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medicine
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insertion of a calmodulin domain into enzyme results in a selective Ca2+ biosensor that could be used to rapidly measure Ca2+ concentrations in human biological fluids
analysis
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application of mutant enzyme S231K as a glucose sensor constituent. The mutant has more than 8fold increase in its half-life during the thermal inactivation at 55 C compared with the wild-type enzyme and retains catalytic activity similar to the wild-type enzyme
analysis
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apoenzyme is used as a biological test system for the detection of very low amounts of pyrroloquinoline quinone
analysis
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apoenzyme is used as a biological test system for the detection of very low amounts of pyrroloquinoline quinone
analysis
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enzyme is used as coupling enzyme for monitoring carbohydrate-transport reactions, the method is particularly suited for determining transport reactions that are not coupled to any form of metabolic energy such as uniport reactions, or for characterizing mutant proteins with a defective energy-coupling mechanism or system with high-affinity constants for sugars
analysis
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because of its high turnover number, PQQ-GDH is proposed as an enzyme label for the development of sensitive electrochemical enzyme-amplified bioaffinity assays. It has, for example, been applied to the amperometric detection of DNA hybrids or sandwich DNA aptamers at the surface of a carbon electrode
analysis
its high stability at high temperature makes this enzyme potentially useful for applications in biosensors or biofuel cells
analysis
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its high stability at high temperature makes this enzyme potentially useful for applications in biosensors or biofuel cells
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biofuel production
construction of a long-life biofuel cell using a hyperthermophilic enzyme. For the cathode, the multicopper oxidase from the hyperthermophilic archaeon Pyrobaculum aerophilum is used, which catalyzes a four-electron reduction, and, for the anode, the PQQ-dependent glucose dehydrogenase from Pyrobaculum aerophilum is used. When the enzymes are used as electrodes, oriented with carbon nanotubes in a highly organized manner, the maximum output is 0.011 mW at 0.2 V. This output can be maintained 70% after 14 days
biofuel production
its high stability at high temperature makes this enzyme potentially useful for applications in biosensors or biofuel cells
biofuel production
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construction of a long-life biofuel cell using a hyperthermophilic enzyme. For the cathode, the multicopper oxidase from the hyperthermophilic archaeon Pyrobaculum aerophilum is used, which catalyzes a four-electron reduction, and, for the anode, the PQQ-dependent glucose dehydrogenase from Pyrobaculum aerophilum is used. When the enzymes are used as electrodes, oriented with carbon nanotubes in a highly organized manner, the maximum output is 0.011 mW at 0.2 V. This output can be maintained 70% after 14 days
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biofuel production
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its high stability at high temperature makes this enzyme potentially useful for applications in biosensors or biofuel cells
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biotechnology
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bioengineering of water-soluble isozyme PQQGDH-B production at industrial level
biotechnology
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engineering of the soluble enzyme GDH-B to enable the electron transfer to the electrode in absence of artificial electron mediator by mimicking the domain structure of the quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni, which is composed of a PQQ-containing catalytic domain and a cytochrome c domain
biotechnology
engineering PQQ glucose dehydrogenase with improved substrate specificity
biotechnology
enzyme has a great potential for application as glucose sensor constituent
biotechnology
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optimization of an expression system using Pichia pastoris for use in industrial level production
biotechnology
surface charge engineering of the enzyme for optimization of downstream processing in large scale enzyme production
biotechnology
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the enzyme is used for glucose biosensor diagnosis
biotechnology
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a Glucose sensitive biosensor containing GDH immobilized on Prussian blue (PB)-modified graphite electrode is designed. Properties of the biosensor are investigated in the cathodic and anodic response detection regions. It is shown, that anodic response of the biosensor is sum of two signals: direct electron transport from reduced pyrroloquinoline quinine to the electrode and by formation of the pyrroloquinoline quinone-oxygen-Prussion blue-carbon ternary complex. Cathodic response of the biosensor is based on the oxidation of the reduced pyrroloquinoline quinone by Prussian blue-oxygen-Prussian blue complex. Electrochemical regeneration of the enzyme does not produce free hydrogen peroxide
biotechnology
application as biosensor
biotechnology
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engineering of the soluble enzyme GDH-B to enable the electron transfer to the electrode in absence of artificial electron mediator by mimicking the domain structure of the quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni, which is composed of a PQQ-containing catalytic domain and a cytochrome c domain
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diagnostics
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enzyme is industrially used as glucose sensor with high catalytic activity and insensitivity to oxygen
diagnostics
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purified enzyme is immobilized on carbon electrodes modified with 4-ferrocenylphenol, 4-(4-ferrocenylimino-methyl)-phenol, or 4-ferrocenylnitrophenol, for use as glucose biosensors, kinetic behaviour during immobilization
diagnostics
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purified enzyme is immobilized on carbon electrodes modified with 4-ferrocenylphenol, 4-(4-ferrocenylimino-methyl)phenol, or 4-ferrocenylnitrophenol, for use as glucose biosensors, kinetic behaviour during immobilization
diagnostics
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the enzyme is attractive for application in glucose detection in clinic diagnosis and industrial bioprocess controls
diagnostics
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purified enzyme is immobilized on carbon electrodes modified with 4-ferrocenylphenol, 4-(4-ferrocenylimino-methyl)-phenol, or 4-ferrocenylnitrophenol, for use as glucose biosensors, kinetic behaviour during immobilization
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diagnostics
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enzyme is industrially used as glucose sensor with high catalytic activity and insensitivity to oxygen
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diagnostics
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the enzyme is attractive for application in glucose detection in clinic diagnosis and industrial bioprocess controls
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diagnostics
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purified enzyme is immobilized on carbon electrodes modified with 4-ferrocenylphenol, 4-(4-ferrocenylimino-methyl)phenol, or 4-ferrocenylnitrophenol, for use as glucose biosensors, kinetic behaviour during immobilization
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