Cloned (Comment) | Organism |
---|---|
gene pmi, DNA and amino acid sequence determination and analysis, expression of the His6-tagged enzyme in Escherichia coli strain BL21 (DE3) | Sphingobium chungbukense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP-D-mannose | inhibits mannose 6-phosphate isomerase activity of PMI, feedback regulation of this pathway | Sphingobium chungbukense | |
additional information | no inhibition by GTP, mannose 1-phosphate, and phosphodiphosphate | Sphingobium chungbukense |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Sphingobium chungbukense | |
Co2+ | beste metal ion activator | Sphingobium chungbukense | |
Mg2+ | activates only the GMP activity of the enzyme | Sphingobium chungbukense | |
additional information | PMI absolutely requires divalent metal cations for catalytic activity | Sphingobium chungbukense | |
Ni2+ | activates | Sphingobium chungbukense | |
Zn2+ | activates | Sphingobium chungbukense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, recombinant His6-tagged enzyme, SDS-PAGE | Sphingobium chungbukense |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Mannose 6-phosphate | Sphingobium chungbukense | - |
D-Fructose 6-phosphate | - |
r | |
D-Mannose 6-phosphate | Sphingobium chungbukense DJ77 | - |
D-Fructose 6-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium chungbukense | - |
gene pmi | - |
Sphingobium chungbukense DJ77 | - |
gene pmi | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography | Sphingobium chungbukense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Mannose 6-phosphate | - |
Sphingobium chungbukense | D-Fructose 6-phosphate | - |
r | |
D-Mannose 6-phosphate | - |
Sphingobium chungbukense DJ77 | D-Fructose 6-phosphate | - |
r | |
additional information | PMI is bifunctional possessing both mannose 6-phosphate isomerase and GDP-mannose diphosphorylase activities | Sphingobium chungbukense | ? | - |
? | |
additional information | PMI is bifunctional possessing both mannose 6-phosphate isomerase and GDP-mannose diphosphorylase activities | Sphingobium chungbukense DJ77 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, recombinant His6-tagged enzyme, SDS-PAGE | Sphingobium chungbukense |
More | PMI possesses two domains with three conserved motifs: a GMP domain at the N-terminus and a PMI domain at the C-terminus | Sphingobium chungbukense |
Synonyms | Comment | Organism |
---|---|---|
Phosphomannose isomerase | - |
Sphingobium chungbukense |
PMI | - |
Sphingobium chungbukense |
PMI/GMP | - |
Sphingobium chungbukense |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sphingobium chungbukense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sphingobium chungbukense |
General Information | Comment | Organism |
---|---|---|
metabolism | phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate in the extracellular polysaccharide synthesis pathway, feedback regulation of this pathway | Sphingobium chungbukense |