Literature summary for 5.3.1.8 extracted from

Bernard, A.R.; Wells, T.N.C.; Cleasby, A.; Borlat, F.; Payton, M.A.; Proufoot, A.E.I.
Selenomethionine labelling phosphomannose isomerase changes its kinetic properties (1995), Eur. J. Biochem., 230, 111-118.

Search for more literature for 5.3.1.8

Application

Application	Comment	Organism
pharmacology	absence of mannose-6-phosphate isomerase causes cell lysis and thus the enzyme is a potential target for inhibition and may be a route to antifungal drugs	Candida albicans

Cloned(Commentary)

Cloned (Comment)	Organism
wild-type and selenomethionine-labelled enzyme expressed in Escherichia coli	Candida albicans

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Candida albicans

Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	-	Candida albicans
Hg2+	recombinant wild-type enzyme and selenomethionine-labelled enzyme	Candida albicans
Zn2+	-	Candida albicans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	mannose 6-phosphate	Hepes buffer	Candida albicans
0.8	-	mannose 6-phosphate	selenomethionine-labelled enzyme, Hepes buffer	Candida albicans
1	-	mannose 6-phosphate	Tris/HCl buffer	Candida albicans
4	-	mannose 6-phosphate	selenomethionine-labelled enzyme, Tris/HCl buffer	Candida albicans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48740	-	wild-type recombinant enzyme, electrospray mass spectroscopy	Candida albicans
49060	-	selenomethionine-labelled enzyme expressed in E. coli, electrospray mass spectroscopy	Candida albicans

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	-	wild-type and selenomethionine-labelled enzyme expressed in Escherichia coli	-

Purification (Commentary)

Purification (Comment)	Organism
normal and selenomethionine-labelled enzyme expressed in Escherichia coli	Candida albicans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Candida albicans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Mannose 6-phosphate	-	Candida albicans	D-Fructose 6-phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
121	-	mannose 6-phosphate	selenomethionine-labelled enzyme	Candida albicans
162	-	mannose 6-phosphate	wild-type enzyme	Candida albicans

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Release 2023.1 (January 2023)