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6.1.1.26: pyrrolysine-tRNAPyl ligase

This is an abbreviated version!
For detailed information about pyrrolysine-tRNAPyl ligase, go to the full flat file.

Word Map on EC 6.1.1.26

Reaction

ATP
+
L-pyrrolysine
 +
tRNAPyl
=
AMP
 +
diphosphate
 +
L-pyrrolysyl-tRNAPyl

Synonyms

class II aminoacyl-tRNA synthetase, DhaPylSc, LysZ-RS, More, PylRS, PylS, PylSc, PylSn, pyrrolysyl-tRNA synthetase

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.26 pyrrolysine-tRNAPyl ligase

Cloned

Cloned on EC 6.1.1.26 - pyrrolysine-tRNAPyl ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DhaPylSc transformed into Escherichia coli BL21 (DE3)
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH10beta cells
expressed in HeLa cells
-
expression in Escherichia coli
expression of tRNAPyl in Chinese hamster ovary cells. ZLysRS-tRNAPyl pair and GRB2(Am111)-FLAG expressed in HEK293 c-18 cells
-
expression of variant pyrrolysyl-tRNA synthetase/tRNACUA Pyl pairs in Saccharomyces cerevisiae, tRNA mutant variants, overview
-
expression of wild-type and mutant PylRS in Escherichia coli
-
functional co-expression with tRNAPyl in Escherichia coli, the recombinant enzyme is active with substrate analogues N-epsilon-D-prolyl-L-lysine and N-epsilon-cyclopentyloxycarbonyl-L-lysine
-
gene pylS, DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as His-tagged enzyme
gene pylS, encoded in the pyl gene cluster
gene pylS, encoded in the pyl gene cluster, DNA and amino acid sequence analysis, recombinant expression in Escherichia coli, the pyl gene cluster codes all necessary enzymes for the biosynthesis of Pyl from Escherichia coli metabolites
gene pylS, expression as His6-tagged enzyme in Escherichia coli strain BL21 (DE3), addition of pyrrolysine to Escherichia coli cells expressing pylT, encoding tRNACUA, and pylS results in the translation of UAG in vivo as a sense codon, inability of PylS-His6 to synthesize lysyltRNACUA, co-expression with gene mtmB1
-
gene pylS, expression in Escherichia coli BL21(DE3) as His-tagged enzyme
-
gene pylS, expression of His-tagged wild-type and mutant enzymes in Escherichia coli BL21(DE3)
-
gene pylS, expression of operon pylTSBCD in Escherichia coli strain BL21 Tuner(DE3)
-
gene pylS, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene pylS, phylogenetic analysis and phylogeny of subclass IIc aaRSs, overview, expression of the N-terminally His-tagged catalytic domain of the enzyme in Escherichia coli
overexpression of the N-terminally truncated enzyme form PylRS(c270) as N-terminally His-tagged protein in Escherichia coli strain BL21(DE3), expression of selenomethionine-labeled enzyme in Escherichia coli strain B834(DE3)
-
recombinant expression of GFP-tagged mutant enzymes
-