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Literature summary for 6.1.1.26 extracted from

  • Yanagisawa, T.; Ishii, R.; Fukunaga, R.; Nureki, O.; Yokoyama, S.
    Crystallization and preliminary X-ray crystallographic analysis of the catalytic domain of pyrrolysyl-tRNA synthetase from the methanogenic archaeon Methanosarcina mazei (2006), Acta Crystallogr. Sect. F, 62, 1031-1033.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.26

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the N-terminally truncated enzyme form PylRS(c270) as N-terminally His-tagged protein in Escherichia coli strain BL21(DE3), expression of selenomethionine-labeled enzyme in Escherichia coli strain B834(DE3) Methanosarcina mazei

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged N-terminally truncated enzyme form PylRS(c270) in complex with an ATP analogue AMP-PNP, hanging drop vapour diffusion method, in 100 mM sodium cacodylate, pH 6.8, containing 0.25 M NaCl, 5 mM MgSO4 and 5% w/v PEG 4000, 20°C, hexagonal crystals, X-ray diffraction structure determination and analysis at 1.9-2.6 A resolution Methanosarcina mazei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 51000, full-length enzyme, SDS-PAGE, x * 33000, recombinant N-terminally truncated enzyme form PylRS(c270), SDS-PAGE Methanosarcina mazei
51000
-
 x * 51000, full-length enzyme, SDS-PAGE, x * 33000, recombinant N-terminally truncated enzyme form PylRS(c270), SDS-PAGE Methanosarcina mazei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-pyrrolysine + tRNAPyl Methanosarcina mazei
-
 AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina mazei
-
 a methanogenic archeon, strain JCM9314
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged N-terminally truncated enzyme form PylRS(c270) from Escherichia coli strain BL21(DE3) to homogeneity by affinity chromatography, hydrophobic interaction chromatography, and adsorption chromatography Methanosarcina mazei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-pyrrolysine + tRNAPyl
-
 Methanosarcina mazei AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, full-length enzyme, SDS-PAGE, x * 33000, recombinant N-terminally truncated enzyme form PylRS(c270), SDS-PAGE Methanosarcina mazei
More PylRS is mainly composed of two domains: the N-terminal RNA-binding domain and the C-terminal aaRS catalytic domain Methanosarcina mazei

Synonyms

Synonyms Comment Organism
PylRS
-
 Methanosarcina mazei
pyrrolysyl-tRNA synthetase
-
 Methanosarcina mazei

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Methanosarcina mazei