Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D730A | low glucose oxidase activity without influence on the affinity for pyrroloquinoline quinone, Mg2+ or substrate | Escherichia coli |
D730N | low glucose oxidase activity without influence on the affinity for pyrroloquinoline quinone, Mg2+ or substrate | Escherichia coli |
D730R | reduced affnity for pyrroloquinoline quinone | Escherichia coli |
E742G/P757L | slightly higher Km value for Mg2+ | Escherichia coli |
G689D | significantly increased Km for pyrroloquinoline quinone, slightly higher Km value for Mg2+ | Escherichia coli |
H775A | pronounced reduction of affinity for the prosthetic group pyrroloquinoline quinone | Escherichia coli |
H775R | pronounced reduction of affinity for the prosthetic group pyrroloquinoline quinone, 230fold higher Km than wild-type enzyme | Escherichia coli |
S357L | significantly increased Km for pyrroloquinoline quinone, slightly higher Km value for Mg2+ | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00009 | - |
pyrroloquinoline quinone | 25°C, wild-type enzyme | Escherichia coli | |
0.00012 | - |
pyrroloquinoline quinone | 25°C, mutant enzyme D730N | Escherichia coli | |
0.0005 | - |
pyrroloquinoline quinone | 25°C, mutant enzyme S357L | Escherichia coli | |
0.00064 | - |
pyrroloquinoline quinone | 25°C, mutant enzyme G689D | Escherichia coli | |
0.021 | - |
pyrroloquinoline quinone | 25°C, mutant enzyme H775R | Escherichia coli | |
0.8 | - |
D-glucose | 25°C, mutant enzyme D730 N | Escherichia coli | |
0.91 | - |
D-glucose | 25°C, wild-type enzyme | Escherichia coli | |
1 | - |
D-glucose | 25°C, mutant enzyme S357L | Escherichia coli | |
1.3 | - |
D-glucose | 25°C, mutant enzyme H775R | Escherichia coli | |
1.4 | - |
D-glucose | 25°C, mutant enzyme G689D | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Km: 0.022 mM for the wild-type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant enzymes | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + pyrroloquinoline quinone | - |
Escherichia coli | D-glucono-1,5-lactone + pyrroloquinoline quinol | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | prosthetic group | Escherichia coli |