6.3.2.7: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase
This is an abbreviated version!
For detailed information about UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase, go to the full flat file.
Reaction
Synonyms
MuE, MurE, Synthetase, uridine diphospho-N-acetylmuramoylalanyl-D-glutamyllysine, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-lysine ligase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate: L-lysine ligase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:L-lysine ligase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate ligase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine synthetase, uridine 5'-diphospho-N-acetylmuramoyl L-alanyl-D-glutamate:lysine ligase, Uridine diphospho-N-acetylmuramoylalanyl-D-glutamyllysine synthetase
ECTree
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Engineering
Engineering on EC 6.3.2.7 - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase
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A409R
site-directed mutagenesis, the lytic phenotype observed upon overexpression of the wild-type murESa gene is replicated with the mutant
D406A
site-directed mutagenesis, the mutant shows very low or no activity in vivo
E460A
site-directed mutagenesis, the mutant shows very low or no activity in vivo
N407R
site-directed mutagenesis, the growth curve of N407R mutant is similar to that of wild-type murESa
P408A
site-directed mutagenesis, the lytic phenotype observed upon overexpression of the wild-type murESa gene is replicated with the mutant