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Literature summary for 1.1.5.2 extracted from
- Characterization of the membrane quinoprotein glucose dehydrogenase from Escherichia coli and characterization of a site-directed mutant in which histidine-262 has been changed to tyrosine (1999), Biochem. J., 340, 639-647.
No PubMed abstract available
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H262Y | greatly diminished catalytic efficiency for all substrates, rate of electron transfer to oxygen is unaffected, 230fold increased Km value for glucose | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
6-deoxy-D-glucose | wild-type enzyme | Escherichia coli |  |
| 1.6 | - |
2-deoxy-D-glucose | wild-type enzyme | Escherichia coli | |
| 2.1 | - |
D-glucose | wild-type enzyme | Escherichia coli | |
| 2.5 | - |
D-allose | wild-type enzyme | Escherichia coli | |
| 8.3 | - |
D-fucose | wild-type enzyme | Escherichia coli | |
| 9.5 | - |
2-amino-D-glucose | wild-type enzyme | Escherichia coli | |
| 10.8 | - |
3-deoxy-D-glucose | wild-type enzyme | Escherichia coli | |
| 17.7 | - |
D-melibiose | wild-type enzyme | Escherichia coli | |
| 22 | - |
D-xylose | wild-type enzyme | Escherichia coli | |
| 32 | - |
2-deoxy-D-glucose | mutant enzyme H262 | Escherichia coli | |
| 39 | - |
D-galactose | wild-type enzyme | Escherichia coli | |
| 46 | - |
L-arabinose | wild-type enzyme | Escherichia coli | |
| 78 | - |
D-mannose | wild-type enzyme | Escherichia coli | |
| 79 | - |
3-O-methyl-D-glucose | wild-type enzyme | Escherichia coli | |
| 100 | - |
L-Lyxose | wild-type enzyme | Escherichia coli | |
| 110 | - |
D-ribose | wild-type enzyme | Escherichia coli | |
| 460 | - |
D-glucose | mutant enzyme H262Y | Escherichia coli | |
| 810 | - |
D-allose | mutant enzyme H262Y | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Escherichia coli | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| wild-type and mutant enzyme H262Y | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-amino-D-glucose + pyrroloquinoline quinone | - |
Escherichia coli | 2-amino-D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| 2-deoxy-D-glucose + pyrroloquinoline quinone | - |
Escherichia coli | 2-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| 3-deoxy-D-glucose + pyrroloquinoline quinone | - |
Escherichia coli | 3-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| 3-O-methyl-D-glucose + pyrroloquinoline quinone | - |
Escherichia coli | 3-O-methyl-D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| 6-deoxy-D-glucose + pyrroloquinoline quinone | - |
Escherichia coli | 6-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| D-allose + pyrroloquinoline quinone | - |
Escherichia coli | D-allono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| D-fucose + pyrrolquinoline quinone | - |
Escherichia coli | 6-deoxy-D-galactono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| D-galactose + pyrroloquinoline quinone | - |
Escherichia coli | D-galactono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| D-glucose + pyrroloquinoline quinone | - |
Escherichia coli | D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| D-mannose + pyrroloquinoline quinone | - |
Escherichia coli | D-mannono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| D-melibiose + pyrroloquinoline quinone | - |
Escherichia coli | ? | - |
? | |
| D-ribose + pyrroloquinoline quinone | - |
Escherichia coli | D-ribono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| D-xylose + pyrroloquinoline quinone | - |
Escherichia coli | D-xylono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| L-arabinose + pyrroloquinoline quinone | - |
Escherichia coli | L-arabino-1,5-lactone + pyrroloquinoline quinol | - |
? | |
| L-lyxose + pyrroloquinoline quinone | - |
Escherichia coli | ? | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | prosthetic group | Escherichia coli | |
| pyrroloquinoline quinone | during the processing of pyrroloquinoline quinone into the apoenzyme to give active enzyme, its affinity is markedly dependent on the pH, four groups with pK values between pH 7 and pH 8 are involved | Escherichia coli | |
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