Information on EC 2.3.1.20 - diacylglycerol O-acyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.3.1.20
-
RECOMMENDED NAME
GeneOntology No.
diacylglycerol O-acyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acyl-CoA + 1,2-diacyl-sn-glycerol = CoA + triacylglycerol
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Cutin, suberine and wax biosynthesis
-
-
diacylglycerol and triacylglycerol biosynthesis
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-
Glycerolipid metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
Palmitoyl-CoA and other long-chain acyl-CoAs can act as donors.
CAS REGISTRY NUMBER
COMMENTARY hide
9029-98-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ADP1
-
-
Manually annotated by BRENDA team
strain ADP1, formerly Acinetobacter calcoaceticus ADP1
-
-
Manually annotated by BRENDA team
strain JL 24
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Cuphea sp.
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
sunflower, var. Albena
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-
Manually annotated by BRENDA team
cvs. AC Emerson and Vimy
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-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
Zea mays Mo17
maize, Mo17
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-diacyl-sn-glycerol + erucoyl-CoA
1,2-diacyl-3-erucoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacyl-sn-glycerol + oleoyl-CoA
1,2-diacyl-3-oleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
1,2-diacylglycerol + erucoyl-CoA
1,2-diacyl-3-erucoylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + oleoyl-CoA
1,2-diacyl-3-oleoylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + palmitoyl-CoA
1,2-diacyl-3-palmitoylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-diacylglycerol + stearoyl-CoA
1,2-diacyl-3-stearoylglycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-dilinolein + alpha-linolenoyl-CoA
1,2-dilinoleoyl-3-alpha-linolenoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
?
1,2-dilinolein + linoleoyl-CoA
1,2,3-trilinoleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
?
1,2-dilinolein + oleoyl-CoA
1,2-dilinoleoyl-3-oleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
?
1,2-dilinolein + palmitoyl-CoA
1,2-dilinoleoyl-3-palmitoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
?
1,2-dilinolenin + alpha-linolenoyl-CoA
1,2-dilinolenoyl-3-alpha-linolenoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
?
1,2-dilinolenin + linoleoyl-CoA
1,2-dilinolenoyl-3-linoleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
?
1,2-dilinolenin + oleoyl-CoA
1,2-dilinolenoyl-3-oleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
?
1,2-dilinolenin + palmitoyl-CoA
1,2-dilinolenoyl-3-palmitoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
?
1,2-diolein + alpha-linolenoyl-CoA
1,2-dioleoyl-3-alpha-linolenoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
?
1,2-diolein + linoleoyl-CoA
1,2-dioleoyl-3-linoleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
?
1,2-diolein + oleoyl-CoA
1,2,3-trioleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
?
1,2-diolein + palmitoyl-CoA
1,2-dioleoyl-3-palmitoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
?
1,2-dioleoyl-sn-glycerol + oleoyl-CoA
1,2,3-trioleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-dioleoyl-sn-glycerol + oleoyl-CoA
triolein + CoA
show the reaction diagram
-
-
-
-
?
1,2-dioleoyl-sn-glycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
-
?
1,2-dioleoylglycerol + arachidonoyl-CoA
1,2-dioleoyl-3-arachidonoylglycerol + CoA
show the reaction diagram
-
about 8% of the activity with palmitoyl-CoA
-
-
?
1,2-dioleoylglycerol + linoleoyl-CoA
1,2-dioleoyl-3-linoleoylglycerol + CoA
show the reaction diagram
-
about 12% of the activity with palmitoyl-CoA
-
-
?
1,2-dioleoylglycerol + oleoyl-CoA
triolein + CoA
show the reaction diagram
1,2-dioleoylglycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoyl-glycerol + CoA
show the reaction diagram
1,2-dioleoylglycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoylglycerol + CoA
show the reaction diagram
1,2-dioleoylglycerol + stearoyl-CoA
1,2-dioleoyl-3-stearoylglycerol + CoA
show the reaction diagram
-
about 10% of the activity with palmitoyl-CoA
-
-
?
1,2-dipalmitoyl-rac-glycerol + palmitoyl-CoA
rac-tripalmitoylglycerol + CoA
show the reaction diagram
1,2-dipalmitoyl-rac-glycerol + palmitoyl-CoA
tripalmitoylglycerol + CoA
show the reaction diagram
-
-
-
?
1,2-dipalmitoyl-sn-glycerol + oleoyl-CoA
1,2-dipalmitoyl-3-oleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
?
1,2-dipalmitoyl-sn-glycerol + palmitoyl-CoA
tripalmitin + CoA
show the reaction diagram
-
-
-
?
1,2-dipalmitoyl-sn-glycerol + stearoyl-CoA
1,2-dipalmitoyl-3-stearoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
?
1,2-diricinoleoyl-sn-glycerol + ricinoleoyl-CoA
triricinolein + CoA
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-oleoyl glycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
show the reaction diagram
1-palmitoyl-2-oleoylglycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
show the reaction diagram
-
-
-
-
?
2,3-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
acyl-CoA + 1,2-didecanoylglycerol
CoA + 3-acyl-1,2-didecanoylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-dihexanoylglycerol
CoA + 3-acyl-1,2-dihexanoylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-dilauroylglycerol
CoA + 3-acyl-1,2-dilauroylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-dimyristoylglycerol
CoA + 3-acyl-1,2-dimyristoylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-dioctanoylglycerol
CoA + 3-acyl-1,2-dioctanoylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-dioleoylglycerol
CoA + 3-acyl-1,2-dioleoylglycerol
show the reaction diagram
acyl-CoA + 1,2-dipalmitin
CoA + 3-acyl-1,2-dipalmitoylglycerol
show the reaction diagram
-
no acceptor
-
-
?
acyl-CoA + 1,2-dipalmitoylglycerol
CoA + 3-acyl-1,2-dipalmitoylglycerol
show the reaction diagram
-
membrane bound 1,2-dipalmitoylglycerol
-
-
?
acyl-CoA + 1,3-dioleoylglycerol
CoA + 2-acyl-1,3-dioleoylglycerol
show the reaction diagram
acyl-CoA + 1-decanol
? + CoA
show the reaction diagram
at 48% of the activity with hexanol
-
-
?
acyl-CoA + 2,3-dioleoyl-sn-glycerol
CoA + 1-acyl-2,3-dioleoyl-sn-glycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 2-cyclohexylethanol
? + CoA
show the reaction diagram
at 45% of the activity with hexanol
-
-
?
acyl-CoA + 2-decanol
? + CoA
show the reaction diagram
at 39% of the activity with hexanol
-
-
?
acyl-CoA + 2-monoacylglycerol
CoA + diacylglycerol
show the reaction diagram
-
-
-
-
-
acyl-CoA + 2-oleoylglycerol
CoA + 3-acyl-2-oleoylglyerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 4-decanol
? + CoA
show the reaction diagram
at 15.6% of the activity with hexanol
-
-
?
acyl-CoA + cyclododecanol
? + CoA
show the reaction diagram
at 80% of the activity with hexanol
-
-
?
acyl-CoA + cyclohexandiol
? + CoA
show the reaction diagram
at 4.1% of the activity with hexanol
-
-
?
acyl-CoA + cyclohexanol
? + CoA
show the reaction diagram
at 32% of the activity with hexanol
-
-
?
acyl-CoA + cyclohexanone oxime
? + CoA
show the reaction diagram
at 5.2% of the activity with hexanol
-
-
?
acyl-CoA + glycerol
CoA + acylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + hexadecanol
? + CoA
show the reaction diagram
-
-
-
?
acyl-CoA + hexadecanol
CoA + ?
show the reaction diagram
-
-
-
-
?
acyl-CoA + lysophosphatidylcholine
CoA + acyllysophosphatidylcholine
show the reaction diagram
-
-
-
-
?
acyl-CoA + phenol
? + CoA
show the reaction diagram
at 4.1% of the activity with hexanol
-
-
?
acyl-CoA + phenylethanol
? + CoA
show the reaction diagram
at 99% of the activity with hexanol
-
-
?
acyl-CoA + rac-1,2-diacetylglycerol
CoA + 3-acyl-rac-1,2-diacetylglycerol
show the reaction diagram
-
no acceptor
-
-
?
acyl-CoA + rac-1,2-dibutyrylglycerol
CoA + 3-acyl-rac-1,2-dibutyrylglycerol
show the reaction diagram
-
no acceptor
-
-
?
acyl-CoA + rac-1,2-dioleoylglycerol
CoA + 3-acyl-rac-1,2-dioleoylglycerol
show the reaction diagram
acyl-CoA + sn-1,2-diolein
CoA + acyl-sn-1,2-diolein
show the reaction diagram
acyl-CoA + sn-2-monoolein
CoA + ?
show the reaction diagram
arachidonoyl-CoA + 1,2-diacylglycerol
CoA + 3-arachidonoyl-1,2-diacylglycerol
show the reaction diagram
butyryl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-butyrylglycerol
show the reaction diagram
decanoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-decanoylglycerol
show the reaction diagram
-
saturated fatty acyl-CoAs from C-8 to C-18 with decanoyl-CoA as the best
-
-
?
diolein + acyl-CoA
?
show the reaction diagram
diolein + hexacosanoyl-CoA
?
show the reaction diagram
-
-
-
-
?
diolein + oleoyl-CoA
triolein + CoA
show the reaction diagram
-
-
-
-
?
dipalmitin + acyl-CoA
?
show the reaction diagram
erucoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-erucoylglycerol
show the reaction diagram
erucoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 3-erucoyl-1,2-dioleoyl-sn-glycerol
show the reaction diagram
-
-
-
-
?
lauroyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-lauroylglycerol
show the reaction diagram
linoleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-linoleoylglycerol
show the reaction diagram
linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
1,2-dioleoyl-3-linoleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
-
?
linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 3-linoleoyl-1,2-dioleoylglycerol
show the reaction diagram
-
i.e. diolein
-
?
myristoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-myristoylglycerol
show the reaction diagram
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
show the reaction diagram
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + triolein
show the reaction diagram
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
show the reaction diagram
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
triolein + CoA
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoylglycerol
CoA + 1,2,3-trioleoylglycerol
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1,2-dipalmitoylglycerol
CoA + 3-oleoyl-1,2-dipalmitoylglycerol
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1,2-diricinoloylglycerol
CoA + 3-oleoyl-1,2-diricinoloylglycerol
show the reaction diagram
best substrate
-
-
?
oleoyl-CoA + 1,2-divernoloyl-sn-glycerol
CoA + 1,2-divernoloyl-3-oleoyl-sn-glycerol
show the reaction diagram
oleoyl-CoA and 1,2-dioleoyl-sn-glycerol are preferred substrates over vernoloyl-CoA and 1,2-divernoloyl-sn-glycerol
-
-
?
oleoyl-CoA + diolein
CoA + triolein
show the reaction diagram
oleoyl-CoA + sn-1,2-dioleoylglycerol
triolein + CoA
show the reaction diagram
oleoyl-CoA + sn-2-monooleoylglycerol
CoA + 1,2-dioleoyl-sn-glycerol
show the reaction diagram
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
show the reaction diagram
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-palmitoyl-sn-glycerol
show the reaction diagram
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-palmitoylglycerol
show the reaction diagram
palmitoyl-CoA + 1,2-dipalmitin
CoA + tripalmitin
show the reaction diagram
palmitoyl-CoA + 1,2-dipalmitoylglycerol
CoA + tripalmitin
show the reaction diagram
approximately 10fold lower level of diacylglycerol acyltransferase activity
-
-
?
palmitoyl-CoA + 1,2-distearin
CoA + 3-palmitoyl-1,2-distearoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,3-diolein
CoA + 1,3-dioleoyl-2-palmitoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1,3-dipalmitin
CoA + tripalmitin
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1-monoolein
CoA + ?
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1-monopalmitin
CoA + ?
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1-monostearin
CoA + ?
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + didecanoylglycerol
CoA + 1,2-didecanoyl-3-palmitoylglycerol
show the reaction diagram
-
i.e. dicaprin
-
-
?
palmitoyl-CoA + dihexanoylglycerol
CoA + dihexanoyl-palmitoylglycerol
show the reaction diagram
-
i.e. dicaproin
-
-
?
palmitoyl-CoA + dipalmitoylglycerol
CoA + tripalmitoylglycerol
show the reaction diagram
-
membrane-bound dipalmitoylglycerol
-
-
?
palmitoyl-CoA + sn-2-monooleoylglycerol
CoA + 1-palmitoyl-2-oleoylglycerol
show the reaction diagram
-
-
-
-
-
ricinoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
1,2-dioleoyl-3-ricinoleoyl-sn-glycerol + CoA
show the reaction diagram
-
-
-
-
?
ricinoleoyl-CoA + 1,2-dipalmitoyl-sn-glycerol
1,2-dipalmitoyl-3-ricinoleoyl-sn-glycerol + CoA
show the reaction diagram
-
prefers acyl acceptor 1,2-dioleoyl-sn-glycerol over 1,2-dipalmitoyl-sn-glycerol
-
-
?
sn-1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
sn-1,2-diacylglycerol + erucoyl-CoA
triacylglycerol + CoA
show the reaction diagram
-
i.e. a 22:1cisDELTA13-CoA
-
-
?
stearoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-stearoylglycerol
show the reaction diagram
stearoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-stearoylglycerol
show the reaction diagram
vernoloyl-CoA + 1,2-divernoloyl-sn-glycerol
CoA + trivernoloyl-sn-glycerol
show the reaction diagram
oleoyl-CoA and 1,2-dioleoyl-sn-glycerol are preferred substrates over vernoloyl-CoA and 1,2-divernoloyl-sn-glycerol
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
1,2-dioleoylglycerol + oleoyl-CoA
triolein + CoA
show the reaction diagram
-
-
-
-
?
1,2-dioleoylglycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoylglycerol + CoA
show the reaction diagram
1,2-diricinoleoyl-sn-glycerol + ricinoleoyl-CoA
triricinolein + CoA
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
acyl-CoA + 2-monoacylglycerol
CoA + diacylglycerol
show the reaction diagram
-
-
-
-
-
palmitoyl-CoA + sn-2-monooleoylglycerol
CoA + 1-palmitoyl-2-oleoylglycerol
show the reaction diagram
-
-
-
-
-
sn-1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activation, to some extent
Co2+
-
activation to some extent
NaCl
-
1.4fold stimulation at 0.5 M
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2alpha,3beta)-2,3-dihydroxyurs-12-en-28-oic acid
-
IC50: 0.0443 mM
(2E)-1-[2,4-dihydroxy-6-methoxy-3-[(2R)-5-methyl-2-(1-methylethenyl)hex-4-en-1-yl]phenyl]-3-(2,4-dihydroxyphenyl)prop-2-en-1-one
-
IC50: 0.0098 mM
(2E,4Z,8E)-N-[9-(3,4-methylenedioxyphenyl)-2,4,8-nonatrienoyl]piperidine
-
IC50: 0.0298 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
(2R,3R)-2-(2,4-Dihydroxy-phenyl)-3,7-dihydroxy-8-((R)-5-hydroxy-2-isopropenyl-5-methyl-hexyl)-5-methoxy-chroman-4-one
-
i.e. kushenol H, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.142 mM
(2R,3S)-2-(2,4-Dihydroxy-phenyl)-3,7-dihydroxy-8-((R)-5-hydroxy-2-isopropenyl-5-methyl-hexyl)-5-methoxy-chroman-4-one
-
i.e. kushenol K, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.250 mM
(trans-4-(4-[(5-benzyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
inhibitor displays good metabolic stability and high intestinal permeability values
(trans-4-(4-[(5-cyclopentylethyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
-
(trans-4-(4-[(5-cyclopentylmethyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
-
(trans-4-(4-[(5-[1-fluorobenzyl]-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
inhibitor displays good metabolic stability and high intestinal permeability values
(trans-4-(4-[(5-[2-chlorobenzyl]-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
inhibitor displays good metabolic stability and high intestinal permeability values
(trans-4-(4-[(5-[2-fluorobenzyl]-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
inhibitor displays good metabolic stability and high intestinal permeability values
1,2-diacyl-sn-glycerol
-
dicaprin, dimyristin, dipalmitin, high concentration
1-acyl-sn-glycero-3-phosphocholine
-
activation, at low concentrations, inhibition above 0.2 mM
1-Hexanol
-
-
1-O-Hexadecyl-2-oleoyl-sn-glycerol
-
competitive
1-O-Hexadecylpropanediol-3-phosphocholine
-
activation, low concentration, inhibitory at high concentration
1-[2,4-dihydroxy-3-(2-isopropenyl-5-methyl-hex-4-enyl)-6-methoxy-phenyl]-3-(2,4-dihydroxy-phenyl)-propenone
-
i.e. kuraridin, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.099 mM
2-(2,4-dihydroxy-phenyl)-7-hydroxy-8-(2-isopropenyl-5-methyl-hex-4-enyl)-5-methoxy-chroman-4-one
-
i.e. kurarinone, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.0109 mM
2-(2,4-dihydroxy-phenyl)-7-hydroxy-8-[2-(3-hydroxy-3-methyl-butyl)-3-methyl-but-3-enyl]-5-methoxy-chroman-4-one
-
i.e. kurarinol, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.0086 mM
2-Bromooctanoate
2-butanol
-
-
5,5'-dithio-bis(2-nitrobenzoic acid)
6,8-diprenylgenistein
-
IC50: 0.0067 mg/ml
7beta-(3-ethyl-cis-crotonoyloxy)-1alpha-(2-methylbutyryloxy)-3,14-dehydro-Z-notonipetranone
8-angeloyloxy-3,4-epoxy-bisabola-7(14),10-dien-2-one
8-prenylleutone
-
IC50: 0.015 mg/ml
acyl-CoA
-
C-10-CoA to C-16-CoA, high concentration
alpinumisoflavone
-
23% inhibition at 0.0125 mg/ml
amidepsine A
;
amidepsine B
;
amidepsine C
;
-
amidepsine D
;
ATP
-
ATP-dependent activity reduces activity in vitro by 30-40%
auriculatin
-
IC50: 0.0072 mg/ml
betulinic acid
bovine serum albumin
brachynereolide
-
IC50: 0.25 mM, above
Butyryl-CoA
-
-
Cetyltrimethylammonium bromide
-
-
cholate
CoA
above 50 mM, about 70% inhibition above 300 mM
crepidiaside C
-
IC50: 0.25 mM, above
cryptotanshinone
-
IC50: 0.0273 mM
dehydropipernonaline
-
noncompetitive inhibition, IC50: 0.0212 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
deoxycholate
derrone
-
IC50: 0.0151 mg/ml
erysenegalensein D
-
IC50: 0.0015 mg/ml
erysenegalensein N
-
28% inhibition at 0.0125 mg/ml
erysenegalensein O
-
IC50: 0.0011 mg/ml
ethanol
-
43% inhibition when 0.04 ml are added to reaction mixture
germanicol acetate
-
IC50: 0.25 mM, above
Gum arabic
-
-
-
HgCl2
-
90% at 0.03-0.05 mM, reversible by DTT
I-
-
substantial inhibition
iodoacetamide
-
15% inhibition at 1 mM, DTT protects
ixerin Y
-
IC50: 0.25 mM, above
KCl
-
500 mM, inhibits purified enzyme
kuraridin
kurarinone
lysophosphatidylcholine
-
activation at low concentrations, optimum at 0.075 mM, inhibitory above 0.2 mM
methyl ursolate
-
IC50: 0.0264 mM
Mn2+
-
73% inhibition at 2.5 mM
N-(4,5-dihydronaphtho[1,2-d]thiazol-2-yl)-2-(3,4-dimethoxy phenyl)acetamide
selective inhibitor of isoform DGAT2 when used at low concentrations; selective inhibitor of isoform DGAT2 when used at low concentrations
N-ethylmaleimide
n-octyl-beta-D-glucopyranoside
-
weak
niacin
oleanolic acid
-
IC50: 0.0317 mM
oleate
-
noncompetitive inhibition, IC50: 0.0545 mM
oleoyl-CoA
-
substrate inhibition
p-chloromercuribenzene sulfonate
-
complete inhibition, DTT protects
p-chloromercuribenzoate
-
complete inhibition, DTT protects
palmitoyl-CoA
-
high concentration
phenylpyropene A
-
-
phenylpyropene B
-
-
phenylpyropene C
-
noncompetitive
pipernonaline
-
IC50: 0.0372 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
piperrolein B
-
IC50: 0.0201 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
retrofractamide C
-
IC50: 0.9 mM, above, isolated from the extracts of fruits of Piper longum and Piper nigrum
roselipin 1a
;
roselipin 1B
;
roselipin 2A
;
roselipin 2B
;
sesquiterpenoids
-
weak inhibition
-
sodium taurocholate
-
-
sodiumdodecyl sulfate
Soybean trypsin inhibitor
;
-
sphingosine
-
-
Tergitol NP-40
-
microsomal preparation
Trifluoperazine
-
0.5 mM, in the presence of suboptimal phosphatidic acid concentration
Triton WR1339
-
-
Triton X-100
tussilagone
tussilagonone
Tween 20
xanthohumol
;
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-acyl-sn-glycero-3-phosphocholine
-
activation, at low concentrations, inhibition above 0.2 mM
1-acyl-sn-glycero-3-phosphoethanolamine
-
activation, to a lesser extent
1-O-Hexadecylpropanediol-3-phosphocholine
-
activation, low concentration, inhibitory at high concentration
ammonium sulfate
45 mM, 10% increase in activity
bovine serum albumin
DTT
-
activation, microsomal preparation
ethanol
-
highest activity when diacylglycerol is in ethanol
glucose
-
treatment of adipocytes with glucose increases DGAT1 mRNA
Insulin
-
treatment of adipocytes with insulin increases DGAT2 mRNA
-
lysophosphatidylcholine
-
activation at low concentrations, optimum at 0.075 mM, inhibitory above 0.2 mM
phosphatidic acid
-
-
phosphatidylcholine
-
from egg yolk
phosphocholine
-
from soy bean, activation
Phospholipid
Sucrose
-
treatment of cells with elevated sucrose levels increases DGAT activity
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.014
1,2-diolein
-
-
0.016 - 0.025
Butyryl-CoA
-
-
0.028 - 0.714
diolein
0.0057 - 0.0068
Hexanoyl-CoA
-
-
0.0046 - 0.667
oleoyl-CoA
0.006 - 0.39
palmitoyl-CoA
0.113
sn-1,2-diolein
-
with oleoyl-CoA
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.92
palmitoyl-CoA
Mycobacterium tuberculosis
P9WQP3
pH 7.0, 37C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.032
1-O-Hexadecyl-2-oleoyl-sn-glycerol
-
-
0.0054 - 0.0081
oleoyl-CoA
0.0104
phenylpyropene C
-
-
additional information
additional information
-
inhibition kinetics
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0443
(2alpha,3beta)-2,3-dihydroxyurs-12-en-28-oic acid
Rattus norvegicus
-
IC50: 0.0443 mM
0.0098
(2E)-1-[2,4-dihydroxy-6-methoxy-3-[(2R)-5-methyl-2-(1-methylethenyl)hex-4-en-1-yl]phenyl]-3-(2,4-dihydroxyphenyl)prop-2-en-1-one
Rattus norvegicus
-
IC50: 0.0098 mM
0.0298
(2E,4Z,8E)-N-[9-(3,4-methylenedioxyphenyl)-2,4,8-nonatrienoyl]piperidine
Rattus norvegicus
-
IC50: 0.0298 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
0.00036
(trans-4-(4-[(5-benzyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
Homo sapiens
-
pH not specified in the publication, 25C
0.000019
(trans-4-(4-[(5-cyclopentylethyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
Homo sapiens
-
pH not specified in the publication, 25C
0.000024
(trans-4-(4-[(5-cyclopentylmethyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
Homo sapiens
-
pH not specified in the publication, 25C
0.000045
(trans-4-(4-[(5-[1-fluorobenzyl]-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
Homo sapiens
-
pH not specified in the publication, 25C
0.00003
(trans-4-(4-[(5-[2-chlorobenzyl]-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
0.047 - 0.1607
7beta-(3-ethyl-cis-crotonoyloxy)-1alpha-(2-methylbutyryloxy)-3,14-dehydro-Z-notonipetranone
0.211 - 0.2944
8-angeloyloxy-3,4-epoxy-bisabola-7(14),10-dien-2-one
0.04 - 0.07
amidepsine A
0.03 - 0.06
amidepsine B
0.2
amidepsine C
Homo sapiens
O75907
-
-
0.02 - 0.03
amidepsine D
0.0096
betulinic acid
Rattus norvegicus
-
a lupane-type triterpenoid, isolated from methanolic extracts of Alnus hirsuta, noncompetitive inhibition, IC50: 0.0096 mM
0.25
brachynereolide
0.0273
cryptotanshinone
Rattus norvegicus
-
IC50: 0.0273 mM
0.0212
dehydropipernonaline
Rattus norvegicus
-
noncompetitive inhibition, IC50: 0.0212 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
0.25
germanicol acetate
0.0098
kuraridin
0.0109 - 0.0288
kurarinone
0.0264
methyl ursolate
Rattus norvegicus
-
IC50: 0.0264 mM
0.0085 - 0.0708
N-(4,5-dihydronaphtho[1,2-d]thiazol-2-yl)-2-(3,4-dimethoxy phenyl)acetamide
0.0317
oleanolic acid
Rattus norvegicus
-
IC50: 0.0317 mM
0.0545
oleate
Homo sapiens
-
noncompetitive inhibition, IC50: 0.0545 mM
0.0787
phenylpyropene A
Rattus norvegicus
-
-
0.0217
phenylpyropene B
Rattus norvegicus
-
-
0.01104
phenylpyropene C
Rattus norvegicus
-
-
0.0372
pipernonaline
Rattus norvegicus
-
IC50: 0.0372 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
0.0201
piperrolein B
Rattus norvegicus
-
IC50: 0.0201 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
0.9
retrofractamide C
Rattus norvegicus
-
IC50: 0.9 mM, above, isolated from the extracts of fruits of Piper longum and Piper nigrum
0.03
roselipin 1a
Homo sapiens
O75907
-
0.04
roselipin 1B
Homo sapiens
O75907
-
0.045
roselipin 2A
Homo sapiens
O75907
-
0.05
roselipin 2B
Homo sapiens
O75907
-
0.0188 - 0.0491
tussilagone
0.0992
tussilagonone
Rattus norvegicus
-
-
0.04
xanthohumol
Homo sapiens
O75907
;
0.0000014 - 0.001499
XP620
additional information
amidepsine C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0003
-
membrane fraction after insulin-starvation
0.00036
-
membrane fraction after carbohydrate-starvation
0.0024 - 0.038
-
-
0.00265
-
membrane fraction
0.00292
-
after KCl wash
0.0035 - 0.0134
-
transfected cells
0.00361
-
-
0.0038
-
submandibular gland
0.00391
-
parotid gland
0.0071
purified native enzyme
0.0073
-
after stimulation with cytosolic fraction
0.0098
-
microsomal fraction of embryo liver after 12 days of development
0.0225
-
microsomal fraction of embryo liver after 19 days of development
0.0264
-
microsomal fraction of embryo liver after 22 days of development
0.05
-
; above
0.0543
pH 7.4, 35C
0.119
-
-
0.169 - 0.354
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1
-
broad
6.6
-
-
6.75 - 7.25
-
-
7 - 9
-
broad
7.5
-
assay at
7.8
-
assay at
additional information
-
value of pH-optimum for several enzyme isoforms
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT