Cloned (Comment) | Organism |
---|---|
expressed as a glutathione-S-transferase/His12 fusion in Escherichia coli | Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0205 | - |
L-Lys | 23°C | Streptococcus pyogenes | |
0.122 | - |
UDP-N-acetylmuramoyl-L-Ala-D-Glu | 23°C | Streptococcus pyogenes | |
0.125 | - |
ATP | 23°C | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys | - |
Streptococcus pyogenes | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
- |
Streptococcus pyogenes |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 40 | 15°C: about 30% of maximal activity, 40°C: about 45% of maximal activity | Streptococcus pyogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
ATP | 23°C | Streptococcus pyogenes | |
9 | - |
L-Lys | 23°C | Streptococcus pyogenes | |
9 | - |
UDP-N-acetylmuramoyl-L-Ala-D-Glu | 23°C | Streptococcus pyogenes |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptococcus pyogenes | calculation from amino acid sequence | - |
6.02 |