Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli GI724 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-phospho-D-arabinonhydrazide | - |
Escherichia coli | |
5-phospho-D-arabinonhydrazide | in 50 mM HEPES buffer, pH 7.1, at 25°C | Homo sapiens | |
5-phospho-D-arabinonohydroxamic acid | nanomolar inhibitor | Escherichia coli | |
5-phospho-D-arabinonohydroxamic acid | in 50 mM HEPES buffer, pH 7.1, at 25°C; nanomolar inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00043 | - |
beta-D-mannose 6-phosphate | in 50 mM HEPES buffer, pH 7.1, at 25°C | Homo sapiens | |
0.33 | - |
beta-D-mannose 6-phosphate | in 50 mM HEPES buffer, pH 7.1, at 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc-dependent metalloenzyme | Escherichia coli | |
Zn2+ | zinc-dependent metalloenzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Ni-chelating column chromatography and Q-Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-mannose 6-phosphate | - |
Escherichia coli | beta-D-fructose 6-phosphate | - |
r | |
beta-D-mannose 6-phosphate | - |
Homo sapiens | beta-D-fructose 6-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
type I phosphomannose isomerase | - |
Escherichia coli |
type I phosphomannose isomerase | - |
Homo sapiens |
type I PMI | - |
Escherichia coli |
type I PMI | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 6 | beta-D-mannose 6-phosphate | in 50 mM HEPES buffer, pH 7.1, at 25°C | Homo sapiens | |
23 | - |
beta-D-mannose 6-phosphate | in 50 mM HEPES buffer, pH 7.1, at 25°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000041 | - |
5-phospho-D-arabinonohydroxamic acid | in 50 mM HEPES buffer, pH 7.1, at 25°C | Homo sapiens | |
0.00008 | - |
5-phospho-D-arabinonohydroxamic acid | in 50 mM HEPES buffer, pH 7.1, at 25°C | Escherichia coli | |
0.0006 | - |
5-phospho-D-arabinonhydrazide | in 50 mM HEPES buffer, pH 7.1, at 25°C | Homo sapiens | |
0.002 | - |
5-phospho-D-arabinonhydrazide | in 50 mM HEPES buffer, pH 7.1, at 25°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70 | - |
beta-D-mannose 6-phosphate | in 50 mM HEPES buffer, pH 7.1, at 25°C | Escherichia coli | |
840 | - |
beta-D-mannose 6-phosphate | in 50 mM HEPES buffer, pH 7.1, at 25°C | Homo sapiens |