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6.3.4.4: adenylosuccinate synthase

This is an abbreviated version!
For detailed information about adenylosuccinate synthase, go to the full flat file.

Word Map on EC 6.3.4.4

Reaction

GTP
+
IMP
 +
L-aspartate
=
GDP
 +
phosphate
 +
N6-(1,2-dicarboxyethyl)-AMP

Synonyms

adenosylsuccinate synthase, adenosylsuccinate synthetase, adenylo-succinate synthetase, Adenylosuccinate synthase, Adenylosuccinate synthetase, adenylosuccinate synthetase 1, AdSS, AdSS1, AdSS2, AdSSL1, AMPSase, AMPsS, Arabdss, AS-synthetase, ASS, IMP--aspartate ligase, IMP-aspartate ligase, mouse muscle synthetase, PfAdSS, purA, Succino-AMP synthetase, Succinoadenylic kinosynthetase, Wheatadss

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.4 adenylosuccinate synthase

Crystallization

Crystallization on EC 6.3.4.4 - adenylosuccinate synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
space group I23, cell constants a = b = c = 212.76 A
hanging drop vapor diffusion method, using 0.1 M bis-Tris propane pH 8, 0.2 M sodium bromide, 17% (w/v) polyethylene glycol 3350
-
crystal structure of guanine nucleotide complexes of adenylosuccinate synthetase
-
crystal structure of the enzyme complexed with GDP, IMP hadacidin, NO3-, and Mg2+
crystal structure of unligated enzyme
-
crystals grown by the method of hanging drops
entrapment of 6-thiophosphoryl-IMP in the active site of crystalline adenylosuccinate synthetase from Escherichia coli
-
hanging drop method, GDP-2'-deoxy-6-phosphoryl-IMP-hadacidin complex
analysis of high-resolution vibrational spectral fingerprints of each substrate and intermediate. The bound IMP is distorted toward its N1-deprotonated form even in the absence of any other ligands. Specific interactions between GTP and active-site amino acid residues result in large Raman shifts and contribute substantially to intrinsic binding energy. When both IMP and GTP are simultaneously bound, IMP is converted into an intermediate 6-phosphoryl inosine 5'-monophosphate. The intermediate complex is stable upon binding of the third ligand, L-aspartae analogue hadaicin. In the absence of hadaicin, 6-phosphoryl inosine 5'-monophosphate is quickly released from ADSS, is unstable in solution, and converts back into IMP. Hadaicin allosterically stabilizes ADSS through local conformational rearrangements
crystals grown by the method of hanging drops, space group P4(#)2(1)2, a = b = 69.93, c = 198.49
-
crystals grown by the method of hanging drops, space group P4(3)2(1)2, unit cell parameters a = b = 70.24 A, c = 199.14 A
hanging drop method, GDP-2'-deoxy-6-phosphoryl-IMP complex
enzyme-hydantocidin complex at 2.6 A resolution
-
space group C222(1), cell parameters a = 91.58 A, b = 117.12 A, c = 80.42A
sitting-drop vapour-diffusion method. Crystal belong to the trigonal space group P3(2)12, with unit-cell parameters a = b = 57.2, c = 107.9 A. There is a single molecule in the asymmetric unit
space group P4(1)2(1)2, cell constants a = b = 151.44 A, c = 91.94A