Literature summary for 6.3.5.3 extracted from

Batra, R.; Christendat, D.; Edwards, A.; Arrowsmith, C.; Tong, L.
Crystal structure of MTH169, a crucial component of phosphoribosylformylglycinamidine synthetase (2002), Proteins Struct. Funct. Genet., 49, 285-288.

Search for more literature for 6.3.5.3

Cloned(Commentary)

Cloned (Comment)	Organism
MTH169 gene amplified by PCR using Mth genomic DNA as template, cloned into pET15b vector and expressed in Escherichia coli BL21 (DE3)	Methanothermobacter thermautotrophicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
crucial component MTH169, space group P4(3)22, unit cell parameters a : 53.8 A, c : 142.7 A	Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5'-phosphoribosylformylglycinamide + L-glutamine + ATP	Methanothermobacter thermautotrophicus	fourth step in the de novo purine biosynthetic pathway	phosphoribosylformylglycinamidine + L-glutamate + ADP + phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Methanothermobacter thermautotrophicus	-	thermophilic archaeon	-

Purification (Commentary)

Purification (Comment)	Organism
-	Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5'-phosphoribosylformylglycinamide + L-glutamine + ATP	-	Methanothermobacter thermautotrophicus	phosphoribosylformylglycinamidine + L-glutamate + ADP + phosphate	-	r
5'-phosphoribosylformylglycinamide + L-glutamine + ATP	fourth step in the de novo purine biosynthetic pathway	Methanothermobacter thermautotrophicus	phosphoribosylformylglycinamidine + L-glutamate + ADP + phosphate	-	r

Synonyms

Synonyms	Comment	Organism
FGAM synthetase	-	Methanothermobacter thermautotrophicus
Phosphoribosylformylglycinamidine synthetase	-	Methanothermobacter thermautotrophicus

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Release 2023.1 (January 2023)