 is about 6% in the gas phase, pH 7.5, 45°C

740618

1.12.7.2

0.000033

-

using ferredoxin CAC0587 as electron carrier, at 35°C

686793

1.12.7.2

0.000088

-

using flavodoxin CAC0587 as electron carrier, at 35°C

686793

1.12.7.2

0.005

-

-

439643, 659110

1.12.7.2

0.016

-

using methyl viologen as electron carrier, at 35°C

686793

1.12.7.2

0.341

-

-

439659

1.12.7.2

5.6

-

-

439663

1.12.98.1

0.002

-

+ methyl viologen, pH 9

395080

1.12.98.1

0.003

-

37°C, pH 7.2, cosubstrate: oxidized coenzyme F420

395099

1.12.98.1

0.004

-

37°C, pH 7.2, cosubstrate: oxidized methyl viologen

395099

1.12.98.1

0.01

-

+ coenzyme F0, pH 7.2

395080

1.12.98.1

0.012

-

-

395089

1.12.98.2

-999

-

apparent Km for H2 is 60%

727402

1.12.98.2

-999

-

the apparent Km for H2 is estimated to be approximately 60% H2 in the gas phase

727402

1.12.98.2

0.15

-

-

394696

1.12.99.6

0.000047

-

apparent value, pH and temperature not specified in the publication

711862

1.12.99.6

0.0004

-

at pH 5.5, 10°C

713401

1.12.99.6

0.001

-

-

439646

1.12.99.6

0.001

-

at pH 5.5, 20°C

713401

1.12.99.6

0.002

-

-

439648

1.12.99.6

0.005

-

-

439643

1.12.99.6

0.006

-

at pH 4.5, 30°C

713401

1.12.99.6

0.006

-

at pH 5.5, 30°C

713401

1.12.99.6

0.008

-

at pH 5.0, 30°C

713401

1.12.99.6

0.01

-

at pH 6.5, 30°C

713401

1.12.99.6

0.0113

-

-

439647

1.12.99.6

0.015

-

at pH 6.0, 30°C

713401

1.12.99.6

0.024

-

at pH 5.5, 40°C

713401

1.12.99.6

0.08

-

with methylene blue as electron acceptor

439632

1.12.99.6

0.41

-

with methyl viologen as electron acceptor

439634

1.12.99.6

1.9

-

with methyl viologen as electron acceptor

439632

1.12.99.6

25

-

-

439631

1.12.7.2

6 entries

1.12.98.1

5 entries

1.12.98.2

3 entries

1.12.99.6

17 entries

1.8.98.1

0.03

-

pH 7, 65°C, H2: heterodisulfide oxidoreductase complex

655398

References & Links

Literature References (342)

top print hide

Show Reference (342 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

94491

7718564

Substrate and accessory protein requirements and thermodynamics of acetyl-CoA synthesis and cleavage in Methanosarcina barkeri

1995

Grahame, D.A.; de Moll, E.

Biochemistry

34

4617-4624

210289

2757374

Nickel transport by the thermophilic acetogen Acetogenium kivui

1989

Yang, H.; Daniel, S.L.; Hsu, T.; Drake, H.L.

Appl. Environ. Microbiol.

55

1078-1081

246745

-

Effect of methanogenic substrates on coenzyme f420-dependent N5,N10-methylene-H4MPT dehydrogenase, N5,N10-methenyl-H4MPT cyclohydrolase and F420-reducing hydrogenase activities in Methanosarcina barkeri

1993

Mukhopadhyay, B.; Purwantini, E.; Daniels, L.

Arch. Microbiol.

159

141-146

390485

11509720

Crystal structure of a carbon monoxide dehydrogenase reveals a [Ni-4Fe-5S] cluster

2001

Dobbek, H.; Svetlitchnyi, V.; Gremer, L.; Huber, R.; Meyer, O.

Science

293

1281-1285

393257

3137210

Reductive activation of the methyl coenzyme M methylreductase system of Methanobacterium thermoautotrophicum.DELTA.H

1988

Rouviere, P.E.; Bobik, T.A.; Wolfe, R.S.

J. Bacteriol.

170

3946-3952

394192

1587288

The quinone-reactive Ni/Fe-hydrogenase of Wolinella succinogenes

1992

Dross, F.; Geisler, V.; Lenger, R.; Theis, F.; Krafft, T.; Fahrenholz, F.; Kojro, E.; Duchene, A.; Tripier, D.; Juvenal, K.; Krueger, A.

Eur. J. Biochem.

206

93-102

394193

8319698

Erratum

1993

Dross, F.; Geisler, V.; Lenger, R.; Theis, F.; Krafft, T.; Fahrenholz, F.; Kojro, E.; Duchene, A.; Tripier, D.; Juvenal, K.; Krueger, A.

Eur. J. Biochem.

214

949-950

394694

-

N5,N10-Methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum has hydrogenase activity

1990

Zirngibl, C.; Hedderich, R.; Thauer, R.K.

FEBS Lett.

261

112-116

394696

8599536

Purification, properties and primary structure of H2-forming N5,N10-methylenetetrahydromethanopterin dehydrogenase from Methanococcus thermolithotrophicus

1996

Hartmann, G.C.; Klein, A.R.; Linder, M.; Thauer, R.K.

Arch. Microbiol.

165

187-193

395079

6211447

A selenium-containing hydrogenase from Methanococcus vannielii. Identification of the selenium moiety as a selenocysteine residue

1982

Yamazaki, S.

J. Biol. Chem.

257

7926-7929

395080

7061485

Purification and properties of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum

1982

Jacobson, F.S.; Daniels, L.; Fox, J.A.; Walsh, C.T.; Orme-Johnson, W.H.

J. Biol. Chem.

257

3385-3388

395081

6300837

Paramagnetic centers in the nickel-containig, deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum

1983

Kojima, N.; Fox, J.A.; Hausinger, R.P.; Daniels, L.; Orme-Johnson, W.H.; Walsh, C.

Proc. Natl. Acad. Sci. USA

80

378-382

395082

-

Two hydrogenases with distinct electron-carrier specificity and subunit composition in Methanobacterium formicicum

1983

Jin, S.L.C.; Blanchard, D.K.; Chen, J.S.

Biochim. Biophys. Acta

748

8-20

395083

6375661

FAD requirement for the reduction of coenzyme F420 by hydrogenase from Methanobacterium formicicum

1984

Nelson, M.J.K.; Brown, D.P.; Ferry, J.G.

Biochem. Biophys. Res. Commun.

120

775-781

395084

3804976

Electron microscopy of nickel-containing methanogenic enzymes: methyl reductase and F420-reducing hydrogenase

1987

Wackett, L.P.; Hartwieg, E.A.; King, J.A.; Orme-Johnson, W.H.; Walsh, C.T.

J. Bacteriol.

169

718-727

395085

3301819

Locations of the hydrogenases of Methanobacterium formicicum after subcellular fractionation of cell extract

1987

Baron, S.F.; Brown, D.P.; Ferry, J.G.

J. Bacteriol.

169

3823-3825

395086

3663585

8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization

1987

Fox, J.A.; Livingston, D.J.; Orme-Johnson, W.H.; Walsh, C.T.

Biochemistry

26

4219-4227

395087

-

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