6.3.2.7: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase

This is an abbreviated version!
For detailed information about UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase, go to the full flat file.

Reaction

Synonyms

MuE, MurE, Synthetase, uridine diphospho-N-acetylmuramoylalanyl-D-glutamyllysine, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-lysine ligase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate: L-lysine ligase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:L-lysine ligase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate ligase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine synthetase, uridine 5'-diphospho-N-acetylmuramoyl L-alanyl-D-glutamate:lysine ligase, Uridine diphospho-N-acetylmuramoylalanyl-D-glutamyllysine synthetase

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.2 Acid—amino-acid ligases (peptide synthases)

                6.3.2.7 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase

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1	Activating Compound
1481	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
2	Cofactor
2	Crystallization (Commentary)
2	General Information
2	IC50 Value
48	Inhibitors
5	kcat/KM [mM/s]
20	KM Value [mM]
12	Metals/Ions
5	Natural Substrates/ Products (Substrates)
16	Organism
5	Pathway
4	PDB ID
5	pH Optimum
1	pH Range
2	pH Stability
1	pI Value
6	Protein Variants
6	Purification (Commentary)
1	Reaction
2	Reaction Type
14	Reference
4	Source Tissue
3	Specific Activity [micromol/min/mg]
1	Storage Stability
19	Substrates and Products (Substrate)
12	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 6.3.2.7 - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys

?

Lysinibacillus sphaericus

-

one step in the biosynthesis of bacterial cell walls

1270

-

-

?

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys

11 entries

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine

3 entries

additional information

?

-

3 entries

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys

Lysinibacillus sphaericus

-

-

985

-

985

?

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys

Lysinibacillus sphaericus

-

-

1033

-

1033

?

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys

Lysinibacillus sphaericus

-

-

1270

-

1270

?

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys

Lysinibacillus sphaericus 9602

-

-

985

-

985

?

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys

Lysinibacillus sphaericus 9602

-

-

1033

-

1033

?

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys

Staphylococcus aureus

-

-

1273

-

1273

?

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys

Staphylococcus aureus

-

-

1274

-

1274

?

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys

Staphylococcus aureus

-

-

1275

-

1275

?

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys

Staphylococcus aureus

-

-

671656, 714367

-

-

?

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys

Staphylococcus aureus Copenhagen

-

-

1275

-

1275

?

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys

Streptococcus pyogenes

-

-

661609

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine

Staphylococcus aureus

-

-

726560

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine

Staphylococcus aureus

Q2FZP6

the enzyme of the Gram-positive bacterium discriminates between L-lysine and D,L-diaminopimelic acid, the predominant amino acid that replaces L-lysine in Gram-negative peptidoglycan. Despite the absolute specificity for L-lysine, Staphylococcus aureus MurE binds this substrate relatively poorly. In vivo analysis and metabolomic data reveal that this is compensated for by high cytoplasmic L-lysine concentrations

727964

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine

Staphylococcus aureus

Q2FZP6

despite the absolute specificity for L-lysine, Staphylococcus aureus MurE binds this substrate relatively poorly. In vivo analysis and metabolomic data reveal that this is compensated for by high cytoplasmic L-lysine concentrations, substrate discrimination mechanism, overview. Glu460 is crucial for interaction with the epsilon-amino group of the L-lysine substrate

727964

-

-

?

additional information

?

-

Staphylococcus aureus

-

enzyme is specific for L-lysine and does not accept meso-diaminopimelic acid as substrate. The inverse and strict substrate specificities of the two MurE orthologues are responsible for the presence of exclusively mesodiaminopimelic acid and L-lysine at the third position of the peptide in the peptidoglycans of Escherichia coli and Staphylococcus aureus, respectively

714367

-

-

?

additional information

?

-

Staphylococcus aureus

Q2FZP6

the third amino acid added to the peptide stem of peptidoglycan is usually either meso-diaminopimelic acid (in most Gram-negative bacteria), EC 6.3.2.13, or L-lysine (in most Gram-positive bacteria), EC 6.3.2.7

727964

-

-

?

additional information

?

-

Staphylococcus aureus

-

the third amino acid added to the peptide stem of peptidoglycan is usually either meso-diaminopimelic acid (in most Gram-negative bacteria), EC 6.3.2.13, or L-lysine (in most Gram-positive bacteria), EC 6.3.2.7, although in some cases other amino acids are found at this position

726560

-

-

?