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Literature summary for 6.3.5.3 extracted from

  • Schendel, F.J.; Mueller, E.; Stubbe, J.
    Formylglycinamide ribonucleotide synthetase from Escherichia coli: cloning, sequencing, overproduction, isolation, and characterization (1989), Biochemistry, 28, 2459-2471.
    View publication on PubMed
Search for more literature for 6.3.5.3

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
albizziin
-
 Escherichia coli
Glu
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 L-Gln 5'-phosphoribosylformylglycinamidine Escherichia coli
0.0468
-
 (-)carbocyclic-5'-phosphoribosylformylglycinamidine
-
 Escherichia coli
1
-
 NH4+
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ maximal activity at 10 mM K+ Escherichia coli
Mg2+ maximal activation at 20 mM Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
141418
-
 x * 141418, calculation from nucleotide sequence Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.15
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O
-
 Escherichia coli ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu
-
 ?
ATP + 5'-phosphoribosylformylglycinamide + NH4+ + H2O
-
 Escherichia coli ADP + phosphate + 5'-phosphoribosylformylglycinamidine
-
 ?
ATP + carbocyclic-5'-phosphoribosylformylglycinamide + L-Gln + H2O (+/-)carbocyclic-5'-phosphoribosylformylglycinamide and (-)5'-phosphoribosylformylglycinamide at 10% of the activity relative to 5'-phosphoribosylformylglycinamide Escherichia coli ADP + phosphate + carbocyclic-5'-phosphoribosylformylglycinamidine + L-Glu
-
 ?

Subunits

Subunits Comment Organism
? x * 141418, calculation from nucleotide sequence Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 NH4+
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8 about 50% of maximal activity at pH 6.2 and 8.0 Escherichia coli