Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
T362A/T365A/T51A
-
site-directed mutagenesis, the mutant shows impaired activity
T362A/T365A/T51A/T120A
-
site-directed mutagenesis, the mutant shows impaired activity
T362A/T365A/T51A/T120A/T167A
-
site-directed mutagenesis, the mutant shows impaired activity
T362A/T365A/T51A/T120A/T167A/T133A
-
site-directed mutagenesis, the mutant shows impaired activity
C230A
-
activity completely lost at 42°C
C426A
-
Km increased 3fold for ATP and 10fold for UDP-MurNAc and L-alanine, with respect to the wild-type
H199A
-
mutant enzymes K130A, H199A, N293A, N296A, and R327A lead to important variations of the Km value for one or more substrates
K130A
-
mutant enzymes K130A, H199A, N293A, N296A, and R327A lead to important variations of the Km value for one or more substrates
N293A
-
mutant enzymes K130A, H199A, N293A, N296A, and R327A lead to important variations of the Km value for one or more substrates
N296A
-
mutant enzymes K130A, H199A, N293A, N296A, and R327A lead to important variations of the Km value for one or more substrates
R327A
-
mutant enzymes K130A, H199A, N293A, N296A, and R327A lead to important variations of the Km value for one or more substrates
R151W
-
site-directed mutagenesis
Y246C
-
site-directed mutagenesis
R151W
-
site-directed mutagenesis
-
Y246C
-
site-directed mutagenesis
-
TOF-123
-
mutant enzyme TOF-95 is almost indistinguishable from wild type, mutant enzymes TOF-9 and TOF-123 have alanine-adding activity about 60% of the wild type at both 30 C and 43 C
TOF-9
-
mutant enzyme TOF-95 is almost indistinguishable from wild type, mutant enzymes TOF-9 and TOF-123 have alanine-adding activity about 60% of the wild type at both 30 C and 43 C
TOF-95
-
mutant enzyme TOF-95 is almost indistinguishable from wild type, mutant enzymes TOF-9 and TOF-123 have alanine-adding activity about 60% of the wild type at both 30 C and 43 C
additional information
-
mutation of the phosphorylation site threonine residues highly impairs enzyme activity
additional information
screening and identification of a transposon mutant impaired in diazotrophic growth, the mutant strain contains a transposon insertion at nucleotide position -476 relative to the annotated translational start site of gene alr5065. The mutant, designated C6, is unable to grow diazotrophically and displays a deficit in heterocyst differentiation, producing 4.5% heterocysts compared to the 9% produced by the wild type. Proper heterocyst production is restored in mutant C6 when the mutation is complemented with both alr5065 and alr5066, encoding enzymes MurC and MurB, as PpetE-murC/B (pPJAV328) in the presence of copper, but not when either gene is introduced individually, which suggests that alr5065 and alr5066 may be in an operon and the transposon insertion affectes the expression of both genes. After repeated rounds of subculturing, C6 loses viability and ceases to grow in culture. Generation of murC mutant strain UHM351, in which murC is inactivated by pPJAV309, phenotype, overview
additional information
Nostoc sp. PCC 7120 = FACHB-418 SAG 25.82 / UTEX 2576
-
screening and identification of a transposon mutant impaired in diazotrophic growth, the mutant strain contains a transposon insertion at nucleotide position -476 relative to the annotated translational start site of gene alr5065. The mutant, designated C6, is unable to grow diazotrophically and displays a deficit in heterocyst differentiation, producing 4.5% heterocysts compared to the 9% produced by the wild type. Proper heterocyst production is restored in mutant C6 when the mutation is complemented with both alr5065 and alr5066, encoding enzymes MurC and MurB, as PpetE-murC/B (pPJAV328) in the presence of copper, but not when either gene is introduced individually, which suggests that alr5065 and alr5066 may be in an operon and the transposon insertion affectes the expression of both genes. After repeated rounds of subculturing, C6 loses viability and ceases to grow in culture. Generation of murC mutant strain UHM351, in which murC is inactivated by pPJAV309, phenotype, overview
-