6.3.2.8: UDP-N-acetylmuramate-L-alanine ligase
This is an abbreviated version!
For detailed information about UDP-N-acetylmuramate-L-alanine ligase, go to the full flat file.
Word Map on EC 6.3.2.8
-
6.3.2.8
-
peptidoglycan
-
ligases
-
temperature-sensitive
-
cell-wall
-
drug development
- 6.3.2.8
- peptidoglycan
- ligases
-
temperature-sensitive
-
cell-wall
- drug development
Reaction
Synonyms
Alanine-adding enzyme, alr5065, L-Ala ligase, L-Alanine adding enzyme, Mur ligase, MurB/CVs, MurC, MurC ligase, MurC synthetase, Synthetase, uridine diphospho-N-acetylmuramoylalanine, UDP-acetylmuramoyl-L-alanine synthetase, UDP-MurNAc:L-Ala ligase, UDP-MurNAc:L-alanine ligase, UDP-N-acetylenolpyruvoylglucosamine reductase/UDP-N-acetylmuramate: L-alanine ligase, UDP-N-acetylmuramate-alanine ligase, UDP-N-acetylmuramate:L-alanine ligase, UDP-N-acetylmuramic acid L-alanine ligase, UDP-N-acetylmuramoyl-L-alanine synthetase, UDP-N-acetylmuramoyl:L-alanine ligase, UDP-N-acetylmuramoylalanine synthetase, UDP-N-acetylmuramyl:L-alanine ligase, UNAM:Ala ligase, Uridine 5´-diphosphate-N-acetylmuramyl-L-alanine synthetase, Uridine diphosphate N-acetylmuramate:L-alanine ligase, Uridine diphospho-N-acetylmuramoylalanine synthetase, Uridine-diphosphate-N-acetylmuramate:L-alanine ligase
ECTree
6.3.2.8 UDP-N-acetylmuramate-L-alanine ligaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 6.3.2.8 - UDP-N-acetylmuramate-L-alanine ligase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + dihydro-UDP-N-acetylmuramate + L-Ala
ADP + phosphate + dihydro-UDP-N-acetylmuramoyl-L-Ala
-
-
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + glycine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-glycine
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
ATP + UDP-N-acetyl-alpha-D-muramate + L-serine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-serine
ATP + UDP-N-acetyl-beta-D-muramate + L-alanine
ADP + phosphate + UDP-N-acetyl-beta-D-muramoyl-L-alanine
-
-
-
-
?
ATP + UDP-N-acetylglucosaminyl-enoylpyruvate + L-Ala
ADP + phosphate + UDP-N-acetylglucosaminyl-enoylpyruvoyl-L-Ala
-
-
-
-
?
ATP + UDP-N-acetylglucosyl-enolpyruvate + L-alanine
ADP + phosphate + UDP-N-acetylglucosyl-enolpyruvoyl-L-alanine
-
-
-
-
?
ATP + UDP-N-acetylmuramate + 2-amino-benzylpropionate
ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-benzylpropionate
-
-
-
-
?
ATP + UDP-N-acetylmuramate + 2-amino-ethylpropionate
ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-ethylpropionate
-
-
-
-
?
ATP + UDP-N-acetylmuramate + 2-amino-methylpropionate
ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-methylpropionate
-
-
-
-
?
ATP + UDP-N-acetylmuramate + 2-amino-N-butyric acid
ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-N-butyric acid
ATP + UDP-N-acetylmuramate + 2-amino-N-hydroxymethyl propionamide
ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-N-hydroxymethylpropionamide
-
-
-
-
?
ATP + UDP-N-acetylmuramate + 2-amino-propionamide
ADP + phosphate + UDP-N-acetylmuramoyl-2-aminopropionamide
-
-
-
-
?
ATP + UDP-N-acetylmuramate + 2-amino-tert-butylpropionate
ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-tert-butylpropionate
-
-
-
-
?
ATP + UDP-N-acetylmuramate + benzyl L-alaninate
ADP + phosphate + benzyl UDP-N-acetylmuramoyl-L-alanine
-
-
-
-
?
ATP + UDP-N-acetylmuramate + beta-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-beta-Ala
-
-
-
-
?
ATP + UDP-N-acetylmuramate + beta-chloro-L-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-beta-chloro-L-Ala
-
-
-
-
?
ATP + UDP-N-acetylmuramate + beta-cyano-L-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-beta-cyano-L-Ala
-
-
-
-
?
ATP + UDP-N-acetylmuramate + DL-propargylglycine
ADP + phosphate + UDP-N-acetylmuramoyl-DL-propargylglycine
-
-
-
-
?
ATP + UDP-N-acetylmuramate + ethyl L-alaninate
ADP + phosphate + ethyl UDP-N-acetylmuramoyl-L-alaninate
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-Cys
ADP + phosphate + UDP-N-acetylmuramoyl-L-Cys
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-Ile
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ile
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-Thr
ADP + phosphate + UDP-N-acetylmuramoyl-L-Thr
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-Val
ADP + phosphate + UDP-N-acetylmuramoyl-L-Val
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-vinylglycine
ADP + phosphate + UDP-N-acetylmuramoyl-L-vinylglycine
-
-
-
-
?
ATP + UDP-N-acetylmuramate + methyl L-alaninate
ADP + phosphate + methyl UDP-N-acetylmuramoyl-L-alaninate
-
-
-
-
?
ATP + UDP-N-acetylmuramate + N-(2-aminopropionyl)piperidine
ADP + phosphate + UDP-N-acetylmuramoyl-N-(2-aminopropionyl)piperidine
-
-
-
-
?
UTP + UDP-N-acetylmuramate + L-Ala
UDP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
about 11% of the activity relative to ATP
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + glycine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-glycine
-
reduced activity with glycine compared to L-alanine
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + glycine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-glycine
-
reduced activity with glycine compared to L-alanine
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
-
-
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
-
determination by phosphate detection using malachite green reagent
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
-
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
Nostoc sp. PCC 7120 = FACHB-418 SAG 25.82 / UTEX 2576
-
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
-
-
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
-
determination by phosphate detection using malachite green reagent
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
Pseudomonas aeruginosa Pae546
-
-
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
-
-
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
-
-
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + L-serine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-serine
-
reduced activity with L-serine compared to L-alanine
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + L-serine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-serine
-
reduced activity with L-serine compared to L-alanine
-
-
?
ATP + UDP-N-acetylmuramate + 2-amino-N-butyric acid
ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-N-butyric acid
-
-
-
-
?
ATP + UDP-N-acetylmuramate + 2-amino-N-butyric acid
ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-N-butyric acid
-
-
-
-
?
ATP + UDP-N-acetylmuramate + DL-Ser
ADP + phosphate + UDP-N-acetylmuramoyl-DL-Ser
-
-
-
-
?
ATP + UDP-N-acetylmuramate + DL-Ser
ADP + phosphate + UDP-N-acetylmuramoyl-DL-Ser
-
-
-
-
?
ATP + UDP-N-acetylmuramate + Gly
ADP + phosphate + UDP-N-acetylmuramoyl-Gly
-
-
-
-
?
ATP + UDP-N-acetylmuramate + Gly
ADP + phosphate + UDP-N-acetylmuramoyl-Gly
-
-
-
-
?
ATP + UDP-N-acetylmuramate + Gly
ADP + phosphate + UDP-N-acetylmuramoyl-Gly
-
-
-
?
ATP + UDP-N-acetylmuramate + glycine
ADP + phosphate + UDP-N-acetylmuramoyl-glycine
-
-
-
r
ATP + UDP-N-acetylmuramate + glycine
ADP + phosphate + UDP-N-acetylmuramoyl-glycine
-
-
-
r
ATP + UDP-N-acetylmuramate + glycine
ADP + phosphate + UDP-N-acetylmuramoyl-glycine
-
-
-
r
ATP + UDP-N-acetylmuramate + glycine
ADP + phosphate + UDP-N-acetylmuramoyl-glycine
-
-
-
-
?
ATP + UDP-N-acetylmuramate + glycine
ADP + phosphate + UDP-N-acetylmuramoyl-glycine
-
-
-
r
ATP + UDP-N-acetylmuramate + glycine
ADP + phosphate + UDP-N-acetylmuramoyl-glycine
-
-
-
r
ATP + UDP-N-acetylmuramate + glycine
ADP + phosphate + UDP-N-acetylmuramoyl-glycine
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-Ala
?
-
adds the first amino acid to the sugar moiety of the peptidoglycan precursor, catalyzing one essential step in cell wall biosynthesis
-
-
?
ATP + UDP-N-acetylmuramate + L-Ala
?
-
formation of a acylphosphate intermediate
-
-
?
ATP + UDP-N-acetylmuramate + L-Ala
?
-
enzyme activity is not in excess in the cell under normal growth conditions, but the amount is adjusted to the requirements of peptidoglycan synthesis
-
-
?
ATP + UDP-N-acetylmuramate + L-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
r
-
-
?
ATP + UDP-N-acetylmuramate + L-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
alpha- and beta-anomer of UDP-N-acetylmuramate
-
-
?
ATP + UDP-N-acetylmuramate + L-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
not active with D-Ala
-
?
ATP + UDP-N-acetylmuramate + L-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
accepts L-Ala, L-Ser and Gly as substrates, with a strong preference for L-Ala
-
-
?
ATP + UDP-N-acetylmuramate + L-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
-
-
r
ATP + UDP-N-acetylmuramate + L-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
-
-
r
ATP + UDP-N-acetylmuramate + L-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
MurC is essential for peptidoglycan biosynthesis and is responsible of the addition of the first residue L-alanine onto the nucleotide precursor UDP-MurNAc. It is regulated by the serine/threonine protein kinase PknA, overview
-
-
?
ATP + UDP-N-acetylmuramate + L-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
-
-
r
ATP + UDP-N-acetylmuramate + L-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
-
-
?
ATP + UDP-N-acetylmuramate + L-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
-
-
r
ATP + UDP-N-acetylmuramate + L-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
-
-
r
ATP + UDP-N-acetylmuramate + L-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
-
-
?
ATP + UDP-N-acetylmuramate + L-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-
?
ATP + UDP-N-acetylmuramate + L-serine
ADP + phosphate + UDP-N-acetylmuramoyl-L-serine
-
-
-
r
ATP + UDP-N-acetylmuramate + L-serine
ADP + phosphate + UDP-N-acetylmuramoyl-L-serine
-
-
-
r
ATP + UDP-N-acetylmuramate + L-serine
ADP + phosphate + UDP-N-acetylmuramoyl-L-serine
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-serine
ADP + phosphate + UDP-N-acetylmuramoyl-L-serine
-
-
-
-
?
ATP + UDP-N-acetylmuramate + L-serine
ADP + phosphate + UDP-N-acetylmuramoyl-L-serine
-
-
-
-
?
additional information
?
-
-
regulation by reversible phosphorylation, overview
-
-
?
additional information
?
-
-
the enzyme hydrolyzes ATP in absence of L-Ala. This L-Ala independent activity is dependent upon the concentration of both ATP and UDP-N-acetylmuramate
-
-
?
additional information
?
-
-
enzyme catalyzes an exchange reaction leading to the formation of 4% of the UDP-N-acetylmuramoyl-L-[14C]Ala with UDP-N-acetylmuramoyl-L-Ala, L-[14C]Ala, ADP and phosphate
-
-
?
additional information
?
-
-
the Mur ligases constitute a series of four ATP-dependent enzymes, MurC to Mur, that are responsible for the stepwise addition of the pentapeptide side chain onto the D-lactoyl group of the uridine diphosphate-N-acetylmuramic acid initially formed via MurA and MurB, MurC catalyzes the additon of the first amino acid L-alanine. All of the Mur ligases catalyze the formation of an amide or peptide bond through the same reaction mechanism via a tetrahedral intermediate, overview
-
-
?
additional information
?
-
-
MurC is able to incorporate glycine and L-Ala to UDP-MurNAc in Mycobacterium tuberculosis, the enzyme is highly specific for ATP and UDP-N-acetylmuramate, poor activity with ADP, GTP, CTP, UTP, and dTTP
-
-
?
additional information
?
-
-
MurC is able to incorporate glycine and L-Ala to UDP-MurNAc in Mycobacterium tuberculosis, the enzyme is highly specific for ATP and UDP-N-acetylmuramate, poor activity with ADP, GTP, CTP, UTP, and dTTP
-
-
?
additional information
?
-
-
in contrast to the UDP-N-acetylmuramate:L-alanine ligase (MurC) activity, the activity of the UDP-N-acetylenolpyruvoylglucosamine reductase (MurB, EC 1.3.1.98) cannot be detected in vitro
-
-
?
additional information
?
-
-
in contrast to the UDP-N-acetylmuramate:L-alanine ligase (MurC) activity, the activity of the UDP-N-acetylenolpyruvoylglucosamine reductase (MurB, EC 1.3.1.98) cannot be detected in vitro
-
-
?
