Information on EC 5.3.1.8 - Mannose-6-phosphate isomerase

5.3.1.8

-

Mannose-6-phosphate isomerase

GO:0004476

D-Mannose 6-phosphate = D-fructose 6-phosphate

-

-

-

-

D-Mannose 6-phosphate = D-fructose 6-phosphate

analysis of enzyme-substrate and enzyme-inhibitor complexes by ESI-FTICR mass spectrometry

Escherichia coli

-

660706

D-Mannose 6-phosphate = D-fructose 6-phosphate

cis-enediol mechanism

Pyrobaculum aerophilum

-, Q8ZWV0

661100

D-Mannose 6-phosphate = D-fructose 6-phosphate

mechanism does not involve trans-enediol. Instead, activity may result from additional space in the active site which permits rotation of the C2-C3 bond

Pyrobaculum aerophilum

Q8ZWV0

662267

D-Mannose 6-phosphate = D-fructose 6-phosphate

hydride transfer mechanism of a alpha-hydrogen bond between the C1 and C2 positions of substrate. Mechanism involves Zn2+ mediating the movement of a proton between O1 and O2, and the hydrophobic environment formed in part by T278 promoting transfer of a hydride ion

Homo sapiens

-

681498

D-Mannose 6-phosphate = D-fructose 6-phosphate

reaction mechanism, overview. Zn2+ binding induces structural order in the loop consisting of residues 50-54. The metal atom appears to play a role in substrate binding and is probably also important for maintaining the architecture of the active site. Isomerization probably follows a cis-enediol mechanism, overall folding pattern of the central catalytic domain, overview

Salmonella enterica subsp. enterica serovar Typhimurium

P25081

701472

aldose-ketose-isomerization

Thermus thermophilus

Q5SIM4

-

714372

aldose-ketose-isomerization

Thermus thermophilus KCCM 40879

-

-

-

intramolecular oxidoreduction

-

-

-

-

isomerization

-

-

-

-

D-mannose degradation

-

MANNCAT-PWY

GDP-mannose biosynthesis

-

PWY-5659

L-ascorbate biosynthesis I (L-galactose pathway)

-

PWY-882

mannan degradation

-

PWY-7456

mannitol biosynthesis

-

PWY-3881

mannitol degradation II

-

PWY-3861

Fructose and mannose metabolism

00051

-

Amino sugar and nucleotide sugar metabolism

00520

-

Metabolic pathways

01100

-

Biosynthesis of secondary metabolites

01110

-

D-mannose-6-phosphate aldose-ketose-isomerase

A zinc protein.

BceAJ

Burkholderia cenocepacia

-

-

690577

becA

Burkholderia cepacia

Q06XM8

-

671785

becA

Burkholderia cepacia IST408

Q06XM8

-

-

D-mannose-6-phosphate ketol-isomerase

-

-

-

-

GTMpi

Geobacillus thermodenitrificans

-

-

702817

Isomerase, mannose phosphate

-

-

-

-

ManA

Photorhabdus luminescens

-

-

713855

ManA

Photorhabdus luminescens TT01

-

-

-

Mannose phosphate isomerase

-

-

-

-

mannose-6-phosphate isomerase

Thermus thermophilus

Q5SIM4

-

714372

mannose-6-phosphate isomerase

Thermus thermophilus KCCM 40879

Q5SIM4

-

-

MPI

Geobacillus thermodenitrificans

-

-

701812

MPI

Mus musculus

-

-

662458

MPI

Salmonella enterica subsp. enterica serovar Typhimurium

P25081

-

701472

MPI

Thermus thermophilus

-

-

713854

MPI

Thermus thermophilus

Q5SIM4

-

714372

MPI

Thermus thermophilus KCCM 40879

-, Q5SIM4

-

-

PHM

Sulfolobus solfataricus

-

-

680450

PHM

Sulfolobus solfataricus P1

-

-

-

Phosphohexoisomerase

-

-

-

-

Phosphohexomutase

-

-

-

-

Phosphohexomutase

Sulfolobus solfataricus

-

-

680450

Phosphohexomutase

Sulfolobus solfataricus P1

-

-

-

Phosphomannoisomerase

-

-

-

-

Phosphomannose isomerase

-

-

-

-

Phosphomannose isomerase

Alcaligenes sp.

-

-

701879

Phosphomannose isomerase

Alcaligenes sp. CGMCC2428

-

-

-

Phosphomannose isomerase

Arabidopsis thaliana

Q9M884

-

700488

Phosphomannose isomerase

Aspergillus fumigatus

Q66WM4

-

705568

Phosphomannose isomerase

Aspergillus fumigatus YJ-407

Q66WM4

-

-

Phosphomannose isomerase

Brassica rapa subsp. chinensis

G1E7A7

-

728283

Phosphomannose isomerase

Escherichia coli

-

-

702663, 727158, 728469

Phosphomannose isomerase

Photorhabdus luminescens

-

-

713855

Phosphomannose isomerase

Photorhabdus luminescens TT01

-

-

-

Phosphomannose isomerase

Placopecten magellanicus

-

-

705394

Phosphomannose isomerase

Pyrococcus furiosus

-

type II phosphomannose isomerase, bifunctional phosphomannose isomerase/GDP-mannose pyrophosphorylase (manC), with both PMI (E.C. 5.3.1.8) and GMP (E.C.2.7.7.13) activities

694521

Phosphomannose isomerase

Saccharomyces cerevisiae

-

-

702663

Phosphomannose isomerase

Sphingobium chungbukense

-

-

702869

Phosphomannose isomerase

Sphingobium chungbukense DJ77

-

-

-

phosphomannose-isomerase

Brassica napus

-

-

706204

phosphomannose-isomerase

Onchidium sp., Oncidium Gower Ramsey

-

-

716507

Phosphphexomutase

-

-

-

-

PMI

-

-

-

-

PMI

Alcaligenes sp.

-

-

701879

PMI

Alcaligenes sp. CGMCC2428

-

-

-

PMI

Brassica napus

-

-

706204

PMI

Escherichia coli

-

-

727158, 728469

PMI

Leishmania mexicana

Q9GRS9

-

652215

PMI

Onchidium sp., Oncidium Gower Ramsey

-

-

716507

PMI

Photorhabdus luminescens

-

-

713855

PMI

Photorhabdus luminescens TT01

-

-

-

PMI

Sphingobium chungbukense

-

-

702869

PMI

Sphingobium chungbukense DJ77

-

-

-

PMI/GMP

Sphingobium chungbukense

-

-

702869

PMI/GMP

Sphingobium chungbukense DJ77

-

-

-

Pmi1

Aspergillus fumigatus

Q66WM4

-

705568

Pmi1

Aspergillus fumigatus YJ-407

Q66WM4

-

-

PMI2

Brassica rapa subsp. chinensis

G1E7A7

-

728283

PslB

Pseudomonas aeruginosa

-

-

692361

PslB

Pseudomonas aeruginosa PAO1.

-

-

-

type I phosphomannose isomerase

Escherichia coli

-

-

716883

type I phosphomannose isomerases

Homo sapiens

-

-

716883

type I PMI

Escherichia coli, Homo sapiens

-

-

716883

9023-88-5

-

Aeropyrum pernix

biunctional phosphoglucose/phosphomannose isomerase EC 5.3.1.9 and 5.3.1.8, resp.

662220

Q9YE01

Swissprot

Alcaligenes sp.

strain CGMCC2428

701879

-

-

Alcaligenes sp. CGMCC2428

strain CGMCC2428

701879

-

-

Amorphophallus konjac

-

2809, 2810

-

-

Apis mellifera

-

2624

-

-

Arabidopsis thaliana

isoform Pmi1; isoform Pmi2

693149

-

-

Arabidopsis thaliana

syncytia induced by nematode Heterodera schachtii

700488

Q9M884

UniProt

Ascaris suum

-

2624

-

-

Aspergillus fumigatus

strain YJ-407, gene pmi1

705568

Q66WM4

UniProt

Aspergillus fumigatus YJ-407

strain YJ-407, gene pmi1

705568

Q66WM4

UniProt

Bacillus subtilis

enzyme from Bacillus subtilis strain HB002; ATCC 23857

701797

Q9AGZ4

UniProt

Bos taurus

-

2624

-

-

Brassica napus

different cultivars, gene pmi

706204

-

-

Brassica rapa subsp. chinensis

cultivar Suzhouqing

728283

G1E7A7

UniProt

Burkholderia cenocepacia

strain J2315, bifunctional enzyme with phosphomannose isomerase and guanosine diphosphate-D-mannose diphosphorylase activities

690577

-

-

Burkholderia cepacia

type II enzyme, bifunctional protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities

671785

Q06XM8

SwissProt

Candida albicans

-

2817, 2818, 2820, 2822, 2828

-

-

Candida albicans

wild-type and selenomethionine-labelled enzyme expressed in Escherichia coli

2825

-

-

Cassia coluteoides

-

2813

-

-

Cavia porcellus

-

2624

-

-

Cyamopsis tetragonoloba

-

2813

-

-

Enterobacter aerogenes

-

2624

-

-

Escherichia coli

-

2818, 2829, 3354, 660706, 661902, 663077, 663078, 663079, 663166, 702663, 716883, 727158, 728469

-

-

Escherichia coli

expression in Brassica rapa

681607, 682359

-

-

Escherichia coli

expression in Linum usitatissimum

682360

-

-

Escherichia coli

expression in Malus domestica

682353

-

-

Escherichia coli

expression in onion

682354

-

-

Geobacillus thermodenitrificans

-

701812, 702817

-

-

Geobacillus thermodenitrificans

-

726723

A4ITT1

UniProt

Glycine max

-

2813

-

-

Homo sapiens

-

2624, 2817, 2821, 2826, 2828, 681498, 716883

-

-

Leishmania mexicana

-

652215

Q9GRS9

SwissProt

Mammalia

-

2624

-

-

Microorganisms

-

2624

-

-

Mus musculus

-

662458

-

-

Nakazawaea holstii

-

2624

-

-

Onchidium sp.

-

716507

-

-

Oncidium Gower Ramsey

-

716507

-

-

Oryctolagus cuniculus

-

2624

-

-

Ovis aries

-

2624

-

-

Photorhabdus luminescens

-

713855

-

-

Photorhabdus luminescens TT01

-

713855

-

-

Placopecten magellanicus

-

705394

-

-

Pseudomonas aeruginosa

-

2624, 2814, 3354, 661119

-

-

Pseudomonas aeruginosa

bifunctional enzyme: phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase

2823, 2824

-

-

Pseudomonas aeruginosa

strain PAO1. Bifunctional enzyme with phosphomannose isomerase and guanosine diphosphate-D-mannose diphosphorylase activities

692361

-

-

Pseudomonas aeruginosa PAO1.

strain PAO1. Bifunctional enzyme with phosphomannose isomerase and guanosine diphosphate-D-mannose diphosphorylase activities

692361

-

-

Pyrobaculum aerophilum

bifunctional phosphoglucose isomerase/phosphomannose isomerase, EC 5.3.1.9 and 5.3.1.8, resp.

662267

Q8ZWV0

Uniprot

Pyrobaculum aerophilum

bifunctional phosphoglucose/phosphomannose isomerase EC 5.3.1.8 and EC 5.3.1.9, overexpression in Escherichia coli

661679

-

-

Pyrobaculum aerophilum

biunctional phosphoglucose/phosphomannose isomerase EC 5.3.1.9 and 5.3.1.8, resp.

660585

-

-

Pyrobaculum aerophilum

biunctional phosphoglucose/phosphomannose isomerase EC 5.3.1.9 and 5.3.1.8, resp.

661100

Q8ZWV0

Uniprot

Pyrococcus furiosus

DSM 3638

694521

-

-

Rattus norvegicus

-

2624, 2812

-

-

Saccharomyces cerevisiae

-

2624, 2806, 2807, 2811, 2816, 2817, 2828, 652637, 661119, 662318, 702663

-

-

Saccharomyces cerevisiae

secretory mutant with a thermolabile phosphomannose isomerase

2819

-

-

Salmonella enterica subsp. enterica serovar Typhimurium

-

690273

-

-

Salmonella enterica subsp. enterica serovar Typhimurium

-

701472

P25081

UniProt

Sphingobium chungbukense

strain DJ77, gene pmi

702869

-

-

Sphingobium chungbukense DJ77

strain DJ77, gene pmi

702869

-

-

Streptomyces nodosus

-

693999

-

-

Sulfolobus solfataricus

strain P1 (ATCC 35091)

680450

-

-

Sulfolobus solfataricus P1

strain P1 (ATCC 35091)

680450

-

-

Sus scrofa

-

2624, 2817, 2828

-

-

Thermoplasma acidophilum

biunctional phosphoglucose/phosphomannose isomerase EC 5.3.1.9 and 5.3.1.8, resp.

662220

Q9HIC2

Swissprot

Thermus thermophilus

-

713854

-

-

Thermus thermophilus

-

714372

Q5SIM4

UniProt

Thermus thermophilus KCCM 40879

-

713854

-

-

Thermus thermophilus KCCM 40879

-

714372

Q5SIM4

UniProt

Trigonella foenum-graecum

-

2813

-

-

Vigna angularis

-

693863

-

-

Xanthomonas campestris

-

2827

-

-

malfunction

Aspergillus fumigatus

-, Q66WM4

uncoupling of the link between energy production and glycosylation by deletion of the pmi1 gene leads to phenotypes such as defects in cell wall integrity, abnormal morphology and reduced conidiation, overview

705568

malfunction

Photorhabdus luminescens

-

the manA-deficient mutant is unable to utilize D-mannose as a sole carbon source

713855

malfunction

Aspergillus fumigatus YJ-407

-

uncoupling of the link between energy production and glycosylation by deletion of the pmi1 gene leads to phenotypes such as defects in cell wall integrity, abnormal morphology and reduced conidiation, overview

-

malfunction

Photorhabdus luminescens TT01

-

the manA-deficient mutant is unable to utilize D-mannose as a sole carbon source

-

metabolism

Alcaligenes sp.

-

the enzyme is part of the biosynthetic pathway of sugar nucleotides essential for welan gum production in Alcaligenes sp., overview

701879

metabolism

Sphingobium chungbukense

-

phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate in the extracellular polysaccharide synthesis pathway, feedback regulation of this pathway

702869

metabolism

Aspergillus fumigatus

-, Q66WM4

the enzyme catalyzes the first committed step in the synthesis of mannose-containing sugar chains and provides a link between glucose metabolism and mannosylation

705568

metabolism

Alcaligenes sp. CGMCC2428

-

the enzyme is part of the biosynthetic pathway of sugar nucleotides essential for welan gum production in Alcaligenes sp., overview

-

metabolism

Aspergillus fumigatus YJ-407

-

the enzyme catalyzes the first committed step in the synthesis of mannose-containing sugar chains and provides a link between glucose metabolism and mannosylation

-

metabolism

Sphingobium chungbukense DJ77

-

phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate in the extracellular polysaccharide synthesis pathway, feedback regulation of this pathway

-

physiological function

Photorhabdus luminescens

-

phosphomannose isomerase has a major effect on the formation of a complete, branched extracellular polysaccharide. The manA gene influences biofilm maturation but not initial attachment

713855

physiological function

Brassica rapa subsp. chinensis

G1E7A7

isoform PMI2 overexpression in tobacco can increase tolerance to oxidative stress

728283

physiological function

Photorhabdus luminescens TT01

-

phosphomannose isomerase has a major effect on the formation of a complete, branched extracellular polysaccharide. The manA gene influences biofilm maturation but not initial attachment

-

alpha-D-fructose 6-phosphate

alpha-D-mannose 6-phosphate

Pyrococcus furiosus

-

-

694521

-

-

r

beta-D-mannose 6-phosphate

beta-D-fructose 6-phosphate

Escherichia coli, Homo sapiens

-

-

716883

-

-

r

D-Allose

D-Psicose

Thermus thermophilus

-

-

713854

-

-

r

D-Allose

D-Psicose

Bacillus subtilis

-, Q9AGZ4

-

701797

-

-

r

D-Allose

D-Psicose

Thermus thermophilus KCCM 40879

-

-

713854

-

-

r

D-fructose 6-phosphate

D-glucose 6-phosphate

Aeropyrum pernix

Q9YE01

-

662220

-

-

r

D-fructose 6-phosphate

D-glucose 6-phosphate

Thermoplasma acidophilum

Q9HIC2

-

662220

-

-

r

D-fructose 6-phosphate

D-mannose 6-phosphate

Escherichia coli

-

-

661902

-

-

r

D-fructose 6-phosphate

D-mannose 6-phosphate

Pyrobaculum aerophilum

-

-

661679

-

-

r

D-fructose 6-phosphate

D-mannose 6-phosphate

Leishmania mexicana

-, Q9GRS9

-

652215

-

652215

r

D-fructose 6-phosphate

D-mannose 6-phosphate

Aeropyrum pernix

Q9YE01

-

662220

-

-

r

D-fructose 6-phosphate

D-mannose 6-phosphate

Thermoplasma acidophilum

Q9HIC2

-

662220

-

-

r

D-fructose 6-phosphate

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

-

714372

-

-

r

D-fructose 6-phosphate

D-mannose 6-phosphate

Leishmania mexicana

-, Q9GRS9

first step in the biosynthesis of activated mannose donors required for the biosynthesis of various glycoconjugates

652215

-

652215

r

D-fructose 6-phosphate

D-mannose 6-phosphate

Thermus thermophilus KCCM 40879

Q5SIM4

-

714372

-

-

r

D-Fructose 6-phosphate

?

Homo sapiens

-

-

2624

-

-

-

D-Fructose 6-phosphate

?

Homo sapiens

-

first enzyme of the N-glycosylation pathway

2821

-

-

-

D-Fructose 6-phosphate

?

Microorganisms

-

mannose 6-phosphate produced in this manner can be incorporated via mannose 1-phosphate as intermediate into guanosine diphosphomannose which participates in numerous processes related to cell wall biosynthesis

2624

-

-

-

D-Fructose 6-phosphate

?

Escherichia coli

-

enzyme is involved in alginate biosynthesis

3354

-

-

-

D-glucose 6-phosphate

D-fructose 6-phosphate

Sulfolobus solfataricus

-

-

680450

-

-

?

D-glucose 6-phosphate

D-fructose 6-phosphate

Aeropyrum pernix

Q9YE01

-

662220

-

-

r

D-glucose 6-phosphate

D-fructose 6-phosphate

Thermoplasma acidophilum

Q9HIC2

-

662220

-

-

r

D-glucose 6-phosphate

D-fructose 6-phosphate

Sulfolobus solfataricus P1

-

-

680450

-

-

?

D-Lyxose

D-Xylulose

Thermus thermophilus

-

-

713854

-

-

r

D-Lyxose

D-Xylulose

Bacillus subtilis

-, Q9AGZ4

-

701797

-

-

r

D-Lyxose

D-Xylulose

Geobacillus thermodenitrificans

-

38% conversion yield after 3 h

702817

-

-

?

D-Lyxose

D-Xylulose

Thermus thermophilus KCCM 40879

-

-

713854

-

-

r

D-Mannose

D-Fructose

Thermus thermophilus

-

-

713854

-

-

r

D-Mannose

D-Fructose

Bacillus subtilis

-, Q9AGZ4

-

701797

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Cavia porcellus, Mammalia

-

-

2624

-

2624

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Escherichia coli

-

-

2818

-

2818

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Escherichia coli

-

-

2829

-

2829

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Escherichia coli

-

-

3354

-

3354

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Escherichia coli

-

-

727158, 728469

-

-

?

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Escherichia coli

-

-

661902, 702663

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Homo sapiens

-

-

2624

-

2624

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Homo sapiens

-

-

2817

-

2817

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Homo sapiens

-

-

2821

-

2821

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Homo sapiens

-

-

2826

-

2826

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Homo sapiens

-

-

2828

-

2828

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Rattus norvegicus

-

-

2624

-

2624

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Rattus norvegicus

-

-

2812

-

2812

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Sus scrofa

-

-

2624

-

2624

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Sus scrofa

-

-

2817

-

2817

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Sus scrofa

-

-

2828

-

2828

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Saccharomyces cerevisiae

-

-

2624

-

2624

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Saccharomyces cerevisiae

-

-

2806

-

2806

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Saccharomyces cerevisiae

-

-

2816

-

2816

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Saccharomyces cerevisiae

-

-

2817

-

2817

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Saccharomyces cerevisiae

-

-

2819

-

2819

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Saccharomyces cerevisiae

-

-

2828

-

2828

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Saccharomyces cerevisiae

-

-

661119

-

-

?

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Saccharomyces cerevisiae

-

-

702663

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Bos taurus, Oryctolagus cuniculus, Ovis aries

-

-

2624

-

2624

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Glycine max

-

-

2813

-

2813

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Arabidopsis thaliana

-

-

693149

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Enterobacter aerogenes, Pseudomonas aeruginosa

-

-

2624

-

2624

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Pseudomonas aeruginosa

-

-

2814

-

2814

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Pseudomonas aeruginosa

-

-

2823

-

2823

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Pseudomonas aeruginosa

-

-

2824

-

2824

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Pseudomonas aeruginosa

-

-

3354

-

3354

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Pseudomonas aeruginosa

-

-

661119

-

-

?

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Pseudomonas aeruginosa

-

-

692361

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Brassica napus

-

-

706204

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Ascaris suum, Apis mellifera

-

-

2624

-

2624

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Candida albicans

-

-

2817

-

2817

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Candida albicans

-

-

2818

-

2818

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Candida albicans

-

-

2820

-

2820

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Candida albicans

-

-

2822

-

2822

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Candida albicans

-

-

2825

-

2825

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Candida albicans

-

-

2828

-

2828

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Alcaligenes sp.

-

-

701879

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Xanthomonas campestris

-

-

2827

-

2827

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Nakazawaea holstii, Microorganisms

-

-

2624

-

2624

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Amorphophallus konjac

-

-

2809

-

2809

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Amorphophallus konjac

-

-

2810

-

2810

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Cassia coluteoides, Cyamopsis tetragonoloba, Trigonella foenum-graecum

-

-

2813

-

2813

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Sulfolobus solfataricus

-

-

680450

-

-

?

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Pyrobaculum aerophilum

-

-

661679

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Geobacillus thermodenitrificans

-

-

701812, 702817

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Aeropyrum pernix

Q9YE01

-

662220

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Thermoplasma acidophilum

Q9HIC2

-

662220

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Burkholderia cenocepacia

-

-

690577

-

-

?

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Burkholderia cepacia

-, Q06XM8

-

671785

-

-

?

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Sphingobium chungbukense

-

-

702869

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Salmonella enterica subsp. enterica serovar Typhimurium

P25081

-

701472

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Bacillus subtilis

-, Q9AGZ4

-

701797

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Aspergillus fumigatus

-, Q66WM4

-

705568

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Geobacillus thermodenitrificans

A4ITT1, -

-

726723

-

-

?

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Brassica rapa subsp. chinensis

G1E7A7

-

728283

-

-

?

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Saccharomyces cerevisiae

-

beta-D-fructose 6-phosphate serves as substrate in the reverse direction

2811

-

2811

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Saccharomyces cerevisiae

-

highly specific for the beta anomer of mannose 6-phosphate, the alpha anomer has no activity as a substrate, and is, at best, a poor inhibitor

2807

-

2807

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Thermus thermophilus

Q5SIM4

best substrate

714372

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Pseudomonas aeruginosa PAO1.

-

-

692361

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Burkholderia cepacia IST408

-

-

671785

-

-

?

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Thermus thermophilus KCCM 40879

Q5SIM4

best substrate

714372

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Sphingobium chungbukense DJ77

-

-

702869

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Sulfolobus solfataricus P1

-

-

680450

-

-

?

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Aspergillus fumigatus YJ-407

Q66WM4

-

705568

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Alcaligenes sp. CGMCC2428

-

-

701879

-

-

r

D-Mannose 6-phosphate

?

Cassia coluteoides

-

enzyme is involved in the utilization of mannose released by hydrolysis of galactomannan on germination after phosphorylation to mannose 6-phosphate

2813

-

-

-

D-Mannose 6-phosphate

?

Mammalia

-

channeling D-mannose 6-phosphate into glycolysis by isomerization to fructose 6-phosphate

2624

-

-

-

D-Ribose

D-Ribulose

Thermus thermophilus

-

-

713854

-

-

r

D-Ribose

D-Ribulose

Bacillus subtilis

-, Q9AGZ4

-

701797

-

-

r

D-Ribose

D-Ribulose

Thermus thermophilus KCCM 40879

-

-

713854

-

-

r

D-ribose 5-phosphate

D-ribulose 5-phosphate

Thermus thermophilus, Thermus thermophilus KCCM 40879

Q5SIM4

very low activity

714372

-

-

r

D-talose

D-tagatose

Thermus thermophilus

-

-

713854

-

-

r

D-talose

D-tagatose

Bacillus subtilis

-, Q9AGZ4

-

701797

-

-

r

L-allose

L-psicose

Thermus thermophilus

-

-

713854

-

-

r

L-allose

L-psicose

Bacillus subtilis

-, Q9AGZ4

-

701797

-

-

r

L-Lyxose

L-Xylulose

Thermus thermophilus

-

-

713854

-

-

r

L-Lyxose

L-Xylulose

Bacillus subtilis

-, Q9AGZ4

-

701797

-

-

r

L-Lyxose

L-Xylulose

Thermus thermophilus KCCM 40879

-

-

713854

-

-

r

L-Mannose

L-Fructose

Thermus thermophilus

-

-

713854

-

-

r

L-Mannose

L-Fructose

Bacillus subtilis

-, Q9AGZ4

-

701797

-

-

r

L-Mannose

L-Fructose

Thermus thermophilus KCCM 40879

-

-

713854

-

-

r

L-ribose

L-ribulose

Thermus thermophilus

-

-

713854

-

-

r

L-ribose

L-ribulose

Bacillus subtilis

-, Q9AGZ4

-

701797

-

-

r

L-ribose

L-ribulose

Geobacillus thermodenitrificans

-

29% conversion yield after 3 h

702817

-

-

?

L-ribulose

L-ribose

Thermus thermophilus

-

highest activity

713854

-

-

r

L-ribulose

L-ribose

Bacillus subtilis

-, Q9AGZ4

best substrate

701797

-

-

r

L-talose

L-tagatose

Thermus thermophilus

-

-

713854

-

-

r

L-talose

L-tagatose

Bacillus subtilis

-, Q9AGZ4

-

701797

-

-

r

additional information

?

-

Homo sapiens

-

-

2624

-

-

-

additional information

?

-

Enterobacter aerogenes

-

constitutive enzyme

2624

-

-

-

additional information

?

-

Candida albicans

-

enzyme is involved in biosynthesis of mannan components of the cell walls

2818, 2820

-

-

-

additional information

?

-

Pseudomonas aeruginosa

-

adaptive enzyme

2624

-

-

-

additional information

?

-

Homo sapiens

-

critical role in the supply of the D-mannose derivatives required for many glycosylation reactions

2826

-

-

-

additional information

?

-

Candida albicans

-

reversible isomerization of mannose 6-phosphate and fructose 6-phosphate is the initial commited step in the synthesis of the mannosylated glycoproteins, which are essential in biosynthesis of functional fungal cell wall. Absence of mannose-6-phosphate isomerase causes cell lysis

2822

-

-

-

additional information

?

-

Sulfolobus solfataricus

-

no catalytic activity toward glucosamine 6-phosphate or N-acetylglucosamine 6-phosphate

680450

-

-

-

additional information

?

-

Geobacillus thermodenitrificans

-

enzyme substrate specificity with diverse monosaccharide aldoses and ketoses, overview

702817

-

-

-

additional information

?

-

Escherichia coli, Saccharomyces cerevisiae

-

Molecular mechanics study of enzyme substrate and inhibitors, overview

702663

-

-

-

additional information

?

-

Sphingobium chungbukense

-

PMI is bifunctional possessing both mannose 6-phosphate isomerase and GDP-mannose diphosphorylase activities

702869

-

-

-

additional information

?

-

Bacillus subtilis

-, Q9AGZ4

the enzyme is specific for aldose substrates possessing hydroxyl groups oriented in the same direction at the C-2 and C-3 positions, such as the D- and L-enantiomers of ribose, lyxose, talose, mannose, and allose, substrate specificity, overview

701797

-

-

-

additional information

?

-

Thermus thermophilus, Thermus thermophilus KCCM 40879

Q5SIM4

no activity with D-glucose 6-phosphate and arabinose 5-phosphate

714372

-

-

-

additional information

?

-

Sphingobium chungbukense DJ77

-

PMI is bifunctional possessing both mannose 6-phosphate isomerase and GDP-mannose diphosphorylase activities

702869

-

-

-

additional information

?

-

Sulfolobus solfataricus P1

-

no catalytic activity toward glucosamine 6-phosphate or N-acetylglucosamine 6-phosphate

680450

-

-

-

D-fructose 6-phosphate

D-mannose 6-phosphate

Leishmania mexicana

-, Q9GRS9

first step in the biosynthesis of activated mannose donors required for the biosynthesis of various glycoconjugates

652215

-

652215

r

D-Fructose 6-phosphate

?

Homo sapiens

-

-

2624

-

-

-

D-Fructose 6-phosphate

?

Homo sapiens

-

first enzyme of the N-glycosylation pathway

2821

-

-

-

D-Fructose 6-phosphate

?

Microorganisms

-

mannose 6-phosphate produced in this manner can be incorporated via mannose 1-phosphate as intermediate into guanosine diphosphomannose which participates in numerous processes related to cell wall biosynthesis

2624

-

-

-

D-Fructose 6-phosphate

?

Escherichia coli

-

enzyme is involved in alginate biosynthesis

3354

-

-

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Escherichia coli

-

-

727158, 728469

-

-

?

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Escherichia coli, Saccharomyces cerevisiae

-

-

702663

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Brassica napus

-

-

706204

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Alcaligenes sp.

-

-

701879

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Geobacillus thermodenitrificans

-

-

701812, 702817

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Sphingobium chungbukense

-

-

702869

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Salmonella enterica subsp. enterica serovar Typhimurium

P25081

-

701472

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Bacillus subtilis

-, Q9AGZ4

-

701797

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Aspergillus fumigatus

-, Q66WM4

-

705568

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Geobacillus thermodenitrificans

A4ITT1, -

-

726723

-

-

?

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Brassica rapa subsp. chinensis

G1E7A7

-

728283

-

-

?

D-Mannose 6-phosphate

?

Cassia coluteoides

-

enzyme is involved in the utilization of mannose released by hydrolysis of galactomannan on germination after phosphorylation to mannose 6-phosphate

2813

-

-

-

D-Mannose 6-phosphate

?

Mammalia

-

channeling D-mannose 6-phosphate into glycolysis by isomerization to fructose 6-phosphate

2624

-

-

-

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Sphingobium chungbukense DJ77

-

-

702869

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Aspergillus fumigatus YJ-407

Q66WM4

-

705568

-

-

r

D-Mannose 6-phosphate

D-Fructose 6-phosphate

Alcaligenes sp. CGMCC2428

-

-

701879

-

-

r

additional information

?

-

Homo sapiens

-

-

2624

-

-

-

additional information

?

-

Enterobacter aerogenes

-

constitutive enzyme

2624

-

-

-

additional information

?

-

Candida albicans

-

enzyme is involved in biosynthesis of mannan components of the cell walls

2818, 2820

-

-

-

additional information

?

-

Pseudomonas aeruginosa

-

adaptive enzyme

2624

-

-

-

additional information

?

-

Homo sapiens

-

critical role in the supply of the D-mannose derivatives required for many glycosylation reactions

2826

-

-

-

additional information

?

-

Candida albicans

-

reversible isomerization of mannose 6-phosphate and fructose 6-phosphate is the initial commited step in the synthesis of the mannosylated glycoproteins, which are essential in biosynthesis of functional fungal cell wall. Absence of mannose-6-phosphate isomerase causes cell lysis

2822

-

-

-

Ba2+

Bacillus subtilis

-, Q9AGZ4

activates

701797

Ca2+

Pseudomonas aeruginosa

-

activates

2824

Ca2+

Xanthomonas campestris

-

-

2827

Ca2+

Burkholderia cepacia

-, Q06XM8

best activator, may be replaced by Mg2+ or Mn2+ for phosphomannose isomerase activity

671785

Ca2+

Salmonella enterica subsp. enterica serovar Typhimurium

P25081

activates

701472

Ca2+

Bacillus subtilis

-, Q9AGZ4

activates

701797

Ca2+

Geobacillus thermodenitrificans

-

activates

702817

Ca2+

Sphingobium chungbukense

-

activates

702869

Ca2+

Thermus thermophilus

Q5SIM4

the enzyme is not stimulated by Ca2+

714372

Co2+

Saccharomyces cerevisiae

-

can reverse inhibition by EDTA

2806

Co2+

Amorphophallus konjac

-

can reverse inhibition by a metal binding agent

2809

Co2+

Pseudomonas aeruginosa

-

best activator

2824

Co2+

Xanthomonas campestris

-

activates

2827

Co2+

Pseudomonas aeruginosa

-

required for phosphomannose isomerase activity

692361

Co2+

Salmonella enterica subsp. enterica serovar Typhimurium

P25081

activates

701472

Co2+

Bacillus subtilis

-, Q9AGZ4

activates, best metal ion, maximal activity at 0.5 mM

701797

Co2+

Geobacillus thermodenitrificans

-

-

701812

Co2+

Geobacillus thermodenitrificans

-

activates, best activator metal ion

702817

Co2+

Sphingobium chungbukense

-

beste metal ion activator

702869

Co2+

Thermus thermophilus

-

about 230% activity at 0.5 mM

713854, 714372

Co2+

Geobacillus thermodenitrificans

A4ITT1, -

strong stimulation of activity

726723

Cu2+

Bacillus subtilis

-, Q9AGZ4

activates

701797

Cu2+

Geobacillus thermodenitrificans

-

activates

702817

Cu2+

Thermus thermophilus

-

maximal activity of the recombinant enzyme for L-ribulose isomerization in the presence of 0.5 mM Cu2+

713854

Cu2+

Thermus thermophilus

Q5SIM4

about 135% activity at 0.5 mM

714372

Cu2+

Geobacillus thermodenitrificans

A4ITT1, -

significantly stimulated by Cu2+

726723

Fe2+

Saccharomyces cerevisiae

-

can reverse inhibition by EDTA

2806

Fe2+

Amorphophallus konjac

-

can reverse inhibition by a metal binding agent

2809

Fe2+

Geobacillus thermodenitrificans

-

activates

702817

Fe2+

Thermus thermophilus

Q5SIM4

about 125% activity at 0.5 mM

714372

Mg2+

Pseudomonas aeruginosa

-

activates

2824

Mg2+

Burkholderia cepacia

-, Q06XM8

60% of activity with Ca2+ for phosphomannose isomerase activity

671785

Mg2+

Salmonella enterica subsp. enterica serovar Typhimurium

P25081

best activating divalent metal ion

701472

Mg2+

Bacillus subtilis

-, Q9AGZ4

activates

701797

Mg2+

Geobacillus thermodenitrificans

-

activates

702817

Mg2+

Sphingobium chungbukense

-

activates only the GMP activity of the enzyme

702869

Mg2+

Thermus thermophilus

Q5SIM4

about 175% activity at 0.5 mM

714372

Mn2+

Amorphophallus konjac

-

can reverse inhibition by a metal binding agent

2809

Mn2+

Pseudomonas aeruginosa

-

activates

2824

Mn2+

Xanthomonas campestris

-

activates

2827

Mn2+

Burkholderia cepacia

-, Q06XM8

65% of activity with Ca2+ for phosphomannose isomerase activity

671785

Mn2+

Salmonella enterica subsp. enterica serovar Typhimurium

P25081

activates

701472

Mn2+

Bacillus subtilis

-, Q9AGZ4

highly activating

701797

Mn2+

Geobacillus thermodenitrificans

-

activates

702817

Mn2+

Thermus thermophilus

-

about 160% activity at 0.5 mM

713854

Mn2+

Thermus thermophilus

Q5SIM4

about 175% activity at 0.5 mM

714372

Ni2+

Pseudomonas aeruginosa

-

activates

2824

Ni2+

Xanthomonas campestris

-

-

2827

Ni2+

Salmonella enterica subsp. enterica serovar Typhimurium

P25081

activates

701472

Ni2+

Bacillus subtilis

-, Q9AGZ4

activates

701797

Ni2+

Geobacillus thermodenitrificans

-

activates

702817

Ni2+

Sphingobium chungbukense

-

activates

702869

Zinc

Saccharomyces cerevisiae

-

contains 1 gatom per mol of enzyme

2806

Zinc

Candida albicans

-

enzyme contains an essential zinc atom. Four of the five ligands come from the protein, one of these ligands is unique in that it is a glutamine

2822

Zinc

Pseudomonas aeruginosa

-

weak activation

2824

Zinc

Candida albicans, Homo sapiens

-

recombinant enzyme expressed in E. coli contains slightly less than 1 mol of zinc per mol of proteins

2828

Zinc

Saccharomyces cerevisiae

-

-

2828

Zn2+

Saccharomyces cerevisiae

-

can reverse inhibition by EDTA

2806

Zn2+

Amorphophallus konjac

-

at low concentrations complete reactivation of enzyme inhibited by a metal binding agent

2809

Zn2+

Xanthomonas campestris

-

activates

2827

Zn2+

Escherichia coli

-

noncovalent complexation, ESI-FTICR study, binding/release of metal ion changes substrate binding affinity by at least 5fold

660706

Zn2+

Salmonella enterica subsp. enterica serovar Typhimurium

P25081

enzyme-bound, Zn2+ binding induces structural order in the loop consisting of residues 50-54. The metal atom appears to play a role in substrate binding and is probably also important for maintaining the architecture of the active site

701472

Zn2+

Bacillus subtilis

-, Q9AGZ4

activates

701797

Zn2+

Geobacillus thermodenitrificans

-

activates poorly

702817

Zn2+

Sphingobium chungbukense

-

activates

702869

Zn2+

Aspergillus fumigatus

-, Q66WM4

-

705568

Zn2+

Thermus thermophilus

-

about 130% activity at 0.5 mM

713854

Zn2+

Thermus thermophilus

Q5SIM4

Zn2+ is present at one molecule per monomer, the enzyme has about 240% activity in the presence of 0.5 mM Zn2+

714372

Zn2+

Escherichia coli, Homo sapiens

-

zinc-dependent metalloenzyme

716883

Mn2+

Geobacillus thermodenitrificans

A4ITT1, -

significantly stimulated by Mn2+

726723

additional information

Pseudomonas aeruginosa

-

activated by bivalent cations in the order: Co2+, Ni2+, Mn2+, Mg2+, Ca2+, Zn2+

2824

additional information

Xanthomonas campestris

-

activated by bivalent cations in decreasing order: Co2+, Zn2+, Mn2+, Ni2+, Ca2+

2827

additional information

Salmonella enterica subsp. enterica serovar Typhimurium

P25081

divalent cations are absolutely required for activity, metal binding increases the thermostability of the enzyme

701472

additional information

Bacillus subtilis

-, Q9AGZ4

mannose-6-phosphate isomerases are metalloenzymes that require a divalent ion metal cofactor for activity and catalysis

701797

additional information

Geobacillus thermodenitrificans

-

the enzyme requires divalent cations for activity

702817

additional information

Sphingobium chungbukense

-

PMI absolutely requires divalent metal cations for catalytic activity

702869

additional information

Thermus thermophilus

-

the enzyme is not affected by Ca2+, Mg2+, Ba2+, and Ni2+

713854

0.000041 {5-phospho-D-arabinonohydroxamic acid}

Homo sapiens

-

in 50 mM HEPES buffer, pH 7.1, at 25°C

716883

-

0.0006 {5-phospho-D-arabinonhydrazide}

Homo sapiens

-

in 50 mM HEPES buffer, pH 7.1, at 25°C

716883

-

1,10-phenanthroline

Amorphophallus konjac

-

-

2809, 2810

2D-image

1,10-phenanthroline

Candida albicans, Homo sapiens

-

-

2828

2D-image

1,10-phenanthroline

Saccharomyces cerevisiae

-

-

2806, 2828

2D-image

1,10-phenanthroline

Sus scrofa

-

-

2828

2D-image

2,2'-dipyridyl

Saccharomyces cerevisiae

-

-

2806

2D-image

2,2'-dipyridyl

Amorphophallus konjac

-

-

2810

2D-image

2,3-Butanedione

Pseudomonas aeruginosa

-

inactivation

692361

2D-image

5-deoxy-5-(dihydrogenophosphonomethyl)-D-arabinono-1,4-lactone

Escherichia coli, Saccharomyces cerevisiae

-

-

702663

2D-image

5-deoxy-5-malonyl-D-arabinonohydroxamic acid

Escherichia coli

-

-

727158

-

5-deoxy-5-phosphonomethyl-D-arabinonate

Escherichia coli, Saccharomyces cerevisiae

-

-

702663

2D-image

5-deoxy-5-phosphonomethyl-D-arabinonohydrazide

Escherichia coli, Saccharomyces cerevisiae

-

-

702663

2D-image

5-deoxy-5-phosphonomethyl-D-arabinonohydroxamic acid

Escherichia coli, Saccharomyces cerevisiae

-

-

702663

2D-image

5-phospho-D-arabinonhydrazide

Escherichia coli, Homo sapiens

-

-

716883

2D-image

5-phospho-D-arabinonohydroxamic acid

Pseudomonas aeruginosa

-

50% inhibition at 0.000169 mM, inhibition of both type I and type II isozymes

661119

2D-image

5-phospho-D-arabinonohydroxamic acid

Saccharomyces cerevisiae

-

50% inhibition at 0.000136 mM, inhibition of both type I and type II isozymes

661119

2D-image

5-phospho-D-arabinonohydroxamic acid

Escherichia coli, Homo sapiens

-

nanomolar inhibitor

716883

2D-image

5-phospho-D-arabinonohydroxamic acid

Escherichia coli

-

-

727158

2D-image

5-phosphoarabinonate

Pyrobaculum aerophilum

Q8ZWV0

-

662267

2D-image

6-deoxy-6-carboxymethyl-D-mannose

Escherichia coli, Saccharomyces cerevisiae

-

-

702663

2D-image

6-deoxy-6-dicarboxymethyl-D-mannose

Escherichia coli, Saccharomyces cerevisiae

-

-

702663

2D-image

6-deoxy-6-dimethylmalonate-D-mannopyranose

Escherichia coli, Saccharomyces cerevisiae

-

-

702663

2D-image

6-deoxy-6-phosphonomethyl-D-mannose

Escherichia coli, Saccharomyces cerevisiae

-

-

702663

2D-image

6-phospho-2-deoxygluconate

Cassia coluteoides

-

competitive

2813

2D-image

6-phosphogluconate

Saccharomyces cerevisiae

-

competitive

2624

2D-image

6-phosphogluconate

Cassia coluteoides

-

slight inhibition

2813

2D-image

6-phosphogluconate

Pyrobaculum aerophilum

-

-

661679

2D-image

6-phosphogluconate

Aeropyrum pernix

Q9YE01

-

662220

2D-image

6-phosphogluconate

Thermoplasma acidophilum

Q9HIC2

-

662220

2D-image

6-Phosphomannonate

Cassia coluteoides

-

competitive

2813

2D-image

8-hydroxyquinoline

Saccharomyces cerevisiae

-

-

2806

2D-image

ADP-glucose

Pseudomonas aeruginosa

-

0.5 mM, 77.8% of initial activity

692361

2D-image

Ag+

Candida albicans

-

irreversible inhibition in a two-step process, mannose 6-phosphate protects against inactivation. Mutant enzyme Cys150Ala shows 1000fold less sensitivity than the wild-type enzyme

2818

2D-image

alpha-D-Mannose 6-phosphate

Saccharomyces cerevisiae

-

poor

2807

2D-image

arabinose 5-phosphate

Candida albicans, Homo sapiens, Saccharomyces cerevisiae, Sus scrofa

-

-

2828

2D-image

Ba2+

Thermus thermophilus

Q5SIM4

about 30% inhibition at 0.5 mM

714372

2D-image

benzyl 2,3,4-tri-O-benzyl-6-deoxy-6-dimethylmalonate-alpha-D-mannopyranoside

Escherichia coli, Saccharomyces cerevisiae

-

-

702663

2D-image

benzyl 2,3,4-tri-O-benzyl-6-O-trifluoromethanesulfonyl-alpha-D-mannose

Escherichia coli, Saccharomyces cerevisiae

-

-

702663

2D-image

benzyl 2,3,4-tri-O-benzyl-alpha-D-mannose

Escherichia coli, Saccharomyces cerevisiae

-

-

702663

2D-image

Cd2+

Saccharomyces cerevisiae

-

competitive, strongly pH dependent

2816

2D-image

Cd2+

Candida albicans

-

-

2825

2D-image

Cd2+

Arabidopsis thaliana

-

1 mM, no residual activity

693149

2D-image

cysteine

Saccharomyces cerevisiae

-

-

2806

2D-image

D-mannose 1-phosphate

Pseudomonas aeruginosa

-

0.5 mM, 81.9% of initial activity

692361

2D-image

diethyldicarbonate

Xanthomonas campestris

-

-

2827

2D-image

dithiothreitol

Pseudomonas aeruginosa

-

-

2824

2D-image

dithiothreitol

Candida albicans, Homo sapiens

-

-

2828

2D-image

dithiothreitol

Saccharomyces cerevisiae

-

-

2806, 2828

2D-image

dithiothreitol

Sus scrofa

-

-

2828

2D-image

dithiothreitol

Arabidopsis thaliana

-

1 mM, 73% residual activity; 1 mM, 84% residual activity

693149

2D-image

EDTA

Amorphophallus konjac

-

-

2809, 2810

2D-image

EDTA

Candida albicans, Homo sapiens

-

-

2828

2D-image

EDTA

Saccharomyces cerevisiae

-

-

2806, 2828

2D-image

EDTA

Sus scrofa

-

-

2828

2D-image

EDTA

Burkholderia cepacia

-, Q06XM8

2 mM, complete inhibition

671785

2D-image

EDTA

Arabidopsis thaliana

-

2.5 mM, 54% residual activity; 2.5 mM, 86% residual activity

693149

2D-image

EDTA

Salmonella enterica subsp. enterica serovar Typhimurium

P25081

complete inhibition at 1 mM, reversible by the addition of divalent metal cations such as Zn2+, Mn2+, Co2+, Mg2+ and Ni2+

701472

2D-image

EDTA

Bacillus subtilis

-, Q9AGZ4

complete inactivation

701797

2D-image

EDTA

Geobacillus thermodenitrificans

-

strong inhibition

702817

2D-image

EDTA

Thermus thermophilus

-

about 75% residual activity at 0.5 mM

713854

2D-image

EDTA

Thermus thermophilus

Q5SIM4

complete inhibition at 0.5 mM

714372

2D-image

EDTA

Geobacillus thermodenitrificans

A4ITT1, -

-

726723

2D-image

erythrose 4-phosphate

Saccharomyces cerevisiae

-

; competitive

2624

2D-image

erythrose 4-phosphate

Cassia coluteoides

-

D-erythrose 4-phosphate, competitive

2813

2D-image

erythrose 4-phosphate

Candida albicans

-

-

2828

2D-image

erythrose 4-phosphate

Homo sapiens

-

-

2624, 2828

2D-image

erythrose 4-phosphate

Saccharomyces cerevisiae

-

-

2828

2D-image

erythrose 4-phosphate

Sus scrofa

-

-

2624, 2828

2D-image

erythrose 4-phosphate

Escherichia coli

-

-

660706

2D-image

erythrose 4-phosphate

Pyrobaculum aerophilum

-

-

661679

2D-image

erythrose 4-phosphate

Aeropyrum pernix

Q9YE01

-

662220

2D-image

erythrose 4-phosphate

Thermoplasma acidophilum

Q9HIC2

-

662220

2D-image

Fe2+

Thermus thermophilus

-

12% residual activity at 0.5 mM

713854

2D-image

fructose 1-phosphate

Homo sapiens

-

competitive

2821

2D-image

Galactose 6-hosphate

Saccharomyces cerevisiae

-

competitive

2624

-

GDP-D-mannose

Sphingobium chungbukense

-

inhibits mannose 6-phosphate isomerase activity of PMI, feedback regulation of this pathway

702869

2D-image

GDP-mannose

Pseudomonas aeruginosa

-

0.5 mM, 46.7% of initial activity

692361

2D-image

glucosamine 6-phosphate

Saccharomyces cerevisiae

-

competitive

2624

2D-image

glucose 1-phosphate

Saccharomyces cerevisiae

-

competitive

2624

2D-image

glucose 6-phosphate

Saccharomyces cerevisiae

-

competitive

2624

2D-image

Hg2+

Saccharomyces cerevisiae

-

competitive, relatively pH-independent. Zn2+ and Hg2+ can simultaneously bind in the mannose 6-phosphate binding pocket, with only a small mutual repulsion

2816

2D-image

Hg2+

Candida albicans

-

partial noncompetitive inhibition with mannose 6-phosphate as substrate. In addition to the inhibition at rapid equilibrium, inactivation occurs in a two-step process, proceeding via an intermediate complex. The rate of the irreversible inactivation can be slowed by the addition of the substrate mannose 6-phosphate

2817

2D-image

Hg2+

Saccharomyces cerevisiae

-

-

2817

2D-image

Hg2+

Candida albicans

-

recombinant wild-type enzyme and selenomethionine-labelled enzyme

2825

2D-image

HgCl2

Amorphophallus konjac

-

-

2809

2D-image

KI-VALUE

Homo sapiens

-

-

716883

-

L-ascorbic acid

Arabidopsis thaliana

-

1 mM, 77% residual activity

693149

2D-image

mannitol 1-phosphate

Saccharomyces cerevisiae

-

competitive

2624

2D-image

mannitol 1-phosphate

Cassia coluteoides

-

competitive

2813

2D-image

N,2,3,4,5-pentahydroxypentanamide

Escherichia coli

-

-

727158

2D-image

p-chloromercuribenzoate

Arabidopsis thaliana

-

0.05 mM, 33% residual activity; 0.05 mM, 39% residual activity

693149

2D-image

PCMB

Amorphophallus konjac

-

-

2809

2D-image

phosphate

Saccharomyces cerevisiae

-

competitive

2624

2D-image

ribose 5-phosphate

Saccharomyces cerevisiae

-

competitive

2624

2D-image

S-nitroso-acetyl-penicillamine

Saccharomyces cerevisiae

-

dose- and time-dependent inactivation

652637

2D-image

Silver sulfadiazine

Candida albicans

-

wild-type enzyme is inhibited, mutant enzyme Cys150Ala is not inhibited

2818

2D-image

Silver sulfadiazine

Escherichia coli

-

no inhibition

2818

2D-image

sorbitol 6-phosphate

Saccharomyces cerevisiae

-

competitive

2624

2D-image

Y3+

Salmonella enterica subsp. enterica serovar Typhimurium

P25081

-

701472

2D-image

Zn2+

Saccharomyces cerevisiae

-

competitive with mannose 6-phosphate, strongly pH-dependent. Zn2+ and Hg2+ can simultaneously bind in the mannose 6-phosphate binding pocket, with only a small mutual repulsion

2816

2D-image

Zn2+

Candida albicans

-

-

2825

2D-image

Zn2+

Burkholderia cenocepacia

-

inhibitory to both phosphomannose isomerase and guanosine diphosphate-D-mannose diphosphorylase activities

690577

2D-image

Zn2+

Arabidopsis thaliana

-

1 mM, no residual activity

693149

2D-image

mannitol 1-phosphate

Escherichia coli

-

-

660706

2D-image

additional information

Saccharomyces cerevisiae

-

no inhibition by N-acetyl-penicillamine

652637

-

additional information

Pseudomonas aeruginosa, Saccharomyces cerevisiae

-

not inhibitory: 5-phospho-D-arabinonate

661119

-

additional information

Burkholderia cepacia

-, Q06XM8

not inhibitory: mannose 1-phosphate, GTP, GDP-mannose, or diphosphate

671785

-

additional information

Escherichia coli

-

synthesis of a non-hydrolyzable D-mannose 6-phosphate surrogates as strong competitive enzyme inhibitors. Effective binding to the catalytic site occurs with retention of the Zn(II)-bound water molecule. Molecular mechanics study of enzyme substrate and inhibitors, overview

702663

-

additional information

Saccharomyces cerevisiae

-

synthesis of a non-hydrolyzable D-mannose 6-phosphate surrogates as strong competitive inhibitors of the enzyme. Effective binding to the catalytic site occurs with retention of the Zn(II)-bound water molecule. Molecular mechanics study of enzyme substrate and inhibitors, overview

702663

-

additional information

Sphingobium chungbukense

-

no inhibition by GTP, mannose 1-phosphate, and phosphodiphosphate

702869

-

Ca2+

Burkholderia cenocepacia

-

order of activation Mg2+ > Ca2+ > Mn2+ > Co2+ > Ni2+

690577

2D-image

Co2+

Burkholderia cenocepacia

-

order of activation Mg2+ > Ca2+ > Mn2+ > Co2+ > Ni2+

690577

2D-image

diphosphate

Pseudomonas aeruginosa

-

0.5 mM, 120% of initial activity

692361

2D-image

Mg2+

Burkholderia cenocepacia

-

order of activation Mg2+ > Ca2+ > Mn2+ > Co2+ > Ni2+

690577

2D-image

Mn2+

Burkholderia cenocepacia

-

Mg2+ > Ca2+ > Mn2+ > Co2+ > Ni2+

690577

2D-image

Ni2+

Burkholderia cenocepacia

-

order of activation Mg2+ > Ca2+ > Mn2+ > Co2+ > Ni2+

690577

2D-image

0.00043

-

beta-D-mannose 6-phosphate

Homo sapiens

-

in 50 mM HEPES buffer, pH 7.1, at 25°C

716883

-

0.33

-

beta-D-mannose 6-phosphate

Escherichia coli

-

in 50 mM HEPES buffer, pH 7.1, at 25°C

716883

-

0.06

-

D-fructose 6-phosphate

Pyrobaculum aerophilum

-

pH 7.4, 80°C

661679

2D-image

0.15

-

D-fructose 6-phosphate

Escherichia coli

-

22°C, pH 7.4

661902

2D-image

0.2

-

D-fructose 6-phosphate

Thermoplasma acidophilum

Q9HIC2

80°C, pH 7.4

662220

2D-image

0.21

-

D-fructose 6-phosphate

Aeropyrum pernix

Q9YE01

50°C, pH 7.4

662220

2D-image

0.22

-

D-fructose 6-phosphate

Thermus thermophilus

Q5SIM4

wild type enzyme, pH 7.0, 80°C

714372

2D-image

0.3

-

D-fructose 6-phosphate

Pyrobaculum aerophilum

-

pH 7.4, 50°C

661679

2D-image

0.44

-

D-fructose 6-phosphate

Aeropyrum pernix

Q9YE01

80°C, pH 7.4

662220

2D-image

0.002

-

D-glucose 6-phosphate

Sulfolobus solfataricus

-

mutant enzyme S309D, at 65°C

680450

2D-image

0.006

-

D-glucose 6-phosphate

Sulfolobus solfataricus

-

mutant enzyme S309Q, at 65°C

680450

2D-image

0.008

-

D-glucose 6-phosphate

Sulfolobus solfataricus

-

mutant enzyme S309N, at 65°C; mutant enzyme S309T, at 65°C

680450

2D-image

0.009

-

D-glucose 6-phosphate

Sulfolobus solfataricus

-

mutant enzyme S309A, at 65°C

680450

2D-image

0.012

-

D-glucose 6-phosphate

Sulfolobus solfataricus

-

mutant enzyme S309V, at 65°C

680450

2D-image

0.013

-

D-glucose 6-phosphate

Sulfolobus solfataricus

-

wild type enzyme, at 65°C

680450

2D-image

0.72

-

D-glucose 6-phosphate

Thermoplasma acidophilum

Q9HIC2

80°C, pH 7.4

662220

2D-image

3.5

-

D-glucose 6-phosphate

Aeropyrum pernix

Q9YE01

80°C, pH 7.4

662220

2D-image

433

-

D-Lyxose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

2D-image

946

-

D-mannose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

2D-image

0.0413

-

D-mannose 6-phosphate

Arabidopsis thaliana

-

pH 7.5, 25°C

693149

2D-image

0.06

-

D-mannose 6-phosphate

Pyrobaculum aerophilum

-

pH 7.4, 80°C

661679

2D-image

0.121

-

D-mannose 6-phosphate

Saccharomyces cerevisiae

-

pH 7.1, 25°C

661119

2D-image

0.18

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme E132A, pH 7.0, 80°C; mutant enzyme E67A, pH 7.0, 80°C

714372

2D-image

0.21

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

wild type enzyme, pH 7.0, 80°C

714372

2D-image

0.25

-

D-mannose 6-phosphate

Thermoplasma acidophilum

Q9HIC2

80°C, pH 7.4

662220

2D-image

0.27

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme H122A, pH 7.0, 80°C

714372

2D-image

0.33

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme H50A, pH 7.0, 80°C

714372

2D-image

0.354

-

D-mannose 6-phosphate

Escherichia coli

-

in 50 mM HEPES buffer, pH 7.1, at 25°C

727158

2D-image

0.372

-

D-mannose 6-phosphate

Arabidopsis thaliana

-

pH 7.5, 25°C

693149

2D-image

0.53

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme Q48A, pH 7.0, 80°C

714372

2D-image

0.85

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme W13A, pH 7.0, 80°C

714372

2D-image

1.1

-

D-mannose 6-phosphate

Aeropyrum pernix

Q9YE01

80°C, pH 7.4

662220

2D-image

1.18

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

wild-type, pH 7.0, 25°C

692361

2D-image

1.21

-

D-mannose 6-phosphate

Escherichia coli

-

22°C, pH 7.4

661902

2D-image

1.29

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme R142A, pH 7.0, 80°C

714372

2D-image

2.5

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

pH 7.1, 25°C

661119

2D-image

8

9

D-mannose 6-phosphate

Geobacillus thermodenitrificans

A4ITT1, -

mutant enzyme N90A/L129F, at pH 7.0 and 70°C

726723

2D-image

9

-

D-mannose 6-phosphate

Burkholderia cepacia

-, Q06XM8

pH 7.6, presence of 5 mM Mg2+

671785

2D-image

9.04

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

mutant E410A, pH 7.0, 25°C

692361

2D-image

12.28

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

mutant R479A, pH 7.0, 25°C

692361

2D-image

12.36

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

mutant E458A, pH 7.0, 25°C

692361

2D-image

12.4

-

D-mannose 6-phosphate

Burkholderia cenocepacia

-

30°C, pH 7.6

690577

2D-image

12.9

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

mutant N433A, pH 7.0, 25°C

692361

2D-image

15.9

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

mutant R373A, pH 7.0, 25°C

692361

2D-image

16.26

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

mutant H411A, pH 7.0, 25°C

692361

2D-image

23.12

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

mutant R472A, pH 7.0, 25°C

692361

2D-image

100

-

D-mannose 6-phosphate

Geobacillus thermodenitrificans

A4ITT1, -

mutant enzyme W17Q/N90A/L129F, at pH 7.0 and 70°C

726723

2D-image

149

-

D-mannose 6-phosphate

Geobacillus thermodenitrificans

A4ITT1, -

wild type enzyme, at pH 7.0 and 70°C

726723

2D-image

243

-

D-mannose 6-phosphate

Geobacillus thermodenitrificans

A4ITT1, -

mutant enzyme L129F, at pH 7.0 and 70°C

726723

2D-image

110

-

D-ribose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

2D-image

469

-

D-talose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

2D-image

312

-

L-allose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

2D-image

998

-

L-ribose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

2D-image

136

-

L-ribulose

Thermus thermophilus

-

wild type enzyme, at pH 7.0 and 75°C

713854

2D-image

140

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142N, at pH 7.0 and 75°C

713854

2D-image

150

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142Q, at pH 7.0 and 75°C

713854

2D-image

151

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142E, at pH 7.0 and 75°C

713854

2D-image

184

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142A, at pH 7.0 and 75°C

713854

2D-image

228

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142K, at pH 7.0 and 75°C

713854

2D-image

308

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142Y, at pH 7.0 and 75°C

713854

2D-image

0.17

-

mannose 6-phosphate

Sus scrofa

-

-

2828

2D-image

0.2

-

mannose 6-phosphate

Candida albicans

-

Hepes buffer

2825

2D-image

0.23

-

mannose 6-phosphate

Homo sapiens

-

recombinant enzyme

2826

2D-image

0.25

-

mannose 6-phosphate

Homo sapiens

-

wild-type enzyme

2826, 2828

2D-image

0.65

-

mannose 6-phosphate

Saccharomyces cerevisiae

-

-

2828

2D-image

0.73

-

mannose 6-phosphate

Amorphophallus konjac

-

-

2810

2D-image

0.8

-

mannose 6-phosphate

Candida albicans

-

selenomethionine-labelled enzyme, Hepes buffer

2825

2D-image

1

-

mannose 6-phosphate

Candida albicans

-

Tris/HCl buffer

2825

2D-image

1.24

-

mannose 6-phosphate

Candida albicans

-

-

2828

2D-image

1.35

-

mannose 6-phosphate

Saccharomyces cerevisiae

-

-

2624

2D-image

1.6

-

mannose 6-phosphate

Cassia coluteoides

-

enzyme from developing seeds

2813

2D-image

1.9

-

mannose 6-phosphate

Cassia coluteoides

-

enzyme from germinating seeds

2813

2D-image

2

-

mannose 6-phosphate

Xanthomonas campestris

-

-

2827

2D-image

3.03

-

mannose 6-phosphate

Pseudomonas aeruginosa

-

bifunctional enzyme: phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase

2824

2D-image

4

-

mannose 6-phosphate

Candida albicans

-

selenomethionine-labelled enzyme, Tris/HCl buffer

2825

2D-image

additional information

-

additional information

Geobacillus thermodenitrificans

-

enzyme kinetics with D-lyxose and L-ribose, overview

702817

-

3

6

beta-D-mannose 6-phosphate

Homo sapiens

-

in 50 mM HEPES buffer, pH 7.1, at 25°C

716883

-

23

-

beta-D-mannose 6-phosphate

Escherichia coli

-

in 50 mM HEPES buffer, pH 7.1, at 25°C

716883

-

141

-

D-fructose 6-phosphate

Thermus thermophilus

Q5SIM4

wild type enzyme, pH 7.0, 80°C

714372

2D-image

7089

-

D-Lyxose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

2D-image

3748

-

D-mannose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

2D-image

0.076

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

pH 7.1, 25°C

661119

2D-image

0.25

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

mutant R472A, pH 7.0, 25°C

692361

2D-image

0.54

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

mutant R479A, pH 7.0, 25°C

692361

2D-image

0.75

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

mutant R373A, pH 7.0, 25°C

692361

2D-image

1.38

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

wild-type, pH 7.0, 25°C

692361

2D-image

2.63

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

mutant E410A, pH 7.0, 25°C

692361

2D-image

3.25

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

mutant E458A, pH 7.0, 25°C

692361

2D-image

3.5

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

mutant N433A, pH 7.0, 25°C

692361

2D-image

4.41

-

D-mannose 6-phosphate

Pseudomonas aeruginosa

-

mutant H411A, pH 7.0, 25°C

692361

2D-image

11

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme W13A, pH 7.0, 80°C

714372

2D-image

19

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme E132A, pH 7.0, 80°C

714372

2D-image

19.4

-

D-mannose 6-phosphate

Burkholderia cepacia

-, Q06XM8

pH 7.6, presence of 5 mM Mg2+

671785

2D-image

20

-

D-mannose 6-phosphate

Saccharomyces cerevisiae

-

pH 7.1, 25°C

661119

2D-image

31

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme E67A, pH 7.0, 80°C

714372

2D-image

37

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme Q48A, pH 7.0, 80°C

714372

2D-image

54

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme H50A, pH 7.0, 80°C

714372

2D-image

70

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme R142A, pH 7.0, 80°C

714372

2D-image

75

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme H122A, pH 7.0, 80°C

714372

2D-image

1371

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

wild type enzyme, pH 7.0, 80°C

714372

2D-image

23550

-

D-mannose 6-phosphate

Geobacillus thermodenitrificans

A4ITT1, -

wild type enzyme, at pH 7.0 and 70°C

726723

2D-image

55120

-

D-mannose 6-phosphate

Geobacillus thermodenitrificans

A4ITT1, -

mutant enzyme L129F, at pH 7.0 and 70°C

726723

2D-image

85720

-

D-mannose 6-phosphate

Geobacillus thermodenitrificans

A4ITT1, -

mutant enzyme N90A/L129F, at pH 7.0 and 70°C

726723

2D-image

112100

-

D-mannose 6-phosphate

Geobacillus thermodenitrificans

A4ITT1, -

mutant enzyme W17Q/N90A/L129F, at pH 7.0 and 70°C

726723

2D-image

72

-

D-ribose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

2D-image

63970

-

D-talose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

2D-image

920

-

L-allose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

2D-image

17600

-

L-ribose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

2D-image

32670

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142E, at pH 7.0 and 75°C

713854

2D-image

48300

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142Q, at pH 7.0 and 75°C

713854

2D-image

50640

-

L-ribulose

Thermus thermophilus

-

wild type enzyme, at pH 7.0 and 75°C

713854

2D-image

56180

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142Y, at pH 7.0 and 75°C

713854

2D-image

64870

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142A, at pH 7.0 and 75°C

713854

2D-image

68880

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142K, at pH 7.0 and 75°C

713854

2D-image

81060

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142N, at pH 7.0 and 75°C

713854

2D-image

121

-

mannose 6-phosphate

Candida albicans

-

selenomethionine-labelled enzyme

2825

2D-image

162

-

mannose 6-phosphate

Candida albicans

-

wild-type enzyme

2825

2D-image

70

-

beta-D-mannose 6-phosphate

Escherichia coli

-

in 50 mM HEPES buffer, pH 7.1, at 25°C

716883

0

840

-

beta-D-mannose 6-phosphate

Homo sapiens

-

in 50 mM HEPES buffer, pH 7.1, at 25°C

716883

0

641

-

D-fructose 6-phosphate

Thermus thermophilus

Q5SIM4

wild type enzyme, pH 7.0, 80°C

714372

87

16.4

-

D-Lyxose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

1589

3.5

-

D-mannose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

216

13

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme W13A, pH 7.0, 80°C

714372

700

54

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme R142A, pH 7.0, 80°C

714372

700

69

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme Q48A, pH 7.0, 80°C

714372

700

109

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme E132A, pH 7.0, 80°C

714372

700

158

-

D-mannose 6-phosphate

Geobacillus thermodenitrificans

A4ITT1, -

wild type enzyme, at pH 7.0 and 70°C

726723

700

166

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme H50A, pH 7.0, 80°C

714372

700

174

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme E67A, pH 7.0, 80°C

714372

700

227

-

D-mannose 6-phosphate

Geobacillus thermodenitrificans

A4ITT1, -

mutant enzyme L129F, at pH 7.0 and 70°C

726723

700

278

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

mutant enzyme H122A, pH 7.0, 80°C

714372

700

965

-

D-mannose 6-phosphate

Geobacillus thermodenitrificans

A4ITT1, -

mutant enzyme N90A/L129F, at pH 7.0 and 70°C

726723

700

1120

-

D-mannose 6-phosphate

Geobacillus thermodenitrificans

A4ITT1, -

mutant enzyme W17Q/N90A/L129F, at pH 7.0 and 70°C

726723

700

6685

-

D-mannose 6-phosphate

Thermus thermophilus

Q5SIM4

wild type enzyme, pH 7.0, 80°C

714372

700

0.6

-

D-ribose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

292

6.8

-

D-talose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

2017

2.9

-

L-allose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

10165

17.6

-

L-ribose

Bacillus subtilis

-, Q9AGZ4

pH 7.5, 40°C, recombinant enzyme

701797

3127

182

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142Y, at pH 7.0 and 75°C

713854

1621

216

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142E, at pH 7.0 and 75°C

713854

1621

302

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142K, at pH 7.0 and 75°C

713854

1621

322

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142Q, at pH 7.0 and 75°C

713854

1621

353

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142A, at pH 7.0 and 75°C

713854

1621

374

-

L-ribulose

Thermus thermophilus

-

wild type enzyme, at pH 7.0 and 75°C

713854

1621

579

-

L-ribulose

Thermus thermophilus

-

mutant enzyme R142N, at pH 7.0 and 75°C

713854

1621