6.3.2.7: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase

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For detailed information about UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase, go to the full flat file.

Reaction

Synonyms

MuE, MurE, Synthetase, uridine diphospho-N-acetylmuramoylalanyl-D-glutamyllysine, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-lysine ligase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate: L-lysine ligase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:L-lysine ligase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate ligase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine synthetase, uridine 5'-diphospho-N-acetylmuramoyl L-alanyl-D-glutamate:lysine ligase, Uridine diphospho-N-acetylmuramoylalanyl-D-glutamyllysine synthetase

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.2 Acid—amino-acid ligases (peptide synthases)

                6.3.2.7 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase

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1	Activating Compound
1481	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
2	Cofactor
2	Crystallization (Commentary)
2	General Information
2	IC50 Value
48	Inhibitors
5	kcat/KM [mM/s]
20	KM Value [mM]
12	Metals/Ions
5	Natural Substrates/ Products (Substrates)
16	Organism
5	Pathway
4	PDB ID
5	pH Optimum
1	pH Range
2	pH Stability
1	pI Value
6	Protein Variants
6	Purification (Commentary)
1	Reaction
2	Reaction Type
14	Reference
4	Source Tissue
3	Specific Activity [micromol/min/mg]
1	Storage Stability
19	Substrates and Products (Substrate)
12	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Turnover Number [1/s]

Crystallization

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Crystallization on EC 6.3.2.7 - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant His-tagged enzyme in complex with UDP-MurNAc-Ala-Glu-Lys or with UDP-MurNAc-Ala-Glu-Lys and ADP, from 0.1 M Na-HEPES/MOPS, pH 7.5, 0.09 M nitrate phosphate sulfate mix, 30% w/v PEG550MME/PEG20K, method evaluation, X-ray diffraction structure determination and analysis at 1.8-1.9 A resolution, molecular replacement

Staphylococcus aureus

Q2FZP6

727964

crystals are obtained by the hanging-drop vapour-diffusion method at 291 K from solutions containing 25%(w/v) polyethylene glycol 2000 monomethylether, 0.2 M potassium thiocyanate, 0.1 M MES pH 6.5 in the presence of uridine 5'-diphospho-N-acetylmuramoyl alanyl glutamate (UDP-MurNAc-l-Ala-d-Glu) with and without 5'-adenylyl imidophosphate (AMP-PNP), a non-hydrolysable analogue of ATP. Crystals grown in the presence of two ligands belong to space group P1, with unit-cell parameters a = 68.4 A, b = 71.4 A, c = 74.8 A, alpha = 73.4, beta = 80.5, gamma = 72.3 A. Crystals grown in the presence of UDP-MurNAc-l-Ala-d-Glu alone belong to space group P2(1), with unit-cell parameters a = 71.1 A, b = 129.4 A, c = 74.6 A, beta = 106.3°

Streptococcus pneumoniae

-

660599