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1.6
2,6-dichlorophenolindophenol
-
pH 8.8, 25°C
9.5
2-amino-D-glucose
-
wild-type enzyme
0.000004 - 90
2-deoxy-D-glucose
10.8
3-deoxy-D-glucose
-
wild-type enzyme
2 - 198
3-O-methyl-D-glucose
0.73 - 2.43
4-(4-ferrocenylimino-methyl)phenol
0.89 - 2.73
4-ferrocenylnitrophenol
0.83 - 1
4-ferrocenylphenol
1.3
6-deoxy-D-glucose
-
wild-type enzyme
0.00019 - 0.00065
alpha-D-lactose
0.00007 - 0.0003
D-cellobiose
390
D-fructose
in 50 mM Bis-Tris propane (pH 8.0), at 50°C
0.00011 - 390
D-galactose
0.00008
D-glucose 6-phosphate
pH 7.5, 70°C, wild-type enzyme
17.7
D-melibiose
-
wild-type enzyme
0.00018
ethanol
pH 7.5, 70°C, wild-type enzyme
0.69
ferricyanide
-
pH 8.8, 25°C
100
L-Lyxose
-
wild-type enzyme
0.00013 - 150
maltotriose
0.00009
mannitol
pH 7.5, 70°C, wild-type enzyme
0.00015
methanol
pH 7.5, 70°C, wild-type enzyme
0.56
N,N,N,N-tetramethyl-o-phenylenediamine
-
pH 8.8, 25°C
0.268 - 0.292
N-ethylphenazonium ethyl sulfate
0.064 - 0.4
N-methylphenazonium methyl sulfate
0.074 - 1.9
phenazine methosulfate
0.00011
pyrroliquinoline quinone
-
pH 7.0, 25°C, wild-type enzyme
0.00005 - 0.78
pyrroloquinoline quinone
0.83
sucrose
pH 5.5, 30°C
0.0001 - 0.00113
ubiquinone
0.06 - 0.178
ubiquinone Q1
0.012 - 0.061
ubiquinone Q2
0.028 - 0.031
ubiquinone Q4
0.0034 - 0.019
ubiquinone Q6
0.0044
ubiquinone Q9
-
membrane-bound enzyme form
0.00009
xylitol
pH 7.5, 70°C, wild-type enzyme
additional information
additional information
-
0.000004
2-deoxy-D-glucose
pH 7.5, 70°C, wild-type enzyme
0.00008
2-deoxy-D-glucose
pH 7.5, 70°C, mutant enzyme Y156A
0.00014
2-deoxy-D-glucose
pH 7.5, 70°C, mutant enzyme Y156K
0.5
2-deoxy-D-glucose
-
at pH 7.0 and 25°C
1.6
2-deoxy-D-glucose
-
wild-type enzyme
3.5
2-deoxy-D-glucose
recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C
13.6
2-deoxy-D-glucose
-
-
22
2-deoxy-D-glucose
-
pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor
32
2-deoxy-D-glucose
-
mutant enzyme H262
88
2-deoxy-D-glucose
-
pH 7.0, mutant enzyme E277K
90
2-deoxy-D-glucose
-
pH 7.0, wild-type enzyme
2 - 3
3-O-methyl-D-glucose
-
recombinant Arg-tagged enzyme, pH 7.0
22
3-O-methyl-D-glucose
-
recombinant chimeric mutant enzyme, pH 7.0
27
3-O-methyl-D-glucose
-
pH 7.0, mutant enzyme E277K
27.6
3-O-methyl-D-glucose
-
N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C
27.6
3-O-methyl-D-glucose
recombinant mutant N452T, pH 7.0
28.7
3-O-methyl-D-glucose
recombinant wild-type isozyme PQQGDH-B, pH 7.0
28.7
3-O-methyl-D-glucose
-
wild-type isozyme PQQGDH-B, pH 7.0, 25°C
28.8
3-O-methyl-D-glucose
-
N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C
41
3-O-methyl-D-glucose
recombinant wild-type enzyme, pH 7.0, 25°C
46
3-O-methyl-D-glucose
-
pH 7.0, wild-type enzyme
53
3-O-methyl-D-glucose
recombinant tagged enzyme, pH 7.0, 25°C
56
3-O-methyl-D-glucose
recombinant triple mutant enzyme, pH 7.0, 25°C
79
3-O-methyl-D-glucose
-
wild-type enzyme
79
3-O-methyl-D-glucose
recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C
99
3-O-methyl-D-glucose
recombinant mutant D167E, pH 7.0
198
3-O-methyl-D-glucose
recombinant mutant D167E/N452T, pH 7.0
0.73
4-(4-ferrocenylimino-methyl)phenol
-
s-GDH
2.43
4-(4-ferrocenylimino-methyl)phenol
-
m-GDH
0.89
4-ferrocenylnitrophenol
-
s-GDH
2.73
4-ferrocenylnitrophenol
-
m-GDH
0.83
4-ferrocenylphenol
-
m-GDH
1
4-ferrocenylphenol
-
s-GDH
21
allose
-
pH 7.0, mutant enzyme E277K
32.5
allose
-
N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C
34
allose
-
recombinant Arg-tagged enzyme, pH 7.0
35.5
allose
recombinant wild-type isozyme PQQGDH-B, pH 7.0
35.5
allose
-
wild-type isozyme PQQGDH-B, pH 7.0, 25°C
36
allose
-
recombinant chimeric mutant enzyme, pH 7.0
38.7
allose
-
N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C
38.7
allose
recombinant mutant N452T, pH 7.0
63
allose
recombinant wild-type enzyme, pH 7.0, 25°C
75
allose
recombinant tagged enzyme, pH 7.0, 25°C
76
allose
recombinant triple mutant enzyme, pH 7.0, 25°C
182
allose
recombinant mutant D167E/N452T, pH 7.0
199
allose
recombinant mutant D167E, pH 7.0
0.00019
alpha-D-lactose
pH 7.5, 70°C, wild-type enzyme
0.00064
alpha-D-lactose
pH 7.5, 70°C, mutant enzyme Y156A
0.00065
alpha-D-lactose
pH 7.5, 70°C, mutant enzyme Y156K
0.54
cellobiose
pH 5.5, 30°C
14
cellobiose
recombinant wild-type isozyme PQQGDH-B and mutant N452T, pH 7.0
16
cellobiose
recombinant mutant D167E/N452T, pH 7.0
17
cellobiose
recombinant mutant D167E, pH 7.0
1.5
D-allose
-
-
2.5
D-allose
-
wild-type enzyme
29
D-allose
-
pH 7.0, wild-type enzyme
810
D-allose
-
mutant enzyme H262Y
0.00007
D-cellobiose
pH 7.5, 70°C, wild-type enzyme
0.00023
D-cellobiose
pH 7.5, 70°C, mutant enzyme Y156K
0.0003
D-cellobiose
pH 7.5, 70°C, mutant enzyme Y156A
5
D-fucose
-
pH 8.5, reaction with N-ethylphenazonium ethyl sulfate as electron acceptor
8.3
D-fucose
-
wild-type enzyme
12
D-fucose
-
pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor
0.00011
D-galactose
pH 7.5, 70°C, wild-type enzyme
0.0002
D-galactose
pH 7.5, 70°C, mutant enzyme Y156K
0.00038
D-galactose
pH 7.5, 70°C, mutant enzyme Y156A
0.46
D-galactose
-
at pH 5.0 and 45°C
0.6
D-galactose
pH 5.5, 30°C
2
D-galactose
recombinant wild-type enzyme, pH 7.0, 25°C
2.7
D-galactose
-
N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C
3.7
D-galactose
-
N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C
3.7
D-galactose
recombinant mutant N452T, pH 7.0
4
D-galactose
recombinant tagged enzyme, pH 7.0, 25°C
5
D-galactose
recombinant triple mutant enzyme, pH 7.0, 25°C
5.3
D-galactose
recombinant wild-type isozyme PQQGDH-B, pH 7.0
5.3
D-galactose
-
wild-type isozyme PQQGDH-B, pH 7.0, 25°C
6.8
D-galactose
-
pH 7.0, wild-type enzyme and mutant enzyme E277K
8
D-galactose
-
recombinant Arg-tagged enzyme, pH 7.0
9
D-galactose
-
recombinant chimeric mutant enzyme, pH 7.0
17.5
D-galactose
recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C
19
D-galactose
-
pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor
39
D-galactose
-
wild-type enzyme
40
D-galactose
-
at pH 7.0 and 25°C
145
D-galactose
recombinant mutant D167E/N452T, pH 7.0
390
D-galactose
in 50 mM Bis-Tris propane (pH 8.0), at 50°C
0.00002
D-glucosamine
pH 7.5, 70°C, wild-type enzyme
0.00002
D-glucosamine
pH 7.5, 70°C, mutant enzyme Y156A
0.00002
D-glucosamine
pH 7.5, 70°C, mutant enzyme Y156K
0.00021
D-glucose
pH 7.5, 70°C, wild-type enzyme
0.00025
D-glucose
pH 7.5, 70°C, mutant enzyme Y156K
0.00047
D-glucose
pH 7.5, 70°C, mutant enzyme Y156A
0.1
D-glucose
-
at pH 7.0 and 25°C
0.21
D-glucose
-
at pH 5.0 and 45°C
0.23
D-glucose
-
pH 8.5, reaction with 2,6-dichlorophenolindophenol as electron acceptor
0.3
D-glucose
-
pH 7.0, mutant enzyme E277G
0.41
D-glucose
pH 5.5, 30°C
0.47
D-glucose
-
pH 6.0, 25°C, reaction with 2,6-dichlorophenolindophenol
0.8
D-glucose
-
pH 7.0, 25°C, mutant enzyme N607A
0.8
D-glucose
-
25°C, mutant enzyme D730 N
0.9
D-glucose
-
pH 7.0, 25°C, wild-type enzyme
0.91
D-glucose
-
25°C, wild-type enzyme
0.95
D-glucose
-
wild-type enzyme
0.98
D-glucose
-
C-terminal periplasmic domain of glucose dehydrogenase
1
D-glucose
-
25°C, mutant enzyme S357L
1.2
D-glucose
-
pH 7.0, 25°C, mutant W404A
1.2
D-glucose
-
mutant E277N
1.3
D-glucose
-
25°C, mutant enzyme H775R
1.4
D-glucose
-
25°C, mutant enzyme G689D
1.4
D-glucose
-
pH 7.0, 25°C, mutant K493A
1.5
D-glucose
-
pH 7.0, 25°C, mutant K493R
1.5
D-glucose
-
mutant enzyme E277A
2
D-glucose
-
pH 7.0, 21°C, mutant W404F
2 - 3
D-glucose
-
recombinant Arg-tagged enzyme, pH 7.0
2 - 3
D-glucose
-
recombinant cytochrome c-fusion protein, pH 7.0
2.1
D-glucose
-
wild-type enzyme
2.4
D-glucose
-
wild type enzyme, in 42 mM sodium phosphate buffer pH 7.5 at 37°C
2.5
D-glucose
-
pH 7.0, mutant enzyme E277V
2.71
D-glucose
-
recombinant enzyme, at pH 7.5 and 28°C
2.8
D-glucose
recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C
3
D-glucose
-
pH 7.0, 25°C, mutant D466E
4
D-glucose
-
pH 8.5, reaction with N-methylphenazonium methyl sulfate or N-ethylphenazonium ethal sulfate as electron acceptor
4.2
D-glucose
-
membrane-bound enzyme form
4.3
D-glucose
-
pH 7.0, mutant enzyme E277Q
4.91
D-glucose
-
native enzyme, at pH 7.5 and 28°C
5.9
D-glucose
-
pH 8.5, reaction with Wurster Blue as electron acceptor
5.9
D-glucose
-
recombinant Strep-tagged enzyme, pH 6.0, 25°C
7
D-glucose
-
pH 7.0, mutant enzyme I278F
7.2
D-glucose
-
mutant enzyme Q219K/F220C, in 42 mM sodium phosphate buffer pH 7.5 at 37°C
7.4
D-glucose
-
pH 7.0, mutant enzyme E277D
7.6
D-glucose
-
mutant enzyme Q219K/F220K, in 42 mM sodium phosphate buffer pH 7.5 at 37°C
7.7
D-glucose
-
pH 7.0, mutant enzyme E277H
8.8
D-glucose
-
pH 7.0, mutant enzyme E277K
8.9
D-glucose
-
pH 7.0, mutant enzyme E277K
10
D-glucose
-
pH 7.0, 25°C, mutant H262A
10.6
D-glucose
-
mutant enzyme Q219N/F220K, in 42 mM sodium phosphate buffer pH 7.5 at 37°C
11
D-glucose
-
mutant enzyme Q126E, in 42 mM sodium phosphate buffer pH 7.5 at 37°C
11.3
D-glucose
-
mutant enzyme Q219E/F220E, in 42 mM sodium phosphate buffer pH 7.5 at 37°C
12
D-glucose
-
pH 7.0, 25°C, mutant D466N
12.3
D-glucose
-
N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C
12.5
D-glucose
-
N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C
12.5
D-glucose
recombinant mutant N452T, pH 7.0
15.7
D-glucose
-
pH 7.0, mutant enzyme N279H
16
D-glucose
recombinant mutant T416V/T417V, pH 7.0, 25°C
20
D-glucose
-
pH 7.0, 25°C, linked dimeric enzyme
20
D-glucose
recombinant wild-type enzyme and mutants N340F/Y418F and N340F/Y418I, pH 7.0, 25°C
22
D-glucose
-
recombinant chimeric mutant enzyme, pH 7.0
22.6
D-glucose
-
mutant enzyme Q126S, in 42 mM sodium phosphate buffer pH 7.5 at 37°C
24
D-glucose
-
pH 7.0, mutant enzyme D275E and D276E
24.5
D-glucose
-
soluble enzyme
25
D-glucose
recombinant triple mutant enzyme, pH 7.0, 25°C
25
D-glucose
recombinant wild-type isozyme PQQGDH-B, pH 7.0
25
D-glucose
-
wild-type isozyme PQQGDH-B, pH 7.0, 25°C
26
D-glucose
-
pH 7.0, wild-type enzyme
27
D-glucose
recombinant wild-type enzyme and tagged enzyme, pH 7.0, 25°C
41.4
D-glucose
-
mutant enzyme Q126R, in 42 mM sodium phosphate buffer pH 7.5 at 37°C
48
D-glucose
recombinant mutant D167E/N452T, pH 7.0
55
D-glucose
recombinant mutant D167E, pH 7.0
154
D-glucose
recombinant mutant H168Q, pH 7.0
193
D-glucose
recombinant mutant H168C, pH 7.0
460
D-glucose
-
mutant enzyme H262Y
680
D-glucose
in 50 mM Bis-Tris propane (pH 8.0), at 50°C
0.00013
D-mannose
pH 7.5, 70°C, wild-type enzyme
0.00014
D-mannose
pH 7.5, 70°C, mutant enzyme Y156A
0.00016
D-mannose
pH 7.5, 70°C, mutant enzyme Y156K
22
D-mannose
-
pH 7.0, wild-type enzyme and mutant enzyme E277K
78
D-mannose
-
wild-type enzyme
116
D-mannose
recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C
910
D-mannose
in 50 mM Bis-Tris propane (pH 8.0), at 50°C
0.00007
D-ribose
pH 7.5, 70°C, mutant enzyme Y156A
0.00013
D-ribose
pH 7.5, 70°C, wild-type enzyme
0.00044
D-ribose
pH 7.5, 70°C, mutant enzyme Y156K
110
D-ribose
-
wild-type enzyme
166
D-ribose
recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C
0.00014
D-xylose
pH 7.5, 70°C, wild-type enzyme
0.00023
D-xylose
pH 7.5, 70°C, mutant enzyme Y156K
0.00046
D-xylose
pH 7.5, 70°C, mutant enzyme Y156A
0.34
D-xylose
-
at pH 5.0 and 45°C
0.67
D-xylose
pH 5.5, 30°C
7
D-xylose
-
pH 8.5, reaction with N-ethylphenazonium ethyl sulfate as electron acceptor
12
D-xylose
-
pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor
14.3
D-xylose
-
pH 7.0, wild-type enzyme
17
D-xylose
recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C
22
D-xylose
-
wild-type enzyme
34
D-xylose
-
pH 7.0, mutant enzyme E277K
500
D-xylose
in 50 mM Bis-Tris propane (pH 8.0), at 50°C
0.00014
L-arabinose
pH 7.5, 70°C, mutant enzyme Y156K
0.00016
L-arabinose
pH 7.5, 70°C, wild-type enzyme
0.00017
L-arabinose
pH 7.5, 70°C, mutant enzyme Y156A
0.118
L-arabinose
-
pH 8.5, reaction with D-glucose
18
L-arabinose
-
pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor
19
L-arabinose
-
pH 8.5, reaction with N-ethylphenazonium ethyl sulfate as electron acceptor
31
L-arabinose
recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C
46
L-arabinose
-
wild-type enzyme
0.63
lactose
pH 5.5, 30°C
7.5
lactose
-
pH 7.0, mutant enzyme E277K
12
lactose
-
pH 7.0, 25°C, linked dimeric enzyme
14.3
lactose
-
pH 7.0, wild-type enzyme
18
lactose
-
N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C
18.9
lactose
recombinant wild-type isozyme PQQGDH-B, pH 7.0
18.9
lactose
-
wild-type isozyme PQQGDH-B, pH 7.0, 25°C
19
lactose
-
recombinant cytochrome c-fusion protein, pH 7.0
20
lactose
-
recombinant Arg-tagged enzyme, and recombinant chimeric mutant enzyme, pH 7.0
25
lactose
recombinant wild-type enzyme, pH 7.0, 25°C
26
lactose
recombinant tagged enzyme, pH 7.0, 25°C
26.7
lactose
-
soluble enzyme
33.6
lactose
-
N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C
33.6
lactose
recombinant mutant N452T, pH 7.0
36
lactose
recombinant triple mutant enzyme, pH 7.0, 25°C
55
lactose
recombinant mutant D167E/N452T, pH 7.0
77
lactose
recombinant mutant D167E, pH 7.0
0.00017
maltose
pH 7.5, 70°C, wild-type enzyme
0.00026
maltose
pH 7.5, 70°C, mutant enzyme Y156K
0.00037
maltose
pH 7.5, 70°C, mutant enzyme Y156A
0.46
maltose
pH 5.5, 30°C
0.59
maltose
-
at pH 5.0 and 45°C
10
maltose
recombinant wild-type enzyme, pH 7.0, 25°C
11
maltose
recombinant tagged enzyme, pH 7.0, 25°C
13
maltose
-
recombinant chimeric mutant enzyme, pH 7.0
13
maltose
recombinant triple mutant enzyme, pH 7.0, 25°C
14
maltose
-
recombinant Arg-tagged enzyme, pH 7.0
14.3
maltose
-
pH 7.0, mutant enzyme E277K
15
maltose
-
recombinant cytochrome c-fusion protein, pH 7.0
16
maltose
-
N462H mutant isozyme PQQGDH-B, pH 7.0, 25°C
16
maltose
recombinant mutant D167E/N452T, pH 7.0
26
maltose
recombinant wild-type isozyme PQQGDH-B, pH 7.0
26
maltose
-
wild-type isozyme PQQGDH-B, pH 7.0, 25°C
30.9
maltose
-
pH 7.0, wild-type enzyme
46.5
maltose
-
N452T mutant isozyme PQQGDH-B, pH 7.0, 25°C
46.5
maltose
recombinant mutant N452T, pH 7.0
156
maltose
recombinant mutant D167E, pH 7.0
600
maltose
in 50 mM Bis-Tris propane (pH 8.0), at 50°C
0.00013
maltotriose
pH 7.5, 70°C, wild-type enzyme
0.00016
maltotriose
pH 7.5, 70°C, mutant enzyme Y156K
0.0006
maltotriose
pH 7.5, 70°C, mutant enzyme Y156A
0.268
N-ethylphenazonium ethyl sulfate
-
pH 8.5, reaction with D-xylose
0.278
N-ethylphenazonium ethyl sulfate
-
pH 8.5, reaction with D-glucose
0.291
N-ethylphenazonium ethyl sulfate
-
pH 8.5, reaction with D-fucose
0.292
N-ethylphenazonium ethyl sulfate
-
pH 8.5, reaction with L-arabinose
0.064
N-methylphenazonium methyl sulfate
-
pH 8.5, reaction with D-galactose
0.156
N-methylphenazonium methyl sulfate
-
pH 8.5, reaction with D-xylose
0.178
N-methylphenazonium methyl sulfate
-
pH 8.5, reaction with D-glucose
0.22
N-methylphenazonium methyl sulfate
-
pH 8.5, reaction with D-fucose
0.362
N-methylphenazonium methyl sulfate
-
pH 8.5, reaction with 2-deoxy-D-glucose
0.4
N-methylphenazonium methyl sulfate
-
with D-glucose as cosubstrate, at pH 5.0 and 45°C
0.074
phenazine methosulfate
-
membrane-bound enzyme form
0.13
phenazine methosulfate
-
pH 8.8, 25°C
1.9
phenazine methosulfate
-
soluble enzyme form
0.00005
pyrroloquinoline quinone
-
,pH 7.0, 25°C, mutant K493A
0.00009
pyrroloquinoline quinone
-
25°C, wild-type enzyme
0.00012
pyrroloquinoline quinone
-
C-terminal periplasmic domain of glucose dehydrogenase
0.00012
pyrroloquinoline quinone
-
25°C, mutant enzyme D730N
0.00014
pyrroloquinoline quinone
-
pH 7.0, 21°C, mutant D466E and D466N
0.00022
pyrroloquinoline quinone
-
pH 7.0, 25°C, mutant W607A
0.0005
pyrroloquinoline quinone
-
25°C, mutant enzyme S357L
0.00064
pyrroloquinoline quinone
-
25°C, mutant enzyme G689D
0.00088
pyrroloquinoline quinone
-
pH 7.0, 25°C, mutant H262A
0.006
pyrroloquinoline quinone
-
pH 7.0, 25°C, mutant W404A
0.016
pyrroloquinoline quinone
-
pH 7.0, 25°C, mutant K493R
0.02
pyrroloquinoline quinone
-
pH 7.0, 25°C, mutant W404F
0.021
pyrroloquinoline quinone
-
25°C, mutant enzyme H775R
0.12
pyrroloquinoline quinone
-
-
0.78
pyrroloquinoline quinone
-
-
0.0001
ubiquinone
-
Km below 0.0002 mM, native enzyme, at pH 7.5 and 28°C
0.00113
ubiquinone
-
recombinant enzyme, at pH 7.5 and 28°C
0.06
ubiquinone Q1
-
pH 8.8, 25°C
0.148
ubiquinone Q1
-
membrane-bound enzyme form
0.178
ubiquinone Q1
-
soluble enzyme form
0.012
ubiquinone Q2
-
membrane-bound enzyme form
0.0177
ubiquinone Q2
-
soluble enzyme form
0.025
ubiquinone Q2
-
wild-type enzyme
0.03
ubiquinone Q2
-
C-terminal periplasmic domain of glucose dehydrogenase
0.04
ubiquinone Q2
-
pH 6.5, 25°C
0.061
ubiquinone Q2
-
pH 8.8, 25°C
0.028
ubiquinone Q4
-
pH 6.5, 25°C
0.031
ubiquinone Q4
-
pH 8.8, 25°C
0.0034
ubiquinone Q6
-
membrane-bound enzyme form
0.0097
ubiquinone Q6
-
pH 8.8, 25°C
0.019
ubiquinone Q6
-
pH 6.5, 25°C
additional information
additional information
kinetics
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additional information
additional information
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electrochemical data and kinetics for quinone derivatives as redocx mediators
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additional information
additional information
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kinetic study, enzyme follows Michaelis-Menten kinetics using artificial electron transfer mediator system based on ruthenium(III) compounds for activity assays
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additional information
additional information
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kinetics, cooperativity in the recombinant chimeric mutant enzyme which possesses only 1 active subunit derived from the wild-type enzyme
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additional information
additional information
metal binding kinetics, wild-type and mutant enzymes, Km for the different substrates of mutant enzymes with different metal ions bound, overview
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additional information
additional information
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metal binding kinetics, wild-type and mutant enzymes, Km for the different substrates of mutant enzymes with different metal ions bound, overview
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additional information
additional information
predicted binding energy of wild-type and mutant enzymes
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additional information
additional information
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predicted binding energy of wild-type and mutant enzymes
-
additional information
additional information
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detailed kinetic analysis of wild-type and mutant enzymes, substrate inhibition and cooperativity effects, overview
-