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Literature summary for 6.3.2.8 extracted from
- Overexpression, purification and characterization of UDP-N-acetylmuramyl:L-alanine ligase from Escherichia coli (1996), J. Bacteriol., 178, 906-910.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| murC gene is amplified by PCR and cloned with the expression vector pTrc99A | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-chloro-L-Ala | - |
Escherichia coli |  |
| 3-Cyano-L-Ala | - |
Escherichia coli | |
| Gly | - |
Escherichia coli | |
| KCl | inhibition above 100 mM | Escherichia coli | |
| L-Ser | - |
Escherichia coli | |
| NaCl | inhibition above 100 mM | Escherichia coli | |
| UDP-N-acetylmuramoyl-L-Ala | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.058 | - |
UDP-N-acetylmuramate | - |
Escherichia coli | |
| 0.087 | - |
L-Ala | - |
Escherichia coli | |
| 0.092 | - |
ATP | - |
Escherichia coli | |
| 0.173 | - |
beta-chloro-L-Ala | - |
Escherichia coli | |
| 1.99 | - |
L-Ser | - |
Escherichia coli | |
| 7.89 | - |
Gly | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | stimulation by 5-25 mM | Escherichia coli | |
| Mg2+ | divalent cation required, Mg2+ or Mn2+ | Escherichia coli | |
| Mg2+ | optimal concentration: 20 mM | Escherichia coli | |
| Mn2+ | divalent cation required | Escherichia coli | |
| Mn2+ | optimal concentration: 5 mM, reaching 50% of the activity measured in the presence of 20 mM MgCl2 | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
murC gene is amplified by PCR and cloned in the expression vector pTrc99A | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + UDP-N-acetylmuramate + beta-chloro-L-Ala | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-beta-chloro-L-Ala | - |
? | |
| ATP + UDP-N-acetylmuramate + DL-Ser | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-DL-Ser | - |
? | |
| ATP + UDP-N-acetylmuramate + Gly | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-Gly | - |
? | |
| ATP + UDP-N-acetylmuramate + L-Ala | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala | - |
? | |
| ATP + UDP-N-acetylmuramate + L-serine | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-serine | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.85 | - |
L-Ala | - |
Escherichia coli | |
| 11.1 | - |
beta-chloro-L-Ala | - |
Escherichia coli | |
| 11.4 | - |
L-Ser | - |
Escherichia coli | |
| 11.7 | - |
Gly | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10 | 7.5: about 70% of maximal activity, 10.0: about 95% of maximal activity | Escherichia coli |
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