6.3.5.3: phosphoribosylformylglycinamidine synthase

This is an abbreviated version!
For detailed information about phosphoribosylformylglycinamidine synthase, go to the full flat file.

Word Map on EC 6.3.5.3

6.3.5.3

purine

glutaminase

tegument

gammaherpesvirus

amidophosphoribosyltransferase

aminoimidazole

purinosome

acivicin

medicine

Reaction

N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide

2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine

Synonyms

2-Formamido-N-ribosylacetamide 5'-phosphate:L-glutamine amido-ligase (adenosine diphosphate), 5'-phosphoribosylformyl glycinamidine synthetase, FGAM, FGAM synthase, FGAM synthetase, FGAM-synthetase, FGAMS, FGAR amidotransferase, FGAR-AT, FGARAT, formylglycinamide ribonucleotide amidotransferase, formylglycinamide ribonucleotide amidotransferase complex, Formylglycinamide ribonucloetide amidotransferase, Formylglycinamide ribotide amidotransferase, Formylglycinamide ribotide synthetase, formylglycinamide synthetase, LT2, mPurL, ORF75c, PFAS, phosphoribosylformylglycinamidine synthase II, Phosphoribosylformylglycinamidine synthetase, Phosphoribosylformylglycineamidine synthetase, Pur4, PurL, PurS, smPurL, StPurL, Synthetase, phosphoribosylformylglycinamide, TmPurL

ECTree

6 Ligases

6.3 Forming carbon-nitrogen bonds

6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor

6.3.5.3 phosphoribosylformylglycinamidine synthase

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Results	in table
75581	AA Sequence
2	Application
1	CAS Registry Number
13	Cloned(Commentary)
1	Cofactor
12	Crystallization (Commentary)
40	Disease/ Diagnostics
2	General Information
3	General Stability
26	Inhibitors
21	KM Value [mM]
4	Localization
13	Metals/Ions
11	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
38	Organism
8	Pathway
42	PDB ID
5	pH Optimum
3	pH Range
1	pH Stability
12	Protein Variants
9	Purification (Commentary)
7	Reaction
2	Reaction Type
38	Reference
27	Source Tissue
6	Specific Activity [micromol/min/mg]
1	Storage Stability
42	Substrates and Products (Substrate)
4	Subunits
50	Synonyms
1	Systematic Name
2	Temperature Stability [°C]
2	Turnover Number [1/s]

Cloned

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Cloned on EC 6.3.5.3 - phosphoribosylformylglycinamidine synthase

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4 entries

expressed in Escherichia coli BL21DE3-834 (gold) cells

Salmonella enterica subsp. enterica serovar Typhimurium

P74881

728631

expression in Escherichia coli

Thermotoga maritima

Q9X0X3

690985

expression in Escherichia coli B834(DE3)

2 entries

MTH169 gene amplified by PCR using Mth genomic DNA as template, cloned into pET15b vector and expressed in Escherichia coli BL21 (DE3)

Methanothermobacter thermautotrophicus

653851

P-ADE5 gene cloned from a genomic library of Pichia methanolica by the ade6 mutation complementation in the recipient Saccharomyces cerevisiae strain

Ogataea methanolica

653876

purL gene encoded aminator subunit, purQ gene encoded glutamine amide transfer subunit

Bacillus subtilis

1259

PurS, smPurL, and PurQ are separately expressed in Escherichia coli

Bacillus subtilis

661076

yexA gene cloned and expressed in Escherichia coli

Bacillus subtilis

653151