Cloned (Comment) | Organism |
---|---|
gene DGAT2, expression of FLAG-tagged wild-type and mutant enzyme in COS-7 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C87S | site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme | Mus musculus |
H161A | site-directed mutagenesis, the mutant shows about 50% reduced activity compared to the wild-type enzyme | Mus musculus |
H161A/P162G/H163A | site-directed mutagenesis, the mutant shows over 80% reduced activity compared to the wild-type enzyme | Mus musculus |
H163A | site-directed mutagenesis, the mutant shows over 80% reduced activity compared to the wild-type enzyme | Mus musculus |
P162G | site-directed mutagenesis, the mutant shows about 40% reduced activity compared to the wild-type enzyme | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | DGAT2 is an integral membrane protein with both the N and C terminus oriented toward the cytosol, the loop between the two transmembrane domains of DGAT2is not exposed to the cytosol | Mus musculus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-1,2-diacylglycerol + acyl-CoA | Mus musculus | the final step in triacylglycerol synthesis is catalyzed by the acyl-CoA:diacylglycerol acyltransferase enzymes, DGAT1 and DGAT2 | triacylglycerol + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acyl-CoA + 1,2-diacyl-sn-glycerol = CoA + triacylglycerol | the conserved sequence 161HPHG164 is important for activity | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-1,2-diacylglycerol + acyl-CoA | the final step in triacylglycerol synthesis is catalyzed by the acyl-CoA:diacylglycerol acyltransferase enzymes, DGAT1 and DGAT2 | Mus musculus | triacylglycerol + CoA | - |
? | |
sn-1,2-diacylglycerol + acyl-CoA | the enzyme contains a neutral lipid binding sequence 80FLVLGVAC87 residing in the first transmembrane domain | Mus musculus | triacylglycerol + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acyl-CoA:diacylglycerol acyltransferase-2 | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |