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Literature summary for 1.1.5.2 extracted from

  • Igarashi, S.; Hirokawa, T.; Sode, K.
    Engineering PQQ glucose dehydrogenase with improved substrate specificity. Site-directed mutagenesis studies on the active center of PQQ glucose dehydrogenase (2004), Biomol. Eng., 21, 81-89.
    View publication on PubMed
Search for more literature for 1.1.5.2

Application

Application Comment Organism
biotechnology engineering PQQ glucose dehydrogenase with improved substrate specificity Acinetobacter calcoaceticus

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant isozyme PQQGDH-B Acinetobacter calcoaceticus

Protein Variants

Protein Variants Comment Organism
D167A site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
D167C site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
D167E site-directed mutagenesis, substrate binding residue mutation, slightly reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
D167E/N452T site-directed mutagenesis, reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
D167G site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
D167H site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
D167K site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
D167N site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
D167Q site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
D167R site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
D167S site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
D167V site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
D167W site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
D167Y site-directed mutagenesis, substrate binding residue mutation, reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
H168C site-directed mutagenesis, catalytic residue mutation, highly reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
H168Q site-directed mutagenesis, catalytic residue mutation, nearly inactive mutant Acinetobacter calcoaceticus
K166E site-directed mutagenesis, substrate binding residue mutation, altered substrate specificty compared to the wild-type enzyme Acinetobacter calcoaceticus
K166G site-directed mutagenesis, substrate binding residue mutation, altered substrate specificty compared to the wild-type enzyme Acinetobacter calcoaceticus
K166I site-directed mutagenesis, substrate binding residue mutation, altered substrate specificty compared to the wild-type enzyme Acinetobacter calcoaceticus
additional information engineering PQQ glucose dehydrogenase with improved substrate specificity Acinetobacter calcoaceticus
N452T site-directed mutagenesis, reduced activity compared to the wild-type enzyme Acinetobacter calcoaceticus
Q169E site-directed mutagenesis, substrate binding residue mutation, altered substrate specificty compared to the wild-type enzyme Acinetobacter calcoaceticus
Q169K site-directed mutagenesis, substrate binding residue mutation, altered substrate specificty compared to the wild-type enzyme Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7
-
 D-galactose recombinant mutant N452T, pH 7.0 Acinetobacter calcoaceticus
5.3
-
 D-galactose recombinant wild-type isozyme PQQGDH-B, pH 7.0 Acinetobacter calcoaceticus
12.5
-
 D-glucose recombinant mutant N452T, pH 7.0 Acinetobacter calcoaceticus
14
-
 cellobiose recombinant wild-type isozyme PQQGDH-B and mutant N452T, pH 7.0 Acinetobacter calcoaceticus
16
-
 cellobiose recombinant mutant D167E/N452T, pH 7.0 Acinetobacter calcoaceticus
16
-
 maltose recombinant mutant D167E/N452T, pH 7.0 Acinetobacter calcoaceticus
17
-
 cellobiose recombinant mutant D167E, pH 7.0 Acinetobacter calcoaceticus
18.9
-
 lactose recombinant wild-type isozyme PQQGDH-B, pH 7.0 Acinetobacter calcoaceticus
25
-
 D-glucose recombinant wild-type isozyme PQQGDH-B, pH 7.0 Acinetobacter calcoaceticus
26
-
 maltose recombinant wild-type isozyme PQQGDH-B, pH 7.0 Acinetobacter calcoaceticus
27.6
-
 3-O-methyl-D-glucose recombinant mutant N452T, pH 7.0 Acinetobacter calcoaceticus
28.7
-
 3-O-methyl-D-glucose recombinant wild-type isozyme PQQGDH-B, pH 7.0 Acinetobacter calcoaceticus
33.6
-
 lactose recombinant mutant N452T, pH 7.0 Acinetobacter calcoaceticus
35.5
-
 allose recombinant wild-type isozyme PQQGDH-B, pH 7.0 Acinetobacter calcoaceticus
38.7
-
 allose recombinant mutant N452T, pH 7.0 Acinetobacter calcoaceticus
46.5
-
 maltose recombinant mutant N452T, pH 7.0 Acinetobacter calcoaceticus
48
-
 D-glucose recombinant mutant D167E/N452T, pH 7.0 Acinetobacter calcoaceticus
55
-
 D-glucose recombinant mutant D167E, pH 7.0 Acinetobacter calcoaceticus
55
-
 lactose recombinant mutant D167E/N452T, pH 7.0 Acinetobacter calcoaceticus
77
-
 lactose recombinant mutant D167E, pH 7.0 Acinetobacter calcoaceticus
99
-
 3-O-methyl-D-glucose recombinant mutant D167E, pH 7.0 Acinetobacter calcoaceticus
145
-
 D-galactose recombinant mutant D167E/N452T, pH 7.0 Acinetobacter calcoaceticus
154
-
 D-glucose recombinant mutant H168Q, pH 7.0 Acinetobacter calcoaceticus
156
-
 maltose recombinant mutant D167E, pH 7.0 Acinetobacter calcoaceticus
182
-
 allose recombinant mutant D167E/N452T, pH 7.0 Acinetobacter calcoaceticus
193
-
 D-glucose recombinant mutant H168C, pH 7.0 Acinetobacter calcoaceticus
198
-
 3-O-methyl-D-glucose recombinant mutant D167E/N452T, pH 7.0 Acinetobacter calcoaceticus
199
-
 allose recombinant mutant D167E, pH 7.0 Acinetobacter calcoaceticus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble isozyme PQQGDH-B Acinetobacter calcoaceticus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus P13650 isozyme PQQGDH-B
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus 2-deoxy-D-glucono-1,5-lactone + ?
-
 ?
3-O-methyl-D-glucose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + ?
-
 ?
allose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus ?
-
 ?
cellobiose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus ?
-
 ?
D-galactose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus D-galactono-1,5-lactone + ?
-
 ?
D-glucose + 2,6-dichlorophenolindolphenol best substrate Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
D-glucose + ubiquinone best substrate Acinetobacter calcoaceticus D-glucono-1,5-lactone + ubiquinol
-
 ?
D-mannose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus ?
-
 ?
D-xylose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus D-xylono-1,5-lactone + ?
-
 ?
lactose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus ?
-
 ?
maltose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus ?
-
 ?
additional information substrate specificities of recombinant wild-type and mutant enzymes, overview Acinetobacter calcoaceticus ?
-
 ?

Synonyms

Synonyms Comment Organism
PQQ glucose dehydrogenase
-
 Acinetobacter calcoaceticus
PQQGDH-B
-
 Acinetobacter calcoaceticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 thermal stability of wild-type and mutant isozymes PQQGDH-B, overview Acinetobacter calcoaceticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 D-glucose recombinant mutant H168Q, pH 7.0 Acinetobacter calcoaceticus
2.5
-
 D-glucose recombinant mutant H168C, pH 7.0 Acinetobacter calcoaceticus
65
-
 maltose recombinant mutant D167E/N452T, pH 7.0 Acinetobacter calcoaceticus
72
-
 D-galactose recombinant mutant N452T, pH 7.0 Acinetobacter calcoaceticus
73
-
 allose recombinant mutant D167E/N452T, pH 7.0 Acinetobacter calcoaceticus
89
-
 D-galactose recombinant mutant D167E/N452T, pH 7.0 Acinetobacter calcoaceticus
167
-
 lactose recombinant mutant D167E/N452T, pH 7.0 Acinetobacter calcoaceticus
215
-
 3-O-methyl-D-glucose recombinant mutant D167E/N452T, pH 7.0 Acinetobacter calcoaceticus
226
-
 cellobiose recombinant mutant D167E/N452T, pH 7.0 Acinetobacter calcoaceticus
232
-
 D-galactose recombinant wild-type isozyme PQQGDH-B, pH 7.0 Acinetobacter calcoaceticus
436
-
 maltose recombinant mutant D167E, pH 7.0 Acinetobacter calcoaceticus
478
-
 lactose recombinant mutant D167E, pH 7.0 Acinetobacter calcoaceticus
541
-
 3-O-methyl-D-glucose recombinant mutant D167E, pH 7.0 Acinetobacter calcoaceticus
558
-
 allose recombinant mutant D167E, pH 7.0 Acinetobacter calcoaceticus
949
-
 allose recombinant mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1002
-
 maltose recombinant mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1038
-
 lactose recombinant mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1060
-
 cellobiose recombinant mutant D167E, pH 7.0 Acinetobacter calcoaceticus
1073
-
 cellobiose recombinant mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1193
-
 D-glucose recombinant mutant D167E/N452T, pH 7.0 Acinetobacter calcoaceticus
1253
-
 3-O-methyl-D-glucose recombinant mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1355
-
 cellobiose recombinant wild-type isozyme PQQGDH-B, pH 7.0 Acinetobacter calcoaceticus
1659
-
 lactose recombinant wild-type isozyme PQQGDH-B, pH 7.0 Acinetobacter calcoaceticus
1724
-
 D-glucose recombinant mutant D167E, pH 7.0 Acinetobacter calcoaceticus
1791
-
 D-glucose recombinant mutant N452T, pH 7.0 Acinetobacter calcoaceticus
1930
-
 maltose recombinant wild-type isozyme PQQGDH-B, pH 7.0 Acinetobacter calcoaceticus
2509
-
 allose recombinant wild-type isozyme PQQGDH-B, pH 7.0 Acinetobacter calcoaceticus
3011
-
 3-O-methyl-D-glucose recombinant wild-type isozyme PQQGDH-B, pH 7.0 Acinetobacter calcoaceticus
3860
-
 D-glucose recombinant wild-type isozyme PQQGDH-B, pH 7.0 Acinetobacter calcoaceticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone i.e. PQQ, dependent on Acinetobacter calcoaceticus