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Literature summary for 1.1.5.2 extracted from
- Regulation of pyrroloquinoline quinone-dependent glucose dehydrogenase activity in the model rhizosphere-dwelling bacterium Pseudomonas putida KT2440 (2016), Appl. Environ. Microbiol., 82, 4955-4964.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha cells | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
ubiquinone | Km below 0.0002 mM, native enzyme, at pH 7.5 and 28°C | Pseudomonas putida |  |
| 0.00113 | - |
ubiquinone | recombinant enzyme, at pH 7.5 and 28°C | Pseudomonas putida | |
| 2.71 | - |
D-glucose | recombinant enzyme, at pH 7.5 and 28°C | Pseudomonas putida | |
| 4.91 | - |
D-glucose | native enzyme, at pH 7.5 and 28°C | Pseudomonas putida | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Pseudomonas putida | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + ubiquinone | Pseudomonas putida | - |
D-glucono-1,5-lactone + ubiquinol | - |
? | |
| D-glucose + ubiquinone | Pseudomonas putida KT 2240 | - |
D-glucono-1,5-lactone + ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida KT 2240 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Pseudomonas putida KT 2240 | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-glucose + ubiquinone | - |
Pseudomonas putida | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| D-glucose + ubiquinone | - |
Pseudomonas putida KT 2240 | D-glucono-1,5-lactone + ubiquinol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GCD | - |
Pseudomonas putida |
| GDH | - |
Pseudomonas putida |
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Release 2023.1 (January 2023)
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