Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knockout of isoform Pmi2 does not affect the total ascorbic acid levels in leaves | Arabidopsis thaliana |
additional information | reduction of Pmi1 expression by RNA interference results in substantial decrease in the total ascorbic acid content of leaves | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 1 mM, no residual activity | Arabidopsis thaliana | |
dithiothreitol | 1 mM, 73% residual activity; 1 mM, 84% residual activity | Arabidopsis thaliana | |
EDTA | 2.5 mM, 54% residual activity; 2.5 mM, 86% residual activity | Arabidopsis thaliana | |
L-ascorbic acid | 1 mM, 77% residual activity | Arabidopsis thaliana | |
p-chloromercuribenzoate | 0.05 mM, 33% residual activity; 0.05 mM, 39% residual activity | Arabidopsis thaliana | |
Zn2+ | 1 mM, no residual activity | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0413 | - |
D-mannose 6-phosphate | pH 7.5, 25°C | Arabidopsis thaliana | |
0.372 | - |
D-mannose 6-phosphate | pH 7.5, 25°C | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
isoform Pmi1 | - |
Arabidopsis thaliana | - |
isoform Pmi2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | induction of expression under continuous light. Diurnal expression pattern in parallel with the total Pmi1 activity and the total ascorbic acid content in leaf | Arabidopsis thaliana | - |
additional information | constitutive expression in both vegetative and reproductive organs | Arabidopsis thaliana | - |
additional information | no expression in any organ under light. Under long term darkness, expression of isoform Pmi2 with concomitant decrease in ascorbic acid levels | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Mannose 6-phosphate | - |
Arabidopsis thaliana | D-Fructose 6-phosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
- |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Arabidopsis thaliana |