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Literature summary for 5.3.1.8 extracted from

  • Tran, S.T.; Le, D.T.; Kim, Y.C.; Shin, M.; Choi, J.D.
    Cloning and characterization of phosphomannose isomerase from Sphingomonas chungbukensis DJ77 (2009), BMB Rep., 42, 523-528.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene pmi, DNA and amino acid sequence determination and analysis, expression of the His6-tagged enzyme in Escherichia coli strain BL21 (DE3) Sphingobium chungbukense

Inhibitors

Inhibitors Comment Organism Structure
GDP-D-mannose inhibits mannose 6-phosphate isomerase activity of PMI, feedback regulation of this pathway Sphingobium chungbukense
additional information no inhibition by GTP, mannose 1-phosphate, and phosphodiphosphate Sphingobium chungbukense

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Sphingobium chungbukense
Co2+ beste metal ion activator Sphingobium chungbukense
Mg2+ activates only the GMP activity of the enzyme Sphingobium chungbukense
additional information PMI absolutely requires divalent metal cations for catalytic activity Sphingobium chungbukense
Ni2+ activates Sphingobium chungbukense
Zn2+ activates Sphingobium chungbukense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50000, recombinant His6-tagged enzyme, SDS-PAGE Sphingobium chungbukense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Mannose 6-phosphate Sphingobium chungbukense
-
D-Fructose 6-phosphate
-
r
D-Mannose 6-phosphate Sphingobium chungbukense DJ77
-
D-Fructose 6-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Sphingobium chungbukense
-
gene pmi
-
Sphingobium chungbukense DJ77
-
gene pmi
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Sphingobium chungbukense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Mannose 6-phosphate
-
Sphingobium chungbukense D-Fructose 6-phosphate
-
r
D-Mannose 6-phosphate
-
Sphingobium chungbukense DJ77 D-Fructose 6-phosphate
-
r
additional information PMI is bifunctional possessing both mannose 6-phosphate isomerase and GDP-mannose diphosphorylase activities Sphingobium chungbukense ?
-
?
additional information PMI is bifunctional possessing both mannose 6-phosphate isomerase and GDP-mannose diphosphorylase activities Sphingobium chungbukense DJ77 ?
-
?

Subunits

Subunits Comment Organism
? x * 50000, recombinant His6-tagged enzyme, SDS-PAGE Sphingobium chungbukense
More PMI possesses two domains with three conserved motifs: a GMP domain at the N-terminus and a PMI domain at the C-terminus Sphingobium chungbukense

Synonyms

Synonyms Comment Organism
Phosphomannose isomerase
-
Sphingobium chungbukense
PMI
-
Sphingobium chungbukense
PMI/GMP
-
Sphingobium chungbukense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sphingobium chungbukense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Sphingobium chungbukense

General Information

General Information Comment Organism
metabolism phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate in the extracellular polysaccharide synthesis pathway, feedback regulation of this pathway Sphingobium chungbukense