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Literature summary for 6.3.2.7 extracted from

  • Triolo, T.A.; Chabin, R.M.; Pompliano, D.L.
    Cloning, expression and characterization of the Streptococcus pyogenes murE gene encoding a UDP-MurNAc-L-alanyl-D-glutamate: L-lysine ligase (2004), Enzyme Microb. Technol., 35, 300-308.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a glutathione-S-transferase/His12 fusion in Escherichia coli Streptococcus pyogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0205
-
L-Lys 23°C Streptococcus pyogenes
0.122
-
UDP-N-acetylmuramoyl-L-Ala-D-Glu 23°C Streptococcus pyogenes
0.125
-
ATP 23°C Streptococcus pyogenes

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys
-
Streptococcus pyogenes ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
-
Streptococcus pyogenes

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 40 15°C: about 30% of maximal activity, 40°C: about 45% of maximal activity Streptococcus pyogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9
-
ATP 23°C Streptococcus pyogenes
9
-
L-Lys 23°C Streptococcus pyogenes
9
-
UDP-N-acetylmuramoyl-L-Ala-D-Glu 23°C Streptococcus pyogenes

pI Value

Organism Comment pI Value Maximum pI Value
Streptococcus pyogenes calculation from amino acid sequence
-
6.02