Protein Variants | Comment | Organism |
---|---|---|
D276E | drastic decrease in EDTA tolerance | Acinetobacter calcoaceticus |
E277A | decreased Km value for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme | Acinetobacter calcoaceticus |
E277D | decreased Km value for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme | Acinetobacter calcoaceticus |
E277G | drastic decrease in EDTA tolerance | Acinetobacter calcoaceticus |
E277H | decreased Km values for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme | Acinetobacter calcoaceticus |
E277K | decreased Km value for glucose and altered substrate specificity, significantly increased catalytic efficiency compared with the wild-type enzyme | Acinetobacter calcoaceticus |
E277N | decreased Km values for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme | Acinetobacter calcoaceticus |
E277Q | decreased Km values for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme | Acinetobacter calcoaceticus |
E277V | decreased Km values for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme | Acinetobacter calcoaceticus |
N275E | drastic decrease in EDTA tolerance | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
D-glucose | pH 7.0, mutant enzyme E277G | Acinetobacter calcoaceticus | |
1.2 | - |
D-glucose | mutant E277N | Acinetobacter calcoaceticus | |
1.5 | - |
D-glucose | mutant enzyme E277A | Acinetobacter calcoaceticus | |
2.5 | - |
D-glucose | pH 7.0, mutant enzyme E277V | Acinetobacter calcoaceticus | |
4.3 | - |
D-glucose | pH 7.0, mutant enzyme E277Q | Acinetobacter calcoaceticus | |
6.8 | - |
D-galactose | pH 7.0, wild-type enzyme and mutant enzyme E277K | Acinetobacter calcoaceticus | |
7 | - |
D-glucose | pH 7.0, mutant enzyme I278F | Acinetobacter calcoaceticus | |
7.4 | - |
D-glucose | pH 7.0, mutant enzyme E277D | Acinetobacter calcoaceticus | |
7.5 | - |
lactose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
7.7 | - |
D-glucose | pH 7.0, mutant enzyme E277H | Acinetobacter calcoaceticus | |
8.8 | - |
D-glucose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
8.9 | - |
D-glucose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
14.3 | - |
maltose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
14.3 | - |
lactose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
14.3 | - |
D-xylose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
15.7 | - |
D-glucose | pH 7.0, mutant enzyme N279H | Acinetobacter calcoaceticus | |
21 | - |
allose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
22 | - |
D-mannose | pH 7.0, wild-type enzyme and mutant enzyme E277K | Acinetobacter calcoaceticus | |
24 | - |
D-glucose | pH 7.0, mutant enzyme D275E and D276E | Acinetobacter calcoaceticus | |
26 | - |
D-glucose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
27 | - |
3-O-methyl-D-glucose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
29 | - |
D-allose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
30.9 | - |
maltose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
34 | - |
D-xylose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
46 | - |
3-O-methyl-D-glucose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
88 | - |
2-deoxy-D-glucose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
90 | - |
2-deoxy-D-glucose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
expression in Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus | 2-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
3-O-methyl-D-glucose + pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus | 3-O-methyl-D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
D-allose + pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus | D-allono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
D-galactose + pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus | D-galactono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
D-glucose + 2,6-dichlorophenol-indophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
D-glucose + phenazine methosulfate | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
D-mannose + pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus | D-mannono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
D-xylose + pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus | D-xylono-1,5-lactone + pyrroloquinoline quinol | - |
? | |
lactose + pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus | ? | - |
? | |
maltose + pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
t1/2: 10 min for wild-type enzyme and mutant enzyme E277K, 4 min for mutant enzymes E277Q and N279H, 25 min for mutant enzyme I278F, less than 2 min for mutant enzymes E277G, E277A, E277D, E277H, E277N, E277V, D275E and D276E | Acinetobacter calcoaceticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
121 | - |
D-galactose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
201 | - |
D-xylose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
267 | - |
D-mannose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
331 | - |
2-deoxy-D-glucose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
630 | - |
D-galactose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
669 | - |
lactose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
678 | - |
D-xylose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
785 | - |
maltose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
861 | - |
D-mannose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
1020 | - |
maltose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
1060 | - |
2-deoxy-D-glucose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
1440 | - |
D-allose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
1450 | - |
3-O-methyl-D-glucose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
1800 | - |
lactose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
3070 | - |
D-glucose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
3200 | - |
3-O-methyl-D-glucose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus | |
3440 | - |
D-glucose | pH 7.0, wild-type enzyme | Acinetobacter calcoaceticus | |
4560 | - |
D-allose | pH 7.0, mutant enzyme E277K | Acinetobacter calcoaceticus |