Inhibitors | Comment | Organism | Structure |
---|---|---|---|
mercaptoethanol | - |
Hydrogenomonas sp. | |
Sulfide | - |
Hydrogenomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
NAD+ | - |
Hydrogenomonas sp. | |
0.19 | - |
H2 | - |
Hydrogenomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Hydrogenomonas sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | Fe2+ stabilizes | Hydrogenomonas sp. | |
Mg2+ | stabilizes | Hydrogenomonas sp. | |
Mn2+ | stabilizes | Hydrogenomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenomonas sp. | - |
- |
- |
Hydrogenomonas sp. H16 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hydrogenomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.5 | - |
- |
Hydrogenomonas sp. |
Storage Stability | Organism |
---|---|
-16°C, 10 d, 30% loss of activity | Hydrogenomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + NAD+ | electron acceptor FAD, only in presence of catalytic amounts of NAD+ | Hydrogenomonas sp. | H+ + NADH | - |
? | |
H2 + NAD+ | artificial electron acceptor methylene blue, only in presence of catalytic amounts of NAD+ | Hydrogenomonas sp. | H+ + NADH | - |
? | |
H2 + NAD+ | electron acceptor FMN, only in presence of catalytic amounts of NAD+ | Hydrogenomonas sp. | H+ + NADH | - |
? | |
H2 + NAD+ | electron acceptor FAD, only in presence of catalytic amounts of NAD+ | Hydrogenomonas sp. H16 | H+ + NADH | - |
? | |
H2 + NAD+ | artificial electron acceptor methylene blue, only in presence of catalytic amounts of NAD+ | Hydrogenomonas sp. H16 | H+ + NADH | - |
? | |
H2 + NAD+ | electron acceptor FMN, only in presence of catalytic amounts of NAD+ | Hydrogenomonas sp. H16 | H+ + NADH | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | - |
- |
Hydrogenomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
potassium phosphate buffer | Hydrogenomonas sp. |
8.5 | - |
Tris-HCl buffer | Hydrogenomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.9 | 9.1 | Tris-HCl buffer and potassium phosphate buffer pH 6.9: 20% activity, Tris-HCl buffer pH 9.1: 40% activity, potassium phosphate buffer pH 9.1: 65% activity | Hydrogenomonas sp. |