Information on EC 2.3.1.20 - diacylglycerol O-acyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
2.3.1.20
-
RECOMMENDED NAME
GeneOntology No.
diacylglycerol O-acyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
acyl-CoA + 1,2-diacyl-sn-glycerol = CoA + triacylglycerol
show the reaction diagram
-
-
-
-
acyl-CoA + 1,2-diacyl-sn-glycerol = CoA + triacylglycerol
show the reaction diagram
the conserved sequence 161HPHG164 is important for activity
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
triacylglycerol biosynthesis
-
Cutin, suberine and wax biosynthesis
-
Glycerolipid metabolism
-
Metabolic pathways
-
SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
Palmitoyl-CoA and other long-chain acyl-CoAs can act as donors.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1,2-diacylglycerol acyltransferase
-
-
-
-
acyl CoA:diacylglycerol acyltransferase
-
-
acyl CoA:diacylglycerol acyltransferase 1
-
-
acyl coenzyme A:diacylglycerol acyltransferase 1
-
-
acyl coenzyme A:diacylglycerol acyltransferase 1
-
-
acyl coenzyme A:diacylglycerol acyltransferase 2
-
-
acyl-CoA:1,2-dioleoyl-sn-glycerol acyltransferase
-
-
-
-
acyl-CoA:diacylglycerol acyltransferase
Q2KP14
-
acyl-CoA:diacylglycerol acyltransferase
Arachis hypogaea JL 24
Q2KP14
-
-
acyl-CoA:diacylglycerol acyltransferase
Q6ZPD8, Q96PD7
-
acyl-CoA:diacylglycerol acyltransferase
-
-
acyl-CoA:diacylglycerol acyltransferase 1
-
-
acyl-CoA:diacylglycerol acyltransferase 1
-
-
acyl-CoA:diacylglycerol acyltransferase 1
O75907
-
acyl-CoA:diacylglycerol acyltransferase 2
-
-
acyl-CoA:diacylglycerol acyltransferase 2
Q96PD7
-
acyl-CoA:diacylglycerol acyltransferase-2
-
-
acyltransferase, diacylglycerol
-
-
-
-
AtfA
Q8GGG1
enzyme possesses both wax ester synthase and diacylglycerol:acyl-coenzyme A acyltransferase (DGAT) activities
BjDGAT1
Q4U3T9
-
BjDGAT2
Q4U3T8
-
DAG acyltransferase
-
-
DAG acyltransferase
-
-
DC3
Q6ZPD8
-
DGA1
Q08650
gene name
DGA2
Q6C9V5
-
DGAT
-
-
-
-
DGAT
Q2KP14
-
DGAT
Arachis hypogaea JL 24
Q2KP14
-
-
DGAT
Q9XGR5, Q9XGV4
-
DGAT
-
-
DGAT
Q5GKZ7
-
DGAT
-
-
DGAT
Q9DCV3
-
DGAT
Q9Z2A7
-
DGAT
O05879, P0A650, P67208, P67210
-
DGAT
Q9SEG9
-
DGAT
Q67C39
-
DGAT
Q0QJH9, Q0QJI1
-
DGAT
-
-
DGAT1
-
-
-
-
DGAT1
-
-
DGAT1
O75907
-
DGAT1A
-
-
-
-
DGAT2
-
-
-
-
DGAT2
Q96PD7
-
DGAT2
Q9DCV3
-
DGAT2A
-
-
-
-
diacylglycerol acyltransferase
-
-
-
-
diacylglycerol acyltransferase
Q8GGG1
-
diacylglycerol acyltransferase
-
-
diacylglycerol acyltransferase
Q9SLD2
-
diacylglycerol acyltransferase
-
-
diacylglycerol acyltransferase
Q2KP14
-
diacylglycerol acyltransferase
Arachis hypogaea JL 24
Q2KP14
-
-
diacylglycerol acyltransferase
Q9XGR5, Q9XGV4
-
diacylglycerol acyltransferase
-
-
diacylglycerol acyltransferase
-
-
diacylglycerol acyltransferase
-
-
diacylglycerol acyltransferase
Q5GKZ7
-
diacylglycerol acyltransferase
-
-
diacylglycerol acyltransferase
-
-
diacylglycerol acyltransferase
Q9Z2A7
-
diacylglycerol acyltransferase
-
-
diacylglycerol acyltransferase
-
-
diacylglycerol acyltransferase
O05879, P0A650, P67208, P67210
-
diacylglycerol acyltransferase
Q9SEG9
-
diacylglycerol acyltransferase
-
-
diacylglycerol acyltransferase
-
-
diacylglycerol acyltransferase
-
-
diacylglycerol acyltransferase
Q67C39
-
diacylglycerol acyltransferase
-
-
diacylglycerol acyltransferase
Q826D7
-
diacylglycerol acyltransferase
Q9RIU8
-
diacylglycerol acyltransferase
-
-
diacylglycerol acyltransferase 1
Q8MK44
-
diacylglycerol acyltransferase 1
Q4U3T8, Q4U3T9
-
diacylglycerol acyltransferase 1
Q8RX96
-
diacylglycerol acyltransferase 1
B1NM15, B1NM16
-
diacylglycerol acyltransferase-1
-
-
diacylglycerol acyltransferase-1
-
-
diglyceride acyltransferase
-
-
-
-
diglyceride O-acyltransferase
-
-
-
-
EC 2.3.1.124
-
-
formerly
-
FBPA
P0A4V2
gene name
MFAT
-
-
MGAT
-
-
MGAT
-
-
multifunctional O-acyltransferase
-
-
palmitoyl-CoA-sn-1,2-diacylglycerol acyltransferase
-
-
-
-
Rv3088 (TGS4)
P67208
-
Rv3130c (TGS1)
P0A650
-
Rv3234c (TGS3)
O05879
-
Rv3734 (TGS2)
P67210
-
SAV7256
Q826D7
enzyme possesses both wax ester synthase and diacylglycerol:acyl-coenzyme A acyltransferase (DGAT) activities
SCO0958
Q9RIU8
-
TAG synthase
O05879, P0A650, P67208, P67210
-
TmDGAT1
Q8RX96
-
triacylglycerol:acyl-CoA:diacylglycerol acyltransferase
B1NM15, B1NM16
-
type 1 diacylglycerol acyltransferase
Q0QJI1
-
type 2 acyl-CoA:diacylglycerol acyltransferase
-
-
type 2 diacylglycerol acyltransferase
Q0QJH9, Q0QJI1
-
type II diacylglycerol acyltransferase
-
-
unspecific bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase
-
-
wax ester synthase/acyl-CoA:diacylglycerol acyltransferase
-
-
wax ester synthase/acyl-CoA:diacylglycerol acyltransferase
Acinetobacter baylyi ADP1
-
-
-
wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase WSD1
Q93ZR6
bifunctional enzyme
YALI0D07986g
Q6C9V5
ORF name
YALI0D07986g
Yarrowia lipolytica ATCC 20362
Q6C9V5
ORF name
-
YALI0E32769g
Q6C3R2
ORF name
YALI0E32769g
Yarrowia lipolytica ATCC 20362
Q6C3R2
ORF name
-
monoacylglycerol acyltransferase
-
-
additional information
-
cf. EC 2.3.1.75
additional information
-
cf. EC 2.3.1.76
additional information
-
the enzyme belongs to the acyl-CoA:diacylglycerol acyltransferase 2/acyl-CoA:monoacylglycerol acyltransferase, MGAT, gene family
additional information
Q6ZPD8, Q96PD7
cf. EC 2.3.1.75, the enzyme belongs to the acyl-CoA:diacylglycerol acyltransferase, DGAT, 2 gene superfamily
CAS REGISTRY NUMBER
COMMENTARY
9029-98-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain ADP1
-
-
Manually annotated by BRENDA team
Acinetobacter baylyi ADP1
strain ADP1
-
-
Manually annotated by BRENDA team
strain ADP1, formerly Acinetobacter calcoaceticus ADP1
-
-
Manually annotated by BRENDA team
strain ADP1, bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, EC 2.3.1.75 and EC2.3.1.20 resp.
Swissprot
Manually annotated by BRENDA team
strain ADP1, expression in Escherichia coli
Swissprot
Manually annotated by BRENDA team
overexpression of enzyme
Swissprot
Manually annotated by BRENDA team
strain JL 24
SwissProt
Manually annotated by BRENDA team
Arachis hypogaea JL 24
strain JL 24
SwissProt
Manually annotated by BRENDA team
Italian Brown cow
UniProt
Manually annotated by BRENDA team
cv. Reston
-
-
Manually annotated by BRENDA team
cv. Reston and Jet Neuf
-
-
Manually annotated by BRENDA team
cv. Topas
-
-
Manually annotated by BRENDA team
cv. Topas; oilseed rape, cv. Jet Neuf
-
-
Manually annotated by BRENDA team
DGAT1
SwissProt
Manually annotated by BRENDA team
DGAT2
SwissProt
Manually annotated by BRENDA team
oilseed rape, cv. Jet Neuf
-
-
Manually annotated by BRENDA team
var. Dwarf Essex, rapeseed, var. Tower, canola
-
-
Manually annotated by BRENDA team
Cuphea sp.
-
-
-
Manually annotated by BRENDA team
cv. 8904
SwissProt
Manually annotated by BRENDA team
soy bean, Merr. cv. Dare
-
-
Manually annotated by BRENDA team
sunflower, var. Albena
-
-
Manually annotated by BRENDA team
gene DC3; X-linked gene DC3
SwissProt
Manually annotated by BRENDA team
gene DGAT2; gene DGAT2
SwissProt
Manually annotated by BRENDA team
isoform DGAT1
-
-
Manually annotated by BRENDA team
isoform DGAT1
UniProt
Manually annotated by BRENDA team
isoform DGAT2
SwissProt
Manually annotated by BRENDA team
cvs. AC Emerson and Vimy
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
DGAT1
SwissProt
Manually annotated by BRENDA team
DGAT2
SwissProt
Manually annotated by BRENDA team
gene DGAT1; gene DGAT1
SwissProt
Manually annotated by BRENDA team
gene DGAT2; gene DGAT2
SwissProt
Manually annotated by BRENDA team
isoform DGAT2
-
-
Manually annotated by BRENDA team
isoform DGAT2
SwissProt
Manually annotated by BRENDA team
; strain ATCC 25618, gene tgs1 or Rv3130c
-
-
Manually annotated by BRENDA team
induction of several enzymic isoform genes upon entry of cells into nonreplicative drug-resistant state
-
-
Manually annotated by BRENDA team
-
O05879, P0A650, P67208, P67210
SwissProt
Manually annotated by BRENDA team
; male sprague-dawley rats
-
-
Manually annotated by BRENDA team
female Charles River CD
-
-
Manually annotated by BRENDA team
male sprague-dawley rats
-
-
Manually annotated by BRENDA team
male Wistar
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
cv. 99N89I, genes DGAT1 and DGAT2
-
-
Manually annotated by BRENDA team
expression in Saccharomyces cerevisiae
Swissprot
Manually annotated by BRENDA team
isoform DGA1
UniProt
Manually annotated by BRENDA team
isoform DGAT2
UniProt
Manually annotated by BRENDA team
var angulispora, fungus
-
-
Manually annotated by BRENDA team
DGAT1, genomic sequence; tung tree, DGAT1 and DGAT2, both enzymes are encoded by single genes; DGAT1, genomic sequence; tung tree, DGAT1, single gene
SwissProt
Manually annotated by BRENDA team
DGAT2, genomic sequence; tung tree, DGAT2, single gene
SwissProt
Manually annotated by BRENDA team
isoform DGAT1
UniProt
Manually annotated by BRENDA team
isoform DGAT2
UniProt
Manually annotated by BRENDA team
member of the type 1 acyl-CoA:diacylglycerol acyltransferase family
UniProt
Manually annotated by BRENDA team
Yarrowia lipolytica ATCC 20362
isoform DGAT1
UniProt
Manually annotated by BRENDA team
Yarrowia lipolytica ATCC 20362
isoform DGAT2
UniProt
Manually annotated by BRENDA team
maize, Mo17
-
-
Manually annotated by BRENDA team
Zea mays Mo17
maize, Mo17
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
Q08650
overexpression of the enzyme gene in Saccharomyces cerevisiae leads to accumulation of full-length prtein in wild-type and accumulation of full-length protein and a N-terminally truncated protein in a snf2 disruption mutant, lacking a DNA-dependent ATPase that forms the SWI/SNF chromatin remodeling complex. Proteolytic cleavage at the N-terminal region is involved in enzyme activation in the snf2 disruptant, a major cleavage site lies between residues Lys29 and Ser30
physiological function
Q6C9V5
enzyme makes a major contribution to triacylglycerol synthesis via an acyl-CoA-dependent mechanism and is not involved in steryl ester synthesis
physiological function
O75907, Q96PD7
inhibition of isoform DGAT1 affects equally the incorporation of glycerol and exogenous preformed oleate into cellular and secreted triacylglycerol. Data indicate that isoform DGAT2 acts upstream of isoform DGAT1, and DGAT1 functions in the re-esterification of partial glycerides generated by intracellular lipolysis, using preformed fatty acids; isoform DGAT2 activity accounts for a modest fraction of less than 20% of overall cellular DGAT activity. Inhibition of DGAT2 activity specifically inhibits and is rate-limiting for the incorporation of de novo synthesized fatty acids and of glycerol into cellular and secreted triglyceride to a much greater extent than it affects the incorporation of exogenously added oleate. Isoform DGAT2 acts upstream of isoform DGAT1, largely determines the rate of de novo synthesis of triglyceride, and uses nascent diacylglycerol and de novo synthesized fatty acids as substrates
physiological function
P0A4V2
upon overexpression in Mycobacterium smegmatis mc2155, cell morphology is changed and the cells become grossly enlarged. A massive formation of lipid bodies and a change in lipid pattern is observed simultaneously
physiological function
Q6C3R2, Q6C9V5
in isoforms dgat1, dgat2, dgat1/dgat2 double mutant and dgat1/dgat2/phospholipid:diacylglycerol acyltransferase triple mutant the total lipid% dry cell weight as a percentage of the wild-type strain is 57%, 36%, 18% and 13%, respectively; in isoforms dgat1, dgat2, dgat1/dgat2 double mutant and dgat1/dgat2/phospholipid:diacylglycerol acyltransferase triple mutant the total lipid% dry cell weight as a percentage of the wild-type strain is 57%, 36%, 18% and 13%, respectively
physiological function
Yarrowia lipolytica ATCC 20362
-
in isoforms dgat1, dgat2, dgat1/dgat2 double mutant and dgat1/dgat2/phospholipid:diacylglycerol acyltransferase triple mutant the total lipid% dry cell weight as a percentage of the wild-type strain is 57%, 36%, 18% and 13%, respectively; in isoforms dgat1, dgat2, dgat1/dgat2 double mutant and dgat1/dgat2/phospholipid:diacylglycerol acyltransferase triple mutant the total lipid% dry cell weight as a percentage of the wild-type strain is 57%, 36%, 18% and 13%, respectively
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-diacyl-sn-glycerol + erucoyl-CoA
1,2-diacyl-3-erucoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacyl-sn-glycerol + oleoyl-CoA
1,2-diacyl-3-oleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q67C39
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q9XGR5, Q9XGV4
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Cuphea sp.
-
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q9SLD2
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q9SEG9
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q9XGR5, Q9XGV4
DGAT catalyzes the transfer of an acyl moiety between two DAG molecules to form triacylglycerol and monoacylglycerol
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
DGAT1 and DGAT2 are two unrelated enzymes that catalyze the committed step in triacylglycerol biosynthesis
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
DGAT1 catalyzes the committed step in triacylglycerol biosynthesis
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
DGAT2 catalyzes the committed step in triacylglycerol biosynthesis
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q9SLD2
role in leaf metabolism, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q2KP14
the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
acyl-CoA specificity, no acylation activity with phosphoatidylcholine or oleic acid, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
acyl-CoA specificity, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q67C39
microsomal DGAT from castor bean exhibited high activity toward diacylglycerols containing unusual fatty acids, e.g. ricinoleic acids, at both sn-1 and sn-2 positions, acyl-CoA specificity, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Cuphea sp.
-
microsomal DGAT from Cuphea exhibits high activity toward diacylglycerols containing unusual fatty acids, e.g. lauric acids, at both sn-1 and sn-2 positions, acyl-CoA specificity, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Arachis hypogaea JL 24
Q2KP14
the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols
-
-
?
1,2-diacylglycerol + erucoyl-CoA
1,2-diacyl-3-erucoylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + oleoyl-CoA
1,2-diacyl-3-oleoylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + palmitoyl-CoA
1,2-diacyl-3-palmitoylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + stearoyl-CoA
1,2-diacyl-3-stearoylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-dilinolein + alpha-linolenoyl-CoA
1,2-dilinoleoyl-3-alpha-linolenoyl-sn-glycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
-
-
-
?
1,2-dilinolein + linoleoyl-CoA
1,2,3-trilinoleoyl-sn-glycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
-
-
-
?
1,2-dilinolein + oleoyl-CoA
1,2-dilinoleoyl-3-oleoyl-sn-glycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
-
-
-
?
1,2-dilinolein + palmitoyl-CoA
1,2-dilinoleoyl-3-palmitoyl-sn-glycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
-
-
-
?
1,2-dilinolenin + alpha-linolenoyl-CoA
1,2-dilinolenoyl-3-alpha-linolenoyl-sn-glycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
-
-
-
?
1,2-dilinolenin + linoleoyl-CoA
1,2-dilinolenoyl-3-linoleoyl-sn-glycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
-
-
-
?
1,2-dilinolenin + oleoyl-CoA
1,2-dilinolenoyl-3-oleoyl-sn-glycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
-
-
-
?
1,2-dilinolenin + palmitoyl-CoA
1,2-dilinolenoyl-3-palmitoyl-sn-glycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
-
-
-
?
1,2-diolein + alpha-linolenoyl-CoA
1,2-dioleoyl-3-alpha-linolenoyl-sn-glycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
-
-
-
?
1,2-diolein + linoleoyl-CoA
1,2-dioleoyl-3-linoleoyl-sn-glycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
-
-
-
?
1,2-diolein + oleoyl-CoA
1,2,3-trioleoyl-sn-glycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
-
-
-
?
1,2-diolein + palmitoyl-CoA
1,2-dioleoyl-3-palmitoyl-sn-glycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
-
-
-
?
1,2-dioleoyl-sn-glycerol + oleoyl-CoA
triolein + CoA
show the reaction diagram
-
-
-
-
?
1,2-dioleoyl-sn-glycerol + oleoyl-CoA
1,2,3-trioleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-dioleoyl-sn-glycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitol-sn-glycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-dioleoylglycerol + arachidonoyl-CoA
1,2-dioleoyl-3-arachidonoylglycerol + CoA
show the reaction diagram
-
about 8% of the activity with palmitoyl-CoA
-
-
?
1,2-dioleoylglycerol + linoleoyl-CoA
1,2-dioleoyl-3-linoleoylglycerol + CoA
show the reaction diagram
-
about 12% of the activity with palmitoyl-CoA
-
-
?
1,2-dioleoylglycerol + oleoyl-CoA
triolein + CoA
show the reaction diagram
Q6ZPD8, Q96PD7
-
-
-
?
1,2-dioleoylglycerol + oleoyl-CoA
triolein + CoA
show the reaction diagram
-
-
-
-
?
1,2-dioleoylglycerol + oleoyl-CoA
triolein + CoA
show the reaction diagram
-
-
-
-
?
1,2-dioleoylglycerol + oleoyl-CoA
triolein + CoA
show the reaction diagram
Q08650
-
-
-
?
1,2-dioleoylglycerol + oleoyl-CoA
triolein + CoA
show the reaction diagram
-
about 90% of the activity with palmitoyl-CoA
-
-
?
1,2-dioleoylglycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-dioleoylglycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoylglycerol + CoA
show the reaction diagram
Q9DCV3, Q9Z2A7
-
-
-
?
1,2-dioleoylglycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoyl-glycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-dioleoylglycerol + stearoyl-CoA
1,2-dioleoyl-3-stearoylglycerol + CoA
show the reaction diagram
-
about 10% of the activity with palmitoyl-CoA
-
-
?
1,2-dipalmitoyl-rac-glycerol + palmitoyl-CoA
tripalmitoylglycerol + CoA
show the reaction diagram
Q8GGG1
-
-
-
?
1,2-dipalmitoyl-rac-glycerol + palmitoyl-CoA
rac-tripalmitoylglycerol + CoA
show the reaction diagram
P0A4V2
-
-
-
?
1,2-dipalmitoyl-sn-glycerol + oleoyl-CoA
1,2-dipalmitoyl-3-oleoyl-sn-glycerol + CoA
show the reaction diagram
Q2KP14
-
-
-
?
1,2-dipalmitoyl-sn-glycerol + palmitoyl-CoA
tripalmitin + CoA
show the reaction diagram
Q2KP14
-
-
-
?
1,2-dipalmitoyl-sn-glycerol + stearoyl-CoA
1,2-dipalmitoyl-3-stearoyl-sn-glycerol + CoA
show the reaction diagram
Q2KP14
-
-
-
?
1,2-diricinoleoyl-sn-glycerol + ricinoleoyl-CoA
triricinolein + CoA
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-oleoyl glycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-oleoylglycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
show the reaction diagram
-
-
-
-
?
2,3-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
Q9DCV3
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
Q96PD7
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
endogenously and exogenously synthesized diacylglycerol
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
acyl-donors: broad specificity, saturated, mono-, di- and tetraenoic thioesters
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
saturated fatty acyl-CoAs from C-8 to C-12
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
saturated fatty acyl-CoAs from C-4 to C-18
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
Q9Z2A7
acceptors: overview
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
acceptors: 1,2-diacylglycerol containing oleic acid and 1,2-diacylglycerol containing capric acid
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
broad range of donors from C-12 to C-22, broad range of acceptors from C12 to C-22
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
endogenous 1,2-diacylglycerol
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
2,3-diacylglycerol is acylated at 20% the rate of 1,2-isomer
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
last step of triacylglycerol synthesis
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
enzyme catalyzing the final reaction in the sn-glycerol-3-phosphate pathway leading to TAG
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
last reaction in triacylglycerol synthesis
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
DGAT1 is more involved in fat absorption in the intestine and DGAT2 plays important role in assembly of de novo synthesized fatty acids into VLDL particles in liver
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
Zea mays Mo17
-
-
-
-
?
acyl-CoA + 1,2-didecanoylglycerol
CoA + 3-acyl-1,2-didecanoylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-dihexanoylglycerol
CoA + 3-acyl-1,2-dihexanoylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-dilauroylglycerol
CoA + 3-acyl-1,2-dilauroylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-dimyristoylglycerol
CoA + 3-acyl-1,2-dimyristoylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-dioctanoylglycerol
CoA + 3-acyl-1,2-dioctanoylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-dioleoylglycerol
CoA + 3-acyl-1,2-dioleoylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-dioleoylglycerol
CoA + 3-acyl-1,2-dioleoylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-dioleoylglycerol
CoA + 3-acyl-1,2-dioleoylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-dipalmitin
CoA + 3-acyl-1,2-dipalmitoylglycerol
show the reaction diagram
-
no acceptor
-
-
?
acyl-CoA + 1,2-dipalmitoylglycerol
CoA + 3-acyl-1,2-dipalmitoylglycerol
show the reaction diagram
-
membrane bound 1,2-dipalmitoylglycerol
-
-
?
acyl-CoA + 1,3-dioleoylglycerol
CoA + 2-acyl-1,3-dioleoylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,3-dioleoylglycerol
CoA + 2-acyl-1,3-dioleoylglycerol
show the reaction diagram
-
no acceptor
-
-
?
acyl-CoA + 1-decanol
? + CoA
show the reaction diagram
Q8GGG1
at 48% of the activity with hexanol
-
-
?
acyl-CoA + 2,3-dioleoyl-sn-glycerol
CoA + 1-acyl-2,3-dioleoyl-sn-glycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 2-cyclohexylethanol
? + CoA
show the reaction diagram
Q8GGG1
at 45% of the activity with hexanol
-
-
?
acyl-CoA + 2-decanol
? + CoA
show the reaction diagram
Q8GGG1
at 39% of the activity with hexanol
-
-
?
acyl-CoA + 2-monoacylglycerol
CoA + diacylglycerol
show the reaction diagram
-
-
-
-
-
acyl-CoA + 2-oleoylglycerol
CoA + 3-acyl-2-oleoylglyerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 4-decanol
? + CoA
show the reaction diagram
Q8GGG1
at 15.6% of the activity with hexanol
-
-
?
acyl-CoA + cyclododecanol
? + CoA
show the reaction diagram
Q8GGG1
at 80% of the activity with hexanol
-
-
?
acyl-CoA + cyclohexandiol
? + CoA
show the reaction diagram
Q8GGG1
at 4.1% of the activity with hexanol
-
-
?
acyl-CoA + cyclohexanol
? + CoA
show the reaction diagram
Q8GGG1
at 32% of the activity with hexanol
-
-
?
acyl-CoA + cyclohexanone oxime
? + CoA
show the reaction diagram
Q8GGG1
at 5.2% of the activity with hexanol
-
-
?
acyl-CoA + glycerol
CoA + acylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + hexadecanol
? + CoA
show the reaction diagram
Q8GGG1
-
-
-
?
acyl-CoA + hexadecanol
CoA + ?
show the reaction diagram
-
-
-
-
?
acyl-CoA + lysophosphatidylcholine
CoA + acyllysophosphatidylcholine
show the reaction diagram
-
-
-
-
?
acyl-CoA + phenol
? + CoA
show the reaction diagram
Q8GGG1
at 4.1% of the activity with hexanol
-
-
?
acyl-CoA + phenylethanol
? + CoA
show the reaction diagram
Q8GGG1
at 99% of the activity with hexanol
-
-
?
acyl-CoA + rac-1,2-diacetylglycerol
CoA + 3-acyl-rac-1,2-diacetylglycerol
show the reaction diagram
-
no acceptor
-
-
?
acyl-CoA + rac-1,2-dibutyrylglycerol
CoA + 3-acyl-rac-1,2-dibutyrylglycerol
show the reaction diagram
-
no acceptor
-
-
?
acyl-CoA + rac-1,2-dioleoylglycerol
CoA + 3-acyl-rac-1,2-dioleoylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + rac-1,2-dioleoylglycerol
CoA + 3-acyl-rac-1,2-dioleoylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + sn-1,2-diolein
CoA + acyl-sn-1,2-diolein
show the reaction diagram
-
-
-
-
?
acyl-CoA + sn-1,2-diolein
CoA + acyl-sn-1,2-diolein
show the reaction diagram
-
-
-
-
?
acyl-CoA + sn-2-monoolein
CoA + ?
show the reaction diagram
-
-
-
-
?
acyl-CoA + sn-2-monoolein
CoA + ?
show the reaction diagram
-
-
-
-
?
acyl-CoA + sn-2-monoolein
CoA + ?
show the reaction diagram
-
-
-
-
?
arachidonoyl-CoA + 1,2-diacylglycerol
CoA + 3-arachidonoyl-1,2-diacylglycerol
show the reaction diagram
Q9DCV3
-
-
-
?
arachidonoyl-CoA + 1,2-diacylglycerol
CoA + 3-arachidonoyl-1,2-diacylglycerol
show the reaction diagram
-
-
-
-
?
butyryl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-butyrylglycerol
show the reaction diagram
-
-
-
-
?
butyryl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-butyrylglycerol
show the reaction diagram
-
-
-
-
?
butyryl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-butyrylglycerol
show the reaction diagram
-
-
-
-
?
decanoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-decanoylglycerol
show the reaction diagram
-
saturated fatty acyl-CoAs from C-8 to C-18 with decanoyl-CoA as the best
-
-
?
diolein + hexaxosanoyl-CoA
?
show the reaction diagram
-
-
-
-
?
diolein + oleoyl-CoA
triolein + CoA
show the reaction diagram
-
-
-
-
?
erucoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-erucoylglycerol
show the reaction diagram
-
-
-
-
?
erucoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-erucoylglycerol
show the reaction diagram
-
Jet Neuf enzyme displays an enhanced specificity for erucoyl-CoA over oleoyl-CoA
-
-
-
erucoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-erucoylglycerol
show the reaction diagram
Zea mays Mo17
-
-
-
-
?
erucoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 3-erucoyl-1,2-dioleoyl-sn-glycerol
show the reaction diagram
-
-
-
-
?
lauroyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-lauroylglycerol
show the reaction diagram
-
-
-
-
?
lauroyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-lauroylglycerol
show the reaction diagram
-
-
-
-
?
lauroyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-lauroylglycerol
show the reaction diagram
Cuphea carthagenensis, Zea mays Mo17
-
-
-
-
?
linoleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-linoleoylglycerol
show the reaction diagram
Q9DCV3
-
-
-
?
linoleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-linoleoylglycerol
show the reaction diagram
-
-
-
-
?
linoleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-linoleoylglycerol
show the reaction diagram
Q96PD7
-
-
-
?
linoleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-linoleoylglycerol
show the reaction diagram
-
-
-
-
-
linoleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-linoleoylglycerol
show the reaction diagram
-
poor donor
-
-
?
linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 3-linoleoyl-1,2-dioleoylglycerol
show the reaction diagram
-
i.e. diolein
-
?
myristoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-myristoylglycerol
show the reaction diagram
-
-
-
-
?
myristoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-myristoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
show the reaction diagram
Q9Z2A7
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
show the reaction diagram
Q9DCV3
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
show the reaction diagram
Cuphea carthagenensis, Zea mays Mo17
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
show the reaction diagram
-
i.e. diolein, at 34% the rate of the acylation with stearoyl-CoA
-
-
-
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + triolein
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + triolein
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + triolein
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + triolein
show the reaction diagram
B1NM15, B1NM16
oleoyl-CoA and 1,2-dioleoyl-sn-glycerol are preferred substrates over vernoloyl-CoA and 1,2-divernoloyl-sn-glycerol
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
triolein + CoA
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoylglycerol
CoA + 1,2,3-trioleoylglycerol
show the reaction diagram
Q67C39
-
-
-
?
oleoyl-CoA + 1,2-dipalmitoylglycerol
CoA + 3-oleoyl-1,2-dipalmitoylglycerol
show the reaction diagram
Q67C39
-
-
-
?
oleoyl-CoA + 1,2-diricinoloylglycerol
CoA + 3-oleoyl-1,2-diricinoloylglycerol
show the reaction diagram
Q67C39
best substrate
-
-
?
oleoyl-CoA + 1,2-divernoloyl-sn-glycerol
CoA + 1,2-divernoloyl-3-oleoyl-sn-glycerol
show the reaction diagram
B1NM15, B1NM16
oleoyl-CoA and 1,2-dioleoyl-sn-glycerol are preferred substrates over vernoloyl-CoA and 1,2-divernoloyl-sn-glycerol
-
-
?
oleoyl-CoA + diolein
CoA + triolein
show the reaction diagram
O05879, P0A650, P67208, P67210
-
-
-
?
oleoyl-CoA + sn-1,2-dioleoylglycerol
triolein + CoA
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + sn-2-monooleoylglycerol
CoA + 1,2-dioleoyl-sn-glycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
show the reaction diagram
-
diacylglycerol diluted in ethanol
-
-
?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-palmitoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-palmitoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-palmitoylglycerol
show the reaction diagram
-
i.e. diolein
-
-
?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-palmitoyl-sn-glycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-palmitoyl-sn-glycerol
show the reaction diagram
O75907
-
-
-
?
palmitoyl-CoA + 1,2-dipalmitin
CoA + tripalmitin
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,2-dipalmitin
CoA + tripalmitin
show the reaction diagram
Acinetobacter baylyi, Acinetobacter baylyi ADP1
-
-
-
-
?
palmitoyl-CoA + 1,2-dipalmitoylglycerol
CoA + tripalmitin
show the reaction diagram
Q93ZR6
approximately 10fold lower level of diacylglycerol acyltransferase activity
-
-
?
palmitoyl-CoA + 1,2-distearin
CoA + 3-palmitoyl-1,2-distearoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,3-diolein
CoA + 1,3-dioleoyl-2-palmitoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,3-dipalmitin
CoA + tripalmitin
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1-monoolein
CoA + ?
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1-monopalmitin
CoA + ?
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1-monostearin
CoA + ?
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + didecanoylglycerol
CoA + 1,2-didecanoyl-3-palmitoylglycerol
show the reaction diagram
-
i.e. dicaprin
-
-
?
palmitoyl-CoA + dihexanoylglycerol
CoA + dihexanoyl-palmitoylglycerol
show the reaction diagram
-
i.e. dicaproin
-
-
?
palmitoyl-CoA + dipalmitoylglycerol
CoA + tripalmitoylglycerol
show the reaction diagram
-
membrane-bound dipalmitoylglycerol
-
-
?
palmitoyl-CoA + sn-2-monooleoylglycerol
CoA + 1-palmitoyl-2-oleoylglycerol
show the reaction diagram
-
-
-
-
-
ricinoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
1,2-dioleoyl-3-ricinoleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
-
?
ricinoleoyl-CoA + 1,2-dipalmitoyl-sn-glycerol
1,2-dipalmitoyl-3-ricinoleoyl-sn-glycerol + CoA
show the reaction diagram
-
prefers acyl acceptor 1,2-dioleoyl-sn-glycerol over 1,2-dipalmitoyl-sn-glycerol
-
-
?
sn-1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
sn-1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
the final step in triacylglycerol synthesis is catalyzed by the acyl-CoA:diacylglycerol acyltransferase enzymes, DGAT1 and DGAT2, the enzyme contains a neutral lipid binding sequence 80FLVLGVAC87 residing in the first transmembrane domain
-
-
?
sn-1,2-diacylglycerol + erucoyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
i.e. a 22:1cisDELTA13-CoA
-
-
?
stearoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-stearoylglycerol
show the reaction diagram
-
weak donor
-
-
?
stearoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-stearoylglycerol
show the reaction diagram
-
weak donor
-
-
?
stearoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-stearoylglycerol
show the reaction diagram
-
-
-
-
-
stearoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-stearoylglycerol
show the reaction diagram
-
-
-
-
?
stearoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-stearoylglycerol
show the reaction diagram
-
i.e. diolein
-
-
?
vernoloyl-CoA + 1,2-divernoloyl-sn-glycerol
CoA + trivernoloyl-sn-glycerol
show the reaction diagram
B1NM15, B1NM16
oleoyl-CoA and 1,2-dioleoyl-sn-glycerol are preferred substrates over vernoloyl-CoA and 1,2-divernoloyl-sn-glycerol
-
-
?
linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
1,2-dioleoyl-3-linoleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
-
?
additional information
?
-
Q8GGG1
enzyme also has acyl-CoA-monoacylglycerol acyltransferase activity, sn-1 and sn-3 positions are accepted with higher specificity than sn-2 position
-
-
-
additional information
?
-
Q9FNA9
rate of activity is highly dependent on acyl composition with highest activities for acyl groups containing several double bonds, epoxy, or hydroxy groups. Enzyme uses both sn-positions of phosphatidylcholine with 3fold preference for sn-2 position
-
-
-
additional information
?
-
-
in castor bean DGAT2 is more likely to play a major role in seed triacylglycerol biosynthesis than DGAT1
-
-
-
additional information
?
-
-
increased lipid synthesis has variable effects on diacylglycerol accumulation, overview, regulation of lipid biosynthesis in cultures tissues, changes in the endogenous activity of DAGAT is unlikely to affect oil accumulation in oil palm crops, overview
-
-
-
additional information
?
-
Q9DCV3, Q9Z2A7
increasing the cytoplasmic triglyceride pool in hepatocytes does not directly influence VLDL triglyceride or apoB production, overview
-
-
-
additional information
?
-
-
mechanism for the degradation of the DGAT protein, overview
-
-
-
additional information
?
-
-
the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols
-
-
-
additional information
?
-
-
the multifunctional enzyme plays an important role in lipid metabolism in human skin
-
-
-
additional information
?
-
-
acyl-CoA specificity, overview
-
-
-
additional information
?
-
Q9XGR5, Q9XGV4
acyl-CoA specificity, overview
-
-
-
additional information
?
-
Q9SLD2
acyl-CoA specificity, overview
-
-
-
additional information
?
-
-
DGAT is also catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22
-
-
-
additional information
?
-
-
DGAT is catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the diacylglycerol transferase, EC 2.3.1.20, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview
-
-
-
additional information
?
-
Q9DCV3, Q9Z2A7
DGAT1 is also catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview
-
-
-
additional information
?
-
-
DGAT1 is catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the diacylglycerol transferase, EC 2.3.1.20, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview
-
-
-
additional information
?
-
Q9DCV3, Q9Z2A7
DGAT1 is catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the diacylglycerol transferase, EC 2.3.1.20, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview
-
-
-
additional information
?
-
-
no activity with sn-1,3-diacylglycerols, acyl-CoA specificity, overview
-
-
-
additional information
?
-
-
no activity with sn-1,3-diacylglycerols, water-soluble diacylglycerols, including rac-1,2-diacetylglycerol and rac-1,2-dibutyrylglycerol, are poor substrates of DGAT from safflower seeds, acyl-CoA specificity, overview
-
-
-
additional information
?
-
Q2KP14
substrate specificity of native and recombinant enzyme, the native and the recombinant enzyme do not show wax ester synthase activity in contrast to other DGATs, no activity with hexadecanol, glycerol-3-phosphate, monoacylglycerol, lysophosphatidic acid, and lysophosphatidylcholine, oleoyl-CoA is the preferred acyl donor as compared to palmitoyl- and stearoyl-CoAs
-
-
-
additional information
?
-
-
the bifunctional enzyme catalyzes the reactions of the diacylglycerol transferase, EC 2.3.1.20, and of the wax synthase, EC 2.3.1.75
-
-
-
additional information
?
-
-
the enzyme also performs the reaction of the retinol O-fatty-acyltransferase, EC 2.3.1.76
-
-
-
additional information
?
-
Q8GGG1
ATfA exhibits a clear preference for the acylation of the sn-2 position of sn-1,2-dipalmitoylglycerol rather than the sn-2 position of sn-1,3-dipalmitoylglycerol
-
-
-
additional information
?
-
Q8RX96, -
recombinant TmDGAT1 protein is capable of utilizing a range of (14)C-labelled fatty acyl-CoA donors and diacylglycerol acceptors, and can synthesize (14)C-trierucin
-
-
-
additional information
?
-
-
enzyme displays striking preference to palmitoyl-CoA and oleoyl-CoA as acyl donors and utilizes 1,2-diacylglycerol as acceptor
-
-
-
additional information
?
-
P0A4V2
enzyme mediates the transesterification of diacylglycerol using long-chain acyl-CoA as acyl donors. In addition, it functions as mycolyltransferase, a docking model suggests that palmitoleoyl-coenzyme A and 1,2-dipalmitin occupy the same active site as trehalose 6,6-dimycolate and trehalose 6-monomycolate
-
-
-
additional information
?
-
Arachis hypogaea JL 24
Q2KP14
substrate specificity of native and recombinant enzyme, the native and the recombinant enzyme do not show wax ester synthase activity in contrast to other DGATs, no activity with hexadecanol, glycerol-3-phosphate, monoacylglycerol, lysophosphatidic acid, and lysophosphatidylcholine, oleoyl-CoA is the preferred acyl donor as compared to palmitoyl- and stearoyl-CoAs
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q67C39
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Mus musculus, Cuphea sp.
-
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q9SEG9
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q9XGR5, Q9XGV4
DGAT catalyzes the transfer of an acyl moiety between two DAG molecules to form triacylglycerol and monoacylglycerol
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
DGAT1 and DGAT2 are two unrelated enzymes that catalyze the committed step in triacylglycerol biosynthesis
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
DGAT1 catalyzes the committed step in triacylglycerol biosynthesis
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q0QJH9, Q0QJI1, -
DGAT2 catalyzes the committed step in triacylglycerol biosynthesis
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Q9SLD2
role in leaf metabolism, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
Arachis hypogaea, Arachis hypogaea JL 24
Q2KP14
the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols
-
-
?
1,2-dioleoylglycerol + oleoyl-CoA
triolein + CoA
show the reaction diagram
-
-
-
-
?
1,2-dioleoylglycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-dioleoylglycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoylglycerol + CoA
show the reaction diagram
Q9DCV3, Q9Z2A7
-
-
-
?
1,2-diricinoleoyl-sn-glycerol + ricinoleoyl-CoA
triricinolein + CoA
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
last step of triacylglycerol synthesis
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
enzyme catalyzing the final reaction in the sn-glycerol-3-phosphate pathway leading to TAG
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
last reaction in triacylglycerol synthesis
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
DGAT1 is more involved in fat absorption in the intestine and DGAT2 plays important role in assembly of de novo synthesized fatty acids into VLDL particles in liver
-
-
?
palmitoyl-CoA + sn-2-monooleoylglycerol
CoA + 1-palmitoyl-2-oleoylglycerol
show the reaction diagram
-
-
-
-
-
sn-1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
sn-1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
the final step in triacylglycerol synthesis is catalyzed by the acyl-CoA:diacylglycerol acyltransferase enzymes, DGAT1 and DGAT2
-
-
?
acyl-CoA + 2-monoacylglycerol
CoA + diacylglycerol
show the reaction diagram
-
-
-
-
-
additional information
?
-
-
in castor bean DGAT2 is more likely to play a major role in seed triacylglycerol biosynthesis than DGAT1
-
-
-
additional information
?
-
-
increased lipid synthesis has variable effects on diacylglycerol accumulation, overview, regulation of lipid biosynthesis in cultures tissues, changes in the endogenous activity of DAGAT is unlikely to affect oil accumulation in oil palm crops, overview
-
-
-
additional information
?
-
Q9DCV3, Q9Z2A7
increasing the cytoplasmic triglyceride pool in hepatocytes does not directly influence VLDL triglyceride or apoB production, overview
-
-
-
additional information
?
-
-
mechanism for the degradation of the DGAT protein, overview
-
-
-
additional information
?
-
-
the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols
-
-
-
additional information
?
-
-
the multifunctional enzyme plays an important role in lipid metabolism in human skin
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
activation, to some extent
Co2+
-
activation to some extent
Mg2+
-
1.4fold stimulation
Mg2+
-
2fold stimulation at 20 mM
Mg2+
-
stimulation but no absolute requirement
Mg2+
-
2-10 mM; omission results in 50% activity
Mg2+
-
strongly dependent
Mg2+
-
2.4fold at 8 mM
Mg2+
-
stimulation at 3 and 10 mM
Mg2+
Q6ZPD8, Q96PD7
;
Mg2+
Q2KP14
-
Mn2+
-
2fold stimulation at 20 mM
Mn2+
-
stimulation but no absolute requirement
NaCl
-
1.4fold stimulation at 0.5 M
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(2alpha,3beta)-2,3-dihydroxyurs-12-en-28-oic acid
-
IC50: 0.0443 mM
(2E)-1-[2,4-dihydroxy-6-methoxy-3-[(2R)-5-methyl-2-(1-methylethenyl)hex-4-en-1-yl]phenyl]-3-(2,4-dihydroxyphenyl)prop-2-en-1-one
-
IC50: 0.0098 mM
(2E,4Z,8E)-N-[9-(3,4-methylenedioxyphenyl)-2,4,8-nonatrienoyl]piperidine
-
IC50: 0.0298 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
(2R,3R)-2-(2,4-Dihydroxy-phenyl)-3,7-dihydroxy-8-((R)-5-hydroxy-2-isopropenyl-5-methyl-hexyl)-5-methoxy-chroman-4-one
-
i.e. kushenol H, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.142 mM
(2R,3S)-2-(2,4-Dihydroxy-phenyl)-3,7-dihydroxy-8-((R)-5-hydroxy-2-isopropenyl-5-methyl-hexyl)-5-methoxy-chroman-4-one
-
i.e. kushenol K, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.250 mM
(trans-4-(4-[(5-benzyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
inhibitor displays good metabolic stability and high intestinal permeability values
(trans-4-(4-[(5-cyclopentylethyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
-
(trans-4-(4-[(5-cyclopentylmethyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
-
(trans-4-(4-[(5-[1-fluorobenzyl]-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
inhibitor displays good metabolic stability and high intestinal permeability values
(trans-4-(4-[(5-[2-chlorobenzyl]-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
inhibitor displays good metabolic stability and high intestinal permeability values
(trans-4-(4-[(5-[2-fluorobenzyl]-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
inhibitor displays good metabolic stability and high intestinal permeability values
1,2-diacyl-sn-glycerol
-
dicaprin, dimyristin, dipalmitin, high concentration
1-acyl-sn-glycero-3-phosphocholine
-
activation, at low concentrations, inhibition above 0.2 mM
1-O-Hexadecyl-2-oleoyl-sn-glycerol
-
competitive
1-O-Hexadecylpropanediol-3-phosphocholine
-
activation, low concentration, inhibitory at high concentration
1-[2,4-dihydroxy-3-(2-isopropenyl-5-methyl-hex-4-enyl)-6-methoxy-phenyl]-3-(2,4-dihydroxy-phenyl)-propenone
-
i.e. kuraridin, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.099 mM
2-(2,4-dihydroxy-phenyl)-7-hydroxy-8-(2-isopropenyl-5-methyl-hex-4-enyl)-5-methoxy-chroman-4-one
-
i.e. kurarinone, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.0109 mM
2-(2,4-dihydroxy-phenyl)-7-hydroxy-8-[2-(3-hydroxy-3-methyl-butyl)-3-methyl-but-3-enyl]-5-methoxy-chroman-4-one
-
i.e. kurarinol, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.0086 mM
2-Bromooctanoate
-
50% inhibition at 1.5 mM
2-Bromooctanoate
-
specific inhibition
2-Bromooctanoate
Q9XGR5, Q9XGV4
;
5,5'-dithio-bis(2-nitrobenzoic acid)
-
i.e. DTNB, complete inhibition, DTT protects
5,5'-dithio-bis(2-nitrobenzoic acid)
-
11% inhibition at 1 mM
6,8-diprenylgenistein
-
IC50: 0.0067 mg/ml
7beta-(3-ethyl-cis-crotonoyloxy)-1alpha-(2-methylbutyryloxy)-3,14-dehydro-Z-notonipetranone
-
-
8-angeloyloxy-3,4-epoxy-bisabola-7(14),10-dien-2-one
-
-
8-prenylleutone
-
IC50: 0.015 mg/ml
acyl-CoA
-
C-10-CoA to C-16-CoA, high concentration
alpinumisoflavone
-
23% inhibition at 0.0125 mg/ml
amidepsine A
O75907
;
amidepsine B
O75907
;
amidepsine C
O75907
;
-
amidepsine D
O75907
;
ATP
-
ATP-dependent activity reduces activity in vitro by 30-40%
auriculatin
-
IC50: 0.0072 mg/ml
betulinic acid
-
a lupane-type triterpenoid, isolated from methanolic extracts of Alnus hirsuta, inhibition of Hep-G2 cell triacyglycerol biosynthesis
betulinic acid
-
a lupane-type triterpenoid, isolated from methanolic extracts of Alnus hirsuta, noncompetitive inhibition, IC50: 0.0096 mM
Bovine serum albumin
-
binds palmitoyl-CoA and decreases activity towards palmitoyl-CoA, but not butyryl-CoA
-
Bovine serum albumin
-
activation at 0.1%, inhibition at 2.5%
-
Bovine serum albumin
-
strong inhibition
-
brachynereolide
-
IC50: 0.25 mM, above
Butyryl-CoA
-
-
Ca2+
-
48% inhibition at 2.5 mM
CaCl2
-
2 mM, inhibits purified enzyme but not activity in lipid body fraction
CaCl2
Q8GGG1
5 mM, 30% inhibition
Cetyltrimethylammonium bromide
-
-
cholate
-
not up to 4 mM
CoA
Q8GGG1
above 50 mM, about 70% inhibition above 300 mM
crepidiaside C
-
IC50: 0.25 mM, above
cryptotanshinone
-
IC50: 0.0273 mM
dehydropipernonaline
-
noncompetitive inhibition, IC50: 0.0212 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
deoxycholate
-
-
deoxycholate
-
-
derrone
-
IC50: 0.0151 mg/ml
DTT
-
above 2 mM
DTT
-
microsomal preparation
erysenegalensein D
-
IC50: 0.0015 mg/ml
erysenegalensein N
-
28% inhibition at 0.0125 mg/ml
erysenegalensein O
-
IC50: 0.0011 mg/ml
ethanol
-
43% inhibition when 0.04 ml are added to reaction mixture
germanicol acetate
-
IC50: 0.25 mM, above
Gum arabic
-
-
-
HgCl2
-
90% at 0.03-0.05 mM, reversible by DTT
I-
-
substantial inhibition
Iodide
Q9XGR5, Q9XGV4
;
iodoacetamide
-
15% inhibition at 1 mM, DTT protects
ixerin Y
-
IC50: 0.25 mM, above
KCl
-
500 mM, inhibits purified enzyme
kuraridin
-
IC50: 0.0098 mM
kurarinone
-
IC50: 0.0048 mg/ml
lysophosphatidylcholine
-
activation at low concentrations, optimum at 0.075 mM, inhibitory above 0.2 mM
methyl ursolate
-
IC50: 0.0264 mM
Mg2+
-
10% inhibition at 2.5 mM, 40% at 8.0 mM
MgCl2
-
20 mM
MgCl2
Q9DCV3
100 mM
MgCl2
-
5 mM, inhibits purified enzyme but not activity in lipid body fraction
MgCl2
Q8GGG1
5 mM, 17% inhibition
Mn2+
-
73% inhibition at 2.5 mM
N-(4,5-dihydronaphtho[1,2-d]thiazol-2-yl)-2-(3,4-dimethoxy phenyl)acetamide
O75907, Q96PD7
selective inhibitor of isoform DGAT2 when used at low concentrations; selective inhibitor of isoform DGAT2 when used at low concentrations
-
N-ethylmaleimide
-
inhibits solubilized enzyme, but not membrane-bound enzyme
N-ethylmaleimide
-
95% loss of activity after preincubation with 40 mM
n-octyl-beta-D-glucopyranoside
-
weak
niacin
-
30% inhibition
oleanolic acid
-
IC50: 0.0317 mM
oleate
-
noncompetitive inhibition, IC50: 0.0545 mM
oleoyl-CoA
-
substrate inhibition
p-chloromercuribenzene sulfonate
-
complete inhibition, DTT protects
p-chloromercuribenzoate
-
complete inhibition, DTT protects
palmitoyl-CoA
-
high concentration
phenylpyropene A
-
-
phenylpyropene B
-
-
phenylpyropene C
-
noncompetitive
pipernonaline
-
IC50: 0.0372 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
piperrolein B
-
IC50: 0.0201 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
retrofractamide C
-
IC50: 0.9 mM, above, isolated from the extracts of fruits of Piper longum and Piper nigrum
roselipin 1a
O75907
;
roselipin 1B
O75907
;
roselipin 2A
O75907
;
roselipin 2B
O75907
;
sesquiterpenoids
-
weak inhibition
-
sodium taurocholate
-
-
sodiumdodecyl sulfate
-
-
sodiumdodecyl sulfate
-
-
sodiumdodecyl sulfate
-
irreversible denaturation
Soybean trypsin inhibitor
Q9XGR5, Q9XGV4
;
-
tannin
Q9XGR5, Q9XGV4
;
-
Tergitol NP-40
-
microsomal preparation
Trifluoperazine
-
0.5 mM, in the presence of suboptimal phosphatidic acid concentration
Triton X-100
-
above 0.05 mM
Triton X-100
-
microsomal preparation
Triton X-100
-
microsomal preparation
Triton X-100
-
50% inhibition at 0.1-0.2%
Tween 20
-
99% inhibition at 5 mg/ml, 89% inhibition at 1 mg/ml, 49% inhibition at 0.5 mg/ml
xanthohumol
O75907
;
XP620
-
; dihydrothiopyrancarboxamide
XP620
-
dihydrothiopyrancarboxamide
MnCl2
Q8GGG1
5 mM, 30% inhibition
additional information
-
no inhibition by Ca2+
-
additional information
-
10% v/v acetone, sn-2-monoolein
-
additional information
-
diethyl-p-nitrophenylphosphate, diisopropyl fluorophosphate
-
additional information
-
not inhibitory: hesperetin, naringenin, quercetin, kaempferol up to 0.8 mM
-
additional information
-
an ethylacetic extract of Youngia koidzumiana, a plant endemic to the Mount Chiri region of Korea, significantly inhibits the liver microsome enzyme
-
additional information
-
inhibition mechanism, the inhibitory activity of Piper fruits is influenced by the presence of the piperidine group rather than by isobutyl group, overview
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1-acyl-sn-glycero-3-phosphocholine
-
activation, at low concentrations, inhibition above 0.2 mM
1-Acyl-sn-glycero-3-phosphoethanolamine
-
activation, to a lesser extent
1-O-Hexadecylpropanediol-3-phosphocholine
-
activation, low concentration, inhibitory at high concentration
ammonium sulfate
Q8GGG1
45 mM, 10% increase in activity
ATP
Q9XGR5, Q9XGV4
;
Bovine serum albumin
-
activation, 1.0-1.5 mg/ml
-
Bovine serum albumin
-
65% activation at 1 mg/ml, microsomal preparation
-
Bovine serum albumin
-
4-5fold by 3 to 4 mg/ml
-
Bovine serum albumin
-
activation at 0.1%, inhibition at 2.5%
-
Bovine serum albumin
-
activates
-
Bovine serum albumin
Q9XGR5, Q9XGV4
5fold at 3-4 mg/ml; 5fold at 3-4 mg/ml
-
CoA
Q9XGR5, Q9XGV4
;
DTT
-
activation, microsomal preparation
ethanol
-
highest activity when diacylglycerol is in ethanol
glucose
-
treatment of adipocytes with glucose increases DGAT1 mRNA
Insulin
-
treatment of adipocytes with insulin increases DGAT2 mRNA
-
phosphatidic acid
-
-
phosphatidylcholine
-
from egg yolk
phosphocholine
-
from soy bean, activation
Phospholipid
-
microsomal
Phospholipid
-
-
Sucrose
-
treatment of cells with elevated sucrose levels increases DGAT activity
lysophosphatidylcholine
-
activation at low concentrations, optimum at 0.075 mM, inhibitory above 0.2 mM
additional information
Q9SLD2
effectivity of detergents used in in vitro assays, overview
-
additional information
Q9XGR5, Q9XGV4
effectivity of detergents used in in vitro assays, overview; effectivity of detergents used in in vitro assays, overview
-
additional information
-
effectivity of detergents used in in vitro assays, overview
-
additional information
Q67C39
effectivity of detergents used in in vitro assays, overview
-
additional information
-
effectivity of detergents used in in vitro assays, overview
-
additional information
-
tgs transcript levels are upregulated under various stress culture conditions
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.014
-
1,2-diolein
-
-
0.016
0.025
Butyryl-CoA
-
-
0.028
-
diolein
O05879, P0A650, P67208, P67210
-
0.714
-
diolein
O05879, P0A650, P67208, P67210
-
0.0046
-
oleoyl-CoA
-
pH 8.0, 25C
0.008
-
oleoyl-CoA
-
-
0.0133
-
oleoyl-CoA
-
-
0.026
-
oleoyl-CoA
O05879, P0A650, P67208, P67210
-
0.06
-
oleoyl-CoA
-
-
0.667
-
oleoyl-CoA
O05879, P0A650, P67208, P67210
-
0.006
-
palmitoyl-CoA
-
-
0.0211
-
palmitoyl-CoA
Q8GGG1
pH 7.4, 35C
0.39
-
palmitoyl-CoA
P0A4V2
pH 7.0, 37C
0.006
-
sn-1,2-diolein
-
with stearoyl-CoA
0.113
-
sn-1,2-diolein
-
with oleoyl-CoA
0.0057
0.0068
Hexanoyl-CoA
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
acyl-CoA substrate binding kinetics of the recombinant N-terminal fragment, overview
-
additional information
-
additional information
-
the flux control coefficient is 0.12 in oil palm
-
additional information
-
additional information
-
the flux control coefficient is 0.74 in olive
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.92
-
palmitoyl-CoA
P0A4V2
pH 7.0, 37C
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0054
-
oleoyl-CoA
-
pH 8.0, 25C
0.0081
-
oleoyl-CoA
-
-
0.0104
-
phenylpyropene C
-
-
0.032
-
1-O-Hexadecyl-2-oleoyl-sn-glycerol
-
-
additional information
-
additional information
-
inhibition kinetics
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0443
-
(2alpha,3beta)-2,3-dihydroxyurs-12-en-28-oic acid
-
IC50: 0.0443 mM
0.0098
-
(2E)-1-[2,4-dihydroxy-6-methoxy-3-[(2R)-5-methyl-2-(1-methylethenyl)hex-4-en-1-yl]phenyl]-3-(2,4-dihydroxyphenyl)prop-2-en-1-one
-
IC50: 0.0098 mM
0.0298
-
(2E,4Z,8E)-N-[9-(3,4-methylenedioxyphenyl)-2,4,8-nonatrienoyl]piperidine
-
IC50: 0.0298 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
0.00036
-
(trans-4-(4-[(5-benzyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
pH not specified in the publication, 25C
0.000019
-
(trans-4-(4-[(5-cyclopentylethyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
pH not specified in the publication, 25C
0.000024
-
(trans-4-(4-[(5-cyclopentylmethyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
pH not specified in the publication, 25C
0.000045
-
(trans-4-(4-[(5-[1-fluorobenzyl]-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
pH not specified in the publication, 25C
0.00003
-
(trans-4-(4-[(5-[2-chlorobenzyl]-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
pH not specified in the publication, 25C
0.00003
-
(trans-4-(4-[(5-[2-fluorobenzyl]-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
pH not specified in the publication, 25C
0.047
-
7beta-(3-ethyl-cis-crotonoyloxy)-1alpha-(2-methylbutyryloxy)-3,14-dehydro-Z-notonipetranone
-
-
0.1607
-
7beta-(3-ethyl-cis-crotonoyloxy)-1alpha-(2-methylbutyryloxy)-3,14-dehydro-Z-notonipetranone
-
-
0.211
-
8-angeloyloxy-3,4-epoxy-bisabola-7(14),10-dien-2-one
-
-
0.2944
-
8-angeloyloxy-3,4-epoxy-bisabola-7(14),10-dien-2-one
-
-
0.04
-
amidepsine A
O75907
-
0.07
-
amidepsine A
O75907
-
0.03
-
amidepsine B
O75907
-
0.2
-
amidepsine C
O75907
-
-
0.02
-
amidepsine D
O75907
-
0.03
-
amidepsine D
O75907
-
0.0096
-
betulinic acid
-
a lupane-type triterpenoid, isolated from methanolic extracts of Alnus hirsuta, noncompetitive inhibition, IC50: 0.0096 mM
0.25
-
brachynereolide
-
IC50: 0.25 mM, above
0.25
-
crepidiaside C
-
IC50: 0.25 mM, above
0.0273
-
cryptotanshinone
-
IC50: 0.0273 mM
0.0212
-
dehydropipernonaline
-
noncompetitive inhibition, IC50: 0.0212 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
0.25
-
germanicol acetate
-
IC50: 0.25 mM, above
0.25
-
ixerin Y
-
IC50: 0.25 mM, above
0.0098
-
kuraridin
-
IC50: 0.0098 mM
0.0098
-
kuraridin
-
-
0.0109
-
kurarinone
-
-
0.0288
-
kurarinone
-
-
0.0085
-
N-(4,5-dihydronaphtho[1,2-d]thiazol-2-yl)-2-(3,4-dimethoxy phenyl)acetamide
O75907, Q96PD7
pH not specified in the publication, temperature not specified in the publication
-
0.0708
-
N-(4,5-dihydronaphtho[1,2-d]thiazol-2-yl)-2-(3,4-dimethoxy phenyl)acetamide
O75907, Q96PD7
pH not specified in the publication, temperature not specified in the publication
-
0.0317
-
oleanolic acid
-
IC50: 0.0317 mM
0.0545
-
oleate
-
noncompetitive inhibition, IC50: 0.0545 mM
0.0787
-
phenylpyropene A
-
-
0.0217
-
phenylpyropene B
-
-
0.01104
-
phenylpyropene C
-
-
0.0372
-
pipernonaline
-
IC50: 0.0372 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
0.0201
-
piperrolein B
-
IC50: 0.0201 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
0.05
-
roselipin 2B
O75907
-
0.0188
-
tussilagone
-
-
0.0491
-
tussilagone
-
-
0.0992
-
tussilagonone
-
-
0.0000014
-
XP620
-
substrate: 1-hexadecanol
0.0000027
-
XP620
-
substrate: sn-2-monooleoylglycerol
0.0000029
-
XP620
-
substrate: sn-2-monooleoylglycerol
0.0000166
-
XP620
-
substrate: sn-2-monooleoylglycerol
0.0000233
-
XP620
-
substrate: 1-hexadecanol
0.000123
-
XP620
-
substrate: 1,2-dioleoyl-sn-glycerol
0.000176
-
XP620
-
substrate: 1,2-dioleoyl-sn-glycerol
0.001499
-
XP620
-
substrate: 1,2-dioleoyl-sn-glycerol
0.06
-
amidepsine B
O75907
-
additional information
-
amidepsine C
O75907
IC50 above 0.2 mM
-
0.0264
-
methyl ursolate
-
IC50: 0.0264 mM
additional information
-
additional information
-
kurarione IC50: 0.0048 mg/ml, 8-prenylleutone IC50: 0.015 mg/ml, auriculatin IC50: 0.0072 mg/ml, erysenegalensein O IC50: 0.0011 mg/ml, erysenegalensein D IC50: 0.0015 mg/ml, erysenegalensein N: 28% inhibition at 0.0125 mg/ml, derrone IC50: 0.0151 mg/ml, alpinumisoflavone: 23% inhibition at 0.0125 mg/ml, 6,8-diprenylgenistein IC50: 0.0067 mg/ml
-
additional information
-
additional information
-
IC50 value for tussilagonone above 0.5 mM
-
0.9
-
retrofractamide C
-
IC50: 0.9 mM, above, isolated from the extracts of fruits of Piper longum and Piper nigrum
additional information
-
roselipin 1a
O75907
IC50 above 0.2 mM
0.03
-
roselipin 1a
O75907
-
additional information
-
roselipin 1B
O75907
IC50 above 0.2 mM
0.04
-
roselipin 1B
O75907
-
additional information
-
roselipin 2A
O75907
IC50 above 0.2 mM
0.045
-
roselipin 2A
O75907
-
additional information
-
roselipin 2B
O75907
IC50 above 0.2 mM
0.04
-
xanthohumol
O75907
;
additional information
-
XP620
-
IC50 above 0.03 mM with 1,2-dioleoyl-sn-glycerol or sn-2-monooleoylglycerol as substrate
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0003
-
-
membrane fraction after insulin-starvation
0.00036
-
-
membrane fraction after carbohydrate-starvation
0.0012
-
-
-
0.0024
0.038
-
-
0.00265
-
-
membrane fraction
0.00292
-
-
after KCl wash
0.0035
0.0134
-
transfected cells
0.00361
-
-
-
0.0038
-
-
submandibular gland
0.00391
-
-
parotid gland
0.0071
-
Q2KP14
purified native enzyme
0.0073
-
-
after stimulation with cytosolic fraction
0.0089
-
-
-
0.0098
-
-
microsomal fraction of embryo liver after 12 days of development
0.0225
-
-
microsomal fraction of embryo liver after 19 days of development
0.0264
-
-
microsomal fraction of embryo liver after 22 days of development
0.05
-
-
; above
0.0543
-
Q8GGG1
pH 7.4, 35C
0.119
-
-
-
0.169
0.354
Q9DCV3
-
0.3
-
Q8GGG1
-
additional information
-
-
specific activity in the nanomolar range in microsomal preparations, varies considerably between different tissues
additional information
-
-
microsomal preparations with detergents
additional information
-
-
-
additional information
-
-
-
additional information
-
-
specific activity in homogenates in the picomolar range
additional information
-
-
total enzyme activity derived from dehydrated muscle tissue is similar to, or better than, the total activity of enzyme from fresh tissue
additional information
-
-
values of enzyme activity for several isoforms
additional information
-
-
shows DGAT activity when expressed in recombinant Escherichia coli
additional information
-
O05879, P0A650, P67208, P67210
high DGAT activity when expressed in recombinant Escherichia coli; high DGAT activity when expressed in recombinant Escherichia coli; high DGAT activity when expressed in recombinant Escherichia coli; high DGAT activity when expressed in recombinant Escherichia coli
additional information
-
Q826D7
shows DGAT activity when expressed in recombinant Escherichia coli
additional information
-
Q9RIU8
does not show any DGAT activity when expressed in recombinant Escherichia coli
additional information
-
Q9DCV3
; recombinant enzyme in Sf9 cell membrane and in COS-7 cell membranes, multifunctional enzyme
additional information
-
Q9DCV3, Q9Z2A7
plasma and liver lipid levels of wild-type mice compared to recombinant DGAT1 overexpressing mice; plasma and liver lipid levels of wild-type mice compared to recombinant DGAT2 overexpressing mice
additional information
-
-
lipid content and fatty acid composition of triacylglycerols during seed development, overview
additional information
-
-
quantitative expression analysis of DGAT1 and DGAT2
additional information
-
Q2KP14
substrate specificity of native and recombinant enzyme
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.1
-
-
broad
6.6
-
-
-
7
9
-
broad
7.4
-
-
assay at
7.4
-
-
assay at
7.4
-
-
assay at
7.5
-
Q6ZPD8, Q96PD7
assay at; assay at
7.5
-
-
assay at
7.5
-
Q2KP14
assay at
7.8
-
-
assay at
8
-
-
microsomal preparation
8
-
Q8GGG1
phosphate buffer
8
-
-
assay at
8
-
-
assay at
additional information
-
-
value of pH-optimum for several enzyme isoforms
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
7.2
O05879, P0A650, P67208, P67210
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
15
-
-
assay at
23
-
-
assay at
25
-
-
assay at
27
-
-
assay at
30
-
-
low activity at 20C
30
-
-
assay at
30
-
-
assay at
30
-
Q2KP14
assay at
37
-
Q6ZPD8, Q96PD7
assay at; assay at
additional information
-
-
assay at various temperatures from 24C to 40C
additional information
-
-
assay at various temperatures from 24C to 40C
additional information
-
-
assay at various temperatures from 24C to 40C
additional information
-
-
assay at various temperatures from 24C to 40C
additional information
-
-
assay at various temperatures from 24C to 40C
additional information
-
-
assay at various temperatures from 24C to 40C
additional information
-
-
assay at various temperatures from 24C to 40C
additional information
-
-
assay at various temperatures from 24C to 40C
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
50
-
about 70% of maximal activity at 20C, about half-maximal activity at 50C
40
45
Q8GGG1
-
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8.1
-
B1NM15, B1NM16
calculated
8.2
-
B1NM15, B1NM16
calculated
9.05
-
Q8GGG1
calculated
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
3T3-L1 fibroblasts differentiated into adipocytes
Manually annotated by BRENDA team
-
isolated from parametrial adipose
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
increase of enzyme expression in diabetic animals
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
storage seed oil is mobilized into the cotyledons from endosperm
Manually annotated by BRENDA team
Q2KP14
developing
Manually annotated by BRENDA team
Arachis hypogaea JL 24
-
developing
-
Manually annotated by BRENDA team
-
microspore-derived
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
Q0QJH9, Q0QJI1, -
;
Manually annotated by BRENDA team
O75907, Q96PD7
;
Manually annotated by BRENDA team
-
leukemia, HL-60 cell line
Manually annotated by BRENDA team
-
small intestinal epithelium, decrease of specific activity from proximal to distal small intestine
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
Q9FNA9
very low level
Manually annotated by BRENDA team
-
DGAT2 is 18fold more highly expressed in seeds than in leaves and shows temporal specific expression during seed development, while DGAT1 shows little dfference in expression in seeds versus leaves, overview
Manually annotated by BRENDA team
Q0QJH9, Q0QJI1, -
;
Manually annotated by BRENDA team
-
very low expression level
Manually annotated by BRENDA team
Q9DCV3, Q9Z2A7
;
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
;
-
Manually annotated by BRENDA team
-
dehydration is an effective method of storage of samples destined for estimation of the microsomal enzyme activity
Manually annotated by BRENDA team
-
very low expression level
Manually annotated by BRENDA team
Zea mays Mo17
-
-
-
Manually annotated by BRENDA team
Q9FNA9
developing seed
Manually annotated by BRENDA team
-
developmenting, maximal activity at 8-14 days after flowering during rapid lipid accumulation, overview
Manually annotated by BRENDA team
Q9XGR5, Q9XGV4
developing oilseed; developing oilseed
Manually annotated by BRENDA team
Cuphea sp.
-
-
Manually annotated by BRENDA team
-
developing
Manually annotated by BRENDA team
-
germinating, enzyme expression throughout seed germination at levels considerably enhanced from that in the dormant seed, no expression in immature seed, expression profile, overview
Manually annotated by BRENDA team
-
DGAT2 is 18fold more highly expressed in seeds than in leaves and shows temporal specific expression during seed development, while DGAT1 shows little dfference in expression in seeds versus leaves, overview
Manually annotated by BRENDA team
Q0QJH9, Q0QJI1, -
developing; developing, high expression of DGAT2; developing, high expression of DGAT2
Manually annotated by BRENDA team
Q4U3T8, Q4U3T9
;
Manually annotated by BRENDA team
Q8RX96, -
gene is expressed exclusively in developing seeds
Manually annotated by BRENDA team
B1NM15, B1NM16
;
Manually annotated by BRENDA team
Zea mays Mo17
-
maturating
-
Manually annotated by BRENDA team
-
increase of enzyme expression in diabetic animals
Manually annotated by BRENDA team
Q6ZPD8, Q96PD7
predominant expression of DC3
Manually annotated by BRENDA team
-
high expression level
Manually annotated by BRENDA team
-
increase of enzyme expression in diabetic animals
Manually annotated by BRENDA team
additional information
-
distribution, overview
Manually annotated by BRENDA team
additional information
Q9DCV3
expression analysis; expression analysis
Manually annotated by BRENDA team
additional information
Q0QJH9, Q0QJI1, -
DGAT1 is expressed at similar levels in various organs, overview; DGAT1 is expressed at similar levels in various organs, whereas DGAT2 is strongly induced in developing seeds at the onset of oil biosynthesis, overview; DGAT2 is strongly induced in developing seeds at the onset of oil biosynthesis, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Q9SLD2
thylakoid and envelope membranes, not in the stroma
Manually annotated by BRENDA team
Arachis hypogaea JL 24
-
-
-
Manually annotated by BRENDA team
Q08650
both the N and C termini are oriented toward the cytosol and have different catalytic roles. A highly conserved motif, 129YFP131, and a hydrophilic segment exclusive to yeast DGAT2 reside in a long endoplasmic reticulum luminal loop following the first transmembrane domain and play an essential role in enzyme catalysis. The strongly conserved residue His195 within the motif HPHG, which may play a role in the active site of DGAT2, is likely embedded in the membrane
Manually annotated by BRENDA team
Q9DCV3
the endoplasmic reticulum targeting signal is present in the first transmembrane domain, TMD1, of isoform DGAT2
Manually annotated by BRENDA team
Q0QJH9, Q0QJI1, -
DGAT1 and DGAT2 are located in different subdomains, but contain similar C-terminal ER retrieval motifs
Manually annotated by BRENDA team
Q9DCV3
the interaction of isoform DGAT2 with lipid droplets is dependent on the C-terminus. Mutants, in which regions of the C-terminus are either truncated or specific regions are deleted, failed to co-localize with lipid droplets when cells are oleate loaded to stimulate triacylglycerol synthesis
-
Manually annotated by BRENDA team
-
yolk sac membrane
Manually annotated by BRENDA team
Q8GGG1
protein is mainly localised on the cytoplasmic site of the plasma membrane and on the surface of intracellular wax ester inclusions
Manually annotated by BRENDA team
-
DGAT2 is an integral membrane protein with both the N and C terminus oriented toward the cytosol, the loop between the two transmembrane domains of DGAT2is not exposed to the cytosol
Manually annotated by BRENDA team
Q9DCV3, Q9Z2A7
;
Manually annotated by BRENDA team
-
active site on cytoplasmic surface
-
Manually annotated by BRENDA team
-
active site on cytoplasmic surface; intrinsic membrane protein
-
Manually annotated by BRENDA team
Q9XGR5, Q9XGV4
;
-
Manually annotated by BRENDA team
Cuphea sp.
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
-
Manually annotated by BRENDA team
additional information
-
subcellular distribution
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
13280
-
-
N-terminal fragment, calculated DNA sequence
30800
-
O05879, P0A650, P67208, P67210
deduced from cDNA
36000
36500
-
SDS-PAGE
39500
-
-
DGAT2, calculated from amino acid sequence
39600
-
-
DGAT2A, calculated from amino acid sequence
40600
-
-
DGAT1A, calculated from amino acid sequence
44500
-
Q9DCV3
SDS-PAGE
47000
-
Q9Z2A7
SDS-PAGE
47100
-
Q9RIU8
deduced from cDNA
47400
-
Q8GGG1
deduced from cDNA
47400
-
Q826D7
deduced from cDNA
49300
-
O05879, P0A650, P67208, P67210
deduced from cDNA
49600
-
-
Wdh3563-2 deduced from cDNA
49900
-
-
Wdh3563-4 deduced from cDNA
50200
-
-
Wdh3563-1 deduced from cDNA
50700
-
O05879, P0A650, P67208, P67210
deduced from cDNA
50900
-
O05879, P0A650, P67208, P67210
deduced from cDNA
51600
-
-
Wdh3563-7 deduced from cDNA
51900
-
-
Wdh3563-3 deduced from cDNA
53000
-
-
SDS-PAGE
56900
-
-
DGAT1, calculated from amino acid sequence
60000
-
-
SDS-PAGE
94100
-
Q8GGG1
gel filtration
97000
-
Q8GGG1
gel filtration
100000
-
-
gel filtration
150000
-
-
gel filtration
1539000
-
-
delipidated enzyme, free of phospholipids, gel filtration
1843000
-
-
native enzyme monomer, gel filtration
2000000
-
-
gel filtration
additional information
-
-
MW 350000-370000, triacylglycerol synthetase complex, gel filtration
additional information
-
-
amino acid composition
additional information
-
Q4U3T8, Q4U3T9
56000-57000 Da; 56000-57000 Da
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
1 * 40800 + 2 * 28700 + 2 * 24500, complete structure of the native enzyme from soybean contains ten molecules of the pentameric enzyme, SDS-PAGE, amino acid analysis
?
-
x * 50000, SDS-PAGE
?
Q8GGG1
x * 51800, deduced from gene sequence
?
Q67C39
x * 59900, deduced from gene sequence, x * 60000, SDS-PAGE
?
-
x * 13278, recombinant poly-His-tagged N-terminal enzyme fragment comprising residues 1-116, sequence calculation
?
Q9DCV3, Q9Z2A7
x * 45000, recombinant DGAT2, SDS-PAGE; x * 47000, recombinant DGAT1, SDS-PAGE
?
Q2KP14
x * 41000, recombinant His-tagged enzyme, SDS-PAGE
?
-
x * 48900 (437 amino-acid residues), calculated
?
-
x * 54800, calculated
?
B1NM15, B1NM16
x * 59600, calculated; x * 60200, calculated
?
Arachis hypogaea JL 24
-
x * 41000, recombinant His-tagged enzyme, SDS-PAGE
-
dimer
Q8GGG1
2 * 38100, gel filtration
tetramer
-
4 * 50000, SDS-PAGE
trimer
-
-
homodimer
Q8GGG1
-
additional information
-
the native enzyme is presumably composed of 10 identical monomers each consisting of 3 different subunits with a ratio of 1:2:2
additional information
-
analysis of self-associating properties of BnDGAT1(1116)His6 in an gel filtration approach, the recombinant N-terminal fragment associates to form tetramers
additional information
Q9DCV3
individual isoform DGAT2 subunits are capable of interacting as part of a multimeric complex. Multiple domains, both in the N and C termini, mediate subunit interaction
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
proteolytic modification
Q08650
proteolytic cleavage at the N-terminal region is involved in enzyme activation in the snf2 disruptant, which lacks a DNA-dependent ATPase that forms the SWI/SNF chromatin remodeling complex. Major cleavage site lies between residues Lys29 and Ser30
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
23
-
-
at least 15 min stable
25
-
-
labile above, gradual inactivation
28
-
-
stable below, about 30% loss of activity after 15 min
30
-
-
rapid loss of activity
37
-
-
t1/2: 5 min
37
-
-
t1/2: 8 min
40
-
-
20% loss of activity after 15 min
40
-
-
stable below
40
-
-
complete loss of activity after 30 min
42
-
-
t1/2: 1.5 min
45
-
-
t1/2: 10 min
48
-
-
t1/2: 1 min
50
-
-
t1/2: 15 min
65
-
-
inactivation after 15 min
65
-
Q8GGG1
total loss of activity at temperatures above 65C
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
bovine serum albumin stabilizes, decrease in activity with buffers such as bis-Tris, HEPES, morpholinoethanesulfonic acid
Q8GGG1
freezing causes loss of 40% of the activity
-
antichaotropic salts stabilize solubilized enzyme
-
asolectin, GSH, DTT do not stabilize
-
phosphate buffer, high concentration, stabilizes
-
MgCl2 accelerates loss of activity during storage at 4C, dilution increases stability, cholate-solubilized activity is more stable than deoxycholate-solubilized activity
-
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Acetone
-
aqueous, stable to
Acetone
-
stable
benzene
-
stable
diethyl ether
-
stable
hexane
-
stable
additional information
-
stable in diisobutyl ketone and carbon tetrachloride
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-70C, without significant loss of its activity
Q8GGG1
4C, 20% loss of activity after 120 h
-
-20C, 3 months
-
-20C, 30 days, microsomal preparation
-
0C, inactivation after 4 days in Tris-HCl buffer
-
0C, more than 50% of activity retained after 4 days in 0.25 M phosphate buffer
-
-15C, several months, microsomal preparation
-
-20C, t1/2: 2 weeks in the presence of PMSF
-
-80C, at least 24 h, deoxycholate solubilized enzyme
-
-80C, undiluted, several weeks, cholate solubilized enzyme
-
-85C, negligible loss of activity in microsomes after 3 months
-
4C, 20-30% loss of activity after 24 h, cholate solubilized enzyme, dilution restores activity
-
4C, about 65-85% loss of activity after 24 h, deoxycholate solubilized enzyme, partially restorable by dialysis against buffer lacking detergent
-
4C, inactivation after 24 h in the presence of 8 mM MgCl2, deoxycholate solubilized enzyme
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cation-exchange chromatography, hydrophobic interaction chromatography, anion-exchange chromatography
-
purified enzyme is obtained by cation exchange chromatography, hydrophobic interaction chromatography and subsequent ion exchange chromatography
Q8GGG1
recombinant soluble N-terminally His-tagged enzyme 19.2fold from Escherichia coli strain BL21(DE3) by cation exchange and nickel affinity chromatography
-
recombinant enzyme
Q8GGG1
native enzyme 1724fold from developing cotyldons by hydrophobic interaction and adsorption chromatography, followed by gel filtration and heparin affinity chromatography to homogeneity
Q2KP14
recombinant poly-His-tagged N-terminal fragment of the enzyme, i.e. BnDGAT1(1116)His6, from Escherichia coli strain BL21(DE3)
-
native enzyme from tissue culture partially by microsome preparation
-
homogeneity
-
native enzyme from cotyledons to near homogeneity
-
native enzyme partially by microsome preparation
-
solubilized by aqueous acetone
-
native enzyme from tissue culture partially by microsome preparation
-
native enzyme partially by microsome preparation
-
native intestinal enzyme
-
near homogeneity
-
partial, with 1-acylglycerolphosphorylcholine acyltransferase
-
partial; with Triton X-100, deoxycholate or cholate
-
solubilized, as part of triacyl synthetase complex
-
native enzyme partially by microsome preparation
-
MrDGAT2A and MrDGAT2B
-
native enzyme from the oil body fraction to homogeneity
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli
-
DNA and amino acid sequence determination and analysis
-
expression as soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
DGAT1, a single copy gene, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional overexpression in tobacco plants
Q9SLD2
expressed in Escherichia coli and Saccharomyces cerevisiae
Q93ZR6
mutants AS11 and ABX45, ABX45 germination is delayed
-
DGAT1, DNA and amino acid sequence determination and analysis
-
expression as His-tagged enzyme in Escherichia coli strain BL21 (DE3), phylogenetic analysis
Q2KP14
gene is cloned; gene is cloned
Q4U3T8, Q4U3T9
DGAT1, DNA and amino acid sequence determination and analysis, phylogenetic tree; DGAT2, DNA and amino acid sequence determination and analysis, DGAT2 is a truncated enzyme version, phylogenetic tree
Q9XGR5, Q9XGV4
expression of the poly-His-tagged N-terminal fragment of the enzyme, i.e. BnDGAT1(1116)His6, in Escherichia coli strain BL21(DE3), BnDGAT1(1116)His6 fails to react with protein in microsomal vesicles following treatment with proteinase K, and thus might be localized to the cytosolic side of the ER
-
randomly mutagenized libraries expressed in Saccharomyces cerevisiae
-
wild-type and N-terminal fragment
-
DNA and amino acid sequence determination and analysis, phylogenetic tree
-
expressed in Saccharomyces cerevisiae
-
expressed in Saccharomyces cerevisiae
-
gene DGAT, genetic organization, DNA and amino acid sequence dteermination and analysis
Q5GKZ7
chromosomal location, expression of the FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells and in COS-7 cells
-
DGATsv, splice variant, deletion of 101 residues from the C-terminus, enzymatically inactive
-
expressed in a baculovirus expression system
-
expressed in Saccharomyces cerevisiae; expressed in Saccharomyces cerevisiae
O75907
expression in baculoviral system; expression in baculoviral system
O75907, Q96PD7
expression in Sf9 cell
-
gene DC3, location of DC3 on the X chromosome at Xq13.1, DNA and amino acid sequence determination and analysis, expression analysis, expression in Saccharomyces cerevisiae; gene DGAT2, localization of DGAT2 at 11q13.5, DNA and amino acid sequence determination and analysis, expression analysis, expression in Saccharomyces cerevisiae
Q6ZPD8, Q96PD7
-
Q9Z2A7
DGAT1, functional expression of the FLAG-tagged enzyme in membranes of Spodoptera frugiperda Sf9 cells and in COS-7 cells, expression analysis; DGAT2, functional expression of the FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells, expression analysis
Q9DCV3
DGAT2
Q9DCV3
DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in insect cells
-
expressed in a baculovirus expression system
-
expression in HEK-293T cell
Q9DCV3
gene DGAT2, expression of FLAG-tagged wild-type and mutant enzyme in COS-7 cells
-
short-term hepatic overexpression of DGAT1 in mice using adenoviral vectors results in 2.0fold increases in the triglyceride content of liver, the increase in hepatic triglyceride content has no effect on the production rate of VLDL triglyceride or apoB; short-term hepatic overexpression of DGAT2 in mice using adenoviral vectors results in 2.4fold increases in the triglyceride content of liver, the increase in hepatic triglyceride content has no effect on the production rate of VLDL triglyceride or apoB
Q9DCV3, Q9Z2A7
expressed in Escherichia coli
-
expression in Mycobacterium smegmatis
P0A4V2
gene tgs1, expressionin Escherichia coli, the recombinant enzyme prefers C26 : 0-CoA for triglycerol accumulation
-
expressed in Escherichia coli; expressed in Escherichia coli; expressed in Escherichia coli; expressed in Escherichia coli
O05879, P0A650, P67208, P67210
DGAT1, DNA and amino acid sequence determination and analysis, phylogenetic tree
Q9SEG9
DGAT1, DNA and amino acid sequence determination and analysis
-
expressed in a baculovirus expression system
-
DGAT1, DNA and amino acid sequence determination and analysis, phylogenetic tree
Q67C39
gene DGAT1, DNA and amino acid sequence determination and analysis, expression analysis of DGAT1 and DGAT2, expression in Saccharomyces cerevisiae microsomes, phylogenetic analysis
-
expressed in Escherichia coli
Q826D7
expressed in Escherichia coli
Q9RIU8
MrDGAT2A and MrDGAT2B
-
expression of Myc-tagged DGAT1 and DGAT2 in Saccharomyces cerevisiae, co-expression with N- and C-terminally myc-tagged DGAT1 or DGAT2, and GFP-tagged DGAT2 in Nicotiana tabacum BY-2 cells in endoplasmic reticulum membranes oriented toward the cytosolic side; expression of Myc-tagged DGAT1 in Saccharomyces cerevisiae, co-expression with N- and C-terminally myc-tagged DGAT1; expression of Myc-tagged DGAT2 in Saccharomyces cerevisiae, co-expression with N- and C-terminally myc-tagged DGAT2, and GFP-tagged DGAT2 in Nicotiana tabacum BY-2 cells in endoplasmic reticulum membranes oriented toward the cytosolic side
Q0QJH9, Q0QJI1, -
expressed in Saccharomyces cerevisiae; expressed in Saccharomyces cerevisiae
B1NM15, B1NM16
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
mRNA level is remarkably higher in the old leaves than in young developing leaves
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D137A
-
highly conserved residue in acyltransferases, mutation does not result in a significant decrease of activity
G138A
-
highly conserved residue in acyltransferases, mutation does not result in a significant decrease of activity
H132L
-
highly conserved residue in acyltransferases, mutation results in strongly decreased activity
H132L/H133L
-
highly conserved residues in acyltransferases
D137A
Acinetobacter baylyi ADP1
-
highly conserved residue in acyltransferases, mutation does not result in a significant decrease of activity
-
G138A
Acinetobacter baylyi ADP1
-
highly conserved residue in acyltransferases, mutation does not result in a significant decrease of activity
-
H132L
Acinetobacter baylyi ADP1
-
highly conserved residue in acyltransferases, mutation results in strongly decreased activity
-
H132L/H133L
Acinetobacter baylyi ADP1
-
highly conserved residues in acyltransferases
-
K232A
Q8MK44
naturally occuring mutation, association with milk fatty acid composition
P178I
-
mutation produces a drastic reduction of the neutral lipids content (yeast complementation experiment)
P178S
-
in this position S is invariantly found for acyl-CoA:diacylglycerol acyltransferase 1 proteins in plants and animals, mutation P178S does not have an appreciable effect on the synthesis on triacylglycerol (yeast complementation experiment)
C87S
-
site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme
H161A
-
site-directed mutagenesis, the mutant shows about 50% reduced activity compared to the wild-type enzyme
H161A/P162G/H163A
-
site-directed mutagenesis, the mutant shows over 80% reduced activity compared to the wild-type enzyme
S126A
P0A4V2
no detectable activity
F71A
Q08650
mutant retains more than 40% of wild-type activity
H193A
Q08650
almost complete loss of activity
H193E/G196S
Q08650
mutation to corresponding motif found in plant, abolishes enzymic activity
H195A
Q08650
complete loss of activity
H133L
-
highly conserved residue in acyltransferases, residue essential for catalytic activity
additional information
Q8GGG1
expression of atfA in a quadruple mutant of Saccharomyces cerevisiae, lacking own DGAT and steryl ester synthase activites by disrupted DGA1, LRO1, ARE1 and ARE2, restores triacylglycerol but not steryl ester biosynthesis and results in the formation and accumulation of fatty acid ethyl and isoamyl esters, indicating that also eukaryotic systems are suitable hosts for atfA expression
H133L
Acinetobacter baylyi ADP1
-
highly conserved residue in acyltransferases, residue essential for catalytic activity
-
additional information
Q9SLD2
a recombinant acyl-CoA binding protein increased the activity of Arabidopsis thaliana DGAT1 expressed in insect cell culture by up to 70%, overexpression of AtDGAT1 cDNA from Arabidopsis does not complement the knocked-out acyl-CoA:cholesterol acyltransferases, ACAT, EC 2.3.1.26, in a yeast double-mutant
H163A
-
site-directed mutagenesis, the mutant shows over 80% reduced activity compared to the wild-type enzyme
additional information
Q9DCV3
DGAT2 overexpression also increases wax monoester synthase activity in intact cells; the wax diester synthase activity of DGAT1 helps to explain the deficiency of type II wax diesters in the fur lipids of Dgat-/- mice, DGAT1 deficiency also perturbs retinol metabolism in the livers of Dgat-/- mice, hepatic levels of unesterified retinol are increased in Dgat-/- mice challenged with high-retinol diets
additional information
Q9DCV3, Q9Z2A7
short-term overexpression of DGAT1 increases hepatic triglyceride but not VLDL triglyceride or apoB production, overview; short-term overexpression of DGAT2 increases hepatic triglyceride but not VLDL triglyceride or apoB production, overview
additional information
Q9DCV3
an isoform DGAT2 mutant lacking both its transmembrane domains still associates with membranes, but is absent from the endoplasmic reticulum and instead localizes to mitochondria. The mutant is still active and capable of interacting with lipid droplets to promote triacylglycerol storage. Mutants, in which regions of the C-terminus are either truncated or specific regions are deleted, fail to co-localize with lipid droplets when cells are loaded with oleate to stimulate triacylglycerol synthesis
P162G
-
site-directed mutagenesis, the mutant shows about 40% reduced activity compared to the wild-type enzyme
additional information
-
disruption of gene tgs1, i.e. Rv3130c, leads to drastically reduced triacylglycerol accumulation
L73A
Q08650
mutant retains more than 40% of wild-type activity
additional information
Q08650
construction of N- and C-terminal truncation mutants N1(DELTA1-62), N2 (DELTA1-33), C1 (DELTA374-418), C2 (DELTA391-418), C3 (DELTA413-418), C4 (DELTA413-418, 413::A6). Mutant N1 lacking the entire hydrophilic N terminus presents minimal activity while maintaining a substantial expression level. Removal of the first 33 amino acid residues in the N-terminus, mutant N2, results in minor decrease in enzyme activity. Deletion of the last six amino acid residues from the C-terminus, mutant C3, causes a decrease in the enzyme activity of more than 80%. Deletion of the whole C-terminus, mutant C1, completely abolishes the enzyme activity and has a substantial impact on the protein accumulation. Mutant C2 lacking about half of the C-terminus, exhibits a complete loss of activity. In mutant C4, the last six amino acid residues are replaced with six alanine residues. This mutant retains similar activity and expression levels to C3. Deletion of the first putative TMD between residues 70 and 91 also results in the total loss of activity
Y129A/F130A/P131A
Q08650
almost complete loss of activity
additional information
Q9RIU8
construction of a SCO0958 deletion mutant shows that the protein is responsible for biosynthesis of a significant amount of triacylglycerol
additional information
Q8RX96, -
expression of recombinant TmDGAT1 in the yeast H1246MATalpha quadruple mutant (DGA1, LRO1, ARE1, ARE2) restores the capability of the mutant host to produce triacylglycerols; in plant transformation studies, seed-specific expression of TmDGAT1 is able to complement the low TAG/unusual fatty acid phenotype of the Arabidopsis AS11 (DGAT1) mutant; overexpression of TmDGAT1 in wild-type Arabidopsis and high-erucic-acid rapeseed (HEAR) and canola Brassica napus results in an increase in oil content (3.5%-10% on a dry weight basis, or a net increase of 11%-30%); site-directed mutagenesis is conducted on six putative functional regions/motifs of the TmDGAT1 enzyme. Mutagenesis of a serine residue in a putative SnRK1 target site results in a 38%-80% increase in DGAT1 activity, and over-expression of the mutated TmDGAT1 in Arabidopsis results in a 20%-50% increase in oil content on a per seed basis
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
analysis
-
dehydration is an effective method of storage of samples destined for estimation of the microsomal enzyme activity
medicine
-
enzyme expression is decreased in psoriatic skin
medicine
-
increase in triglyceride accumulation in diet-induced hyperlipidemic, insulin-resistant animals corresponds with increase in microsomal enzyme activity. Isoform DGAT1 activity changes most in liver and adipose tissue, DGAT2 responses mainly in muscle and intestine
medicine
-
enzyme expression is increased in diabetic animals
drug development
-
the enzyme is a possible target for antilatency drugs
medicine
-
use of enzyme as target for antilacency drugs that prevent cells from surviving dormancy
biotechnology
Q8RX96, -
overexpression of mutated DGAT1 in Arabidopsis can be used to enhance oil content