Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.1.1.26: pyrrolysine-tRNAPyl ligase

This is an abbreviated version!
For detailed information about pyrrolysine-tRNAPyl ligase, go to the full flat file.

Word Map on EC 6.1.1.26

Reaction

ATP
+
L-pyrrolysine
 +
tRNAPyl
=
AMP
 +
diphosphate
 +
L-pyrrolysyl-tRNAPyl

Synonyms

class II aminoacyl-tRNA synthetase, DhaPylSc, LysZ-RS, More, PylRS, PylS, PylSc, PylSn, pyrrolysyl-tRNA synthetase

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.26 pyrrolysine-tRNAPyl ligase

Purification

Purification on EC 6.1.1.26 - pyrrolysine-tRNAPyl ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
nickel-activated HiTrap column chromatography
purified by Ni-NTA chromatography and SDS gel electrophoresis
-
recombinant His-tagged enzyme from Escherichia coli BL21(DE3)
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged N-terminally truncated enzyme form PylRS(c270) from Escherichia coli strain BL21(DE3) to homogeneity by affinity chromatography, hydrophobic interaction chromatography, and adsorption chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli BL21(DE3)
-
recombinant His6-tagged PylS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant N-terminally His-tagged catalytic domain of the enzyme from Escherichia coli by two different steps of affinity chromatography, and gel filtration
resource Q column chromatography