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Literature summary for 1.1.5.2 extracted from

  • Laurinavicius, V.; Razumiene, J.; Kurtinaitiene, B.; Gureviciene, V.; Marcinkeviciene, L.; Bachmatova, I.
    Comparative characterization of soluble and membrane-bound PQQ-glucose dehydrogenases (2003), Biologia (Bratisl. ), 2, 31-34.
No PubMed abstract available
Search for more literature for 1.1.5.2

Application

Application Comment Organism
diagnostics purified enzyme is immobilized on carbon electrodes modified with 4-ferrocenylphenol, 4-(4-ferrocenylimino-methyl)-phenol, or 4-ferrocenylnitrophenol, for use as glucose biosensors, kinetic behaviour during immobilization Erwinia sp.
diagnostics purified enzyme is immobilized on carbon electrodes modified with 4-ferrocenylphenol, 4-(4-ferrocenylimino-methyl)phenol, or 4-ferrocenylnitrophenol, for use as glucose biosensors, kinetic behaviour during immobilization Acinetobacter calcoaceticus

General Stability

General Stability Organism
the membrane-bound enzyme is more stable than the soluble form Erwinia sp.
the soluble enzyme is less stable than the membrane-bound form Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.73
-
 4-(4-ferrocenylimino-methyl)phenol s-GDH Acinetobacter calcoaceticus
0.83
-
 4-ferrocenylphenol m-GDH Erwinia sp.
0.89
-
 4-ferrocenylnitrophenol s-GDH Acinetobacter calcoaceticus
1
-
 4-ferrocenylphenol s-GDH Acinetobacter calcoaceticus
2.43
-
 4-(4-ferrocenylimino-methyl)phenol m-GDH Erwinia sp.
2.73
-
 4-ferrocenylnitrophenol m-GDH Erwinia sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound enzyme m-GDH Erwinia sp. 16020
-
soluble enzyme s-GDH Acinetobacter calcoaceticus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Acinetobacter calcoaceticus
Ca2+ required Erwinia sp.

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
 enzyme s-GDH
-
Acinetobacter calcoaceticus L.M.D. 79.41
-
 enzyme s-GDH
-
Erwinia sp.
-
 m-GDH
-
Erwinia sp. 34-1
-
 m-GDH
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
 purified native m-GDH Erwinia sp.
250
-
 purified native s-GDH Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + ubiquinone
-
 Acinetobacter calcoaceticus ? + ubiquinol
-
 ?
cellobiose + ubiquinone
-
 Acinetobacter calcoaceticus L.M.D. 79.41 ? + ubiquinol
-
 ?
D-galactose + ubiquinone
-
 Acinetobacter calcoaceticus D-galactono-1,5-lactone + ubiquinol
-
 ?
D-galactose + ubiquinone
-
 Erwinia sp. D-galactono-1,5-lactone + ubiquinol
-
 ?
D-galactose + ubiquinone
-
 Erwinia sp. 34-1 D-galactono-1,5-lactone + ubiquinol
-
 ?
D-galactose + ubiquinone
-
 Acinetobacter calcoaceticus L.M.D. 79.41 D-galactono-1,5-lactone + ubiquinol
-
 ?
D-glucose + 4-(4-ferrocenylimino-methyl)phenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
D-glucose + 4-(4-ferrocenylimino-methyl)phenol
-
 Erwinia sp. D-glucono-1,5-lactone + ?
-
 r
D-glucose + 4-(4-ferrocenylimino-methyl)phenol
-
 Erwinia sp. 34-1 D-glucono-1,5-lactone + ?
-
 r
D-glucose + 4-(4-ferrocenylimino-methyl)phenol
-
 Acinetobacter calcoaceticus L.M.D. 79.41 D-glucono-1,5-lactone + ?
-
 ?
D-glucose + 4-ferrocenylnitrophenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
D-glucose + 4-ferrocenylnitrophenol
-
 Erwinia sp. D-glucono-1,5-lactone + ?
-
 ?
D-glucose + 4-ferrocenylnitrophenol
-
 Erwinia sp. 34-1 D-glucono-1,5-lactone + ?
-
 ?
D-glucose + 4-ferrocenylphenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
D-glucose + 4-ferrocenylphenol
-
 Erwinia sp. D-glucono-1,5-lactone + ?
-
 ?
D-glucose + ubiquinone best substrate Acinetobacter calcoaceticus D-glucono-1,5-lactone + ubiquinol
-
 ?
D-glucose + ubiquinone best substrate Erwinia sp. D-glucono-1,5-lactone + ubiquinol
-
 ?
D-glucose + ubiquinone best substrate Erwinia sp. 34-1 D-glucono-1,5-lactone + ubiquinol
-
 ?
D-glucose + ubiquinone best substrate Acinetobacter calcoaceticus L.M.D. 79.41 D-glucono-1,5-lactone + ubiquinol
-
 ?
L-arabinose + ubiquinone
-
 Acinetobacter calcoaceticus L-arabino-1,5-lactone + ubiquinol
-
 ?
L-arabinose + ubiquinone
-
 Erwinia sp. L-arabino-1,5-lactone + ubiquinol
-
 ?
lactose + ubiquinone
-
 Acinetobacter calcoaceticus ? + ubiquinol
-
 ?
maltose + ubiquinone
-
 Acinetobacter calcoaceticus ? + ubiquinol
-
 ?
additional information substrate specificity of native and immobilized enzyme Acinetobacter calcoaceticus ?
-
 ?
additional information substrate specificity of native and immobilized enzyme, maltose is a poor substrate Erwinia sp. ?
-
 ?
additional information substrate specificity of native and immobilized enzyme, maltose is a poor substrate Erwinia sp. 34-1 ?
-
 ?
additional information substrate specificity of native and immobilized enzyme Acinetobacter calcoaceticus L.M.D. 79.41 ?
-
 ?

Synonyms

Synonyms Comment Organism
m-GDH
-
 Erwinia sp.
PQQ-GDH
-
 Acinetobacter calcoaceticus
PQQ-GDH
-
 Erwinia sp.
PQQ-glucose dehydrogenase
-
 Acinetobacter calcoaceticus
PQQ-glucose dehydrogenase
-
 Erwinia sp.
s-GDH
-
 Acinetobacter calcoaceticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pH-dependence of native and immobilized enzyme Acinetobacter calcoaceticus
additional information
-
 pH-dependence of native and immobilized enzyme Erwinia sp.
5.5
-
 native m-GDH Erwinia sp.
6 9 immobilized s-GDH, broad maximum Acinetobacter calcoaceticus
6
-
 immobilized m-GDH Erwinia sp.
7
-
 native s-GDH Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone i.e. PQQ, dependent on Acinetobacter calcoaceticus
pyrroloquinoline quinone i.e. PQQ, dependent on Erwinia sp.